白斑综合症病毒(WSSV)的实验室检测技术

主要依据90年代关于对虾病毒病的资料，就病原学检测、血清学检测以及基因组检测技术在WSSV感染检测的应用进行了比较系统的综述。     

光山凉亭金矿地球化学特征及找矿标志

凉亭金矿产于信阳群龟山组上部以绢云石英片岩、碳质绢云石英片岩为主的第四岩性段内，严格受东西向构造破碎带控制。破碎带蚀变强烈、种类繁多，与矿化关系密切的有硅化、绢云母化、褐铁矿化，深部有黄铁矿化等。矿化带原生晕表现为高背景值、强异常的特征。Ａｇ、Ａｓ、Ｐｂ、Ｍｏ、Ｂｉ标型元素与Ａｕ密切相关。本文着重讨论最佳指示元素在不同地层中分布规律、蚀变与矿化富集、原生异常与矿化（体）的关系等，同时建立指示元素的垂直和水平侧向分带序列。     

现代生态学研究的几大热点问题透视

自本世纪80年代以来，特别是近些年来，为适应全球环境问题与人类生存和发展的需要，生态学在研究方向、内容、尺度、方法上均有较大的转变，出现一些新的研究领域，主要包括全球变化、可持续发展、生物多样性、景观生态、退化生态、恢复与重建生态、湿地生态、生态工程、生态经济等较为热门的研究课题。本文简要对当今生态学热点问题加以概述，以此透视现代生态学的一些新的研究动向，旨在为生态工作者提供一些指导。     

The massive hot core associated with G31.41+0.31

We have used the IRAM Plateau de Bure Interferometer to observe the ground state and vibrationally excited lines of Methyl Cyanide towards the UCHII region G31.41+0.31. We also obtained a map of the continuum emission at a frequency of 110 GHz. We detect a hot molecular core in the emission both of methyl cyanide and of the 110 GHz continuum. We estimate a temperature of 200 K and a mass of 1000 M for this compact massive region. We also detect a velocity gradient or shift across the methyl cyanide core whose origin could be due to rotation.     

Assessment of Fatigue Strength of An Offshore Floating Production and Storage Unit

The procedure of assessment of structural fatigue strength of an offshore floating production and storage and offloading unit(FPSO) in this paper. The emphasis is placed on the long-term prediction of wave induced loading, the refined finite element model for hot spot stress calculation, the combination of stress components, and fatigue damage assessment based on S-N curve.     

Structure of the Malpelo Ridge (Colombia) from seismic and gravity modelling

Wide-angle and multichannel seismic data collected on the Malpelo Ridge provide an image of the deep structure of the ridge and new insights on its emplacement and tectonic history. The crustal structure of the Malpelo Ridge shows a 14 km thick asymmetric crustal root with a smooth transition to the oceanic basin southeastward, whereas the transition is abrupt beneath its northwestern flank. Crustal thickening is mainly related to the thickening of the lower crust, which exhibits velocities from 6.5 to 7.4 km/s. The deep structure is consistent with emplacement at an active spreading axis under a hotspot like the present-day Galapagos Hotspot on the Cocos-Nazca Spreading Centre. Our results favour the hypothesis that the Malpelo Ridge was formerly a continuation of the Cocos Ridge, emplaced simultaneously with the Carnegie Ridge at the Cocos-Nazca Spreading Centre, from which it was separated and subsequently drifted southward relative to the Cocos Ridge due to differential motion along the dextral strike-slip Panama Fracture Zone. The steep faulted northern flank of the Malpelo Ridge and the counterpart steep and faulted southern flank of Regina Ridge are possibly related to a rifting phase that resulted in the Coiba Microplate’s separation from the Nazca Plate along the Sandra Rift.     

套式PCR检测斑节对虾白斑症病毒(WSSV)

解剖患白斑症斑节对虾的鳃组织 ,制备成不同稀释倍数的模板 ,对其进行白斑症病毒 (WSSV)的 PCR扩增 ,结果显示所建立的套式 PCR的灵敏度大约为一步 PCR的 10 4 倍 ;对感染病毒后不同时期的斑节对虾进行 PCR检测 ,发现感染早期经一步 PCR检测为 WSSV阴性的样品 ,套式 PCR的检测结果为阳性 ;对发病虾塘中的几种甲壳类动物进行 PCR检测 ,发现经一步 PCR检测为阴性的长臂虾、秉氏厚蟹和褶痕相手蟹等宿主 ,套式 PCR的检测结果为阳性。表明所建立的 WSSV套式 PCR检测法较普通的一步 PCR有更大的应用价值和意义     

营养盐和微量元素与黄骅赤潮的相关性

本文主要通过１９８９～１９９２年河北省黄骅市沿海一带赤潮水质监测的结果，探讨赤潮发生期间营养盐及微量元素的含量变化与赤潮的关系。结果表明，营养盐的富集导致赤潮藻类的快速增殖，微量元素Ｆｅ和Ｍｎ的过量又是赤潮形成的诱发因子。     

Gravity and multichannel seismic reflection constraints on the lithospheric structure of the Canary Swell

Deep penetrating multichannel seismic reflection and gravity data have been used to study the lithospheric structure of the Canary Swell. The seismic reflection data show the transition from undisturbed Jurassic oceanic crust, away from the Canary Islands, to an area of ocean crust strongly modified by the Canary volcanism (ACV). Outside the ACV the seismic records image a well layered sedimentary cover, underlined by a bright reflection from the top of the igneous basement and also relatively continuous reflections from the base of the crust. In the ACV the definition of the boundary between sedimentary cover and igneous basement and the crust-mantle boundary remains very loose. Two-dimensional gravity modelling in the area outside the influence of the Canary volcanism, where the reflection data constrain the structure of the ocean crust, suggests a thinning of the lithosphere. The base of the lithosphere rises from 100 km, about 400 km west of the ACV, to 80 km at the outer limit of the ACV. In addition, depth conversion of the seismic reflection data and unloading of the sediments indicate the presence of a regional depth anomaly of an extension similar to the lithospheric thinning inferred from gravity modelling. The depth anomaly associated with the swell, after correction for sediment weight, is about 500 m. We interpret the lithospheric thinning as an indication of reheating of old Mesozoic lithosphere beneath the Canary Basin and along with the depth anomaly as indicating a thermal rejuvenation of the lithosphere. We suggest that the most likely origin for the Canary Islands is a hot spot.