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961.
四株鳖源致病性嗜水气单胞菌(Aeromonas hydrophila)的表型、分子鉴定及其毒力基因检测 总被引:2,自引:0,他引:2
采用生态毒理学、表观分类学及分子生物学方法,对具白底板病典型症状濒死中华鳖内脏中分离获得的4株病原菌开展了以致病性、表型分析、分子鉴定及毒力基因检测为内容的实验研究,结果表明:(1)4株病原菌均具致病性,致死力由大到小依次为ZHYYZ-2、ZHYYZ-4、ZHYYZ-1、ZHYYZ-3;(2)4株病原菌均为呈短杆状、具溶血活性的革兰氏阴性细菌,VITEK2型全自动细菌鉴定与药敏系统和ATBExpression型细菌鉴定与药敏智能系统均显示为嗜水气单胞菌;(3)ZHYYZ-1、ZHYYZ-2、ZHYYZ-3、ZHYYZ-4的16SrDNA序列长度分别为1460、1464、1466、1461,经Blast同源性检索表明它们所扩增的16SrDNA序列与GenBank数据库中登记的71株嗜水气单胞菌的相似性均为99%;(4)经PCR特异性检测,各实验菌均含有Aha、AHH、AerA和OMP。根据4株实验菌的表型和分子生物学特征,判定它们均为气单胞菌属的致病性嗜水气单胞菌。 相似文献
962.
963.
彩色全息显示中液晶空间光调制器位相调制偏差的矫正方法 总被引:2,自引:0,他引:2
采用三基色激光照明并结合时分复用或空间复用技术,可实现基于液晶空间光调制器(LC-SLM)的彩色全息显示. 但由于在不同激光波长入射条件下LC-SLM的位相调制特性曲线不同,难以同时满足多波长入射条件下2π线性位相调制的要求,致使彩色全息再现结果受到共轭像和零级斑的干扰. 针对该问题,本文通过实验测试获得LC-SLM在不同波长入射时的位相调制特性曲线,并分析了RGB激光器各分量的位相调制特性曲线非线性偏差和调制幅度偏差对多阶位相型傅里叶变换相息图再现效果的影响. 根据"查表法"建立了各波长入射条件下满足2 相似文献
964.
研究了激发态质子转移(ESPT)分子2-(2'-羟基苯基)苯并噻唑(HBT)在不同极性溶剂中的光开关行为,探讨了溶剂极性对HBT分子光开光效应的影响.揭示了光开关脉冲信号的形成原因,建立了基于光诱导HBT分子激发态非线性折射效应的皮秒全光开关的理论模型.根据对时间响应函数的理论计算和实验结果分析,确定了光开关脉冲信号下降前沿和上升后沿的形成机理以及影响因素,并提出了增强光开关信号下降前沿的关断深度,提升上升后沿的恢复速度的有效途径和方法.本文工作为制成皮秒量级关断,微秒甚至纳秒量级重新打开的快速全光开关器 相似文献
965.
LI Baoquan LI Xinzheng WANG Hongf WANG Jinbao HAN Qingxi DONG Chao MA Lin ZHANG Baolin 《海洋学报(英文版)》2010,29(6):73-82
Macrobenthic infauna and associated environmental factors influencing the benthic community in the eastern coastal region of Shandong Peninsula were analyzed in four seasonal surveys from January 2007 to October 2007 (30 stations in winter, 20 stations in other three seasons), in order to understand the community structure and the factors unfluencing the benthic distribution. PRIMER 6.0 and SPSS 15.0 software packages were adopted to analyze the environmental and macrobenthic data. The results show that there were 260 macrobenthic species in total collected from the research region. The composition of species is:Polychaeta (94 species), Crustacea (75), Mollusca (56) and Echinoderm (12), among which, only 23 species were common species in the cruises of every season. The dominant species varied from season to season; however, the polychaete species Paralacydonia paradoxa Fauvel and Echinoderm species Amphioplus japonicus (Matsumoto) were always present year-round. The abundance and biomass of the macrobenthos in the research region were variable from season to season. The results of CLUSTER and MDS analysis show that the similarities of macrobenthic structures between the stations were low; most of the similarities were at about 30% of similarity value, only two stations were up to 70%. In accordance with the similarity values of the macrobenthic structures, we divided the 20 stations into five groups by arbitrary similarity level of 30%. The ABC curve indicates that the marcofauna communities in the research region had not been disturbed massively, except two stations, SB1 and SB3. Ecologically, benthos were controlled by a combination of factors such as salinity, phytoplankton, zooplankton, SiO3-Si and temperature, and no single factor could be considered as an ecological master factor. 相似文献
966.
Kimberly K. Takagi Phaothep Cherdsukjai Izumi Mimura Yuka Yano Kanjana Adulyanukosol Makoto Tsuchiya 《Estuarine, Coastal and Shelf Science》2010
This study aimed to elucidate Dotilla myctiroides (H. Milne-Edwards) food resources and how the soldier crab influences the organic matter composition on the intertidal flat. A preliminary assessment of D. myctiroides size, sex, and population distribution was conducted to clarify the characteristics of the inhabiting population. Overall, D. myctiroides size and abundance was normal across the tidal flat area. Comparison of different sediment types from the soldier crab habitat (surface sediments, igloos, burrow lining, burrow pellets, feeding pellets, and feces) using the fatty acid (FA) analysis technique revealed D. myctiroides removes organic matter via the significantly lower contributions of polyunsaturated FAs, essential FAs, and dinoflagellates in the feeding pellets. Dotilla myctiroides tissue analysis of the hepatopancreas and hindgut in individuals that had been starved for 24 h confirmed not only the consumption of dinoflagellates, but diatoms and macroalgae as well. In addition, the FA metabolism within the digestive system implied the ability of the soldier crab to synthesize highly unsaturated FAs (HUFAs) such as 20:4n–6, 20:5n–3 and 22:6n–3. 相似文献
967.
Much of the research that concerns the impacts of management measures in the eastern Baltic cod fishery has focused on fish stock rather than understanding fishermen's attitudes towards regulations. Hence, there is little information available on fishermen's responses although they are the ones whom the regulations affect most profoundly. This study analyses the views of fishermen towards management measures with an emphasis on fishing closures (marine protected areas, MPAs). Swedish log-book data from 1996 to 2005 were used to describe MPA induced fishing effort displacements. Fishermen argued that MPAs have been inefficient in conservation of cod stock. The enlargement of Bornholm MPA in 2005 caused substantial effort displacement towards areas dominated by smaller sized fish. This contributed to the increased discarding of juvenile cod. Enlarged MPAs also intensified competition between different fleet segments and reallocated fishing areas. To reduce fishing mortality, fishermen suggested days-at-sea (effort) regulation and an effective landings control system for all fleets that exploit cod stocks in the Baltic Sea Main Basin. These measures would better motivate fishermen for mutual rule compliance, which is a prerequisite for a sustainable cod fishery. 相似文献
968.
Headland-bay beach (HBB) is one of the most prominent physiographic features on the oceanic margin of many countries in the world. Under the influence of a predominant swell, its curved periphery in natural environment may reach static equilibrium and remains stable without sediment supply from updrift and/or a riverain source within its own embayment. Coastal scientists and engineers have attempted to develop mathematical expressions to quantify this ideal bay shape since the 1940s. As the scenario with depleting sediment supply has become a common reality on many parts of the world coastline in more recent time, some coastal engineers have advocated a rational approach to mimicking the static bay shape found in nature in order to mitigate beach erosion as well as for coastal management. Nowadays, many useful applications have emerged since the publication of the parabolic bay shape equation (PBSE) developed for static equilibrium planform (SEP) in late 1980s. The advance in modern computer technologies and international collaboration has further facilitated the exchange of knowledge and applications of this static bay beach concept (SBBC). 相似文献
969.
The directional spreading of both the wavenumber and frequency spectra of finite-depth wind generated waves at the asymptotic depth limit are examined. The analysis uses the Wavelet Directional Method, removing the need to assume a form for the dispersion relationship. The paper shows that both the wavenumber and frequency forms are narrowest at the spectral peak and broaden at wavenumbers (frequencies) both above and below the peak. The directional spreading of the wavenumber spectrum is bi-modal above the spectral peak. In contrast, the frequency spectrum is uni-modal. This difference is shown to be the result of energy in the wind direction being displaced from the linear dispersion shell. A full parametric relationship for the directional spreading of the wavenumber spectrum is developed. The analysis clearly shows that typical dispersion relationships are questionable at high frequencies and that such effects can be significant. This result supports greater attention being focussed on the routine recording of wavenumber spectra, rather than frequency spectra. 相似文献
970.
The governments around the Baltic Sea have agreed on a new set of targets for nutrient load reductions. The major motive for this is new and better knowledge about the link between nutrient loads and water transparency in different parts of the sea. The Baltic Sea Action Plan (BSAP) defines target for transparency in different marine basins, the load reductions necessary to meet transparency targets and a scheme for the distribution of the abatement burden between countries adjacent to the sea. 相似文献