海蜇幼体环介导等温扩增快速检测方法的建立和应用

针对海蜇早期生活史阶段幼体种类鉴定困难的问题,建立一种快速、灵敏、操作简便的环介导等温扩增(LAMP)快速检测方法.根据海蜇的线粒体细胞色素C氧化酶Ⅰ基因(COⅠ)编码序列,设计LAMP引物,建立了海蜇幼体LAMP快速检测方法,并对LAMP反应体系、反应温度和反应时间进行了优化.结果表明,LAMP方法对海蜇目的基因的最小检测限为0.1 ng DNA.该检测方法特异性较强,与黄渤海其他常见钵水母种类海月水母、白色霞水母、沙海蜇均无交叉反应.利用本研究建立的海蜇LAMP检测方法对不同海域采集的成体海蜇样品和海蜇螅状幼体进行检测,结果显示,成体海蜇和海蜇幼体均呈现阳性结果,表明海蜇LAMP检测方法可以对海蜇幼体进行快速有效的检测.海蜇LAMP检测方法简单快速、灵敏且特异性强,可使其运用于沿海渔业部门海蜇资源的调查工作.     

Analysis of simple shear tests with cell pressure confinement

The high cost of offshore infrastructure provides continuous encouragement for optimisation of design practices. Development of a more rational method to interpret results from simple shear tests with cell pressure confinement can reduce costs and improve reliability of offshore infrastructure. This paper addresses a commonly overlooked issue affecting design parameter selection: specimen shape varies from right cylinder to oblique cylinder after loading along a single shearing direction. Thus, horizontal stresses are not always equal to the cell pressure and their magnitude varies throughout the specimen’s lateral surface. An analysis is proposed that accounts for changing specimen geometry and lateral surface area during shearing and for the actual effect of cell pressure during testing. The analysis also describes how the intermediate principal stress can be assessed. Test results for medium dense silica sand are interpreted following de Josselin de Jong’s alternative shearing mechanism hypothesis. Conventional interpretation methods yield conservative design parameters for this soil. Failure states develop when the intermediate principal effective stress is halfway between major and minor principal effective stresses. Typical results for the soil tested show equipment performance meets standard direct simple shear requirements for shear strain rate, vertical stress and specimen height control.     

Seismic performance of masonry walls retrofitted with steel reinforced grout

An innovative solution for the seismic protection of existing masonry structures is proposed and investigated through shake table tests on a natural scale wall assemblage. After a former test series carried out without reinforcement, the specimen was retrofitted using Steel Reinforced Grout. The strengthening system comprises horizontal strips of ultra‐high strength steel cords, externally bonded to the masonry with hydraulic lime mortar, and connectors to transversal walls, applied within the thickness of the plaster layer. In order to assess the seismic performance of the retrofitted wall, natural accelerograms were applied with increasing intensity up to failure. Test results provide a deep understanding of the effectiveness of mortar‐based composites for improving the out‐of‐plane seismic capacity of masonry walls, in comparison with traditional reinforcements with steel tie‐bars. The structural implications of the proposed solution in terms of dynamic properties and damage development under earthquake loads are also discussed.Copyright © 2015 John Wiley & Sons, Ltd.     

考虑挤土效应时楔形桩纵向振动阻抗研究

基于复刚度传递多圈层平面应变模型,研究考虑桩周土挤土效应时成层地基中楔形桩的纵向振动问题。首先根据桩周土体的纵向成层情况并考虑楔形桩的变截面特性,将桩土系统沿纵向划分为有限个微元段,对每个微元段的桩周土体建立复刚度传递多圈层平面应变模型,并通过剪切复刚度递推方法求得桩周土作用在桩身的剪切复刚度;然后将求得的剪切复刚度代入桩身纵向振动控制方程,运用Laplace变换技术和阻抗函数递推方法,推导得到考虑桩周土挤土效应时成层地基中楔形桩纵向振动时桩顶复阻抗的解析解;最后,采用参数研究方法在低频范围内分析挤土效应对桩顶复阻抗的影响及其规律。     

新型卧式土工平面应变仪研制

平面应变仪是一种开发较早但至今尚不完善的一种土工试验仪器,随着国家基础设施建设的发展,平面应变试验及试验方法在许多领域得以应用,显示出了平面应变试验在工程设计和研究中的重要性。通过回顾国内外平面应变仪的发展,提出了开发一种新型的土工平面应变三轴仪的思路,以实现更科学、更精确地进行复杂应力加载条件下土平面应变试验的研究。以此为目的,从压力室结构、加载系统及控制量测系统等方面提出了相应的改善措施,开发了一种新型的土工平面三轴仪。并通过重塑黄土的平面应变试验及改进的真三轴仪平面应变试验对比、以及平面应变试验强度参数与常规三轴试验强度参数对比分析,验证了所研制的新型卧式土工平面应变仪的合理性和研制思路的正确性。最后,通过对该平面应变仪应变试验的黄土破坏特性与改进真三轴平面应变试验的黄土破坏特征的对比分析,验证了本文所开发的平面应变仪在描述土体应变局部化问题的合理性。     

Bidirectional behavior of unreinforced masonry walls

Most of the studies related to the modeling of masonry structures have by far investigated either the in‐plane (IP) or the out‐of‐plane (OP) behavior of walls. However, seismic loads mostly impose simultaneous IP and OP demands on load‐bearing or shear masonry walls. Thus, there is a need to reconsider design equations of unreinforced masonry walls by taking into account bidirectional effects. The intent of this study is to investigate the bidirectional behavior of an unreinforced masonry wall with a typical aspect ratio under different displacement‐controlled loading directions making use of finite element analysis. For this purpose, the numerical procedure is first validated against the results of the tests on walls with different failure modes conducted by the authors. Afterward, the response of the wall systems is evaluated with increasing top displacement having different orientations. A set of 19 monotonic and three cyclic loading analyses are performed, and the results are discussed in terms of the variation of failure modes and load–displacement diagrams. Moreover, the results of wall capacity in each loading condition are compared with those of the ASCE41‐06 formulations. The results indicate that the direction of the resultant force, vectorial summation of IP and OP forces, of the wall is initially proportional to the ratio of stiffness in the IP and the OP directions. However, with the increase of damage, the resultant force direction inclines towards the wall's longitudinal direction regardless of the direction of the imposed displacement. Finally, recommendations are made for applicability of ASCE41‐06 formulations under different bidirectional loading conditions. Copyright © 2014 John Wiley & Sons, Ltd.     

利用精确定位余震资料确定2014年云南鲁甸6.5级地震的断层面参数

运用双差定位法对2014年云南鲁甸6.5级地震序列余震进行重新定位,得到565次小震的精确定位结果.根据小震丛集性原则,求解发震断层参数的数学模型,运用模拟退火全局搜索和高斯牛顿局部搜索相结合的方法对地震发震断层面参数进行反演,得到鲁甸6.5级地震断层面的走向、倾角分别为160.8°和89.1°,并对其构造环境进行了分析.     

利用断层围陷波研究昆仑山口西8.1级地震破裂面

利用横跨地表破裂带的小点距的地震测线, 对2001年11月14日昆仑山口西8.1级地震进行了断层围陷波的观测实验. 经过数字滤波和频谱分析等技术, 由地震记录图中分离出了断层围陷波. 资料处理结果表明: ① 无论是人工地震震源还是天然地震震源, 只要位于断层带内或紧靠断层带, 均能激发断层围陷波; ② 断层围陷波的能量主要集中于断层带内, 其振幅随测点与断层带距离的增加而急剧衰减; ③ 断层围陷波的优势频率与断层的宽度及断层带内介质的速度有关, 断层带越宽, 或断层带内部介质速度越低, 则观测到的断层围陷波的优势频率越低; ④ 断层围陷波存在着频散现象; ⑤ 根据昆仑山口西地震测线断层围陷波的观测结果, 可推断该处破裂面宽度为300 m左右, 远远大于地表破裂带的宽度.      

大陆岩石圈塑性流动网络的延性弱面效应

岩石圈下层的网状塑性流动 ,作为包含塑性流动网络的黏塑性流动 ,控制着大陆板块内部的构造变形和动力学过程。塑性流动网络由两组网带共轭相交而成 ,而塑性流动网带是黏塑性流动过程中因剪切局部化、黏性摩擦生热和网带介质的弱化而形成的延性弱面 (弱带 )。研究表明 ,类似于断裂和节理等脆性弱面 ,延性弱面对介质强度的影响也具有条件性 ,即当应力方向改变时 ,只有在滑移角θ不超出一定限值的条件 (θ1≤θ≤θ2 )下才可能沿原有弱面滑移 ,显示其弱化效应 ;延性弱面可以用弱化度R表示其屈服限的相对降低程度 ,弱化度与滑移角下限值之间的关系为R =sin2θ1;根据亚洲中东部地区“塑性流动 -地震”网络的最大共轭角推算 ,网带的弱化度R近似于 0 81。基于延性弱面效应的认识 ,文中就网带由剪切滑移向压性褶皱的转化、网带的继承与弃置以及应力方向的允许偏角等问题进行了探讨     

平面应变下结构性对黄土基坑稳定性的影响

鉴于以往很少考虑黄土结构性对基坑开挖的影响,针对目前普遍存在的基坑稳定性问题,开展室内平面应变条件下的真三轴试验,建立黄土结构性与强度之间关系,通过FISH语言将其引入到基坑开挖数值模拟中,分析结构性对基坑开挖的影响。研究表明,结构性参数与强度指标的变化规律与以往研究结果基本一致,表现为结构性参数与黏聚力呈双曲线关系,但参数略有不同,其受摩擦角的影响较小;结构性影响下基坑侧壁出现明显滑带,且此时连续墙的侧向位移大于不考虑其影响时的位移,坑底隆起量亦如此。研究结果对黄土基坑工程的设计和施工具有一定的指导意义。