一株H_5N_1亚型禽流感病毒株NA基因的克隆与序列分析

用RT-PCR方法从1个H5N1亚型禽流感病毒分离株A/Chicken/Guangdong/DH/1997扩增NA基因cDNA片段,将其克隆至pMD18-T载体,获得重组质粒pMD-NA,并对其核苷酸序列进行测定和分析。结果表明,该毒株的NA基因长度为1350bp,编码449个氨基酸,与其它H5N1亚型AIV分离株的核苷酸序列同源性为97.0%～99.4%,氨基酸序列同源性为97.7%～99.1%,提示禽流感病毒NA基因保守性较高。NA基因氨基酸序列的聚类分析表明该毒株与来自香港的A/Pheasant/HK/FY155/01和A/Ch/HK/FY150/01两个分离株处于同一进化枝,亲缘关系较近。     

Domal structures and high-grade metamorphism in the Higher Himalayan Crystalline, Zanskar Region, north-west Himalaya, India

ABSTRACT In the main Himalayan range in the Ladakh-Zanskar area, domal structures have been observed at structurally deeper levels in the tectonic unit of the Higher Himalayan Crystalline. Their formation occurred during a second, temperature-dominated phase (M₂) of high-grade regional metamorphism, characterized by the semipelitic paragenesis of sillimanite-K-feldspar and incipient anatexis. The doming event reveals a local system of synmetamorphic uplift superimposed on a regional system of northeast-southwest trending compression. In the main Himalayan range the development of the dominant S₂ foliation is related to deformation during the doming phase, which started early in the M₂ event. The deformation propagated continuously north-east and south-west with time. In the north-east, on the northern slopes of the main Himalayan range, this deformation is expressed by extensional shear movements of the upper tectonic levels finally leading to the late- to postmetamorphic normal fault system of the Zanskar shear zone. Towards the south-west, deformation is expressed by compressional movements, e.g. at the Main Central Thrust (MCT) in the Kishtwar window area. The observed compression and extension is inferred to relate to an increased uplift of the domal bulges of the tectonic Kishtwar window and of the whole main Himalayan range.     

Sequence stratigraphy and architecture of a late Early–Middle Aptian carbonate platform succession from the western Maestrat Basin (Iberian Chain, Spain)

The attributes of a ‘four-systems-tract’ sequence are at times difficult to identify in outcrop-scale carbonate successions. Poor exposure conditions, variable rates of sediment production, erosion and/or superposition of surfaces that are intrinsic to the nature of carbonate systems frequently conceal or remove its physical features. The late Early–Middle Aptian platform carbonates of the western Maestrat Basin (Iberian Chain, Spain) display facies heterogeneity enabling platform, platform-margin and slope geometries to be identified, and provide a case study that shows all the characteristics of a quintessential four systems tract-based sequence. Five differentiated systems tracts belonging to two distinct depositional sequences can be recognized: the Highstand Systems Tract (HST) and Forced Regressive Wedge Systems Tract (FRWST) of Depositional Sequence A; and the Lowstand Prograding Wedge Systems Tract (LPWST), Transgressive Systems Tract (TST) and subsequent return to a highstand stage of sea-level (HST) of Depositional Sequence B. An extensive carbonate platform of rudists and corals stacked in a prograding pattern marks the first HST. The FRWST is constituted by a detached, slightly cross-bedded calcarenite situated at the toe of the slope in a basinal position. The LPWST is characterized by a small carbonate platform of rudists and corals downlapping over the FRWST and onlapping landwards. The TST exhibits platform backstepping and marly sedimentation. Resumed carbonate production in shelf and slope settings characterizes the second HST. A basal surface of forced regression, a subaerial unconformity, a correlative conformity, a transgressive surface and a maximum flooding surface bound these systems tracts, and are well documented and widely mappable across the platform-to-basin transition area analyzed. Moreover, the sedimentary succession studied is made up of four types of parasequence that constitute stratigraphic units deposited within a higher-frequency sea-level cyclicity. Ten lithofacies associations form these basic accretional units. Each facies assemblage can be ascribed to an inferred depositional environment in terms of bathymetry, hydrodynamic conditions and trophic level. The architecture of the carbonate platform systems reflects a flat-topped non-rimmed depositional profile. Furthermore, these carbonate shelves are interpreted as having been formed in low hydrodynamic conditions. The long-term relative fall in sea-level occurred during the uppermost Early Aptian, which subaerially exposed the carbonate platform established during the first HST and resulted in the deposition of the FRWST, is interpreted as one of global significance. Moreover, a possible relationship between this widespread sea-level drop and glacio-eustasy seems plausible, and could be linked to the cooling event proposed in the literature for the late Early Aptian. Because of the important implications in sequence stratigraphy of this study, the sedimentary succession analyzed herein could serve as an analogue for the application of the four-systems-tract sequence stratigraphic methodology to carbonate systems.     

Stratigraphy, depositional environments and level reconstruction of the last interglacial Lake Samra in the Dead Sea basin

In this paper we describe the stratigraphy and sediments deposited in Lake Samra that occupied the Dead Sea basin between ∼ 135 and 75 ka. This information is combined with U/Th dating of primary aragonites in order to estimate a relative lake-level curve that serves as a regional paleohydrological monitor. The lake stood at an elevation of ∼ 340 m below mean sea level (MSL) during most of the last interglacial. This level is relatively higher than the average Holocene Dead Sea (∼ 400 ± 30 m below MSL). At ∼ 120 and ∼ 85 ka, Lake Samra rose to ∼ 320 m below MSL while it dropped to levels lower than ∼ 380 m below MSL at ∼ 135 and ∼ 75 ka, reflecting arid conditions in the drainage area. Lowstands are correlated with warm intervals in the Northern Hemisphere, while minor lake rises are probably related to cold episodes during MIS 5b and MIS 5d. Similar climate relationships are documented for the last glacial highstand Lake Lisan and the lowstand Holocene Dead Sea. Yet, the dominance of detrital calcites and precipitation of travertines in the Dead Sea basin during the last interglacial interval suggest intense pluvial conditions and possible contribution of southern sources of wetness to the region.     

大陆地壳深熔作用中自由水的贡献——以高喜马拉雅淡色花岗岩为例

高喜马拉雅淡色花岗岩是印度与亚洲板块碰撞过程中,地壳深熔作用的产物.尽管自由水对高喜马拉雅淡色花岗岩形成的重要性已被多次报导,但仍存在许多争议.本研究汇编了高喜马拉雅淡色花岗岩的全岩地球化学数据,证明高喜马拉雅淡色花岗岩可以由脱水熔融形成和水致熔融形成.进一步通过热力学模拟获得锆石结晶,并与锆石Ti温度计比较,限定了不...     

中东地区阿普特阶Shuaiba组碳酸盐岩沉积体系特征及模式探究

中东地区主力产油层系Shuaiba组的勘探及研究逐渐以地层岩性圈闭为主,笔者通过总结Bab盆地阿普特阶Shuaiba组沉积相类型,多油田地层格架及空间展布特征的对比,为系统恢复Shuaiba组沉积演化过程及模式提供地质依据。研究表明,阿普特阶Shuaiba组共发育开阔台地、台地边缘、台缘斜坡及盆地相4种沉积相单元和6种亚相类型,各相带单元受控于四个三级层序且沿Bab盆地周缘呈带状展布。开阔台地一侧,主要沉积了第二个三级层序对应的Lower Shuaiba地层;台地边缘往Bab台内盆地区域,则以第三和第四个三级层序对应的Upper Shuaiba地层为主。Shuaiba组生物礁、滩建造集中发育在第二个三级层序的高位域阶段,滩间海、缓坡及盆地相受控于海进和低位体系域。阿普特阶Shuaiba台地边缘生物建隆、台内浅滩在空间上相互叠置并成带展布,符合弱镶边型缓坡台地沉积模式,该类型台地具备深水陆棚性质的非常宽缓的斜坡,且缓坡往盆地一侧发育一个镶边台地边缘。     

Seismic Activity in the Himalayan Orogenic Belt and Its Related Geohazards

Himalayan orogenic belt is the highest and largest continental collision and subduction zone on the Earth. The Himalayan orogenic belt has produced frequent large earthquakes and caused several geohazards due to landslides and housing collapse, having an impact on the safety of life and property along a length of over 2500 km. Here we took three earthquake clusters as examples, which occurred at Nepal Himalaya, eastern Himalayan syntaxis and western Himalayan syntaxis, respectively. Here we calculated the earthquake locations and fault plane solutions based on the waveform data recorded by seismic stations deployed in source areas by the Institute of Tibetan Plateau Research, Chinese Academy of Sciences. We found that at the Nepal Himalayan, the Main Himalayan Thrust is the major tectonic structure for large earthquakes to occur. At the eastern Himalayan syntaxis, most earthquakes are of the reverse or strike-slip faulting. The major tectonic feature is the combination of the NE-dipping thrust with the southeastern escape of the Tibetan plateau. At the western Himalayan syntaxis, intermediate-depth earthquakes are active. These observations reveal the geometry of the deep subduction of the continental plate with steep dipping angle.     

Understanding the hydrological system dynamics of a glaciated alpine catchment in the Himalayan region using the J2000 hydrological model

This paper provides the results of hydrological modelling in a mesoscale glaciated alpine catchment of the Himalayan region. In the context of global climate change, the hydrological regime of an alpine mountain is likely to be affected, which might produce serious implications for downstream water availability. The main objective of this study was to understand the hydrological system dynamics of a glaciated catchment, the Dudh Kosi River basin, in Nepal, using the J2000 hydrological model and thereby understand how the rise in air temperature will affect the hydrological processes. The model is able to reproduce the overall hydrological dynamics quite well with an efficiency result of Nash–Sutcliffe (0.85), logarithm Nash–Sutcliffe (0.93) and coefficient of determination (0.85) for the study period. The average contribution from glacier areas to total streamflow is estimated to be 17%, and snowmelt (other than from glacier areas) accounts for another 17%. This indicates the significance of the snow and glacier runoff in the Himalayan region. The hypothetical rise in temperature scenarios at a rate of +2 and +4 °C indicated that the snowmelt process might be largely affected. An increase in snowmelt volume is noted during the premonsoon period, whereas the contribution during the monsoon season is significantly decreased. This occurs mainly because the rise in temperature will shift the snowline up to areas of higher altitude and thereby reduce the snow storage capacity of the basin. This indicates that the region is particularly vulnerable to global climate change and the associated risk of decreasing water availability to downstream areas. Under the assumed warming scenarios, it is likely that in the future, the river might shift from a ‘melt‐dominated river’ to a ‘rain‐dominated river’. The J2000 model should be considered a promising tool to better understand the hydrological dynamics in alpine mountain catchments of the Himalayan region. This understanding will be quite useful for further analysis of ‘what‐if scenarios’ in the context of global climate and land‐use changes and ultimately for sustainable Integrated Water Resources Management in the Himalayan region. Copyright © 2012 John Wiley & Sons, Ltd.     

粤港澳大湾区生态系统服务价值的时空演化及其影响机制

生态系统服务是生态要素、结构、功能和产品造福于人类社会的媒介和通道。根据生态系统服务价值（ESV）及其影响机制调节人类社会对生态系统的利用强度和保护方式,对于实现人地和谐的可持续发展目标具有重要意义。本文以粤港澳大湾区作为研究对象,依托遥感数据修订ESV核算结果,更加准确地揭示2000—2015年粤港澳大湾区ESV的时空演变特征,并引入面板分位数回归深入探索生态系统服务影响因素的分段效应,增加对生态系统服务影响机制的认识。结果显示：① 2000—2015年粤港澳大湾区ESV减速下降,下降区域主要分布在粤港澳大湾区中部和珠江入海口两岸等城市快速扩张区。② 在城市交界处,土地利用变化会带来更为强烈的ESV变化。③ 在ESV水平不同的区域,影响因素具有不同的影响效果。用地完整度仅在ESV的低值区可以促进生态系统服务能力提升。气温对生态系统服务能力的正向作用随ESV的提高而增强,经济密度对生态系统服务能力的负向作用随ESV的提高而减弱,二者均会导致ESV高值区与低值区间的差距增大,形成“自然马太效应”。     

Oil and gas development exposure and conservation scenarios for Greater sage-grouse: Combining spatially explicit modeling with GIS visualization provides critical information for management decisions

During the last quarter-century, global demand for energy has increased by more than 60%, and a similar increase is anticipated to occur by 2030 (Raymond, Deming, & Nichols, 2007). In the U.S., oil and gas development is projected to continue across western states within sage-grouse habitat. Greater sage-grouse, recently a candidate species for protection under the Endangered Species Act (ESA), have well documented negative responses to oil and gas disturbance. In this study, we create spatially-explicit oil and gas future development scenarios, baseline and high, and link them to sage-grouse population and habitat maps to quantify future exposure risk within Western Association of Fish and Wildlife Agencies (WAFWA) sage-grouse management zones (MZ) I and II. We then analyze recent land use decisions from the Bureau of Land Management (BLM) along with enacted policy from the State of Wyoming to estimate how these management actions might minimize the exposure risk of sage-grouse to oil and gas development into the future. Our results show that BLM and Wyoming conservation plans could reduce the exposure of sage-grouse to oil and gas development from 15-27% to 11–17% (31–37% reduction) in MZ I and from 15-27% to 5–9% (64–68% reduction) in MZ II. Our estimates of exposure to future oil and gas development, and conservation measures designed to ameliorate those threats, represent the upper and lower extents of potential impacts within scenarios. Our work demonstrates how spatial modeling and GIS visualization can be used by managers to assess likely outcomes of conservation decisions.