石斑鱼虹彩病毒SGIV在宿主细胞内依赖微管运动的行为特征

病毒是非常微小的简单生物, 不能独立生存, 必须借助宿主细胞完成自身的繁衍。病毒侵染进入细胞后, 通常借助于微管通过黏稠的细胞质运动到特定的复制位点。然而, 有关病毒依赖微管运动行为的精细动态研究还比较少。石斑鱼虹彩病毒(Singapore grouper iridovirus, SGIV)为虹彩病毒科蛙病毒属的一个新种, 是海水养殖鱼类的重要病毒性病原, 对海水养殖业造成重大经济损失。利用单粒子示踪技术实时追踪了SGIV病毒粒子沿微管运动的行为, 观察到SGIV在细胞边缘至微管中心之间的双向运动, 最高瞬时速度约0.2μm·s^-1, 均表现为主动运输。病毒粒子运动至微管交叉位置会减速迂回, 而后或受限于此, 平均运动速率约0.008μm·s^-1, 或通过交叉处继续快速运动, 最高瞬时速度为0.2μm·s^-1。同时, SGIV感染会影响微管的形态结构, 随着SGIV感染, 微管逐渐围绕细胞核和病毒加工厂形成环状结构。研究结果初步揭示了SGIV病毒和细胞微管之间相互作用的复杂过程, 丰富了我们对虹彩病毒胞内生命活动的认识, 有助于深入地理解海水鱼类虹彩病毒感染致病机理。     

南海大气低层风场的气候特征分析

分析１９８０－１９８７年欧洲中长期预报中心（ＥＣＭＷＦ）每日风场、温度场格点资料及同期南海区域测站和船舶资料，讨论南海低层风场的气候特征。南海为典型的季风活动区，冬季盛行东北季风，夏季盛行西南季风。流场的季节转换表现为季风系统的交替，相应不同的季风系统，南北温度梯度有一逆转过程，南海北部是温度梯度大且发生明显逆转的海区。１２°Ｎ以南海区气温全年变化极小，整个海区大气温度的季节变化不如流场变化快和显著。     

人工养虾池生态系统结构特点及其控制对策

为养殖对虾而修筑的大大小小的养虾池都是一相对独立的生态系统。在池内生态条件适于对虾正常要求的情况下，对虾可以在其中生存、生长。如果生态条件逐步恶化，非但影响对虾的正常生活、生长，严重时甚至导致全部对虾死亡。粗放式的养殖，其影响也相对小些，高密度养殖情况下潜在的危险较大，对养殖池的环境状况要特别注意。ｌ人工养虾生态系统的基本特点１·１人工养殖池是一个结构简单的生态系统。在池内除放养的对虾（有时混养贝类等）外自然存在的生物通常是浮游植物和浮游动物，食物链大体上是二个层次（对虾靠投饵喂养例外）。养虾池…     

黄河三角洲潮滩发育时空谱系

研究黄河三角洲潮滩发育的时空话系指出：在时间上，黄河三角洲潮滩系由不同时期河口滩发育而成，各段潮滩发育时间因素的差异在比较形态学上有清晰反映，在空间上，并存着处于不同发育阶段上的四种类型（阶段）。由此构成了黄河三角洲前沿所特有的湖滩形态时空体系。     

Tectonics of a fast spreading center: A Deep-Tow and sea beam survey on the East Pacific rise at 19°30′ S

Sea Beam and Deep-Tow were used in a tectonic investigation of the fast-spreading (151 mm yr^-1) East Pacific Rise (EPR) at 19°30 S. Detailed surveys were conducted at the EPR axis and at the Brunhes/Matuyama magnetic reversal boundary, while four long traverses (the longest 96 km) surveyed the rise flanks. Faulting accounts for the vast majority of the relief. Both inward and outward facing fault scarps appear in almost equal numbers, and they form the horsts and grabens which compose the abyssal hills. This mechanism for abyssal hill formation differs from that observed at slow and intermediate spreading rates where abyssal hills are formed by back-tilted inward facing normal faults or by volcanic bow-forms. At 19°30 S, systematic back tilting of fault blocks is not observed, and volcanic constructional relief is a short wavelength signal (less than a few hundred meters) superimposed upon the dominant faulted structure (wavelength 2–8 km). Active faulting is confined to within approximately 5–8 km of the rise axis. In terms of frequency, more faulting occurs at fast spreading rates than at slow. The half extension rate due to faulting is 4.1 mm yr^-1 at 19°30 S versus 1.6 mm yr^-1 in the FAMOUS area on the Mid-Atlantic Ridge (MAR). Both spreading and horizontal extension are asymmetric at 19°30 S, and both are greater on the east flank of the rise axis. The fault density observed at 19°30 S is not constant, and zones with very high fault density follow zones with very little faulting. Three mechanisms are proposed which might account for these observations. In the first, faults are buried episodically by massive eruptions which flow more than 5–8 km from the spreading axis, beyond the outer boundary of the active fault zone. This is the least favored mechanism as there is no evidence that lavas which flow that far off axis are sufficiently thick to bury 50–150 m high fault scarps. In the second mechanism, the rate of faulting is reduced during major episodes of volcanism due to changes in the near axis thermal structure associated with swelling of the axial magma chamber. Thus the variation in fault spacing is caused by alternate episodes of faulting and volcanism. In the third mechanism, the rate of faulting may be constant (down to a time scale of decades), but the locus of faulting shifts relative to the axis. A master fault forms near the axis and takes up most of the strain release until the fault or fault set is transported into lithosphere which is sufficiently thick so that the faults become locked. At this point, the locus of faulting shifts to the thinnest, weakest lithosphere near the axis, and the cycle repeats.     

Does subterranean flow initiate mud banks off the southwest coast of India?

Coastal waters off the southwest coast of India draw special attention because of the occurrence of mud banks at certain locations during southwest monsoon period. The present study puts forward a hypothesis of a subterranean flow, which could be a plausible mechanism to initiate the mud banks. The subterranean flow is believed to be coupled with activated trending faults and originate from the adjacent watershed (Vembanad Lake) separated from the sea by a narrow strip of land where submerged porous lime shell beds are present. When the lake water injection occurs through the fault, the mud/clay gets excited by its thixotrophic properties (as the overlying water looses its electrolyte) and transforms into a flowing fluid. The lowering of salinity due to the introduction of fresh water keeps the mud suspension in the water column for longer duration, leading to the formation of mud banks. The idea of subterranean flow through lime shell beds initiating formation of mud banks may apply globally to any coastal regions hugged by wetlands and of similar geological conditions.     

褐藻酸降解菌的产酶条件研究

以海带病烂处分离到的别单胞菌属（Alkteromonas)的褐藻酸降解菌菌株A1为研究对象，在该菌的产酶条件进行了研究。结果表明，产酶的最适温度20℃，褐藻酸钠浓度（质量分数）0．5％－0．6％，pH7.5，盐度15，氮源为（NH4)2SO4，培养时间72h。     

Biochemical Mechanism of the Eutrophication and Its Prevention—the Deep Treatment of Waste Water and Its Denitrification and Dephosphorization

Biochemical mechanism of forming the red tide is discussed in this paper.The existence of a large number of nitrates and phosphates in the eutrophic water is the prerequisite of explosive increase of algae and the forming of red tide.Reduction of eutrophication is an important approach to preventing the red tide.The method of deep treatment of the waste water and its denitrification and dephosphorization are introduced,and a new opinion on the red tide formation and fundamental prevention is put forward.     

渐新世/中新世分界的地层学事件

介绍渐新世/中新世分界面(即古近纪/新近纪分界面)附近的浮游有孔虫和钙质超微化石事件及其新年龄。2004年国际地层年代表确定该分界面位于第58长偏心率周期弱振幅处,古地磁事件C6Cn.2n底面,经天文调谐后的年龄为23.03 Ma。发生在该界面附近的生物地层学事件主要是浮游有孔虫Paragloborotalia kugleri的始现面(22.96 Ma)和钙质超微化石Sphenolithus delphix的末现面(23.11 Ma)。在南海北部东沙群岛附近的大洋钻探ODP1148站,渐新世/中新世界面以崩塌沉积物为特征,有明显沉积间断,标志南海当时有较大范围的构造运动。     

褐藻胶裂解酶产生菌Vibro sp.QY102的发酵条件优化

对海洋来源的Vibro sp.QY102的产褐藻胶裂解酶的发酵条件进行研究。结果表明,该菌株最适液体培养基成分为(w/v):0.5%褐藻酸钠;0.4%蛋白胨;0.3%KH2PO4;0.7%K2HPO4.3H2O;2%NaCl;0.01%MgSO4.7H2O,pH=6.0。按3%的接种量接入培养基,30℃150 r/min振荡培养120 h,产酶达到10.2 U/mL,为优化前的4.5倍。Mg2 是该菌株产酶所必需的,这在其他褐藻胶裂解酶生产菌株中未见报道。该菌株产酶发酵条件的研究,为褐藻胶裂解酶的大规模制备及应用奠定了基础。