Improved Method for Calculating Anthropogenic CO2 in the Upper Layer of the North Pacific Subtropical Gyre along 175°E

Seawater samples were collected in the North Pacific along 175°E during a cruise of the Northwest Pacific Carbon Cycle Study (NOPACCS) program in 1994. Many properties related to the carbonate system were analyzed. By using well-known ratios to correct for chemical changes in seawater, the CO₂ concentration at a given depth was back calculated to its initial concentration at the time when the water left the surface in winter. We estimated sea-surface CO₂ and titration alkalinity (TA) in present-day winter, from which we evaluated the degree of air-sea CO₂ disequilibrium in winter was. Using a correction factor for air-sea CO₂ disequilibrium in winter, we reconstructed sea-surface CO₂ in pre-industrial times. The difference between the back-calculated initial CO₂ and sea-surface CO₂ in pre-industrial times should correspond to anthropgenic CO₂ input. Although the mixing of different water masses may cause systematic error in the calculation, we found that the nonlinear effect induced by the mixing of different water masses was negligible in the upper layer of the North Pacific subtropical gyre along 175°E. The results of our improved method of assessing the distribution of anthropogenic CO₂ in that region show marked differences from those obtained using the previous back-calculation method.     

黄精复方治疗气阴两虚型2型糖尿病32例临床观察

目的：探讨黄精复方治疗气阴两虚型2型糖尿病的临床疗效。方法：将气阴两虚型2型糖尿病患者64 例随机分为治疗组和对照组,每组各32 例。所有患者均采取饮食、运动等生活方式干预,对照组口服二甲双胍片治疗,治疗组在对照组基础上加用黄精复方治疗,疗程均为12周。观察2组综合疗效及治疗前后中医证候积分、体质量指数（BMI）、空腹血糖（FBG）、餐后2 h血糖（2hPG）、糖化血红蛋白（HbA1c）、空腹胰岛素（FINS）、胰岛素抵抗指数（HOMA-IR）、总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）的变化,记录2组不良反应。结果：总有效率治疗组为87.50%（28/32）,高于对照组的59.37%（19/32）,差异有统计学意义（P＜0.05）。2组中医证候积分、BMI、FBG、2hPG、HbA1c、FINS、HOMA-IR、TC、TG、LDL-C、HDL-C治疗前后组内比较及治疗后组间比较,差异均有统计学意义（均P＜0.05）。结论：黄精复方治疗气阴两虚型2型糖尿病可以缓解患者的中医证候,降低血糖、血脂,改善胰岛功能,从而提高临床疗效。     

基于新型高频声学多普勒流速剖面仪的河口近底部边界层观测初探

河口物质输运、能量交换与底边界层内的水动力过程密切相关,底边界层参数(如切应力、拖曳系数)的确定至关重要。挪威Nortek公司生产的新型声学多普勒流速剖面仪AD2CP相比传统ADCP具有高频、低噪的优点,可用于高频(16Hz)流速剖面观测,而被广泛应用于底边界层观测的ADV只能测量单点高频流速。本文采用AD2CP在长江口南槽最大浑浊带区域进行座底式观测,并与同步近底部三脚架上ADV的观测结果进行对比。结果表明,使用AD2CP测得的近底部平均流速与ADV的测量结果吻合良好;使用惯性耗散法计算了底切应力,基于ADV的单点高频流速数据计算结果为2.16×10~(-2)～5.69×10~(-1)N/m~2,基于AD2CP的结果为2.09×10~(-2)～4.26×10~(-1)N/m~2,二者范围大致相当。在此基础上,基于AD2CP数据计算出摩阻流速为4.55×10~(-3)～2.06×10~(-2)m/s、底拖曳系数范围为1.84×10~(-4)～2.49×10~(-3),与ADV的计算结果基本一致。此外,由于AD2CP可以获得高频的流速剖面数据,优于单点ADV,具备观测近底部边界层参数和边界层内湍流剖面的潜力。     

九孔鲍BMP-2基因cDNA克隆及表达分析

为探究BMP-2基因在九孔鲍(Haliotisdiversicolorsupertexta)中的功能,采用cDNA末端快速扩增(RACE)技术从九孔鲍外套膜中获得了BMP-2基因cDNA全长,并用实时荧光定量PCR(qRT-PCR)检测了BMP-2基因在各组织和发育时期的表达水平。结果表明:九孔鲍BMP-2基因cDNA全长2572bp,其中5′非编码区(5′UTR)123bp, 3′非编码区(3′UTR)1150bp,开放阅读框(ORF)为1299 bp,编码432个氨基酸,其分子质量为48.59ku,理论等电点(pI)为9.84;具有N端信号肽(1-39 aa)、TGF-β前肽区域(63-294 aa)和TGF-β成熟肽区域(331-432 aa),以及蛋白酶水解位点RLRR (272-275 aa)和7个保守的半胱氨酸残基,符合TGF-β超家族蛋白典型结构特征。系统进化树结果显示九孔鲍BMP-2基因和耳鲍聚为一支。qRT-PCR结果表明BMP-2基因在九孔鲍的6个组织中均有表达,在足、右侧壳肌、外套膜及肝脏中显著高表达;在检测的7个发育时期均表达,其中在受精卵、4细胞、8细胞、原肠胚、稚鲍时期表达量显著高于卵、幼鲍时期。研究表明BMP-2基因可能在九孔鲍贝壳形成中具有重要作用。     

基于GNSS浮标和验潮资料的HY-2A卫星高度计绝对定标

为探测我国HY-2A卫星高度计海面高测量绝对偏差及其在轨运行状态,本文利用GNSS浮标星下点同步测量和验潮资料海面高传递方法在山东千里岩和珠海担杆岛海域开展定标研究。为验证GNSS浮标定标方法的准确性,还对国外卫星Jason-2和Saral进行了定标实验。实验表明GNSS浮标绝对海面高测量精度达2 cm,对Jason-2和Saral高度计多个周期定标得到的海面高偏差均值分别为5.7 cm和-2.3 cm,与国际专门定标场的结果符合较好。2014年9月和2015年5月HY-2A卫星高度计浮标定标结果分别是-65 cm和-91 cm,因两次结果差异显著,故又利用千里岩验潮站资料对HY-2A卫星高度计第56至73周期进行了定标分析,结果证明HY-2A卫星海面高存在约-51 cm/a的漂移,置信度为95%的回归分析表明浮标和验潮定标结果符合。本文研究结果表明在我国尚无专门定标场的情况下,可利用GNSS浮标对我国高度计实施灵活、精准的在轨绝对定标,在有高度计轨迹经过验潮站的情况下可使用验潮资料结合精密大地水准面模型进行绝对定标。     

基于NS2的TCPW性能改进算法研究

TCP Westwood(TCPW)和Vegas都是基于测量的TCP New-Reno增强算法。TCPW针对ACK流直接估算可用带宽,在慢启动和线性递增部分依然采用传统Reno的盲目递增的机制,导致发送端较为频繁的重传。对这几种算法原理进行分析阐述,并提出1种改进方法,更精确的测量估计带宽,通过网络仿真工具NS2进行验证,明显去除TCP全局同步现象,增强网络性能。     

Microbial photosynthesis in coral reef sediments (Heron Reef, Australia)

We investigated microphytobenthic photosynthesis at four stations in the coral reef sediments at Heron Reef, Australia. The microphytobenthos was dominated by diatoms, dinoflagellates and cyanobacteria, as indicated by biomarker pigment analysis. Conspicuous algae firmly attached to the sand grains (ca. 100 μm in diameter, surrounded by a hard transparent wall) were rich in peridinin, a marker pigment for dinoflagellates, but also showed a high diversity based on cyanobacterial 16S rDNA gene sequence analysis. Specimens of these algae that were buried below the photic zone exhibited an unexpected stimulation of respiration by light, resulting in an increase of local oxygen concentrations upon darkening. Net photosynthesis of the sediments varied between 1.9 and 8.5 mmol O₂ m⁻² h⁻¹ and was strongly correlated with Chl a content, which lay between 31 and 84 mg m⁻². An estimate based on our spatially limited dataset indicates that the microphytobenthic production for the entire reef is in the order of magnitude of the production estimated for corals. Photosynthesis stimulated calcification at all investigated sites (0.2–1.0 mmol Ca²⁺ m⁻² h⁻¹). The sediments of at least three stations were net calcifying. Sedimentary N₂-fixation rates (measured by acetylene reduction assays at two sites) ranged between 0.9 to 3.9 mmol N₂ m⁻² h⁻¹ and were highest in the light, indicating the importance of heterocystous cyanobacteria. In coral fingers no N₂-fixation was measurable, which stresses the importance of the sediment compartment for reef nitrogen cycling.     

Characterization of tar-like material and producible oil in Misoa C2 and C3 reservoir sands of the Area 2 South (Ceuta Field,Venezuela)

Here we characterized asphaltic oil occurrences in the Misoa C2 and C3 reservoirs within Area 2 South of the Ceuta Field (Lake Maracaibo Basin, NW Venezuela). To perform the geochemical and petrological characterization of the C2 and C3 sub-units, which cover an interval in which tar-like material was detected, we analyzed core (18) and cutting (123) samples using the Rock-Eval 6 technique in reservoir mode. Samples were studied under fluorescence light and scanning electron microscopy (SEM), showing the presence of two charges of oils and the relationships within silica cements and clay minerals. To evaluate vertical inter-reservoir communication and lateral intra-reservoir continuity, we also studied 12 oil samples from wells in Area 2 South. Biomarkers and isotopic signatures were examined using gas chromatography-mass spectrometry (GC-MS) and isotope ratio mass spectrometry (IRMS). The results suggest that oil and core extracts belongs to a single family derived from two pulses of hydrocarbon generation from the La Luna source rock, which was deposited in a marine reducing environment. The oil samples are a mixture of a paleobiodegraded oil charge and a later fresh, more mature oil recharge. The geochemical analyses also evidence that the formation of heavy oil tar zones can be related to biodegradation and in-reservoir oil mixing, though other formation mechanisms for the tar are also feasible. Finally, the GC fingerprints of the oils are indicative of lateral reservoir continuities across the F-1 and F-2 faults and vertical communication between the Misoa C2 and C3 sub-units in Area 2 South, suggesting that tar occurrences do not act as permeability barriers within the two reservoirs studied.     

GC-MS联合二维核磁对文蛤多糖Fr.2A组分结构的解析

采用GC-MS联合二维核磁的方法,对文蛤均多糖结构进行解析。运用双酶辅助水提的方法,提取文蛤粗多糖(MMPX),分离纯化得到文蛤均多糖,并通过气质联用技术(GC-MS)和核磁共振波谱(1D,2D NMR),对文蛤均多糖的结构进行了分析。结果表明,从文蛤中提取得到文蛤粗多糖(MMPX),经分离纯化得到高纯度文蛤均多糖Fr.2A,其单糖组成为葡萄糖(Glc)。GC-MS分析发现,Fr.2A存在三种残基单元1,4-Glcp、T-Glcp和1,4,6-Glcp,摩尔比为5.58︰1.26︰1.0;核磁共振波谱分析表明,Fr.2A是以α-(1→4)与少量α-(1→6)连接为主链,并存在少量的α-(1→6)和β-(1→4)支链的新型水溶性D型吡喃葡聚糖。