The Development of the Chagas' Online Data Entry System (CODES-GIS)

This article describes a web‐based data entry and GIS‐driven mapping system designed for an ethnographic and entomological survey of Chagas’ disease, an emerging zoonotic disease, and Triatoma dimidiata, a primary vector, in the Los Tuxtlas region of Veracruz, Mexico. To better understand this disease in the region, a collaborative, multi‐disciplinary study was initiated to conduct a spatial investigation of T. dimidiata and a community‐by‐community survey of local perceptions of the disease. In order to facilitate such a collaborative effort the CODES‐GIS was developed. This system allows for (near) real‐time mapping, analyses, disease reporting, and results sharing. CODES‐GIS provides a framework for a research team working in a remote area with limited technology, software, or GIS expertise to benefit from (near) real‐time spatial analyses performed at collaborating institutions. The system is bi‐directional, where field personnel can upload data to the system for field‐based map production. Likewise, laboratory personnel can upload diagnostics data for viewing by field personnel. In this way, the system provides a virtual link between the field and the laboratory to increase the speed at which results are returned to the local community. The CODES‐GIS is described along with a selection of study results.     

东方百合(Lilium)杂交品种索邦病程相关蛋白(PR1)基因的克隆与原核表达

病程相关蛋白（PR）广泛参与植物对生物和非生物胁迫的应答。采用cDNA末端快速克隆技术,从东方百合（Lilium）杂交品种索邦中克隆了PR1基因,并命名为LhSorPR1。LhSorPR1基因cDNA全长870 bp,5’非翻译区和3’非翻译区的长度分别为47 bp和274 bp。该基因的开放阅读框为549 bp,编码182个氨基酸。成熟的LhSorPR1蛋白（不含信号肽）分子量为18.19 kDa,等电点为5.69。采用同源建模方式构建的LhSorPR1蛋白三维结构与其模板番茄PR1蛋白p14a具有较高的相似性,说明百合PR1蛋白在结构和功能上与其他植物中的PR1蛋白具有较强的保守性。构建pCold Ⅱ-LhSorPR1原核表达,经IPTG诱导后,目的蛋白在大肠杆菌中以包涵体形式成功表达。使用免疫印迹方法对表达的LhSorPR1重组蛋白进行了验证,并对带有His标签的重组蛋白成功进行了纯化。     

近10年我国医疗气象学研究现状及其展望

综述了近10年来国内有关医疗气象的研究,特别是介绍了前人在常见病（循环系统、消化系统和呼吸系统疾病）、传染病以及各种生物性病原（疾病媒介物、寄生虫、真菌、细菌等）与气象条件关系研究,未来气候变化对人体健康的可能影响以及气象部门在医疗气象方面的预报和服务,并对今后医疗气象的研究方向进行了展望。指出应进一步加强医疗与气象两个学科的全国性协作研究。研究各种气象因素对人体生理、病理及精神状态的影响和气候变化对人类健康的影响,加强气象灾害与疾病的关系及灾后防御措施的研究。     

对虾细胞培养的研究现状、存在问题及建立细胞系策略

近年来,对虾病毒病的爆发,使我国养殖对虾的年产量大幅度下降,造成了巨大的经济损失。就对虾细胞培养研究现状、应用前景和存在的关键问题等进行了综述和讨论。     

磁共振弥散张量成像在早期帕金森病诊断中的应用研究

目的:探讨磁共振弥散张量成像（DTI）在早期帕金森病（PD）诊断中的价值。方法:选取早期PD患者18例、正常志愿者18例,同时行头部MRI平扫及DTI检查,观察两组双侧丘脑、尾状核头、黑质、壳核及苍白球的FA值、ADC值。结果:两组间双侧丘脑、尾状核头及黑质的FA值比较,早期PD组较对照组减低,差异有统计学意义（P<0.05）。两组间壳核及苍白球的FA值比较差异无统计学意义（P>0.05）。两组间各感兴趣区（ROI）的ADC值差异无统计学意义。结论:丘脑、尾状核头及黑质的FA值有助于PD的早期诊断,DTI可为PD的早期诊断提供有价值的线索。      

16S rRNA Gene Phylogenesis of Culturable Predominant Bacteria from Diseased Apostichopus japonicus(Holothuroidea,Echinodermata)

Cultured Apostichopusjaponicus in China suffers from a kind of skin ulceration disease that has caused severe economic loss in recent years. The disease, pathogens of which are supposed to be bacteria by most researchers, is highly infectious and can often cause all individuals in the same culture pool to die in a very short time. The 16S rRNA gene phylogenesis of the culturable bacteria from the lesions of diseased individuals was conducted to study the biodiversity of the bacterial communities in the lesions and to identify probable pathogen(s) associated with this kind of disease. S. japonica samples were selected from a hatchery located in the eastern part of Qingdao, China. Bacterial universal primers GM5F and DS907R were used to amplify the 16S rRNA gene of bacteria colonies, and touchdown PCR was performed to amplify the target sequences. The results suggest that γ- proteobacteria (Alteromonadales and Vibrionales) of CFB group, many strains of which have been also determined as pathogens in other marine species, are the predominant bacterial genera of the diseased Apostichopusjaponicus individuals.     

16S rRNA gene phylogenesis of culturable predominant bacteria from diseased Apostichopus japonicus (Holothuroidea, Echinodermata)

Cultured Apostichopus japonicus in China suffers from a kind of skin ulceration disease that has caused severe economic loss in recent years. The disease, pathogens of which are supposed to be bacteria by most researchers, is highly infectious and can often cause all individuals in the same culture pool to die in a very short time. The 16S rRNA gene phylogenesis of the culturable bacteria from the lesions of diseased individuals was conducted to study the biodiversity of the bacterial communities in the lesions and to identify probable pathogen(s) associated with this kind of disease. S. japonica samples were selected from a hatchery located in the eastern part of Qingdao, China. Bacterial universal primers GM5F and DS907R were used to amplify the 16S rRNA gene of bacteria colonies, and touchdown PCR was performed to amplify the target sequences. The results suggest that γ- proteobacteria (Alteromonadales and Vibrionales) of CFB group, many strains of which have been also determined as pathogens in other marine species, are the predominant bacterial genera of the diseased Apostichopus japonicus individuals.     

GIS技术在动物疫病防控中的应用

数字畜牧是动物卫生领域的重要发展趋势。本文着重讨论GIS技术在动物疫病防控中的应用,并以辽宁省畜禽分布定位及重大动物疫病防控调度指挥系统的建设为实例提出基于SuperMap GIS平台构建动物疫病防控系统的解决方案,从而为我国畜牧业信息化建设提供参考。     

"红肉病"文蛤(Meretrix meretrix Linnaeus)中发现的3种病毒样颗粒

本文报道了在患“红肉病”文蛤组织中发现的 3种病毒样颗粒。 1种主要存在于鳃上皮下的结缔组织中 ,呈球形或椭球形 ,大小 (5 0～ 6 0 ) nm× (5 0～ 110 ) nm,无囊膜包裹。另 1种为球形 ,直径 16 0～ 180 nm,有囊膜 ,存在于外套膜上皮细胞及结缔组织细胞质中。第 3种存在于消化盲囊上皮细胞质内 ,为无囊膜包裹的球形病毒样颗粒 ,直径为 5 0～ 80 nm。     

海水养殖斜带石斑鱼溃疡病病原菌(溶藻弧菌)的初步研究

从患溃疡病的斜带石斑鱼肝脏分离到 1株细菌EpGS0 2 10 0 1,人工感染试验结果证明该菌是斜带石斑鱼的病原菌 ,对斜带石斑鱼的半致死剂量为 2 .0× 10 5CFU·g-1。对EpGS0 2 10 0 1进行了生理生化特性指标测定、API细菌鉴定系统和 16SrRNA基因序列比较鉴定。结果表明 ,该菌为溶藻弧菌。药敏试验结果表明 :头孢曲松、环丙沙星、依诺沙星、氧氟沙星、复方新诺明及阿米卡星对该菌有明显的抑制作用。