黄斑篮子鱼去毒相关基因的克隆与肝脏组成型表达分析

从基因水平探讨海洋鱼类对海洋藻毒素的去毒分子机理。采用RT-PCR法成功克隆了黄斑篮子鱼Siganus oramin肝脏I时相代谢酶细胞色素P450 1A(CYP1A)、II时相代谢酶alpha型谷胱甘肽S-转移酶(GSTA)和rho型谷胱甘肽S-转移酶(GSTR)、热休克蛋白70 (HSP70)、alpha 1型钠钾ATP酶(ATP1A1)及β-肌动蛋白(beta-actin, ACT)基因cDNA核心序列,序列分别长879 bp、582 bp、588 bp、660 bp、749 bp和554 bp。序列同源性分析发现,属鲈形目的黄斑篮子鱼CYP1A、GSTA和GSTR与同属鲈形目的牙鲆Paralichthys olivaceus、欧洲鲽Pleuronectes platessa、真鲷Pagrus major、鲤形目的斑马鱼Brachydanio rerio 相应氨基酸序列同源性较高,CYP1A和GSTA与非洲爪蟾(两栖类)、鸡(鸟类)、小鼠、大鼠和人(哺乳类)相应氨基酸序列同源性低,这可能与鱼类I、II时相去毒酶基因承担水环境毒素去毒代谢的特殊功能有关;而HSP70、ATP1A和β-肌动蛋白在鱼类、两栖类、鸟类、哺乳类中均有较高的同源性,这可能与这些基因在机体中承担的最基本的生命功能相关。应用半定量RT-PCR的方法,以β-肌动蛋白作为外参照,在指数期增长范围内分别得到了CYP1A、GSTA、GSTR、HSP70和ATP1A1 mRNA与β-肌动蛋白mRNA (%)的比值,确定黄斑篮子鱼肝脏去毒相关基因的组成型表达水平。其中,黄斑篮子鱼肝脏CYP1A、GSTA和GSTR基因组成型表达相对较高,HSP70和ATP1A1基因组成型表达相对较低,这可能与不同基因在黄斑篮子鱼海洋藻毒素去毒分子机理中承担的作用相关,为海洋藻毒素在海洋鱼类中的积聚及代谢去毒分子机制的研究提供了相关数据。     

First six months quality assessment of HY-2A SCAT wind products using in situ measurements

The first Chinese microwave ocean environment satellite HY-2A, carrying a Ku-band scatteromenter （SCAT）, was successfully launched in August 2011. The first quality assessment of HY-2A SCAT wind products is presented through the comparison of the first 6 months operationally released SCAT products with in situ data. The in situ winds from the National Data Buoy Center （NDBC） buoys, R/V Polarstern, Aurora Australis, Roger Revelle and PY30-1 oil platform, were converted to the 10 m equivalent neutral winds. The temporal and spatial differences between the HY-2A SCAT and the in situ observations were limited to less than 5 min and 12.5 km. For HY-2A SCAT wind speed products, the comparison and analysis using the NDBC buoys yield a bias of-0.49 m/s, a root mean square error （RMSE） of 1.3 m/s and an increase negative bias with increasing wind speed observation above 3 m/s. Although less accurate of HY-2A SCAT wind direction at low winds, the RMSE of 19.19° with a bias of 0.92° is found for wind speeds higher than 3 m/s. These results are found consistent with those from R/Vs and oil platform comparisons. Moreover, the NDBC buoy comparison results also suggest that the accuracy of HY-2A SCAT winds is consistent over the first half year of 2012. The encouraging assessment results over the first 6 months show that wind products from HY-2A SCAT will be useful for scientific community.     

三疣梭子蟹HMGR基因的克隆及其在蜕皮中的表达分析

为了研究3-羟基-3-甲基戊二酰辅酶A还原酶(3-hydroxy-3-methyl glutaryl coenzyme A reductase,HMGR)在甲壳动物蜕皮调控中的作用,采用RT-PCR和c DNA末端快速扩增技术(RACE),克隆得到三疣梭子蟹(Portunus trituberculatus)HMGR基因的c DNA序列(Gen Bank登录号:KF280756)。该序列全长2575bp,包括一个53bp的5′端非编码区,一个686bp的3′端非编码区和一个长度为1836bp的开放阅读框,编码611个氨基酸。该氨基酸序列与已公布的美洲海鳌虾HMGR氨基酸序列相比一致性达65%,具有Ⅰ型HMGR保守催化区域、两个HMG-Co A结合基序和两个NADP(H)结合基序。采用实时荧光定量PCR(q RT-PCR)技术,分析三疣梭子蟹HMGR基因的组织差异表达及在蜕皮周期中的表达水平变化,结果表明HMGR基因在三疣梭子蟹大颚器(MO)中的表达量最高,在其它组织中表达量均极低;在三疣梭子蟹蜕皮周期中,大颚器中HMGR基因的表达量自A期至D0亚期升至最高,然后下降,至D4亚期最低。验证了大颚器是三疣梭子蟹合成甲基法尼酯的唯一器官,表明HMGR在三疣梭子蟹蜕皮调控中起着重要作用。     

Food web structure of two Mediterranean lagoons under varying degree of eutrophication

The food web structure and functioning of two north-western Mediterranean lagoons exhibiting contrasting degrees of eutrophication and marine influences were compared through δ¹³C and δ¹⁵N analysis of major potential food sources and consumers. The Lapalme Lagoon is well preserved and has kept a natural and temporary connection with the open sea. Conversely, the Canet Lagoon is heavily eutrophicated and its water exchange with the open sea has been artificially reduced. In Lapalme, all potential food sources and consumers exhibited δ¹⁵N values indicative of pristine coastal areas. Suspended particulate organic matter (POM) and sediment organic matter (SOM) pools seemed to constitute the main food sources of most primary consumers. Both primary producers and all consumers were much more ¹⁵N-enriched (by  10‰) and more ¹³C-depleted in Canet than in Lapalme. This reflected: (1) the assimilation of important amounts of anthropogenic nitrogen in the food web, and (2) a marked and uniform influence of ¹³C-depleted allochtonous sources of carbon. Based on the mean δ¹⁵N of primary consumers, we found rather similar food web lengths in both lagoons with top consumers at trophic levels 3.6 and 4.0 in Canet and Lapalme, respectively. However, the eutrophication of the Canet Lagoon resulted in a simplification of the food web structure (i.e., a single trophic pathway from a ¹⁵N-enriched fraction of the SOM pool to top predators) compared to what was observed in Lapalme Lagoon where additional ¹³C-enriched food sources played a significant trophic role. Moreover, some consumers of Canet tended to exploit primary producers to a larger extent (and thus to exhibit lower trophic levels) than in Lapalme.     

Stable isotope ratios in Cape gannets around the southern coasts of Africa reveal penetration of biogeographic patterns in oceanic signatures

The southern coasts of Africa are influenced by two major oceanic currents, leading to biogeographic patterns in inshore and offshore species assemblages, and in the stable isotope signatures of suspended particulate matter and filter-feeding mussels. We used the stable isotope ratios of carbon (¹³C/¹²C) and nitrogen (¹⁵N/¹⁴N) from the blood and feathers of adult and chick Cape gannets (Morus capensis) to investigate whether the geographic differences observed at the lower levels in the marine communities are deep penetrating effects that reach top predators. Additionally, we evaluated whether trophic segregation occurs between adult and reared chick gannets, and whether a shift to wintering habitat occurs in adults. The study was conducted during the 2006 breeding season on Bird Island in the Agulhas system, and on Malgas and Ichaboe Islands, in the south and north Benguela respectively. Our results showed significant differences in the isotope ratios of members of different colonies, but no intra-colony differences between tissues or age groups. These results indicate that there is neither age-related nor temporal segregation in the diet of members of the same colony. Feather isotopic values suggest that adults remain all year round in the same habitats, and do not undertake long migration after reproduction. Since all gannets tend to target similar prey, we attributed among-colony differences in isotope signatures mostly to the oceanic conditions experienced by the main prey of birds rather than substantial differences in diet composition. Overall, isotopic signatures segregate the two current systems, with depleted carbon values in the Agulhas and enriched nitrogen values in the upwelled waters of the Benguela. Within the Benguela birds from Ichaboe in the north had higher δ¹⁵N values than those from Malgas in the south, which we attributed to differences in the functioning of the upwelling cells in the vicinity of the two colonies. Finally, slight variation in the proportion of main prey and discards from fisheries may contribute to the variation in the stable-isotope signatures between colonies in the Benguela.     

海洋二号微波散射计极地海冰识别算法研究

An Antarctic sea ice identification algorithm on the HY-2A scatterometer(HSCAT) employs backscattering coefficient(σ0) and active polarization ratio(APR) for a preliminary sea ice identification.Then standard deviation(STD) filtering and space filtering are carried out.Finally,it is used to identify sea ice.A process uses a σ0,STD threshold and an APR as sea ice indicators.The sea ice identification results are verified using the sea ice distribution data of the ASMR2 released by the National Snow and Ice Data Center as a reference.The results show very good consistence of sea ice development trends,seasonal changes,area distribution,and sea ice edge distribution of the sea ice identification results obtained by this algorithm relative to the ASMR2 sea ice results.The accuracy of a sea ice coverage is 90.8% versus the ASMR2 sea ice results.This indicates that this algorithm is reliable.     

一株从栉孔扇贝病贝体内分离到的分类地位待定的特殊微生物

从典型患病栉孔扇贝(Chlamys farreri)体内分离到一株特殊微生物，光镜下可见的粒子为革兰氏染色阴性；电镜观察为圆形、近似圆形，已经发现的粒子大部分直径在300nm以下，最小为50nm左右，最大可达4000nm以上。该细菌在人工培养基上形不成肉眼可见的菌落，倒置显微镜下其菌落为无色透明、圆形、小点状菌落。生化特性测试表明该细菌能利用吐温-40与吐温-80。其分类地位尚待进一步分析和证实。     

海水中液-固界面的台阶型动力学曲线 Ⅱ.液膜扩散-递进扩散-界面分级交换反应联合控制的复合模型理论

为定量解释海水中液-固界面的台阶型动力学曲线及其台阶的消长变化规律,在我们已提出的“液膜扩散和递进液膜扩散控制的复合模型”和“海水中液-固界面分级离子/配位子交换理论”基础上,吸取Crank的若干数学处理方法,进而提出适合于非稳态体系的“液膜扩散-递进扩散-界面分级交换反应联合控制的复合模型”。     

赣江上游河流水化学的影响因素及DIC来源

对赣江上游38 处水体采样点的水化学特征和溶解无机碳稳定同位素的分析, 发现其总溶解质浓度较低, 其中, 阳离子以Na⁺、Ca²⁺ 为主, 阴离子以Cl^- 和HCO₃ ^- 为主, Si 的浓度较高, 表征了典型硅酸盐地区河流的水化学组成特征。通过海盐校正分析得出, 研究区大气降水对河水溶解质的贡献率为11.5%, 扣除降水的贡献部分, 利用主成分分析的方法, 计算得出赣南流域受硅酸盐岩风化作用强烈, 同时由于受附近盐矿的影响, 蒸发盐岩的风化作用显著。另外, 根据δ¹³C 溶解无机碳DIC 的测量值约为-8.35‰~-13.74‰, 平均为-11.65‰, 利用质量平衡计算得出, 研究区DIC 的主要来源, 约68.5%来自于土壤CO₂, 31.5%来自于碳酸盐矿物的溶解, 进而得出流域岩石化学风化过程消耗的土壤CO₂ 为2.11×105 mol/yr·km², 来自碳酸盐本身的HCO₃ ^-含量为9.6×104 mol/yr·km²。由于地理位置和流域环境以及人为因素的差异, 各支流DIC 来源的比例亦有所差异。     

Global Statistical Assessment and Cross-Calibration with Jason-2 for Reprocessed HY-2 A Altimeter Data

HY-2 A (Haiyang-2 A) satellite was launched on August 16, 2011 and radar altimeter is one of its main payloads. We reprocessed two years of HY-2 A altimeter sensor geophysical dataset records (SGDR) data. This paper presents the main results in terms of reprocessed HY-2 A altimeter data quality: verification of data availability and validity, monitoring several relevant altimeter parameters, and assessment of the HY-2 A altimeter system performances. A cross-calibration analysis of reprocessed HY-2 A altimeter data with Jason-2 was conducted. The reprocessed HY-2 A altimeter data show good quality and have a low level of noise with respect to Jason-2. The same geophysical correction methods were used to calculate the sea surface height (SSH) for the two missions. The mean standard deviations of the crossover differences for HY-2 A and Jason-2 are 5.24 cm and 5.34 cm, respectively. The mean standard deviation of the crossover differences between HY-2 A and Jason-2 is 5.37 cm. These show that HY-2 A can provide SSH measurements at almost the same level of accuracy as Jason-2. The relative SSH bias between HY-2 A and Jason-2 due to the Ultra Stable Oscillator (USO) drift is obviously observed, and it can affect the calculation of mean sea level and should be further studied and corrected.