壳聚糖酶基因在解脂耶氏酵母中的表达及重组酶性质研究

为构建高效表达壳聚糖酶工程菌株,以Microbacteriumsp.基因组为模板扩增壳聚糖酶基因,目的基因与表达载体pINA1317连接构建重组质粒,重组质粒NotⅠ酶切线性化后转化解脂耶氏酵母Y.liPolytica Po1h。所得结果为PCR扩增得到约1000bp的特异性片段,序列分析表明含有壳聚糖酶全长基因,开放阅读框801bp,编码266个氨基酸残基。转化子酶活力为10.4U/mL,是原菌株酶活力的3.15倍。重组蛋白经DEAE-Sepharose FF分离纯化,达到电泳纯,SDS-PAGE显示单一条带,分子量约41kDa。重组酶反应最适温度为45℃,最适pH为5.6,Km和Vmax分别是0.926mg/mL和6.15U/mL。质谱分析酶解产物主要为三糖和五糖。实现了壳聚糖酶在解脂耶氏酵母中的分泌表达,为壳聚糖酶的工业化生产奠定了理论基础。     

南海海域红斑后海螯虾(Metanephrops thomsoni)的分子识别与群体遗传分化

利用16S rRNA基因序列分子识别红斑后海螯虾,然后对海南附近海域(18°30′-19°00′N,111 °30′-112°30′ E)、北部湾口海域(15°41′ N,110°40′ E)和南沙群岛海域(5°20′-5°29′N,110°09′-111°26′E)三个地理群体进行ITS1的扩增测序.16S rRNA基因序列的结果表明所取样本准确;三个地理群体分别得到616-623bp、619bp和614bp的ITS1全长序列,A、G、T、C含量平均分别为22.3％、29.4％、17.8％和30.6％;其中562个保守位点,39个多态位点.ML和NJ聚类分析显示三个地理群体共聚为三个大支,其中海南群体有群体分化.表明红斑后海螯虾三个地理群体分化极其显著,且海南群体具有相对高的遗传多样性.     

我国海岸潟湖卫星遥感分类方法研究

参照Landsat-5TM和HJ-1CCD遥感影像,依据实地踏勘资料及解译经验,分析各种海岸潟湖地貌学成因和发育演变阶段,提出基于潟湖地貌学成因的遥感分类方法。并将我国海岸潟湖划分为滨外坝型、沙坝型、沙嘴型、湾顶坝型、连岛坝型和河口型共6种地貌类型。建立基于发育演变阶段的潟湖遥感分类标准,将海岸潟湖分为青壮年期、老年期和死亡期共3个发育演变过程;结合潟湖在影像中的特征,以近红外、红、绿波段组合后的假彩色影像为例,从色彩、纹理、地物邻接关系等方面建立不同潟湖类型的遥感解译标志;根据所建立的遥感解译标志,对山东省53个海岸潟湖进行遥感分类验证,结果吻合。     

丁字湾增殖中国对虾适宜量的研究

丁字湾位于山东省海阳、莱阳、即墨三市交界处,面积为１３０ｋｍ２。该海湾局封闭式内湾,不易受风浪影响,地势平坦,潮间带宽阔,水深较浅,最深处１０ｍ。湾内水质肥沃,营养丰富,中国对虾（Ｐｅｎａｅｕｓ　ｃｈｉｎｅｎｓｉｓ喜食的寻氏肌蛤、鸭嘴蛤资源丰厚。该湾春季有亲虾产卵繁殖,６,７月份有幼对虾分布,是进行对虾增殖的优良海湾。但因受饵料和空间等制约,放流数量并非可以无限增加,据１９８８～１９９２年对丁字湾中国对虾放流增殖适宜量的研究表明,随放流数量增加,对虾个体变小,生长减慢,自然死亡率上升。因此,放流…     

Nonlinear Effect of Wave Propagation in Shallow Water

—In this paper,a nonlinear model is presented to describe wave transformation in shallow wat-er with the zero-vorticity equation of wave-number vector and energy conservation equation.Thenonlinear effect due to an empirical dispersion relation(by Hedges)is compared with that of Dalrymple＇sdispersion relation.The model is tested against the laboratory measurements for the case of a submergedelliptical shoal on a slope beach,where both refraction and diffraction are significant.The computation re-sults,compared with those obtained through linear dispersion relation.show that the nonlinear effect ofwave transformation in shallow water is important.And the empirical dispersion relation is suitable for re-searching the nonlinearity of wave in shallow water.     

MOS-1/1b MESSR Observations of the Antarctic Sea Ice: Ice Bands and Ice Streamers

Meso- or submeso-scale features of the Antarctic sea ice are investigated using the MOS-1/1b MESSR Images (spatial resolution of approximately 50 m) received at Syowa Station. Particular attention is paid to the ice bands and ice streamers in coastal polynyas. In the Antarctic Ocean, ice bands can be often seen not only at the ice edge but also in the ice interior zone throughout the year and they extend for hundreds of kilometers in the latitudinal direction. It is found that the width and spacing of ice bands tend to decrease from winter to summer. The width of ice band is about 2–6 km in August and September, and 0.1–0.7 km in December. The spacing of ice bands is about 3–10 km in August and September, and 0.1–2 km in December. In coastal polynyas, ice streamers, which are composed of new ice, are sometimes observed. In general, the row of the streamers is spaced at 0.5–2 km with a width of 0.1–1.0 km.     

海洋光合有效辐射分布的计算模式

针对一类海水，采用美国标准大气模型和一类水体海洋生物-光学特性的半分析方法，建立了海洋光合有效辐射计算模式。模式比较全面地考虑了大气、海-气界面和水体的光辐射传输过程，能较好地用于分析叶绿素浓度以及光辐射场的角分布对光俣有效辐射分布的影响。对模拟结果的分析以及与实测结果的比较表明，该模式能较好地应用于计算衰减系数、光谱辐照度随深度的分布、光合有效辐射随深度的分布和真光层深度，在海洋生态系统的光动力学研究和水色遥感方面有效好的应用前景。     

基于GF-1号的舟山近岸悬浮泥沙浓度反演研究

文章利用舟山近岸实测光谱数据和国产卫星GF-1号宽视场成像仪(WFV)遥感数据,反演该海域悬浮泥沙浓度。结果表明:(1)线性模型和二次模型相比,二次模型的精确度略高于线性模型,但对于悬沙低浓度区,反演误差过大,故线性模型更适用于舟山近岸水体悬浮泥沙浓度反演;(2)GF-1号B2和B3是悬沙浓度变化的敏感波段,B3/B2波段组合的线性模型反演效果较好;(3)GF-1号遥感数据能够较理想地实现定量反演近岸水体的悬浮物。     

利用RADARSAT-1数据提取海水养殖区面积

利用2001年胶州湾RADARSAT-1数据,探讨了SAR影像在海水养殖面积监测方面的可行性。选择适当成像参数、天气与海况等环境条件下成像的遥感数据,进行SAR影像的几何校正、图像增强等图像处理,提取养殖区面积信息。选取胶州湾西北部大沽河口至红岛间的海域作为实验区,对处理后的影像进行养殖区面积的提取,结合常规调查数据对该海域养殖类型变迁分析,结果表明1999到2001年间有4.634×106m2海域由底播养殖区转变成水面养殖区。     

Numerical simulation of random wave slamming on structures in the splash zone

The numerical investigation of random wave slamming on superstructures of marine structures in the splash zone is presented in this paper. The impact pressures on the underside of the structure are computed based on the improved volume of fluid method (VOF). The governing equations are Reynolds time-averaged equations and the two equation k– model. The third order upwind difference scheme is applied to the convection term to reduce the effect of numerical viscosity. The numerical wave flume with random wave-maker suitable for VOF is established. Appropriate moving contact-line boundary conditions are introduced to the model wave in contact with and separated from the underside of structure. Parametric studies have been carried out for different incident waves, structure dimensions and structure clearance. The numerical results are verified by the experimental results.