Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。     

A new pelagic Polyconchoecia Xiang, Chen and Du, 2018 (Ostracoda: Myodocopa: Halocyprididae) from the South China Sea

Halocyprid ostracods are appreciable part of ostracods floating through virtually everywhere in marine environment.In this study,we describe a new species of genus Polyconchoecia Xiang,Chen and Du,2018,tribe Conchoeciini Chavtur and Angel,2011,family Halocyprididae Dana,1853 from the middle of the South China Sea.Polyconchoecia chenii sp.nov.is very close to P.commixtus Xiang,Chen and Du,2018.But it differs from P.commixtus by the distinctions of locations of major glands of carapace and the characteristics of appendages:more posteriorly situated left asymmetric gland of carapace,no right asymmetric gland;segmented frontal organ;the endopod 2 of the first antenna with a very small seta;a-and c-setae of the first antenna with long end joint have long end joint,the b-and d-setae have no end joint,spinose e-seta without end joint;the e-seta of the second antenna is present;teeth side is distinctive;the setal counts of the mandible,maxilla,fifth limb,and sixth limb are individual.The locations of the major glands on carapace and the characteristics of the first antenna can be the key of the new species.This work is the second discovery of the genus Polyconchoecia from the world.     

结合非下采样剪切波变换的BM3D去除图像噪声方法研究

强噪声图像去噪一直是图像处理技术应用领域研究的热点,为进一步提高强噪声图像的去噪质量和对图像边缘的保护,针对三维块匹配（Block Matching 3D,BM3D）方法对强噪声图像去噪效果不佳及图像线状奇异性（如边缘）缺乏最优表示的问题,提出了基于二维非下采样剪切波变换（Non-subsampled Shearlet Transform,NSST）和BM3D的组合去噪方法。该方法首先对含噪图像进行NSST正变换,得到不同尺度和不同方向高频子带的剪切波系数;然后对每个尺度不同方向的高频子带求取贝叶斯阈值,并利用渐进半软阈值函数对各高频子带进行去噪;最后对低频子带和各去噪高频子带进行NSST逆变换,得到去噪结果。将去噪图像作为BM3D中基础估计阶段的预滤波,能够进一步提高基础估计阶段分组的正确性,为BM3D去噪奠定良好基础。组合去噪方法结合了NSST与BM3D的各自优势,仿真试验结果表明,对于低噪声图像,本方法和BM3D方法去噪效果相同,略优于非局部平均法;对于强噪声卫星影像,本方法去噪效果优于BM3D和非局部平均法。     

Research and application on CSAMT constraint inversion

In this paper, according to the method of electrical constraint inversion, we can improve the accuracy of inversion interpretation using the constraint of electrical logging data in the inversion process, which is conducive to obtain more abundant geoelectric information. On the basis of the conventional OCCAM inversion algorithm, the model roughness in the objective function is improved, and the formula of the electrical constraint inversion iterative algorithm is derived in detail based on the modified objective function. Taking the geothermal exploration in Changbai Mountain area as an example, the measured CSAMT data are used as the resistivity model for inversion, and the electrical property is constrained by the actual data from wells drilled in the section. It can be found that the method of electrical constraint inversion can improve the accuracy of inversion interpretation and guide the development of constrained inversion methods in different ways.     

A Raman investigation on the boron form occurring in salt lake brine during evaporation

Boron resources are abundant in Da Qaidam salt lake of Qaidamu Basin. It has been given great attention for the polyborate species present in brine. In this study, the Raman spectroscopy was applied to investigate the existing-form of boron in brine during evaporation. The prepared solutions of MgO·2B2O3-H2O, MgO·2B2O3-MgCl2 -H2O, and MgO·2B2O3-MgSO4-H2O was also evaporated and recorded to study the influence of boron concentration, pH, and electrolytes on the borate speciation in brine. The mononborates of B(OH)3 and B(OH)4- were found to be the only forms present in the original salt lake brine. Brine evaporation promotes the formation of polyborate anions B3O3(OH)4-, B5O6(OH)4-, and B6O7(OH)62- and also disappearance of the B(OH)4- ion in brine with boron concentration of more than 11 g/L in B2O3. The pentaborate ion of B5O6(OH)4- was sensitive to the solution pH and found to be appeared under the pH value of 8.0. While the hexaborate ion of B6O7(OH)62- was observed more dependent on the electrolyte of magnesium chloride due to its special properties, such as promoting boron accumulation, lowering solution pH, and also the strong af?nity for water molecules, which is beneficial to the polymerization of borate ions in brine. The interaction mechanisms among polyborate anions during evaporation had also been proposed.     

虚拟地理环境中基于多模式的多人动作捕捉方法

虚拟地理环境对人类理解、探索地理现象以及发现地理现象之间的联系起到很重要的作用。在基于VR技术的虚拟地理环境中更高效,更自然的交互对虚拟地理环境系统的可用性以及理解、探索地理现象的效率至关重要。当前,人机交互中更自然便捷的交互依赖于动作捕捉技术,而现有的基于单种捕捉模式的动作捕捉方式,易受到遮挡、定位漂移等的影响,捕捉精度受到限制,并只能捕捉单个人的动作,不能满足虚拟地理环境对动作捕捉技术提出的特殊需求。针对单种模式的动作捕捉设备应用于虚拟地理环境受到的限制,本文分析了虚拟地理环境系统中的交互需求以及其对动作捕捉技术提出的特殊需求,创新性的提出了一种基于多种动作捕捉模式以捕捉多人动作的方法。本文方法集中于如何融合基于多种模式的多台设备的动作数据,描述了多人动作捕捉框架及其涉及到的技术,在动作捕捉效果上具有明显提升,能够捕捉多人动作,并开发了原型系统对此方法进行了验证。     

日本“3·11”海啸引起的乐清湾共振研究

本文基于TELEMAC-2D模型建立太平洋区域海啸传播模型,模拟2011年日本“3·11”海啸事件下海啸波的传播。使用实测数据对该模型进行验证,在模型验证良好的基础上分析日本“3·11”海啸事件对乐清湾的影响。通过频谱分析得到“3·11”海啸激发的乐清湾内240、180和103 min这3个主导模态的幅值及其相位。通过白噪声实验对乐清湾的固有共振特征进行估算,进一步支持了乐清湾在上述3个模态发生共振这一结论。白噪声实验还表明,海啸等海洋灾害发生时会在乐清湾湾顶及湾口处产生较大的增水,该结论对乐清湾内海洋灾害风险防范具有指导意义。     

急性高温胁迫对虹鳟二倍体和三倍体幼鱼hsps基因表达的影响

本文以二倍体和三倍体虹鳟(Oncorhynchus mykiss)幼鱼为实验对象,探究急性高温胁迫下,不同倍性虹鳟热休克蛋白家族(hsps)基因表达水平。选取二倍体和三倍体虹鳟幼鱼,分别进行常温处理(14℃)和高温胁迫(22℃),并在高温胁迫48h后,将虹鳟置于常温(14℃)恢复48h。分别在实验开始的0,12,24,48,72和96h进行取样,测定实验虹鳟肝脏和肌肉组织中的hsp70s(hsp70a,hsp70b),hsp90s (hsp90a1a,hsp90a2a,hsp90a1b,hsp90a2a,hsp90b1,hsp90b2)mRNA水平。结果表明:hsp70s和hsp90s的各个亚型均能对热胁迫做出反应,并具有应答幅度的组织特异性和时间特异性。在胁迫6和24h后,上述基因mRNA水平达到最高,随后降低。三倍体幼鱼肝脏和肌肉hsps mRNA表达水平的峰值显著高于二倍体幼鱼。研究结果显示,三倍体虹鳟对高温环境更为敏感,需要消耗更多的能量用于适应高温环境。