Mutations in exons of the CYP17-II gene affect sex steroid concentration in male Japanese flounder (Paralichthys olivaceus)

As a specific gene of fish,cytochrome P450c17-Ⅱ(CYP17-Ⅱ) gene plays a key role in the growth,development and reproduction level of fish.In this study,the single-stranded conformational polymorphism(SSCP) technique was used to characterize polymorphisms within the coding region of CYP17-Ⅱ gene in a population of 75 male Japanese flounder(Paralichthys olivaceus).Three single nucleotide polymorphisms(SNPs) were identified in CYP17-Ⅱ gene of Japanese flounder.They were c.G594A(p.G188R),c.G939A and c.G1502A(p.G490D).SNP1(c.G594A),located in exon 4 of CYP17-Ⅱ gene,was significantly associated with gonadosomatic index(GSI).Individuals with genotype GG of SNP1 had significantly lower GSI(P < 0.05) than those with geno-type AA or AG.SNP2(c.G939A) located at the CpG island of CYP17-Ⅱ gene.The mutation changed the methylation of exon 6.Indi-viduals with genotype AA of SNP2 had significantly lower serum testosterone(T) level and hepatosomatic index(HSI) compared to those with genotype GG.The results suggested that SNP2 could influence the reproductive endocrine of male Japanese flounder.How-ever,the SNP3(c.G1502A) located in exon 9 did not affect the four measured reproductive traits.This study showed that CYP17-Ⅱ gene could be a potentially useful candidate gene for the research of genetic breeding and physiological aspects of Japanese flounder.     

壬基酚对罗氏沼虾血清中免疫酶活力的影响

将罗氏沼虾(Macrobrachium rosenbergii)在0.1、0.2、0.4 mg/L的壬基酚中暴露48 h,检测壬基酚对罗氏沼虾血清中部分免疫酶活力的影响。结果表明:高剂量壬基酚组(0.4 mg/L)的罗氏沼虾血清超氧化物歧化酶(SOD)活力在前期受到明显抑制(p<0.05),24 h后各剂量组血清SOD活力转为促进,中、高剂量组的SOD活力显著高于对照组(p<0.05);中、低剂量组血清中酸性磷酸酶(ACP)活力均受到抑制,其中12 h低剂量组血清ACP活力显著降低(p<0.05),高剂量组则表现为先促进后抑制的效应;各剂量组血清碱性磷酸酶(AKP)活力先受到抑制后转为促进,48 h中、低剂量组血清AKP活力显著高于对照组(p<0.05)。     

漳州地震台TJ-Ⅱ型体应变观测初析

通过对漳州地震台TJ-Ⅱ型体应变资料的映震效应分析,并利用多种方法对形变前兆异常分析,探讨区域地下形变资料对地震短临预报的实际意义。     

辽宁省地震重点监视防御区Ⅱ类场地放大效应研究

利用辽宁省地震重点监视防御区工程场地地震安全性评价工作中积累的大量实际钻孔和土工试验资料,在50年超越概率为63%、10%、2%三种地震动输入情形下,采用等效线性化方法研究了辽宁省地震重点监视防御区Ⅱ类场地土层的放大效应。研究结果表明辽宁省地震重点监视防御区50年超越概率10%的平均场地效应放大因子为1.37,若使用《中国地震动参数区划图（GB18306-2001）》认定的系数（1.25）将低估研究区域内的场地放大效应。50年超越概率2%情形即＂工程结构抗倒塌水准＂的平均场地放大效应因子为1.35。这些结果有益于为研究区域提供更为合理的抗震设防标准。研究同时发现,实际工程中使用单一变量或有限的几个变量去控制场地放大效应会有很大的离散,因此重大工程项目的地震安全性评价工作是不可被替代的。     

Reflectance spectral characterization and mineralogy of acid sulphate soil in subsurface using hypcrspectral data

Acid sulphate soil（ASS） is a kind of soil which is harmful to the environment.ASS is hard to efficiently assess efficiently in the subsurface,although it is detectable on the surface by remote sensing.This paper aims to explore a new way to rapidly assess ASS in the subsurface by introducing a proximal hyperspectral instrument,namely the HyloggerTM system which can rapidly scan soil cores and provide high resolution hyperspectral data.Some minerals in ASS,which usually act as indicators of the severity of ASS,such as iron oxides,hydroxides,and sulphates,as well as some clay minerals,such as kaolinite,have diagnostic spectral absorption features in the reflectance spectral range（400-2500 nm）.Soil cores were collected from a study area and hyperspectral data were acquired by HyloggerTM scanning.The main minerals related to ASS were characterized spectrally,and were subsequently identified and mapped in the soil cores based on their reflectance spectral characteristics.Traditional X-ray diffraction（XRD） and scanning electron microscope（SEM） were also applied to verify the results of the mineral identification.The main results of this study include the spectral characterisation of ASS and its main compositional minerals,as well as the distribution of these relevant minerals in different depth of cores.     

Study on the Determination of Trace Ni（Ⅱ） by the Catalytic Kinetic Spectrophotometric Method

A new kinetic spectrophotometric method has been developed for the determination of trace Ni (II) in natural water.The method is based on the catalytic effect of Ni (II) on the oxidation of weak acid brilliant blue dye (RAWL) by KIO4 in acid medium.The concentration of nickel (II) can be determined spectrophotometrically by measuring the decrease of absorbance of RAWL at λ= 626 nm using the fix-time method.The influencing factors are investigated by the orthogonal experimental design.The obtained optimum analytical conditions are:pH=2.00,c RAWL=5.00×10-5 mol L-1,cKIO 4= 2.00×10-5 mol L-1,the reaction time t=10 min and the temperature T=25℃..Under the optimum conditions,the developed method allows the measurement of Ni (II) in a range of 0-40.0 ng mL-1.The standard deviation of eleven independent measurements of blank reaction is S=3.08×10-3 and the limit of detection is 2.20 ng mL-1.The relative standard deviations (RSDs) in six replicate determinations of 5 ng mL-1 and 8 ng mL-1 Ni (II) are 2.87% and 1.11%,respectively.Moreover,the experiments show few cations and anions can interfere with the measurement of Ni (II).The recovery efficiencies of this method are in a range of 97.0%-102.5% in freshwater samples.But there is a decreasing effect,which is about 0.2 times the added Ni (II) in seawater medium.After reasonable calibration this processing method is used for the determination of Ni (II) in seawater samples successfully.The results show this developed method has high accuracy and precision,high sensitivity,large range of linearity and high speed.The method can,therefore,be employed at room temperature.     

六安地震台TJ-Ⅱ井下体应变观测与资料初析

介绍了六安地震台TJ-Ⅱ体应变仪安装井的地质构造特点与井孔岩芯物理特性,以及TJ-Ⅱ体应变仪在六安地震台的运行情况。通过对六安台TJ-Ⅱ一年多来观测资料映震效应与区域前兆异常分析,初步分析了区域地下形变对大别山区未来中强地震预测研究的实际意义。     

陕西地震前兆台网ZD9A-Ⅱ地电场仪故障及排除

通过对陕西地震前兆台网4个地震台的ZD9A-Ⅱ地电场仪的观测资料进行了分析,对该仪器的各项工作指标、观测记录的数据甄别出异常变化形态,找出观测过程中存在的主要问题和故障现象,分析发生仪器故障的原因及处理方法;对有关的电路及故障原因进行分析,并以实例对这些故障的检修进行了总结,提出了检修方法、步骤及改进措施。     

编码激励用于岩石物性超声成像的仿真

数字编码技术在雷达、医学等方面广泛成功应用使得将其引入地震探测中成为可能。本文为将数字编码技术引入地震探测中,进行了岩石物性成像仿真实验。首先讨论了编码超声成像原理,分析了编码技术的规律和特点。其次通过激励Golay码,利用FieldⅡ软件包进行了岩石物性超声成像的仿真,并比较了仿真结果的成像分辨率。结果表明,编码技术是提高超声成像质量的有效方法。结果可用于指导野外编码地震探测。     

内蒙古朱拉扎嘎金矿火山岩岩(矿)相学及成矿意义

笔者对内蒙古朱拉扎嘎金矿做了详细的镜下岩（矿）相学研究工作,首次发现火山岩（包括石英角斑岩,粗面流纹岩,团粒状充纹岩,变矿化流纹岩,蚀变霏细岩等）和其他火山活动的痕迹。改变了过去人们对该矿床成因的认识。火山活动不仅带来了大量熔浆热物质,而且带带来了大量的成矿金属元素,为矿床的形成准备了丰富的物质基础和良好的成矿环境。因此,与中元古代朱拉扎嘎毛道组地层沉积同期发生的火山作用对朱拉扎嘎金矿的形成起着重要的作用。