Effect of Bacillus baekryungensis YD13 Supplemented in Diets on Growth Performance and Immune Response of Sea Cucumber（Apostichopus japonicus）

The effect of a potential probiotic on the growth performance and immune response of sea cucumber（Apostichopus japonicus） was investigated. Bacillus baekryungensis YD13 isolated from sea cucumber culturing ponds was added to sea cucumber basal feed as a probiotic in different doses（0, the control; 1×10^4（YD134）, 1×10^6（YD136） and 1×10^8（YD138） CFU g-1 of diet）, and administered orally to A. japonicus（initial mean wet weight 5.44 g ± 0.17 g）. The sea cucumbers were fed in 20 aquaria, 5 each treatment, for 60 d. At the end of growth trial, 20 sea cucumbers from each treatment were challenged with Vibrio splendidus. A. japonicus in YD134 and YD136 exhibited significantly better growth performance than control（P 〈0.05）. Five non-specific immune parameters including lysozyme, acid phosphatase, alkaline phosphatase, superoxide dismutase and catalase in coelomic fluid were measured to evaluate the immune response of A. japonicus to the probiotics. Results showed that all parameters were significantly improved when YD11 was supplemented in the dose of 1×10^6 CFU g^-1（P 〈0.05）. The cumulative incidence and mortality after the Vibrio splendidus challenge decreased significantly in sea cucumbers of YD136. Accordingly, 1×10^6 CFU g^-1 of YD13 in diet was recommended for the growth promotion and immune enhancement of A. japonicus.     

巩义市开展打击非法采矿专项行动

正为有效打击无证开采和超层越界违法开采行为,促进矿产资源开发秩序和矿山安全生产形势进一步好转,巩义市从6月1日起至10月20日,在全市范围内开展打击非法开采和超层越界违法开采专项行动。据悉,此次专项行动检查的范围是全市持证矿山企业和各类无证开采矿点,重点是有超层越界查处记录和嫌疑的矿山企业。排查治理分自查自纠(2014年6月1日至7月1日)、集中排查(7月2日至8月1日)、集中整治(8月2日至10月1日)、总结完善四个阶段进行。     

设定国家宪法日有助于提高宪法观念

从我国宪法实施的总体状况来看,执政党一直将宪法作为一个重要的政治文件,因此政治化实施是宪法实施的一个重要特点。     

四川盆地东部垫江盐盆早三叠世嘉陵江组四段杂卤石成因及对成钾的指示

四川盆地三叠纪是主要的成钾期,目前发现的主要含钾矿物为杂卤石,有关早三叠世嘉陵江组四段石盐岩中杂卤石成因一直存在争议.采用薄片鉴定、扫描电镜、稀土元素和锶同位素等手段,分析了四川盆地东部垫江盐盆长寿地区嘉陵江组四段石盐岩中杂卤石矿物形态特征,初步探讨了该杂卤石成因及对寻找海相钾盐的指示意义.扫描电镜下与石盐岩共生的杂卤...     

江苏南部汛期降水日变化特征分析

利用江苏南部20个气象观测站2008—2012年汛期(5—10月)逐小时降水资料,应用降水频率来分析了江苏南部地区降水日变化基本特征和区域差异。研究表明:降水日变化特征地域性差异较强,西部站、东部站和东北沿海站都存在一定的特征差异。东部站降水量的最大值主要出现在下午和傍晚;西部站降水量主峰值出现在下午,并且在清晨和夜间还有两个次峰值;东北沿海站呈现出午前、午后的双峰值形式。2008—2011年降水量下午高值区有先减弱后增强并提前的趋势,而上午的高值区有总体减弱并推迟的特征。2011年后有明显减弱的趋势。江苏南部总体来说,短时强降水(大于20和25 mm/h)在16—19时出现主峰值,07—09时也有相对较小的次峰值。     

青蛤(Cyclina sinensis)TLR2基因的克隆与表达分析

利用转录组文库分析得到青蛤(Cyclina sinensis)的Toll样受体2(Toll-like receptor 2,TLR2)基因序列,采用荧光定量PCR法分析该基因的表达过程。结果显示,青蛤TLR2基因的cDNA开放阅读框为2082bp,编码693个氨基酸,具典型的LRRs、单次跨膜区和TIR结构域。该基因在血淋巴中的表达量最高,与肝脏、鳃、外套膜、闭壳肌和性腺有显著性差异(P0.05)。在鳗弧菌和Poly I:C刺激下,青蛤血淋巴中TLR2基因3h开始升高,48h达到最大值,与对照组和空白组有极显著性差异(P0.01);藤黄微球菌刺激下,血淋巴中CsTLR2基因3h开始升高,48h亦达到最大值,实验组与对照组有显著性差异(P0.05)。     

Phylogenomic analysis of transcriptomic sequences of mitochondria and chloroplasts for marine red algae (Rhodophyta) in China

The chloroplast and mitochondrion of red algae （Phylum Rhodophyta） may have originated from different endosymbiosis. In this study, we carried out phylogenomic analysis to distinguish their evolutionary lin-eages by using red algal RNA-seq datasets of the 1 000 Plants （1KP） Project and publicly available complete genomes of mitochondria and chloroplasts of Rhodophyta. We have found that red algae were divided into three clades of orders, Florideophyceae, Bangiophyceae and Cyanidiophyceae. Taxonomy resolution for Class Florideophyceae showed that Order Gigartinales was close to Order Halymeniales, while Order Graci-lariales was in a clade of Order Ceramials. We confirmed Prionitis divaricata （Family Halymeniaceae） was closely related to the clade of Order Gracilariales, rather than to genus Grateloupia of Order Halymeniales as reported before. Furthermore, we found both mitochondrial and chloroplastic genes in Rhodophyta under negative selection （Ka/Ks〈1）, suggesting that red algae, as one primitive group of eukaryotic algae, might share joint evolutionary history with these two organelles for a long time, although we identified some dif-ferences in their phylogenetic trees. Our analysis provided the basic phylogenetic relationships of red algae, and demonstrated their potential ability to study endosymbiotic events.     

中海达杯第三届全国高等学校大学生测绘技能大赛落幕

正[本刊讯]7月18日至21日,中海达杯第三届全国高等学校大学生测绘技能大赛在河南平顶山举行,共有来自全国76所高校、317名学生参与角逐,并充分展示了自己的测绘技能水平和实际操作能力,创下了大赛举办以来的参赛人数纪录,受到裁判与学生选手的一致好评。     

Progress in Studies of the Precipitation Diurnal Variation over Contiguous China

This paper summarizes the recent progress in studies of the diurnal variation of precipitation over con- tiguous China. The main results are as follows. （1） The rainfall diurnal variation over contiguous China presents distinct regional features. In summer, precipitation peaks in the late afternoon over the south- ern inland China and northeastern China, while it peaks around midnight over southwestern China. In the upper and middle reaches of Yangtze River valley, precipitation occurs mostly in the early morning. Summer precipitation over the central eastern China （most regions of the Tibetan Plateau） has two diurnal peaks, i.e., one in the early morning （midnight） and the other in the late afternoon. （2） The rainfall diurnal variation experiences obvious seasonal and sub-seasonal evolutions. In cold seasons, the regional contrast of rainfall diurnal peaks decreases, with an early morning maximum over most of the southern China. Over the central eastern China, diurnal monsoon rainfall shows sub-seasonal variations with the movement of summer monsoon systems. The rainfall peak mainly occurs in the early morning （late afternoon） during the active （break） monsoon period. （3） Cloud properties and occurrence time of rainfall diurnal peaks are different for long- and short-duration rainfall events. Long-duration rainfall events are dominated by strat- iform precipitation, with the maximum surface rain rate and the highest profile occurring in the late night to early morning, while short-duration rainfall events are more related to convective precipitation, with the maximum surface rain rate and the highest profile occurring between the late afternoon and early night. （4） The rainfall diurnal variation is influenced by multi-scale mountain-valley and land-sea breezes as well as large-scale atmospheric circulation, and involves complicated formation and evolution of cloud and rainfall systems. The diurnal cycle of winds in the lower troposphere also contributes to the regional differences