日照市东南部地区榴辉岩矿矿石加工技术性能及选矿工艺研究

日照市东南部岚山安东卫、虎山地区位于苏鲁榴辉岩带南西段的中部,区域内赋存大型榴辉岩矿体,榴辉岩资源较丰富。榴辉岩含有的石榴子石、绿辉石、金红石都是应用很广的矿物,但榴辉岩的选矿工艺比较复杂,特别是其中金红石的选矿分离一直难以解决。该文就日照东南部地区的榴辉岩矿石的加工技术性能及选矿工艺进行了试验和探讨研究,为今后该区榴辉岩矿的开发利用打下了一定基础。     

萃取分离硫代米氏酮目视比色法快速测定岩石矿物中的金

结合野外现场的条件,经试验提出了用甲基异丁基酮-异戊醇萃取分离,硫代米氏酮目视比色法快速测试地质样品中的金,该方法适宜于矿石中痕量金的测定,方法简便、实用、稳定,结果满意。     

Genetic study of Kelp “901” strain

Based on DNA extraction and optimization of random amplified reaction (RAPD) to the gametophytes and sporophytes of Kelp “901” strain, genetic study on variation was conducted to its parents and offsprings of F6, F7,Fs, and F9 generation. RAPD results have shown that among 30 selected primers for gametophytes, 297 loci ranging from 200 to 3 000 bp were obtained in the average of 9,9 loci for each primer, This indicated a high polymorphic rate with RAPD detection. UPGMA (unweighted pair-group method arithmetic average) analysis showed that each male and female gametophyte of a generation could be clustered into one pair separately. The genetic distances of the Kelp 901 generation were 0.321 2-0.476 7, and the maximum was between F7 and Fs (0.476 7). Identity analysis showed that F6 generation was more close to the female parent (0.659 3), and F7 generation was more close to the male parent (0.5788). To the sporophytes study in 24 selected primers for RAPD amplification, 191 loci ranging from 230-2 800 bp were obtained, in the average to each primer of 8.0 loci. The heterozygosity to six populations were male parent (0.223 9),female parent (0.107 2), F6 (0.216 4), F7 (0.228 6), F8 (0.229 6) and F9 (0.317 2). The nearest genetic distance was 0.083 5 (Fs, F9). Total heterozygosity (HT) ofF6, F7, F8 and F9 generations was 0.318 6, the average heterozygosity (Hs) for F6,F7, F8 and F9 generations was 0.248 0, and deduced coefficient of population differentiation (Gst) was 22.2%. Six sequence characterized amplified regions (SCAR) were preliminary screened through RAPD analysis. It needed to be verified in detail as they are significant for molecular marker assistance in breeding and selecting Laminaria.     

Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits in Fenneropenaeus chinensis

Amplified fragment length polymorphisms （AFLP） technique was used to analyze the fingerprinting of four successive generations of Fenneropenaeus chinensis to reveal their disease-resistance traits. Some loci showed quite different genetic frequencies due to artificial selection, which implied that these fragments were putative markers related to the disease-resistance trait. We developed a simple and effective method to further characterize these AFLP fragments. Specific AFLP bands were cut directly from polyacrylamide gels, re-amplified, cloned and sequenced. Eight putative genetic markers were sequenced and their sizes ranged from 63 to 209 bp. The sequences were submitted to dbGSS （database of Genome Sequence Survey）; and the BLAST analysis showed low similarity to the function genes, indicating these markers were tightly linked to a disease-resistance trait but were not functional genes.     

黑龙江省蛋白石综合开发应用工艺研究

黑龙江省蛋白石（硅质页岩）赋存于上白垩统嫩江组中一个较稳定的层位，储量可达几千万吨，广泛地分布在嫩江、讷河、德都、望奎、嘉荫以及宁安、北安、庆安等市县。该岩石富孔隙，具有很高的比表面积，高硅、低铝，具特异的212业物理性能，在石油、化212、建材等领域有着广泛的应用前景。     

嘎玛藏族首饰的技艺特色

首饰具有浓厚的文化色彩,其所体现出来的特性可以反映出一个民族的审美特征。噶玛藏族首饰技艺和其他民族首饰一样,具有很强的审美功能和独特的造型语言。噶玛藏族传统首饰也是藏族历史文化发展、演绎的投射和噶玛藏族多元一体文化的智慧结晶。     

油炸温度和时间对克氏原螯虾虾肉贮藏品质的影响

【目的】分析常压油炸工艺对克氏原鳌虾(Procambarus clarkii)虾肉贮藏期间的理化性质及品质的影响。【方法】以虾肉为研究对象,经不同油炸温度(160、170、180、190℃)和油炸时间(30、60、90、120 s)处理,探究4℃贮藏1～7 d虾肉菌落总数(TPC)、挥发性盐基氮(TVB-N)、质构特性等指标的变化。【结果与结论】随着油炸温度升高和油炸时间延长,虾肉TPC和持水力显著降低(P<0.05);硬度、弹性、咀嚼性、TVB-N值等均增加(P <0.05)。其中,贮藏1～7 d,油炸组样品TPC对数值最高为4.95,且TVB-N值均低于淡水虾限量值(20.00 mg/100 g)。少于油炸时间90 s(30 s、60 s)和低于油炸温度180℃(160℃、170℃)处理的虾持水力较高,硬度和咀嚼性略低,且贮藏5～7 d时,L^*值和a^*值较低,b^*值较高;120 s和190℃处理的虾持水力显著较低,虾肉色度下降,TVB-N和TBA值较高,质构特性值(硬度、弹性、咀嚼性)整体较高。主成分分析...     

离线固相萃取螯合富集分离-ICP-MS测定海水中的稀土元素

通过测定条件优化、方法比对等实验建立了一种固相萃取小柱离线螯合富集分离电感耦合等离子体质谱仪测定海水中稀土元素的方法。海水样品通过调节p H后,进入VAC ELUT SPS24 Agilent圆形固相萃取装置,其主要基体物质的去除率高于97%;萃取富集的优化条件是海水样品p H 4.0~7.0,海水进入萃取柱速率2 m L/min,硝酸洗脱液浓度为1 mol/L;方法对稀土元素的加标回收率为83%~108%,14种稀土元素的检出限为0.057~0.613 ng/L,RSD10%;该方法与氢氧化铁共沉淀法富集稀土元素比对测定结果一致,方法具有准确度与精密度高、操作简便快速等优点,可用于海水样品中稀土元素的定量精确测量。     

正交实验法优化紫蛇尾皂苷提取工艺研究

以紫蛇尾(Ophiopholis mirabilis)皂苷溶液吸光度为指标,采用单因素实验法考察乙醇体积分数、料液比、提取时间、提取次数、提取温度等因素对紫蛇尾皂苷提取效果的影响,通过正交实验确定最佳工艺条件。结果表明,紫蛇尾皂苷的最佳提取工艺为60℃水浴,料液比1:40(W/V),每次3 h,提取4次。按照最佳工艺条件所得蛇尾皂苷平均产率为1.77‰,表明此最佳工艺可用于紫蛇尾皂苷的制备。     

鄂尔多斯盆地东部深部煤层气井压裂工艺及实践

鄂尔多斯盆地东部深部煤层气（埋深大于1 200 m）资源丰富,是下步煤层气勘探、开发的重点。与浅部煤层压裂相比,深部煤层压裂存在诸多挑战（地应力、闭合应力、破裂压力高,岩石力学参数复杂等）。通过对比现有煤层压裂工艺,以鄂尔多斯盆地东部深部煤层（埋深约2 000 m）为例,探索了采用油管+循环滑套+封隔器+喇叭口施工管柱和低浓度线性胶压裂液体系对深部煤层进行压裂作业,施工比较顺利,见气快,产气效果比较理想（产气量大于800 m³/d）。通过2口井的现场作业实践,证明了该工艺技术简单、易行、有效,为后续深部煤层气的勘探开发提供可借鉴的工艺技术。