基于河口潮间带底栖微藻群落水平的环境质量状态评价

The feasibility of community-based bioassessment of environmental quality status was studied using microphytobenthos(MPB) in estuarine intertidal ecosystems.The sediment samples of MPB were collected monthly during a 1-year cycle(September 2006–August 2007) at four sampling stations in the Nakdong River Estuary,Korea.Environmental variables,such as salinity,radiation,grain size of sediment,Si(OH)_4(Si),nitrate(NO_3~–),nitrite(NO_2~–),ammonium(NH_4~+) and phosphates(PO_4~(3–)),were measured synchronously for comparison with biotic parameters.The statistical analyses were carried out for assessment the relationship between biotic and environmental parameters.The results showed that:(1) the MPB community structures were significant differences among four sampling stations;(2) spatial variation in the MPB communities were significantly correlated with environmental variables,especially the nutrient NH_4+ in combination with salinity and grain size;(3) three species(Navicula lacustris,Pleurosigma anglulatum and Fragilaria sp.1) were significantly correlated with nutrients and/or Si;and(4) the species richness and diversity were significantly correlated with the grain size.It is suggested that MPB communities may be used as a potentially robust bioindicator for assessing environmental quality status in estuarine intertidal ecosystems.     

预处理方法对海洋微藻Isochrysis zhanjiangenisis脂肪酸组成及稳定碳同位素的影响

This study aims to quantify the effects of different pretreatment methods on the stable carbon isotope values of fatty acids in marine microalgae(Isochrysis zhanjiangenisis).To identify the effects of sample preparation on theδ~(13)C value and the fatty acid composition,we examined eight types of pretreatment methods including:(a) drying the sample followed by direct methyl esterification using HCl-CH_3OH;(b) drying the sample followed by direct methyl esterification using H_2SO_4-CH_3OH;(c) drying the sample by ultrasonic extraction and methylesterification using HCl-CH_3OH;(d) drying the sample by ultrasonic extraction and methyl-esterification using H_2SO_4-CH_3OH;(e) fresh sample followed by direct methyl-esterification using HCl-CH_3OH;(f) fresh sample followed by direct methyl-esterification using H_2SO_4-CH_3OH;(g) fresh sample with ultrasonic extraction followed by methyl-esterification using HCl-CH_3OH,and(h) fresh sample with ultrasonic extraction followed by methylesterification using H_2SO_4-CH_3 OH.The results show that the δ~(13)C values from Groups a–e,g and h fluctuated within 0.3‰,and the δ~(13)C values of Group f were approximately 0.7‰ lower than the other seven groups.Therefore,the different sample pretreatment methods used towards the extraction of fatty acids from marine microalgae may result in different results regarding the stable carbon isotope ratios,and if necessary a correction should be applied.     

基于人为调控盐度的促进大叶藻种子萌发及建苗技术研究

Vast declines in Zostera marina seagrass beds demand effective methods of rehabilitation. In this study, we developed a practical method by reducing salinity to induce seed germination followed with recovering salinity to facilitate seedling production of Z. marina. The results showed that Z. marina seeds collected from natural seawater(salinity 30) were induced to germinate at reduced salinities. Percent germination(GR) was higher and mean-time-to-germinate(MTG) was shorter at lower salinities. The highest GR and shortest MTG occurred at salinity 0(deionized freshwater). After germination in freshwater, seeds could develop into seedlings at salinities5–30 and continue the growth. Viability or development of germinated seeds was not significantly different during the 40 d of post-germination incubation at salinities 5–15 after 1–20 d of germination in freshwater. However,during the process of translating germinated seeds from salinity 0 and 5 to salinity 30, reducing the gradients of post-germination acclimation facilitated more seeds forming seedlings in less time. On average, after 60 d of static incubation, including 20 d in freshwater for germination followed with immediate shift to salinity 5 and increasing to salinity 30 at increment of 5 every two days until cultivation at constant salinity 30, 33% of Z. marina seeds produced healthy seedlings. The results indicate that the salinity-manipulation based method of artificial germination and seedling production is practical and effective in supporting rehabilitation of Z. marina bed.     

两种海洋微藻的浓度和配比对太平洋纺锤水蚤生长的影响

本研究以变态存活率、产卵量、成体头胸部长和体长为指标,研究了球等鞭金藻(Isochrysis galbana)和海洋原甲藻(Prorocentrum micans)不同浓度和配比对太平洋纺锤水蚤(Acartia pacifica)生长发育和产卵量的影响,以期探索培养太平洋纺锤水蚤最适合的饵料配方.实验结果表明:在饵料浓度(碳含量,下同)为1.70μg/cm3,配比为2∶1时,其变态存活率最高,可达到92.62%;在饵料浓度为4.25μg/cm3,配比为1∶2时,产卵量最高,雌体平均日产卵量可达47.1±2.7个;在饵料浓度为1.70μg/cm3,配比为2∶1时,成体头胸部长和体长均最长,分别为932.9、1 252.3μm.太平洋纺锤水蚤生长和发育的最优饵料组合为球等鞭金藻与海洋原甲藻浓度为1.70μg/cm3,配比为2∶1.     

墨西哥湾深水油气圈闭类型与成藏模式

墨西哥湾深水作为全球深水勘探的热点盆地之一,日益成为各大石油公司竞逐的勘探领域。通过对其石油地质条件以及圈闭类型和成藏模式的探讨,指出其圈闭勘探呈明显的分带性,且平面分布特征明显;主要圈闭类型通常可分为4-way和3-way两大类,但以3-way类型的居多;墨西哥湾深水勘探目标的关键成藏要素为圈闭、储层和油气运移,但对于不同的勘探区带,其侧重点各有不同;通过对3个勘探区带的关键成藏要素的比较,指出从成藏条件来看,东部侏罗系风成砂勘探区带优于古近系Wilcox勘探区带,中新统勘探区带稍逊于古近系Wilcox勘探区带。     

巴丹吉林沙漠沙山区径流与地下水补给条件

在全球高差最大的巴丹吉林沙漠沙山斜坡上发现了沙漠区罕见的因大气降水而产生的地表超渗径流,地表径流侵蚀,缓渗径流及因地下径流出露而产生的地表径流--风沙混合物理沉积与化学沉积(次生盐).根据电镜观察,能谱分析,化学分析和粒度分析等资料,研究了径流化学沉积物的矿物和化学组成,风积沙和径流--风沙混合物理沉积物的粒度组成,径流类型及沙山区水分平衡,降水对地下水与湖水的补给机制.超渗径流的出现表明,虽然该区降水量少,但存在能够为地下水提供补给来源的较强有效降雨过程.径流,径流--风沙混合物理沉积和化学沉积等六项科学指标从深层次上充分证明,该区降水通过入渗至少达到了沙山的下部,完全能够对地下水构成有效补给.细粒层相对的隔水性是该区地下径流出露于地表的原因.沙山区确实存在令人惊奇的水分正平衡,即在大气降水在经过蒸发和蒸腾等消耗之后,每年还有约134648.4 t/km²水补给了地下水和湖水,成为湖水的重要补给来源之一.沙山区能够产生有效降水,植被稀少蒸腾量少,沙层入渗率高和沙层受蒸发影响深度很小是该区水分出现正平衡和大气降水能够补给地下水的四个条件,也是导致该沙漠区有众多湖泊发育的主要条件.     

南海大鹏湾盐田海域骨条藻数量的多元分析

１９９０年及１９９１年３月３０日－５月３０日，在南海大鹏湾盐田海域设点测定骨条藻数量及各环境要素，以逐步回归，主成分分析及判别分析的方法，分析该海区骨条藻数量与环境因子的关系。１９９０年４、５月与１９９１年４，５月骨条藻数量差异与该期间环境因子值差异显著有关；初步提出用三维主成分分析图及判别分析方程作为盐田海域骨条材料潮预报的方法。     

存贮对海洋微藻活性和脂肪酸分布影响的综述

在饲养许多水生动物时，微藻常常用来作为幼虫饵料，因此培养鲜活做藻就显得十分重要。由于环境条件的不利或其它生物体的污染，而导致微藻培养的失败，常常引起培养的幼虫在发育到商品规格前就食物链中断。如能够提供给孵化场以优质的存贮微藻，对于水产养殖研究和商业孵化场都有十分重要的意义。目前X国学者都已将注意力集中到选择微藻及其存贮技术的研究中（其中保存方法如：冻干存贮、在液态氮中存贮和冷冻浓缩存贮等）。用于海洋动物幼虫和幼年期培养的微藻，其营养价值是和多种不饱和脂肪酸计UI；A）的含量有关的。因此存贮方法必须…     

三角褐指藻(Phaeodactylum tricornutum)形态及其变异现象的研究

分别对青岛、浙江、上海和厦门四地的三角褐指藻藻种及单个细胞克隆后多个藻株的形态及其变异现象进行了比较研究。青岛、上海藻种短期培养后，培养液细胞基本由梭形细胞组成；浙江和厦门藻种则基本由三出放射形细胞组成。正常培养液中卵形细胞比例均在0.1%以下。特定条件下，梭形和三出放射形细胞均能产生卵形细胞，卵形细胞也能发育成梭形或三出放射形细胞；三出放射形细胞可能经“突变”或“定向变异”转变成梭形细胞，而梭形细胞培养液中没有出现三出放射形细胞。培养液比重、温度及光照条件的改变短期内对细胞形态类型的转变没有明显影响；淡水和低温(4℃～8℃)的长期胁迫能使梭形和三出放射形藻株发生高比例的卵形细胞。     

论盐、碱、镁的联产问题

从可能性和可行性两个方面论证了盐、碱、镁的联产问题。强调了利用莱州湾沿岸地下浓缩海水和饱和盐水的预处理方法以及白泥(盐泥)的综合利用问题,并着重指出了环境问题的重要性。最后提出了盐、碱、镁联产中应注意的几个问题。