广西百色水电站边坡治理工程

文章通过广西右江百色水利枢纽地下发电厂房进水口与尾水洞高边坡的治理，介绍在此工程中具有特殊性的一些施工方法。该工程地质体为弱风化硅质泥岩夹泥化夹层、强风化含洞穴硅质泥岩石、强风化硅质岩及全强风化辉绿岩，地质条件非常复杂，对坡体的稳定极其不利。根据三维有限元的分析计算和国内有关权威专家的调查研究需施工1500kN、1000kN的锚索方能保证坡体的永久稳定。大吨位的锚索需锚固在强风化岩土体上，锚索设计采用能提高锚固力的防腐型压力分散锚索。通过现场锚索基本试验，其锚固力完全能满足工程需要。为保证进水口总体施工的进度，在施工进水口约30m高的垂直壁上的锚索时，笔者采用了通常施工中较少采用的垂直壁悬吊式施工法，满足了水电站的整体施工进度和要求。     

云南个（旧）-冷（墩）公路K22＋336回头湾段边坡治理工程研究

目前，在我国地质灾害防治及道路建设工程中，岩土锚固施工大多采用单一的拉力型锚索工法。一般锚索索体的长效防腐问题没有得到应有的重视。文章对云南省个旧-冷墩公路边坡工程中所采用的压力分散型锚索工法从设计、试验及成功应用等多方面进行了详细介绍。①在一些复杂地形条件下，锚索防护方案有其独特的经济实用性。②在地基承载力较低的软弱地层中，常用的拉力型锚索无法提供防护工程所需的大吨位锚固力。而有多个受力体的压力分散型锚索则因其对锚固段全长范围内地基承载力的充分利用来提供较大吨位的锚固力；③永久锚索防护工程作为一项具有隐蔽性强特点的结构工程，为保证在其报务年限内锚索能够可靠地工作，在设计阶段，锚索体的长效防腐应为不能忽略的主要考虑因素。在该工程中首次应用了新型高强材料——环氧全喷涂无粘结钢绞线作为锚索体的杆材，较好地解决了永久锚固工程中的锚索防腐问题。     

蜱虫致死原因：新致命病毒

蜱虫是去年最令中国人恐慌的一种虫子，现在研究人员已经发现了蜱虫盯咬致死的原因。     

南海海岛自动气象站防护应用技术探索

立足海南及南海的自然条件,结合海岛自动气象站在南海高温、高湿、高盐雾、强辐射、强风等恶劣气候环境下使用情况,通过对历年来海岛自动气象站故障及失效模式的统计和分析,总结分析故障原因。根据设备维护人员多年气象设备保障工作积累总结的经验,从海岛自动气象站结构和防护要求出发,集成应用市场上优质材料、先进工艺和多种成熟、关键设备防护技术,形成一些气象设备防护的有效方法措施,以提高海岛自动气象站对海洋环境的适应性和性能,延长设备使用寿命。     

In vivo neutralization assays by monoclonal antibodies against white spot syndrome virus in crayfish(Cambarus proclarkii)

The neutralizing activities of eight monoclonal antibodies （MAbs） against white spot syndrome virus （WSSV） （2D2, 2B2,1D2, 1D5, 1C2, 4A1, 6A4 and 6B4） were analyzed by in vivo experiments. Gills from WSSV-infected shrimp were homogenized and ten-fold serially diluted by PBS, and then incubated with MAbs （hybridoma culture supernatant）, respectively. The mixture of WSSV and MAbs were injected into crayfish （Procambarus clarkii）. After challenge, the death rates of crayfish were counted to determine the neutralizing activities of MAbs. At the same time, the mixture of myeloma culture supernatant and WSSV or PBS was served as positive or negative control, respectively. The results showed that, at each virus dilution, the mean time to death of the crayfish injected with MAb-treated virus was significantly longer than that in the positive control, though they all showed 100% mortality within 25 d, and meanwhile, few crayfish died in the negative control. Among the eight MAbs, 2D2, 2B2, 1D2 and 1D5, especially the former two, delayed the mortality significantly, and 1 C2, 4A1 and 6A4 delayed the mortality as well but not so efficiently, while MAb 6IM was efficient only when the virus concentration increased. The results indicated that the anti-WSSV MAbs can neutralize WSSV in different virus dilutions.     

对虾传染性皮下及造血组织坏死病毒衣壳蛋白基因的克隆表达及抗体制备

本文根据GenBank登录的对虾传染性皮下及造血组织坏死病毒(IHHNV)的全基因组序列(NC-002190),设计出主要结构蛋白基因的相应引物,从厦门发病的对虾中,提取DNA,以此为模板,利用PCR扩增目的基因.再将目的基因分别与pGEX-6p1载体和pET-28a载体连接,构建重组表达载体pGEX-Ⅳ、pET-Ⅳ,重组子经酶切和测序鉴定后导入表达型大肠杆菌BL21,IPTG诱导表达,SDS-PAGE检测,大小约为62KDa和41KDa,与预测大小一致.将pET-Ⅳ表达重组蛋白经纯化,免疫小鼠,Western免疫印迹反应结果表明其抗体均可与pGEX-Ⅳ和pET-Ⅳ表达的蛋白发生特异性反应.本研究的结果为对虾IHHNV的快速检测及其免疫提供了研究基础.     

运用荧光显微技术分析北黄海夏季浮游病毒的分布

运用荧光显微技术(Epifluorescence Microscopy,EFM),对2006年夏季北黄海水域31个站点的病毒丰度进行了检测,对浮游病毒丰度在水平分布,垂直分布和昼夜变化上进行了探讨.北黄海水域浮游病毒直接检测量(Virus Direct Count,VDC)为(1.58×106～1.38×107)/mL,平均为5.86×106/mL.在水平分布上,表层、30 m和底层水在辽东半岛头部附近出现了病毒较高区域,近海水域的病毒丰度较中部水域高(P＜0.05).在垂直分布上,表层浮游病毒丰度高于30 m水层和底层(P＜0.05).在昼夜变化上,表层水体中浮游病毒量有明显的变化,10 m水层和30 m水层的病毒丰度昼夜变化较明显,底层病毒丰度昼夜趋势平缓.利用多元相关分析可知,北黄海夏季浮游病毒丰度与站位总水深(P＜0.01)和盐度(P＜0.05)成一定的负相关性,与温度无明显相关性(P＞0.05).     

姬松茸提取物组分体外抗病毒感染活性的研究

为了探明姬松茸提取物的体外抗病毒感染活性及其作用方式,进而为水产养殖鱼类高效抗淋巴囊肿病毒(lymphocystis disease virus,LCDV)感染活性物质的开发和鲆蝶类淋巴囊肿病的防治奠定基础,利用热水浸提和酒精沉淀法得到了5种姬松茸提取物组分(E1～5),并利用MTT、细胞病变程度观察等方法研究了5种组分对LCDV感染体外培养大菱鲆鳍细胞(turbot fin cells,TF细胞)的影响作用.细胞毒性实验结果显示,5种组分对体外培养TF细胞均无毒性.细胞病变程度结果表明,本文所得5种姬松茸提取物组分尤其是E5组分具有显著的抗LCDV感染TF细胞的活性.不同方式感染的实验结果进一步显示,5种姬松茸提取物组分抗LCDV感染TF细胞的作用可能主要是通过直接灭活病毒和/或阻断病毒吸附细胞来实现的.     

筑牢矿业开发“防护墙”——黑龙江省矿产督察员队伍建设纪实

日前,省内两家勘查单位的负责人来到省国土资源厅,补办了矿产资源勘查许可延续手续。他们都是在我省国家级矿产督察员对地质勘查项目进行现场督察时,发现有违规行为而建议整改的。     

Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.