玉米素和水杨酸对雨生红球藻(Haematococcus pluvialis)生长及虾青素积累的影响

虾青素是一种具有强抗氧化活性的类胡萝卜素,而雨生红球藻是天然虾青素的主要来源。本文以雨生红球藻(Haematococcus pluvialis)为材料,研究了植物激素玉米素和水杨酸对雨生红球藻的生长、虾青素含量及相关基因表达的影响。分别添加5种浓度的玉米素或水杨酸,结果发现0.05mg/L玉米素或25mg/L水杨酸处理5d后雨生红球藻虾青素积累最多。该浓度玉米素或水杨酸可显著提高光胁迫下藻细胞密度,最高分别达到3.4×10~5cell/mL和3.0×10~5cell/mL;同时玉米素与水杨酸组中虾青素含量显著上升,分别为1.7%和1.6%,比对照组分别增加29.2%和25.6%。玉米素缓解了高光逆境条件下光合作用基因——Rubisco大亚基(rbcL)及其活化酶(rca)、碳酸酐酶(ca)的下调表达,但对虾青素合成途径β-胡萝卜素酮化酶基因(bkt)的表达量没有显著影响;而水杨酸则相反,在胁迫后期不能缓解光合作用相关基因的下调表达,但可使bkt基因显著上调,最高可达对照组的2.5倍。本研究首次比较了玉米素和水杨酸对雨生红球藻生长和虾青素积累的影响,发现玉米素比水杨酸具有更好的促进雨生红球藻中虾青素积累的效果。     

南海东北部陆架边缘第四纪高分辨率层序地层和沉积体系演化及其控制因素

The northeastern shelf margin of the South China Sea(SCS) is characterized by the development of large scale foresets complexes since Quaternary. Based on integral analysis of the seismic, well logging and paleontological data, successions since ～3.0 Ma can be defined as one composite sequence, consist of a set of regional transgressive to regressive sequences. They can be further divided into six 3 rd order sequences(SQ0–SQ5) based on the Exxon sequence stratigraphic model. Since ～1.6 Ma, five sets of deltaic systems characterized by development of wedge-shaped foresets complexes or clinoforms had been identified. High-resolution seismic data and the thick foresets allowed further divided of sub-depositional sequences(4 th order) of regression to transgression, which is basically consistent with published stacked benthic foram O-isotope records. Depositional systems identified in the study area include deltaic deposits(inner-shelf deltas and shelf-edge deltas), incised valleys, and slope slumping massive deposits. Since ～1.6 Ma, clinoforms prograded from the southern Panyu Lower Uplift toward the northern Baiyun Depression, shelf slope break migrated seaward, whereas the shelf edge of SQ0 migrated landward. The development of incised valleys in the continental shelf increased upward,especially intensive on the SB3 and SB2. The slumping massive deposits increased abruptly since SB2, which corresponds to the development of incised valleys. The evolution of depositional systems of continental slope mainly controlled by the combined influence of sea level changes, tectonic movements, sediment supply and climate changes. Since ～3.0 Ma, relative sea level of the northern SCS had been experienced transgression(～3.0 Ma BP) to regression(～1.6 Ma BP). The regional regression and maximum transgressions of the composite sequences were apparently enhanced by uplift or subsidence related to tectono-thermal events. In addition,climatic variations including monsoon intensification and the mid-Pleistocene transition may have enhanced sediment supply by increasing erosion rate and have an indispensable influence on the development of the incised valleys and 5 sets of deltaic systems since ～1.6 Ma.     

大黄鱼病原副溶血弧菌拮抗菌的筛选

用点种法从大黄鱼肠道优势菌群中筛选出104株病原性副溶血弧菌的拮抗菌,进一步研究拮抗效果最好的11株拮抗菌的抗菌谱和生长曲线,其中NAC平板上筛选的4株拮抗菌的抗菌范围较广,锰营养琼脂培养基上筛选的3株拮抗菌具有生长优势。用BIOLOG板测定了抗菌谱较广、有一定生长优势3株拮抗菌(R22,Y58和J312)的碳源利用情况,结果表明,3株拮抗菌的碳源谱都比较广,都能利用糖类、有机酸和氨基酸等,而且与鱼类营养的竞争较小。16S rRNA基因同源性分析的结果显示与R22,Y58,J312同源性达99%以上的菌株分别来自弧菌属、芽孢杆菌属和假单胞菌属。进一步对R22,J312和Y58三株拮抗菌进行生化鉴定,结果显示R22为溶藻弧菌,J312为荧光假单胞菌,但Y58的种属未能得到确认。所得到的3株拮抗菌R22,Y58,J312在拮抗病原菌、抗菌谱、生长特性和营养利用等方面都初步满足益生菌的筛选条件,有待进一步检测其安全性和实用效果以便能够成为能实用的益生菌。     

凡纳滨对虾(Litopenaeus vannamei)继饥饿后恢复生长期间生化组成及能量收支的动态变化

采用氧弹式热量计和常规的动物生化成分分析方法,测定凡纳滨对虾在恢复生长过程中生化组成和能量分配模式的动态变化。结果表明,饥饿状态下,虾体干重、脂肪含量和能值下降,水分和灰分含量升高,但蛋白质含量没有明显改变。恢复投喂后,能量特定生长率显著提高,而虾体各生化组成在不同时间内均恢复到对照组水平。饥饿使对虾恢复生长过程特别是其前期的能量分配模式发生极显著变化。对虾的摄食能分配于生长的比例增大,而损失于代谢消耗的比例则相应减少;摄食能的增加和消化吸收率提高则仅发生在恢复生长的初期。可以认为,凡纳滨对虾继饥饿后的补偿生长效应主要出现在恢复生长的前期,并随时间延长逐步减弱,其生理机制最主要是得益于代谢耗能比例的减少。     

浅海和陆相地层的高分辨率层序地层对比——以东犹他州书崖地区白垩系黑鹰组Sunnyside段为例

现代地层学研究中一个关键的问题是陆相和浅海相地层的对比和整和,尽管层序地层学在两种地层中均有很大发展,可是很少有成功的两者对比研究,本文的目的就是用陆地有机相和海相地层水进水退来产生一种新的层序地层演化的整和模式。     

利用父母本微卫星标记估计大菱鲆(Scophthalmus maximus L.)幼鱼生长性状的遗传参数

The estimation of genetic parameters has played an important role in animal selective breeding for growth traits.Recently studies show that molecular markers can be incorporated into genetic evaluations. In order to improve the performance of an incomplete pedigree(i.e, only parents are known) in the genetic evaluations, 12 microsatellite markers have been applied in the estimation of the genetic parameters for body weight in a farmed population(n=1 890) of juvenile turbot(Scophthalmus maximus L.). A new relatedness called parental molecular relatedness(PMR) is estimated based on results of genotyping of 48 parents(31 males, 17 females) with microsatellites markers. The feasibility of PMR in estimation of genetic parameters is verified by comparison with pedigree related(PR) which is obtained from a complete pedigree. The results demonstrate that a high correlation(0.872) between them is found. Heritabilities are estimated using the PMR(0.52±0.13) and PR(0.55±0.22) with the same animal model. A cross-validation shows that the predictive abilities of models using the PMR and the PR are identical(0.81). From that, a conclusion can be made that PMR and PR predicted genetic values equally well in a population of juvenile turbot. Therefore PMR can be applied as an alternative of the PR when only parents are known. However, for a better performance, more markers and more families should be included in a further study.     

营养限制对三种浮游动物的影响

Nutrient imbalance—a mismatch in nutrient ratios between the available food supply and the demands of consumers—has the potential to be transported up food chains,exposing zooplankton to nutrient limitations.In this study,the response of Calanus sinicus(copepod),Moina mongolica(cladocera),and Brachionus plicatilis(rotifer) to nutrient-limited(no-limited,P-limited,and N-limited) food were evaluated from the perspective of growth,reproduction,and stoichiometric homoeostasis.The results indicated that the growth of three species was suppressed under nutrient-limited(especially P-limited) conditions.However,the effect of nutrient limitations on their reproduction was species-specific.For C.sinicus,the dietary UFA(unsaturated fatty acid) as a major phospholipid component affected their egg production and total FA supporting energy promoted the hatchability of eggs.Furthermore,excess carbon in the diet promoted egg production but reduced hatching success.For M.mongolica,nutritional(P and UFA) and energy(total FA) support affected their fecundity.B.plicatilis fecundity exhibited the same pattern of growth(no-limitedN-limitedP-limited).In terms of stoichiometric homeostasis,B.plicatilis' s elemental compositions were less likely to be affected by nutrient limitations than M.mongolica.We suggest that the effects of nutrient imbalances could potentially become an evolutionary force affecting ecosystem structure and stability in eutrophic waters.     

济阳坳陷博兴洼陷西部沙三段层序地层

选取以基准面为参照面的高分辨率层序地层学的理论与分析技术,对博兴洼陷西部沙三段开展层序地层分析工作。在博兴洼陷沙三段识别出5个层序界面和4个较大规模的洪泛面,由此将研究层段划分为4个长期基准面旋回(相当于3级层序),并通过长期旋回内部次级转换面的识别,细分出8个中期旋回(大致相当于4级层序)。通过对比建立了研究区的高分辨率层序地层格架,并分析了各层序的地层发育特征。以层序格架为基础,探讨了研究区各层序的沉积演化特征,建立了辫状三角洲—浊积扇层序发育模式,认为研究区辫状三角洲和浊积扇均具有加积作用特点;斜坡区为辫状三角洲发育区,而洼陷区为浊积扇发育区;中期基准面旋回下降期辫状三角洲发育,上升期浊积扇发育;浊积扇体的发育规模与湖泛规模相关。综合分析认为,浊积扇是形成岩性圈闭的主要储集砂体类型,其发育的有利层位是MSC8、MSC7、MSC6、MSC5旋回的上升半旋回,岩性圈闭发育的有利区是博兴南部斜坡坡折带之下的洼陷区。     

大川中地区须家河组二、四段地震相与沉积相研究

四川盆地上三叠统须家河组油气勘探开始于20世纪40年代,其中须二段、须四段是主要探目的层段,也是盆地周边地区寻找新气源最有利层位之一。依据地震地层学方法和理论,结合钻井与测井资料,将大川中地区须家河组划分成7个地震层序边界,分别为SB1—SB7,与之对应6个最大湖泛面,分别为MFS1—MFS6,并对层序界面的地震反射特征进行了总结。利用地震反射内部结构、外部形态和反射波终止类型,在大川中地区须家河组须二段与四段内共识别出平行—亚平行、前积、丘状、透镜状及发散状地震反射特征等5种地震相,并对18条二维地震测线进行了剖面地震相的综合解释,编制了须二段与四段的地震相平面分布图。通过地震相与沉积相的转换,分别划分出冲积扇、扇三角洲、辫状河三角洲以及湖泊等沉积体系,编制了大川中地区须家河组二段与四段沉积相平面图,对大川中地区须家河组构造格局的演化规律进行了分析。     

棉粕蛋白酶解物对异育银鲫(Carassius auratus gibelio)消化、生长和胰蛋白酶mRNA表达量的影响

以棉粕蛋白为酶解底物,用枯草杆菌蛋白酶对其进行酶解,以酶解产物1.5%和3.0%两个梯度等量替代鱼饲料配方中棉粕,在室内流水养殖系统中喂养异育银鲫鱼种[体重为(30±2)g]65天。测定鱼的生长、营养物质表观消化率、消化蛋白酶活性及肝胰脏中胰蛋白酶mRNA表达水平等指标。结果表明,添加1.5%和3.0%棉粕酶解产物的鱼在饲养35天后的特定增长率(SGR)分别比对照组高32.5%和56.7%,且差异显著(P<0.05);在饲养65天后,两组特定增长率分别比对照组高8.0%和21.0%,且差异极显著(P<0.01),肝胰脏中胰蛋白酶mRNA表达水平也随棉粕酶解产物添加梯度提高而相应提高,表明鱼的生长与消化蛋白酶mRNA表达水平相关。同时棉粕蛋白酶解物对肠道蛋白酶的活性和营养物质表观消化率都有促进作用,而棉粕蛋白酶解物对鱼肌肉成分并没有改变,这也表明棉粕蛋白酶解物在促进鱼生长、内源酶活性同时并未降低鱼的品质。