香港牡蛎(Crassostrea hongkongensis)新型凝集素ChPerlucin的基因克隆与功能研究

C-型凝集素(C-type lectin)是一类能识别并结合多糖的免疫蛋白质超家族, 本文首次克隆了一种新型香港牡蛎C-型凝集素基因——甘露聚糖结合凝集素, 并获得了该基因的全长序列, 命名为ChPerlucin。结果显示, ChPerlucin基因cDNA全长577bp, 包括21bp的5′末端非翻译区(untranslated region, UTR)、73bp的 3′UTR, 以及483bp的开放阅读框(open reading frame, ORF), 编码160个氨基酸, ChPerlucin蛋白理论分子量为18kD, 等电点为5.95。ChPerlucin蛋白含有保守的C-型凝集素样结构域(C-type lectin or carbohydrate-recognition domain, CLECT); 邻接法(Neighbor-Joining, NJ)进化树分析表明, ChPerlucin与其他贝类的Perlucin聚为一支, 说明该基因是软体动物Perlucin家族的新成员。qRT-PCR结果显示, ChPerlucin在香港牡蛎所检测组织和各个胚胎发育时期中均有表达; 病原菌刺激后, ChPerlucin表达量显著升高; 利用毕赤酵母真核成功表达了ChPerlucin重组蛋白, 发现ChPerlucin重组蛋白具有抑菌效果。以上结果表明ChPerlucin在香港牡蛎的先天性免疫过程中发挥了重要的作用。     

海水酸化对鹿角珊瑚光谱特性影响的分析

海水酸化造成全球珊瑚礁严重退化,应用卫星遥感手段可以快速地对珊瑚礁进行监测。在野外做酸度对比实验具有条件不易控制、周期长等局限性。文章提出一种室内测量珊瑚光谱的方法,通过比较不同酸度梯度下珊瑚光谱的变化,为研究海水酸化对珊瑚的影响提供了一种新的思路。试验采用7.6、7.9和8.1的酸度梯度,结果表明:在pH为8.1和7.9环境条件下,珊瑚的光谱趋势大体一致,总体上珊瑚光谱波峰出现了向长波方向的红移。但是在pH为7.6的条件下,珊瑚的光谱在650~700nm之间出现一个反常的吸收谷,这是由于pH 7.6的酸度条件适宜一些藻类生长,藻类附着在珊瑚表面,从而影响了其光谱特性。     

Effects of an artificial oyster shell reef on macrobenthic communities in Rongcheng Bay,East China

An artificial oyster shell reef was deployed in Rongcheng Bay, East China. However, the effects of this reef on the surrounding macrobenthic communities were unknown. We compared sedimentary factors, macrobenthic biomass, abundance, and community composition and ecological indicators between the reef and non-reef areas over a one year period. The mean values for chlorophyll a （Chl a）, total organic matter （TOM）, total organic carbon （TOC）, and total nitrogen （TN） content in surface sediments in the reef area were slightly higher than those in the non-reef area. The Chl a levels differed significantly between the two areas, but the TOM, TOC, and TN were not significantly different. The abundance of crustaceans was significantly different between the two areas, but the abundance and biomass ofpolychaetes, echinoderms, mollusk did not differ significantly. The permutational multivariate analysis of variance （PERMANOVA） revealed that the macrobenthic community differed significantly through time and analysis of similarity multivariate analyses （ANOSIM） revealed that the macrobenthic community differed significantly in some months. The ecological indicators revealed that the environmental quality of the reef area was slightly better than that of the non-reef area. Overall, our results suggest that the artificial oyster shell reef may change the macrobenthic community and the quality of the environment. Despite the lack of an effect in the short term, long-term monitoring is still needed to evaluate the effects of artificial oyster shell reefs on macrobenthic communities.     

The potential of ocean acidification on suppressing larval development in the Pacific oyster Crassostrea gigas and blood cockle Arca inflata Reeve

We evaluated the effect of pH on larval development in larval Pacific oyster （Crassostrea gigas） and blood cockle （Arca inflata Reeve）. The larvae were reared at pH 8.2 （control）, 7.9, 7.6, or 7.3 beginning 30 min or 24 h post fertilization. Exposure to lower pH during early embryonic development inhibited larval shell formation in both species. Compared with the control, larvae took longer to reach the D-veliger stage when reared under pH 7.6 and 7.3. Exposure to lower pH immediately after fertilization resulted in significantly delayed shell formation in the Pacific oyster larvae at pH 7.3 and blood cockle larvae at pH 7.6 and 7.3. However, when exposure was delayed until 24 h post fertilization, shell formation was only inhibited in blood cockle larvae reared at pH 7.3. Thus, the early embryonic stages were more sensitive to acidified conditions. Our results suggest that ocean acidification will have an adverse effect on embryonic development in bivalves. Although the effects appear subtle, they may accumulate and lead to subsequent issues during later larval development.     

珠江口盆地东沙隆起生物礁地质及地震特征

东沙隆起珠江组生物礁是珠江口盆地重要的储层类型,礁灰岩主要为珊瑚藻灰岩,分为骨架岩、粘结岩及障积岩。研究区生物礁发育于开阔台地、台地边缘及台地前缘斜坡3类沉积相中,生物礁类型可分为台内点礁、台地边缘堡礁及台缘斜坡塔礁,以台地边缘堡礁为主。珠江组沉积早期,东沙隆起发育滨岸砂质沉积;中期海水逐渐淹没东沙隆起,形成镶边浅水碳酸盐台地,发育生物礁;晚期海侵台地逐渐淹没消亡,被浅海陆棚泥质沉积代替。流花台地厚度最大,最厚达563m,台地顶面地震强反射使外形轮廓清晰,台地内部中-弱振幅至空白反射,连续性降低;惠州台地厚度较流花台地薄,厚度多大于100m,顶界面强反射轮廓清晰,内部层状反射能量强-中振幅、高-中连续、中-低频,局部空白反射;陆丰台地厚约30m,只有一个高连续的强振幅。生物礁地震响应以丘形、箱型、低丘-透镜状为特征,礁顶常为强振幅,内部为波状、有时见前积结构,翼部可见上超和披覆现象等。     

六盘山地区下白垩统六盘山群李洼峡组叠层石特征及成因环境探讨

在六盘山地区下白垩统六盘山李洼峡组中发育了湖相丘状叠层石,通过对叠层石的特征和叠层石地球化学组分分析表明,研究区叠层石主要产于安静低能水体环境,而且对沉积物的捕捉和粘附作用不甚明显,生物膜的钙化和原地微生物引发的沉淀作用是主要成因。经分析,研究区叠层石的Sr/Ba值为12.36,CaO含量为45.56%,总体上,研究区叠层石形成于盐度高且温度较高、气候炎热、蒸发量大而且较为干旱的环境。     

塔里木盆地西北缘柯坪地区油气勘探前景再认识

柯坪地区地面油苗分布层系多、范围广,发育寒武系、奥陶系、石炭系、二叠系多套烃源岩,该区经历了大规模油气成藏过程和强烈的破坏作用,油气勘探最关键的风险因素是保存条件。从保存的角度看,寒武系膏盐岩是一套优质盖层,膏盐层与盐间及盐下碳酸盐岩、玉尔吐斯组烃源岩构成良好的生储盖组合。青松采石场奥陶系轻质油流揭示现今可能有碳酸盐岩残留油气藏存在,美国中部地台区辛辛那提隆起奥陶系浅层油藏就是例证。地面油苗中,石炭-二叠系碳酸盐岩中的出露点最多,且礁灰岩储集性能好,在埋藏区可能得到保存。基于地面调查难以进一步深化认识和推动勘探,建议评价选择地质目标实施钻探。目前经济技术条件下比较现实的目标为:萨拉姆布拉克背斜构造,落实寒武系膏盐岩及玉尔吐斯组烃源岩,了解盐间及盐下含油气性;青松采石场奥陶系碳酸盐岩残留油气藏分布区;皮羌村西南哈拉峻盆地石炭-二叠系礁灰岩目的层。     

牡蛎的遗传育种研究进展

牡蛎肉味鲜美，营养丰富，是世界重要海水贝类养殖对象之一。随着各种生物技术在水产领域的广泛应用，牡蛎的遗传育种工作也备受重视，尤其自Stanley首次人工诱导美洲牡蛎(Crassostrea virginica)三倍体成功以来，大大加快了对牡蛎的遗传学背景和育种进程的研究步     

西沙石岛生物礁的矿物组成及其环境指示意义

通过对西科1井岩心碳酸盐岩样品进行矿物组成分析, 来研究礁体的发育过程和古海洋环境的变化.初步研究结果表明: 西科1井岩心碳酸盐岩的矿物由低镁方解石、高镁方解石、文石和白云石组成.矿物组成特征表明, 井深35.4 m处为一重要的地层界面或环境变化界面.在岩心(井深0~748 m)中共识别出5个白云岩层, 其中上新统莺歌海组一层(井深: 289.3~312.3 m, 厚约23 m), 上中新统黄流组三层(分别位于井深: 373.3~412.7 m, 厚约39.4 m; 424.7~450.6 m, 厚约26.1 m; 469.70~564.96 m, 厚约95.2 m), 中中新统梅山组一层(井深: 615.20~636.96 m, 厚约21.7 m).各层的白云岩化程度不尽相同, 5层白云岩中白云石矿物含量的最大值在79.3%~100%之间.生物礁的发育主要受控于古海洋环境的变化, 与古气候变化有关的海平面升降间接地控制了岛礁碳酸盐岩的白云岩化作用.石岛西科1井中的5层白云岩应该是在较长时间稳定环境条件下发育而成, 主要应该是蒸发泻湖渗透回流作用下早期形成的方解石类碳酸岩白云岩化的结果, 但并不排除混合水白云岩化作用的可能性.      

羌塘盆地半岛湖地区上侏罗—下白垩统礁灰岩地球化学特征和成岩流体性质

半岛湖地区生物礁以发育灰岩为主,包括以造礁生物为主的骨架岩和非礁碳酸盐岩,针对生物礁相碳酸盐岩的显微组构分析和阴极发光分析,测得不同点礁各类礁相碳酸盐岩的常微量元素和碳、氧同位素组成,分别为:35件样品Mn,Sr,Fe元素平均质量分数为129×10-6,270×10-6和1 320×10-6,具有较低的Mn含量、较高的Sr含量、Fe含量,表明在成岩过程中海源流体发挥了主导作用,同时遭受了不同程度的大气淡水成岩流体的影响;碳同位素值变化不大且都为正值,变化区间为1.4‰~3.5‰,平均值为2.61‰;氧同位素值变化区间为-7.4‰~-2.7‰,平均值为-5.25‰,古海水盐度指数Z值变化较小,为126.6~132.7,平均值为130.5。结果表明,成岩流体盐度较高,溶蚀孔洞较为发育的颗粒灰岩和礁灰岩具有相对更低的δ18 O值,晚侏罗—早白垩世时期,羌塘盆地北部大量有机碳被高速埋藏是导致半岛湖地区礁相碳酸盐岩碳同位素值普遍偏高的首要原因。