Oyster reef is an important component of blue carbon sink, which completes carbon sequestration through calcification, biological assimilation, and biological deposition, by which carbon neutralization is achieved. Currently, research on the carbon storage capacity of tropical natural oyster reefs in China is limited. The carbon pool of four typical oyster reefs (Longlou in Wenchang (LW), Huiwen in Wenchang (HW), Changhua in Changjiang (CC), and Haitou in Danzhou (HD)) in Hainan Island was evaluated based on the oyster samples collected from June 2022 to February 2023, and the characteristics of temporal and spatial variation and their causes were analyzed. Results show that the total existing carbon pool storage of the four reef areas were 18 734.6 t C and the total long-term carbon pool reserves were 16 883.08 t C. The existing carbon pool reserves (average carbon pool reserves) in HD, CC, LW, and HW were 15 923.42 t C (3.46 kg C/m2), 2 119.01 t C (6.09 kg C /m2), 494.53 t C (2.49 kg C /m2), and 197.63 t C (3.67 kg C /m2), respectively, and the long-term carbon pool reserves are 14 334.11, 1 898.99, 460.08 and 189.91 t C, respectively. The carbon storage per unit area of HD and CC oyster reefs might be affected by high or low temperature, and the carbon storage per unit area decreased in July and December. However, in October and February, when water temperature was suitable, the oyster grew rapidly, leading to the increase in carbon storage per unit area. It is worth noting that the reproduction of oysters may be another important factor for the increase of carbon storage per unit area in October. From June to December 2022, the loss of oyster shells in LW area led to the continuous decrease of carbon storage per unit area of oyster reef, while the mortality rate of oysters in HW area increased in July, October 2022, and February 2023, resulting in a decrease in carbon fixation capacity of living shell and soft part of oysters decreased. Result shows that the carbon fixation efficiency of natural oyster reef in Hainan Island was relatively high, and its carbon storage was greatly affected by temperature, sea conditions, and surrounding aquaculture activities. These areas are still facing the risk of anthropogenic disturbance and system degradation, calling for the urgent need for relevant protection policies and effective artificial proliferation to ensure their good carbon sequestration function. 相似文献