一株深海耐嗜压菌的细胞毒活性次生代谢产物研究

通过CCK8法检测分离自深海一株深海耐压菌Shewanella piezotolerans WP3次生代谢产物的细胞毒活性,研究了温度、压力、噬菌体对WP3次生代谢产物细胞毒活性的影响.发现WP3次生代谢产物具有良好细胞毒活性.低温因素可提高其细胞毒活性,在100μg/cm^3的含量下,代谢产物对肿瘤细胞Bel7402、CNE2、A549、HT1080细胞毒活性均有提高,其中对A549细胞毒活性提高了1.3倍.在最适温度（20℃）情况下,高压可降低其细胞毒活性.20 MPa培养条件下,代谢产物对4株肿瘤细胞均未表现出明显活性,而常压培养条件下对SW 480具有93.3%的抑制率,对HT1080抑制率表现出87.5%,对CNE2抑制率是79.8%.在低温情况下,压力因素对其细胞毒活性无影响,噬菌体对细胞毒活性影响不大.HPLC指纹图谱分析发现不同温度条件下,其次生代谢产物指纹图谱有变化,结合细胞毒活性结果分析,为后期分离鉴定细胞毒活性化合物提供指导.     

预修正快速傅里叶变换方法在波浪绕射和辐射问题中应用

在波浪对海上建筑物绕射和辐射问题的计算中,面元法被广泛使用,但由于传统面元法的存储量和计算量均为未知量的平方量级,很难满足大范围多未知量问题的计算需要。采用预修正快速傅里叶变换方法(pFFT方法),使计算量与存储量都降低到未知量的线性量级。以淹没圆球与漂浮圆柱两个典型算例为基础,通过不同未知量时pFFT方法与传统面元法的计算量与存储量的对比,以及pFFT方法自身各步骤计算时间的对比,研究了不同网格方案的选取对pFFT方法计算量和存储量的影响,推荐根据未知量个数采用计算时间最小化原则选取pFFT网格参数。     

南黄海中部泥质沉积成因和物源研究综述

当前南黄海沉积学研究的核心问题是泥质沉积的形成过程和动力机制及其物质来源。许多学者对南黄海中部泥区的成因和物源进行了大量研究,但比较局限,缺少系统性。现有研究认为南黄海泥质沉积的形成受黄海冷水团和上升流的影响,但黄海冷水团并不是其形成的必要条件。对泥区物源的研究,主要考虑现代黄河物质、老黄河物质以及长江物质对其的影响,并且仅是定性的研究。对于朝鲜半岛物质对南黄海中部泥区的影响,国内的研究更是寥寥无几。海水对海底基岩的侵蚀沉积以及黄海暖流对海底沉积物的改造和再悬浮作用对南黄海中部泥区沉积的贡献仅是个别学者的观点。     

腾格里沙漠东南缘人工固沙植被区表层土壤有机碳矿化对凋落物添加的响应

土壤有机碳矿化是调控土壤碳库时空格局、土壤碳收支平衡和植物养分供应的重要过程,植物残体和凋落物分解释放CO₂直接影响着土壤有机碳矿化。研究了不同类型凋落物对腾格里沙漠东南缘建植于1956年的人工固沙植被区土壤有机碳矿化过程及其对水分和温度的响应特征。结果表明：凋落物添加显著促进了有机碳矿化,添加柠条锦鸡儿（Caragana korshinskii）、油蒿（Artemisia ordosica）、小画眉草（Eragrostis minor）凋落物后,CO₂-C最大矿化速率分别增大了6.94、5.17、3.46倍,0~5 cm层土壤是5~10 cm层土壤的1.09、1.55、1.22倍;CO₂-C累积释放量分别增加了3.73、3.38、2.34倍,0~5 cm层土壤是5~10 cm层土壤的1.17、1.30、1.57倍。凋落物对有机碳矿化的促进作用与温度和水分密切相关,25℃时,CO₂-C平均释放速率、最大释放速率、累积碳释放量分别是10℃的2.21、3.60、2.21倍,而含水量10%时,CO₂-C平均释放速率、最大释放速率和累积碳释放量分别是含水量5%时的1.25、1.20、1.25倍。相关性分析表明,凋落物碳氮含量、碳氮比、木质素比氮和土壤有机碳以及全氮是影响有机碳矿化的主要因子。凋落添加土壤后潜在可矿化碳表现为柠条锦鸡儿>油蒿>小画眉草>对照。凋落物添加显著促进了有机碳矿化过程及碳周转,植被恢复过程中草本植物凋落物的输入更有利于土壤碳固存,凋落物对土壤碳库的调控作用受土壤理化性质和水热等环境因子的共同作用影响。     

洛阳市:飓风行动 彻底整改

2008年11月23日,一场声势浩大的集中拆除违法建（构）筑物的“飓风行动”在洛阳全面展开。截至12月25日,洛阳市135宗违法用地全部整改到位,违法建筑拆除率100％。这次“飓风行动”共拆除违法建筑物296714平方米,复耕土地420余亩,收取罚没款1984．03万元,移送司法机关21人,追究刑事责任4人,受党政纪处分21人,其中处级干部1人,科级干部20人。     

BTEX anomalies used as indicators of submarine oil and gas reservoirs

It is a conventional method for petroleum prospecting to generally use paraffin hydrocarbon as basic indexes of oil and gas. This conventional geochemical technology, however, shows some limits in the prospecting as paraffin is vulnerable to influences from human and biologic activities. Consequently, BTEX (short for benzene, toluene, ethyl benzene and xylem, which are direct biomarkers) among aromatic hydrocarbon series has been taken into account for the oil and gas prediction. Domestic and foreign study results demonstrate that BTEX is hardly disturbed and can well indicate oil and gas reservoirs. Based on measured data from a South China Sea area, the present authors have used self-developed visual assessment software for petroleum prospecting has been used to process data, strip background anomalies, and outline significant BTEX anomalies. By comparison with stratigraphic profiles of the target area, it is confirmed that BTEX is a good indication of marine oil and gas during the petroleum prospecting.     

壳聚糖硫酸酯金属配合物的抗氧化活性

以吩嗪硫酸甲酯-NADH为超氧阴离子自由基(O_2~(·-))产生、检测体系和EDTANa_2-Fe(Ⅱ)-H_2O_2为羟自由基(~·OH)产生、检测体系,对壳聚糖硫酸酯铜、锌配合物和不同分子质量壳聚糖进行了抗O_2~(·-)和~·OH自由基的活性研究.结果表明,壳聚糖硫酸酯金属配合物对于O_2~(·-)自由基的清除能力明显高于壳聚糖,在质量浓度为0.025 g/L时,壳聚糖硫酸酯铜配合物对O_2~(·-)自由基的清除能力达到94.18%,壳聚糖硫酸酯锌配合物达到93.19%;壳聚糖硫酸酯铜、锌配合物对~·OH自由基的清除能力(67.39%、60.46%)低于相同分子质量的壳聚糖(88.06%),而高于高分子质量壳聚糖761 ku(18.71%);壳聚糖分子质量大小对O_2~(·-)和~·OH自由基的清除能力有较大影响,质量浓度为1.6 g/L壳聚糖分子质量为20 ku时,对O_2~(·-)清除率达54.69%,而分子质量在761 ku时,对O_2~(·-)清除率仅为35.50%;各样品对O_2~(·-)和~·OH自由基的清除能力均随着质量浓度的增加而上升,壳聚糖硫酸酯铜、锌配合物在相当低的浓度下(0.025 g/L)就可以达到明显清除O_2~(·-)自由基的效果(≥90%).     

涠洲岛潮滩表层沉积物中重金属的空间分布及其环境质量评价

根据2006年4月对涠洲岛潮滩表层沉积物的调查资料,分析了表层沉积物中重金属元素Hg、Cu、Pb、Zn、Cd、Cr、As的含量水平及空间分布特征,运用单因子评价法对其环境质量进行了评估,并通过相关性分析、因子分析探讨了几种重金属元素的物源.结果表明:涠洲岛潮滩环境质量状况良好,各元素均未达到污染水平,多数地区存在Cu的玷污,个别地区存在As的玷污;各分析元素较多的累积在人为活动相对频繁的XKZ、W05断面附近区域;Hg、Cu、Zn、Cr显著正相关,以环境背景来源为主,Pb、As较多的受到人为因素的影响.     

Impacts of soil fauna on litter decomposition at different succession stages of wetland in Sanjiang Plain, China

Litter decomposition is the key process in nutrient recycling and energy flow. The present study examined the impacts of soil fauna on decomposition rates and nutrient fluxes at three succession stages of wetland in the Sanjiang Plain, China using different mesh litterbags. The results show that in each succession stage of wetland, soil fauna can obviously increase litter decomposition rates. The average contribution of whole soil fauna to litter mass loss was 35.35%. The more complex the soil fauna group, the more significant the role of soil fauna. The average loss of three types of litter in the 4mm mesh litterbags was 0.3–4.1 times that in 0.058mm ones. The decomposition function of soil fauna to litter mass changed with the wetland succession. The average contribution of soil fauna to litter loss firstly decreased from 34.96% (Carex lasiocapa) to 32.94% (Carex meyeriana), then increased to 38.16% (Calamagrostics angustifolia). The contributions of soil fauna to litter decomposition rates vary according to the litter substrata, soil fauna communities and seasons. Significant effects were respectively found in August and July on C. angustifolia and C. lasiocapa, while in June and August on C. meyeriana. Total carbon (TC), total nitrogen (TN) and total phosphorus (TP) contents and the C/N and C/P ratios of decaying litter can be influenced by soil fauna. At different wetland succession stages, the effects of soil fauna on nutrient elements also differ greatly, which shows the significant difference of influencing element types and degrees. Soil fauna communities strongly influenced the TC and TP concentrations of C. meyeriana litter, and TP content of C. lasiocapa. Our results indicate that soil fauna have important effects on litter decomposition and this influence will vary with the wetland succession and seasonal variation. Foundation item: Under the auspices of State Key Development Program for Basic Research of China (No. 2009CB421103), Key Program of National Natural Science Foundation of China (No. 40830535/D0101), Knowledge Innovation Programs of Chinese Academy of Sciences (No. KZCX2-YW-BR-16, KSCX2-YW-N-46-06)     

Application of matter-element model in soil nutrient evaluation of ecological fragile region

On the basis of the soil environment investigation in Da'an City, Jilin Province, China, 40 soil samples from main land use types were obtained and tested by standard method. Soil organic matter (SOM), total N (TN), total P (TP), total K (TK), available N (AN), available P (AP) and available K (AK) were chosen as the evaluation factors. A regional soil nutrient evaluation model was developed based on the matter-element model. The results show that the soil samples with nutrient grade Ⅱ-Ⅴ respectively account for 10%, 30%, 32.5% and 27.5%, and those with grade Ⅳ and Ⅴ account for 60% in all samples. The relationship between soil nutrients and land types indicates that the nutrients of farmland are relatively good, with 41.7% of soil samples with the nutrient grade Ⅳ and Ⅴ. The nutrients of saline-alkali land and sandy land are the worst, with 100% of soil samples with the nutrient grade Ⅳ and Ⅴ. And the ratios of soil samples grade Ⅳ and Ⅴ in grassland and wasteland are respectively 62.5 % and 54.55%. Generally speaking, the soil nutrients status in Da'an City is poor, 60% of soil samples are in poor and extremely poor conditions, indicating that the soil has been severely eroded. Being a relatively superior evaluation method with more accurate resuits and spatial distribution consistency, matter-element analysis is more suitable for regional soil nutrient evaluation than previous models.