Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.     

湖南地质找矿工具史（上）

[编者按]2009年3月27日,国土资源部颁发<关于印发开展地质找矿改革发展大讨论工作方案的通知>,决定集中半年时间,广泛深入地开展多层面、多领域的地质找矿改革发展大讨论.这是对日渐紧张以及某些稀有资源被大肆而无序地开采导致枯竭的一种拯救,也是对人类可持续发展的一种探索,同时,对构建地质找矿的新机制的形成也是有力的支持.     

非法转让划拨土地使用权处罚时效如何计算

[案情] 2000年,某市国土资源局将一宗国有建设用地使用权依法划拨给某单位建办公综合楼,而该单位却在2003年将该宗地非法转让给某房地产公司搞房地产开发.直至2007年购房者办理土地使用证时,市国土资源局才发现该宗地已非法转让.     

Nonlinear numerical simulation on extreme-wave kinematics

A fully nonlinear numerical model based on a time-domain higher-order boundary element method (HOBEM) is founded to simulate the kinematics of extreme waves. In the model, the fully nonlinear free surface boundary conditions are satisfied and a semi-mixed Euler-Lagrange method is used to track free surface; a fourth-order Runga-Kutta technique is adopted to refresh the wave elevation and velocity potential on the free surface at each time step; an image Green function is used in the numerical wave tank so that the integrations on the lateral surfaces and bottom are excluded. The extreme waves are generated by the method of wave focusing. The physical experiments are carried out in a wave flume. On the horizontal velocity of the measured point, numerical solutions agree well with experimental results. The characteristics of the nonlinear extreme-wave kinematics and the velocity distribution are studied here.     

The mapping methods and division of tectonic units of the regional tectonic map in the eastern China seas and adjacent regions

The geological-geophysical map series of the eastern China seas and adjacent region (1:1 000 000) will be published in the late half year of 2009. The regional tectonic map is one of the main professional maps. The Mapping methods, the division method of geological tectonic units and the main geological tectonic units are mainly discussed. The strata from Pliocene to Holocene are peeled off so as to display the Pre-Pliocene structures. In basins, isopaches are drawn for the Cenozoic deposits. The plate tectonic theory and present tectonic pattern are adopted as the priorities in tectonic division. As to the division of intraplate tectonic units, it is a revision, complement and improvement of previous dividing systems, and the nomenclature for each tectonic unit follows the current system in China. The first-order tectonic unit is plate (Pacific Plate, Eurasian Plate and Philippine Sea Plate). The second-order tectonic unit is tectonic domain (East Asian continental tectonic domain,East Asian continental margin tectonic domain and west Pacific tectonic domain). The Philippine Sea Plate and the west part of the Pacific Plate are called the West Pacific tectonic domain. The part of the Eurasian Plate involved in this study area can be further divided into East Asian continental tectonic domain and East Asian continental margin tectonic domain. The East Asian continental margin domain is composed of the Ryukyu island arc, the Okinawa Trough back-arc basin and the back-arc basin of Sea of Japan. The East Asian continental tectonic domain in this study area is composed of the Sino-Korea Massif, the Changjiang River (Yangtze) Massif and South China Massif. In turn, these massifs consist of basins, folded belts or uplift zones. The basins,the folded belts or the uplift zones are further divided into uplifts and depressions made up of sags and swells.     

A Global Reliability Assessment Method on Aging Offshore Platforms with Corrosion and Cracks

Corrosion and fatigue cracks are major threats to the structural integrity of aging offshore platforms. For the rational estimation of the safety levels of aging platforms, a global reliability assessment approach for aging offshore platforms with corrosion and fatigue cracks is presented in this paper. The base shear capacity is taken as the global ultimate strength of the offshore platforms. It is modeled as a random process that decreases with time in the presence of corrosion and fatigue crack propagati...     

Numerical Prediction of Induced Pressure and Lift of the Planing Surfaces

This paper discusses the numerical prediction of the induced pressure and lift of the planing surfaces in a steady motion based on the potential flow solver as well as the spray drag by use of the practical method.The numerical method for computation of the induced pressure and lift is potential-based boundary element method.Special technique is identified to present upwash geometry and to determine the spray drag.Numerical results of a planing flat plate and planing craft model 4666 are presented.It is shown that the method is robust and efficient and the results agree well with the experimental measurements with various Froude humors.