细基江蓠繁枝变型对水体硝态氮变化的生理响应

通过静态模拟实验,比较研究了6个硝态氮(NO3--N)摩尔浓度水平(0,10,20,40,60,80μmol/L)下培养的细基江蓠繁枝变型(Gracilariatenuistipitatavar.liuiZhangetXia)的丙二醛(MDA)、游离脯氨酸含量以及硝酸还原酶(NR)、超氧化物酶歧化酶(SOD)、过氧化物酶(POD)活性的变化。结果表明,随着水体NO3--N摩尔浓度的逐渐增加,细基江蓠繁枝变型NR、SOD和POD的活性均呈现先增加后下降的显著变化(P<0.05),膜脂过氧化产物MDA含量随水体NO3--N摩尔浓度的变化则呈相反的趋势(P<0.05);游离脯氨酸含量则与水体NO3--N摩尔浓度呈正相关(r=0.9161)。综合分析结果认为,单独以硝态氮为氮源时,20μmol/L的供氮水平能使细基江蓠繁枝变型处于相对最佳的生理状态。     

陕西南郑地区第一口参数井钻获牛蹄塘组页岩气

1 研究目的(Objective) 研究区地处陕西省南郑县南部,构造位置位于华南板块扬子陆块西北缘,具体属于汉南隆起、米仓山隆起之间的大竹坝—回军坝复向斜,区内地层总体发育较全,以古生界和中生界为主.研究区内构造变形较强,是矿权空白区,前期基础地质调查表明该区具有一定的页岩气资源潜力.本次研究的目的是查明该地区主要目的...     

Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。     

A new pelagic Polyconchoecia Xiang, Chen and Du, 2018 (Ostracoda: Myodocopa: Halocyprididae) from the South China Sea

Halocyprid ostracods are appreciable part of ostracods floating through virtually everywhere in marine environment.In this study,we describe a new species of genus Polyconchoecia Xiang,Chen and Du,2018,tribe Conchoeciini Chavtur and Angel,2011,family Halocyprididae Dana,1853 from the middle of the South China Sea.Polyconchoecia chenii sp.nov.is very close to P.commixtus Xiang,Chen and Du,2018.But it differs from P.commixtus by the distinctions of locations of major glands of carapace and the characteristics of appendages:more posteriorly situated left asymmetric gland of carapace,no right asymmetric gland;segmented frontal organ;the endopod 2 of the first antenna with a very small seta;a-and c-setae of the first antenna with long end joint have long end joint,the b-and d-setae have no end joint,spinose e-seta without end joint;the e-seta of the second antenna is present;teeth side is distinctive;the setal counts of the mandible,maxilla,fifth limb,and sixth limb are individual.The locations of the major glands on carapace and the characteristics of the first antenna can be the key of the new species.This work is the second discovery of the genus Polyconchoecia from the world.     

华南两次强台风暴雨过程的水汽与湿位涡对比分析

【目的】探讨不同季节但路径相似的台风暴雨的相关特征,为不同季节的台风暴雨落区预报提供参考依据。【方法】利用常规的探空和地面资料以及NCEP/NCAR1°×1°全球再分析资料,计算2个强台风的水汽通量散度和湿位涡场。对比分析水汽通量辐合、湿位涡正压项(MPV1)和斜压项(MPV2)的水平和垂直分布特征,以及与暴雨落区的对应关系。【结果】秋季的"彩虹"台风高层副热带高压加强,而中低层冷空气和东南气流的汇合使"彩虹"台风的东侧和北侧获得更有利的动力环境条件;而夏季的"威马逊"台风北侧无冷空气影响,台风南侧外围强盛的西南季风气流卷入。台风"威马逊"期间,强的水汽通量辐合中心始终在台风及其残涡中心的南侧和西侧;台风"彩虹"登陆后60 h内一直持续有2支强盛的气流向台风中心输送水汽,而水汽通量的辐合中心与"威马逊"相反,位于台风中心的北侧和东侧,东南气流的卷入以及维持时间长使暴雨增幅。台风"彩虹"登陆后高层高值MPV1扰动下传,低层MPV2> 0并增强,湿斜压性得以增强,有利于垂直涡度增长,使台风低压得以维持和发展;登陆后48～66 h 925 hPa层MPV1为负值,使对流不稳定能量及潜热能的释放,有利于暴雨的维持。而台风"威马逊"登陆后湿斜压性增强不明显。2个台风强降水中心大致位于925 hPa MPV1正负中心过渡带偏向负中心一侧;"威马逊"过程低层MPV1负值中心在正值中心的左侧,对应着西南季风的汇入区;而"彩虹"过程低层MPV1负值中心在正值中心的右侧,对应着冷空气和东南气流的汇合区。这是2个台风暴雨落区差异的成因之一。【结论】本研究得出的湿位涡诊断结果对台风暴雨落区预报具有较好的指示意义。     

Research and application on CSAMT constraint inversion

In this paper, according to the method of electrical constraint inversion, we can improve the accuracy of inversion interpretation using the constraint of electrical logging data in the inversion process, which is conducive to obtain more abundant geoelectric information. On the basis of the conventional OCCAM inversion algorithm, the model roughness in the objective function is improved, and the formula of the electrical constraint inversion iterative algorithm is derived in detail based on the modified objective function. Taking the geothermal exploration in Changbai Mountain area as an example, the measured CSAMT data are used as the resistivity model for inversion, and the electrical property is constrained by the actual data from wells drilled in the section. It can be found that the method of electrical constraint inversion can improve the accuracy of inversion interpretation and guide the development of constrained inversion methods in different ways.     

A Raman investigation on the boron form occurring in salt lake brine during evaporation

Boron resources are abundant in Da Qaidam salt lake of Qaidamu Basin. It has been given great attention for the polyborate species present in brine. In this study, the Raman spectroscopy was applied to investigate the existing-form of boron in brine during evaporation. The prepared solutions of MgO·2B2O3-H2O, MgO·2B2O3-MgCl2 -H2O, and MgO·2B2O3-MgSO4-H2O was also evaporated and recorded to study the influence of boron concentration, pH, and electrolytes on the borate speciation in brine. The mononborates of B(OH)3 and B(OH)4- were found to be the only forms present in the original salt lake brine. Brine evaporation promotes the formation of polyborate anions B3O3(OH)4-, B5O6(OH)4-, and B6O7(OH)62- and also disappearance of the B(OH)4- ion in brine with boron concentration of more than 11 g/L in B2O3. The pentaborate ion of B5O6(OH)4- was sensitive to the solution pH and found to be appeared under the pH value of 8.0. While the hexaborate ion of B6O7(OH)62- was observed more dependent on the electrolyte of magnesium chloride due to its special properties, such as promoting boron accumulation, lowering solution pH, and also the strong af?nity for water molecules, which is beneficial to the polymerization of borate ions in brine. The interaction mechanisms among polyborate anions during evaporation had also been proposed.     

急性高温胁迫对虹鳟二倍体和三倍体幼鱼hsps基因表达的影响

本文以二倍体和三倍体虹鳟(Oncorhynchus mykiss)幼鱼为实验对象,探究急性高温胁迫下,不同倍性虹鳟热休克蛋白家族(hsps)基因表达水平。选取二倍体和三倍体虹鳟幼鱼,分别进行常温处理(14℃)和高温胁迫(22℃),并在高温胁迫48h后,将虹鳟置于常温(14℃)恢复48h。分别在实验开始的0,12,24,48,72和96h进行取样,测定实验虹鳟肝脏和肌肉组织中的hsp70s(hsp70a,hsp70b),hsp90s (hsp90a1a,hsp90a2a,hsp90a1b,hsp90a2a,hsp90b1,hsp90b2)mRNA水平。结果表明:hsp70s和hsp90s的各个亚型均能对热胁迫做出反应,并具有应答幅度的组织特异性和时间特异性。在胁迫6和24h后,上述基因mRNA水平达到最高,随后降低。三倍体幼鱼肝脏和肌肉hsps mRNA表达水平的峰值显著高于二倍体幼鱼。研究结果显示,三倍体虹鳟对高温环境更为敏感,需要消耗更多的能量用于适应高温环境。