A new record of a flathead fish (Teleostei: Platycephalidae) from China based on morphological characters and DNA barcoding

A new record of Platycephalus sp.1 (sensu Nakabo, 2002) was documented based on morphological characters and DNA barcoding. We collected 174 specimens of the genus Platycephalus from Chinese coastal waters of Dongying, Qingdao, Zhoushan, and Beihai. Samples were identified as Platycephalus sp.1 morphologically. The coloration, meristic traits, and morphometric measurements are consistent with previously published records. In brief, it is an orange-brown flathead fish with dark brown spots scattered on head and body, lateral line scales 83 to 99 with one or two spine-bearing anteriormost pored scale, no yellow blotch on the caudal fin. Cytochrome oxidase I subunit (COI) gene fragments were sequenced for phylogenetic analysis. The mean evolutionary distance within the species Platycephalus sp.1 was 0.1%. Net evolutionary distances between Platycephalus sp.1 and other species of Platycephalus ranged from 10.8% to 19.7%, which is much greater than the threshold for species delimitation. The COI sequence analysis strongly supports the validity of Platyceohalus sp.1 at genetic level.     

Omni-directional distribution patterns of montane coniferous forest in the Helan Mountains of China

Slope aspect has significant effect on the development and distribution of montane forest,especially in arid and semiarid regions.This paper,using SPOT5 images and 1:50,000 DEM,digitally extracts and analyzes the spatial information of montane coniferous forest(mainly Qinghai spruce),and thereby explores how the upper and lower limits and the altitudinal range of coniferous forest vary and how the area of coniferous forest is related with annual insolation with all aspects in the Helan Mountains.The results show that: 1) In the eastern flank,the lower limit of coniferous forest is between 1,600 m and 2,000 m a.s.l.,and the upper limit between 2,800 m and 3,000 m a.s.l.;in the western flank,the lower limit of coniferous forest is between 2,000 m and 2,300 m a.s.l.,and the upper limit between 2,800 m and 3,100 m a.s.l.2) The altitudinal ranges of coniferous forest are 806~1,435 m,438~1,140 m for eastern flank and western flank,respectively.3) The area of coniferous forest takes on a normal distribution with aspect,and it has a close relationship with annual insolation.This distribution model developed in this paper quantitatively reveals the significant effect of slope aspect on the distribution of coniferous forest in arid and semi-arid land.     

差分InSAR处理及其应用分析

本文结合实例分析和介绍了影响In SAR处理结果的几个重要环节。如: 数据源及像对选择 ;影响In SAR处理的因素等。目前有4～5种雷达卫星数据可以用于In SAR的处理,ERS-1/2卫星数据效果最好; 差分In SAR处理的工作步骤中,亚像元精度的配准、相干性分析、全相位恢复等是其中的关键技术。In SAR的精度和噪声水平是In SAR能否实用的重要条件,噪声影响地物的相干性,噪声较大部位相干性消失,在整幅图像中噪声区域不大于30% ,将不影响整体的In SAR测量结果。     

中南4省(区)产铀层基本特征及其与铀成矿的关系

豫鄂湘桂4省(区)共有49个产(富)铀层组.主要有上震工统一下寒武纯、中泥盒统,下二叠统及上由叠统.其时空分布与地壳演化有关.受特定的构造捷回和大地构造单元控制.岩性为一定地质时期形成的海相碳硅泥岩建造和河流一滨湖三角洲相砂岩建造.富铀层往往是极重要的铀源层及良好的储铀层.产(富)铀层的岩性序列组合,岩石的化学和物理性质及构造一岩浆作用的叠加,严格控制着铀矿化的形成和富集.     

一株H_5N_1亚型禽流感病毒株NA基因的克隆与序列分析

用RT-PCR方法从1个H5N1亚型禽流感病毒分离株A/Chicken/Guangdong/DH/1997扩增NA基因cDNA片段,将其克隆至pMD18-T载体,获得重组质粒pMD-NA,并对其核苷酸序列进行测定和分析。结果表明,该毒株的NA基因长度为1350bp,编码449个氨基酸,与其它H5N1亚型AIV分离株的核苷酸序列同源性为97.0%～99.4%,氨基酸序列同源性为97.7%～99.1%,提示禽流感病毒NA基因保守性较高。NA基因氨基酸序列的聚类分析表明该毒株与来自香港的A/Pheasant/HK/FY155/01和A/Ch/HK/FY150/01两个分离株处于同一进化枝,亲缘关系较近。     

印尼西加里曼丹寒梅山金矿床矿物蚀变特征及找矿方向

寒梅山(音译)金矿位于印度尼西亚西加里曼丹省西北部,是全球特提斯成矿域、环太平洋成矿域与印度?澳大利亚成矿域的交汇地带,具有较大的金矿找矿潜力.文章通过对矿区开展矿物蚀变填图,识别出青磐岩化带、泥化带、中度泥化带、绢英岩化带、钾化带、硅化带等6个矿物蚀变带,具有斑岩?浅成低温热液型矿床的矿物蚀变分带特征.结合区域地质特...     

Molecular Characterization and Expression Analysis of SKIV Infection of Interferon-Induced Protein with Tetratricopeptide Repeats 1(IFIT1) in Epinephelus lanceolatus

Interferon-induced protein with tetratricopeptide repeats 1(IFIT1), also known as interferon-induced protein 56(IFI56) or Interferon-stimulated protein 56(ISG56), was originally identified as a protein induced upon treatment with interferon and inhibited by viral replication and translational initiation. In this study, Epinephelus lanceolatus IFIT1(ELIFIT1) gene was cloned for the first time. The complete cDNA of El IFIT1 gene includes 2921 nucleotides, and encodes a 437-amino acid(AA) protein. The putative ELIFIT1 protein has 9 TRP domains and is highly similar with IFIT1 proteins in other teleosts. In healthy fish, ELIFIT1 gene was highly expressed in the blood, which indicate its specific function in the peripheral immune system. Its expression was also observed in various immunity-related tissues including spleen, intestine, and kidney, Inducted with spotted knifejaw iridovirus(SKIV), ELIFIT1 gene expression was upregulated in the spleen, kidney, and liver 24 h after induction and reached its peak at 72 h, indicating that ELIFIT1 may play an important role in antivirus. These findings contribute to the understanding of the antiviral regulation of ELIFIT1 gene in teleost.     

渤海湾西岸全新世海面变化

在讨论海面标志点基本定义的基础上,从渤海湾西岸534个原始数据中获得了136个指示高水位、潮间带或低水位的海面变化标志点,并重建了它们的相对海平面时空分布,进而获得了渤海湾西岸全新世相对海平面变化带.约10cal.ka B.P. 时,相对海平面变化带达到-25m,以约6m/1000a(即约6mm/a)的平均速率上升,在6cal.ka B.P. 前后达到现代海面的高度; 6cal.ka B.P. 至今,变化带的高度介于+1m~-2m之间,未发现中全新世相对高海面.再搬运海相贝类和陆相泥炭类样品的 14C 年龄,分别存在约600年和660年的驻留时间.经过驻留时间校正的新海面变化年代学序列,将渤海湾相对海平面达到现代高度的时间点确定为约6cal.ka B.P.,从而与全球海面变化的对比更为准确.渤海湾盆地的长期稳定下沉和沉积自重压实的共同作用,可能抵消了冰川均衡调整(GIA)引起的中全新世数米高的相对高海面.     

赞比亚铜矿床类型及地质特征

赞比亚是世界著名的铜产地之一,其铜矿类型可分为层控型、沉积型、变质改造型及热液型,以沉积型为主。据现有大中型矿床铜资源量统计,沉积型矿产铜资源量占赞比亚铜总资源量的79%。各类型铜矿床总体受卢弗里安(Luflian)弧控制,层控型铜矿主要分布在穹窿核部边缘,含矿岩系为莫瓦(Muva)超群的黑云母片岩、二云石英片岩;沉积型铜矿主要分布在背斜或穹窿外缘周围,含矿岩系主要是加丹加(Katanga)超群下部的砂页岩层。     

应用荧光抗体技术检测牙鲆体内的河流弧菌

用灭活的河流弧菌(Vibrio fluvialis)抗原,注射免疫实验兔,制得凝集价为1∶5 120的抗血清。用试管凝集法检测了抗血清的特异性,再用免疫吸附法去除交叉反应,从而得到高效价、高特异性的抗河流弧菌血清。用所制备的抗血清在实验室中建立起河流弧菌荧光抗体检测技术(FAT),在荧光显微镜下可清楚地看到被标记的病原菌,整个检测过程只需3h。用河流弧菌感染牙鲆(Paralichthys olivaceus),24 h后,用FAT测定牙鲆的血液、肾脏和肝脏中的河流弧菌,在血液中河流弧菌的检出率最高,其次是肾脏,肝脏的检出率最低。以上结果表明:荧光抗体技术可以快速、灵敏、准确地检测出牙鲆体内的河流弧菌。