A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China

Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A cuhure-dependent survey of microbial population in the Xiamen hot springs was pcrformed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilie thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55-90℃ including Rhodothermus marinus (Strain 1) , Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3) , Geobacillus stearothermophilus(Strain 4) , Geobacillus thermoleovorans (Strain 5) , and Pseudomonas pseudoal-caligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50℃ and a pH range of 1. 8-3.5 such as Alicyclobacillus acidoterrestris (Strain 8) , Sul-fobacillus acidophilus (Strain 9), and Sulfobacillus thermosulfidooxidans (Strain 10), were isolated on selective solid medium containing sulfur and Fe2+. Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophilcs, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene se-quence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodother-mus marinus and Thermus thermophilus dominated the cultivable microbial community.     

产芝水库的浮游植物与水体水质评价

2006年在产芝水库共鉴定浮游植物8门56属82种,其中种类最多的是绿藻和硅藻,分别占总种数的32.93%、31.71%。浮游植物种类四季变化明显,入口、中心和出口浮游植物群落结构也有一定差异,库区优势种为尖尾蓝隐藻(Chroomonas acuta)、小环藻(Cyclotell asp)、尖针杆藻(Synedra acus)等。水库浮游植物的四季平均细胞密度为9.048×105/L,出口细胞平均密度最高,达18.007×105/L。叶绿素a的年均值为7.33 mg/m3,综合营养状态指数为44.21。从浮游植物的群落结构、叶绿素a含量和综合营养状态指数来看,水库处于中营养水平。     

褐藻酸降解菌A7的发酵及产酶条件研究

以海藻废弃物堆肥中分离到的薄壁杆菌属（Gracillibacillus）的褐藻酸降解菌A7为研究对象,对该菌的生长及产酶条件进行了研究。结果表明,该菌的最适产酶条件为：温度30℃,海藻酸钠的质量分数0．5％,pH9．5,NaCl浓度0．5mol／L,以蛋白胨为主要氮源。在最佳条件下培养96h达到最高酶活力12．79U／mL。     

一株虹鳟源枯草芽孢杆菌的分离鉴定及生物学特性研究

为筛选对虹鳟(Oncorhynchus mykiss)有潜在益生效果的菌株,本研究从健康虹鳟幼鱼肠道中分离到1株潜在益生菌株RT-BS07,采用形态学观察和分子特征分析对该菌株进行了鉴定,并评价了该菌株的抑菌性、耐受性、黏附性和安全性。形态学观察显示,菌株RT-BS07为革兰氏阳性杆菌,有圆形或椭圆形芽孢。16S rRNA比对结果显示,该菌株与枯草芽孢杆菌(Bacillus subtilis)NCBI3610株的同源性为99.86%。结合16S rRNA基因序列分析和形态学观察结果,确定菌株RT-BS07为枯草芽孢杆菌。菌株RT-BS07具备产蛋白酶和纤维素酶能力,其酶活力分别为(0.039±0.002)和(0.393±0.002)U/mg;该菌株可有效抑制温和气单胞菌(Aeromonas sobria)、杀鲑气单胞菌(A.salmonicida)和鲁氏耶尔森氏菌(Yersinia ruckeri)等致病菌。菌株RT-BS07在pH为2~10 LB液体培养基中均可存活;在盐度为2%~8%条件下存活率为5.70%~93.28%,其存活率随盐度升高而降低。体外黏附试验结果表明,菌株RT-BS07可大量黏附在虹鳟前肠黏膜,不破坏肠道组织完整性,对左氟沙星、环丙沙星、氧氟沙星和链霉素等18种抗菌药物敏感。本研究分离筛选得到的枯草芽孢杆菌RT-BS07株具有产消化酶性能,其抗逆性能强,对虹鳟无毒害作用,可作为虹鳟健康养殖及益生菌制剂研发源益生菌候选株。     

海南产芋螺的形态特征

从海南各个市县沿海采集到20多种芋螺（Conus）,并制成标本,对这些芋螺标本进行了详细的外部形态特征测量与描述,包括芋螺壳形状、大小、体质量、壳顶特点、表面光滑度、螺纹、色彩及花纹等。鉴定出20种海南产的芋螺种类,为芋螺毒素基因的分离和芋螺毒素药物的开发提供基础。     

黄土坡面不同侵蚀带侵蚀产沙关系及其机理

黄土梁峁坡面土壤侵蚀具有明显的垂直分带性,不同侵蚀带具有各自的侵蚀产沙特点,且彼此相互影响,坡面侵蚀产沙分配受降雨强度和降雨能量的制约,随着雨强和降雨能量的增加,坡面侵蚀产沙量的最大部位由细沟侵蚀带向浅沟侵蚀带过渡,坡面侵蚀产沙强度变化的本抽为侵蚀方式的演变,坡面复合侵蚀带的径流模数明显小于对应的单一侵蚀带径流模数,而其侵蚀模数则明显大于对应的单一侵蚀带侵蚀模数,溅蚀片蚀带的来水来沙可吏细沟侵蚀带     

长江黄河源区覆被变化下降水的产流产沙效应研究

在长江和黄河源区的左冒西孔曲和纳通河、垮热洼尔玛河流域的不同植被覆盖下建立了天然径流观测场,利用观测天然降水和人工模拟降水,初步研究了江河源区不同植被覆盖下降水的产流产沙效应。结果表明,长江黄河源区的3个小流域内,在典型高寒草甸草地30°坡面上,退化较为严重的30%覆盖度以下的场地内,地表径流产出量明显大于覆盖度较好的95%、92%和68%场地,同时产沙量显著高于这3个场地,其平均单次降水形成的泥沙量是这三种盖度的2~4倍,由此造成地表侵蚀量平均为这3种盖度的3~10倍。通过对几次典型的降水形态的分析,在长江黄河源区高寒草甸草地的坡面上,不但降水量影响着产流产沙量,降水形态也影响着产流产沙量,降雨仍是引起水土流失的主要降水形态,在降水量相同的条件下,降雪可比降雨和雨加雪增加产流量2.1~3.5倍,可比降雨减少泥沙侵蚀45.4%~80.3%。人工模拟结果表明:对于覆盖度为5%和30%的强度退化草地,次降水量在3.5 mm时,就形成了较为明显的径流和产沙效应,当次降雨量达到7 mm,降雨持续时间15 min,5 m2场地内就会形成1 400 mL以上的径流量;在地表土壤含水量(FDR测0~5 cm平均含水量为36.7%)较高的情况下,次降雨量达4mm,降雨强度超过0.4 mm/min,在5 m2场地内历时5 min就能形成1 060 mL的地表径流,每100 mL径流中含泥沙高达1.6 g。这一试验结果在长江黄河源区3个不同的河源小流域是一致的。     

亚热带6种老龄天然林及杉木人工林的枯落物持水性能

通过对福建建瓯万木林自然保护区6种老龄天然林:木荷(Schima superba,SCS)、罗桴栲(Castanopsis fabri,CAF)、观光木(Tsoongiodendron odorum,TSO)、浙江桂(Cinnamo-mum chekiangense,CIC)、细柄阿丁枫(Altingia gracilipes,ALG)、米槠(Castanopsis carlesii,CAC)及杉木人工林(Cunninghamia lanceolata,CUL)枯落物现存量和持水特性的研究表明,各林分枯落物现存量分别为木荷7.22 t.hm-2、细柄阿丁枫6.79 t.hm-2、浙江桂6.70 t.hm-2、观光木6.66 t.hm-2、罗桴栲6.57 t.hm-2、米槠5.96 t.hm-2和杉木人工林4.82 t.hm-2.最大持水率平均值是人工林(207.98%)天然林(179.38%).各林分最大持水量分别为木荷13.33t.hm-2、细柄阿丁枫13.32 t.hm-2、观光木13.18 t.hm-2、罗桴栲10.84 t.hm-2、浙江桂10.52t.hm-2、米槠10.30 t.hm-2和杉木人工林9.95 t.hm-2.枯落物平均现存量和平均最大持水量均为天然林(6.65 t.hm-2,11.95 t.hm-2)大于杉木人工林(4.82 t.hm-2,9.95t.hm-2).影响林地枯落物持水量的主要因素是枯落物现存量.各林分主要树种半分解层的枯落物现存量及最大持水量均大于未分解层.     

沙漠沟谷暴雨洪水侵蚀产沙特征

在半干旱区的季节性沙漠沟谷,暴雨引发的洪水过程侵蚀产沙强度大,水土流失严重,对区域及下游河道生态造成严重威胁。以毛布拉孔兑的支沟苏达尔沟为研究对象,以苏达尔沟2011—2015年6次暴雨洪水事件的观测数据为基础,分析洪水流量、泥沙浓度及地表沉积物粒度特征,给出暴雨洪水侵蚀产沙输沙特征。结果表明:观测期间暴雨洪水侵蚀产沙量平均每次约37.69×10~4t,产沙模数为0.57×10~4t·km^-2;其中最大的洪水事件130721号暴雨洪水过程侵蚀产沙量高达90.47×10~4t,产沙模数达1.36×10~4t·km^-2。流域总侵蚀产沙以0.25～0.063 mm泥沙为主,约占总侵蚀量74%。洪水总侵蚀产沙量随暴雨产流强度增强而增加,同时下游沙漠沙地段产沙贡献比重也随之增加,风沙贡献也相应增大。坡面侵蚀约占暴雨洪水总侵蚀的4.37%,且主要集中在上游砒砂岩坡面。     

Simulated multi-scale watershed runoff and sediment production based on GeoWEPP model

The runoff and sediment yield data from the Qiaozidonggou, Qiaozixigou, and Lu＇ergou watersheds, in the Loess Plateau of China are used to calibrate and validate the runoff and sediment yield simulated by GeoWEPP model of the WEPP Model at watershed scale. The indices of relative error, R, correlation coefficient, Re, and Nash-Suttcliffe efficiency coefficient Ens are used to evaluate the model fit. The eco-hydrological responses in the Luoyugou and Lu＇ergou watersheds are also forecast based on the WEPP Model. Meanwhile, the relation between vegetation pattern changes and sediment yield in the watershed is discussed, and the responses of runoff and sediment yield in the watersheds concerning forest growth stages are studied. The results show that the relative errors of simulated values of runoff and sediment yield are below 30%, the correlation coefficients axe above 0.90, and the Nash-Suttcliffe efficiency coefficients axe above 0.80. The simulation results present satisfactory performance, thus, the model could be used to simulate the runoff and sediment yield in these small watersheds. It is also observed that soil erosion tended to become severe as precipitation increased in the watershed, while soil erosion has a decreasing trend as forest cover increases and vegetation pattern is optimized. When the watershed is fully covered by forest, erosion and sediment yield are minimized. When the forest cover is about 30% and evenly distributed in the watershed, the erosion intensity is lower than if the forest cover is collectively distributed in the watershed. Erosion varies with different forest growth stages in the watershed; it is more serious at the young and near planting stage and is the smallest at the mature forest stage.