Molecular Characterization and Expression Analysis of SKIV Infection of Interferon-Induced Protein with Tetratricopeptide Repeats 1(IFIT1) in Epinephelus lanceolatus

Interferon-induced protein with tetratricopeptide repeats 1(IFIT1), also known as interferon-induced protein 56(IFI56) or Interferon-stimulated protein 56(ISG56), was originally identified as a protein induced upon treatment with interferon and inhibited by viral replication and translational initiation. In this study, Epinephelus lanceolatus IFIT1(ELIFIT1) gene was cloned for the first time. The complete cDNA of El IFIT1 gene includes 2921 nucleotides, and encodes a 437-amino acid(AA) protein. The putative ELIFIT1 protein has 9 TRP domains and is highly similar with IFIT1 proteins in other teleosts. In healthy fish, ELIFIT1 gene was highly expressed in the blood, which indicate its specific function in the peripheral immune system. Its expression was also observed in various immunity-related tissues including spleen, intestine, and kidney, Inducted with spotted knifejaw iridovirus(SKIV), ELIFIT1 gene expression was upregulated in the spleen, kidney, and liver 24 h after induction and reached its peak at 72 h, indicating that ELIFIT1 may play an important role in antivirus. These findings contribute to the understanding of the antiviral regulation of ELIFIT1 gene in teleost.     

核电站温排水对大亚湾鲷科鱼卵,仔鱼分布的影响

在广东大亚湾核电站温排水排放前、后，自１９９３年１月２０日至１９９５年２月２８日对该站附近水域１４个站位进行６个航次的采样调查，收集鲷科鱼卵、仔鱼样本和同一站位的水温资料。结果表明，各站位的鲷科鱼卵、仔鱼的分布在温排水排放前、后与水温变化密切相关。水域升温２￣８℃对鲷科鱼类的产卵、孵化和仔鱼生长发育有明显的影响。鉴于目前升温水域范围只限在２ｎ ｍｉｌｅ内，可以认为整个大亚湾鲷科鱼类的资源和补充尚水     

黄土振动促渗效应研究

在大规模高铁和重载铁路建设与运营过程中,出现了一类新型黄土灾害,即机车振动、降雨和干湿循环联合作用下诱发的铁路路基黄土湿陷振陷与斜坡体崩解滑移.这对于铁路毫米级变形控制而言后果不堪设想,严重地威胁着铁路运营与人民生命安全.为此,作者提出了黄土受振动、水和干湿循环等影响下的"五促效应",即促裂、促渗、促崩(解)、促滑和促...     

五台山区构造对铁矿的控制规律浅析

五台山区富含铁矿资源。区域构造以多期次运动叠加的褶皱为主,后期构造对前期构造进行了改造。构造外貌总体呈“之”字型展布。区域构造以及其次级构造对矿体（床）有明显的控制作用,表现为区域构造控制矿体（床）的分布,二级构造控制矿体（床）的形态,三级以下构造对矿体（床）起局部控制或改造作用。区域铁矿床亦受“之”字型的控制。     

2013年南斯科舍海岭MW7.8地震的多点震源机制反演

2013年11月17日,在南极南奥克尼群岛北、南极板块与斯科舍板块之间发生了一次M_W7.8级地震(2013年南斯科舍海岭M_W7.8地震),我们利用全球分布的长周期和宽频带地震记录反演确定了这次地震随时间和空间变化的震源机制,验证了提出的一种多点震源机制反演的新方法.首先利用长周期记录的W震相反演了这次地震的矩心矩张量解并利用体波提取了视震源时间函数,同时利用台阵反投影技术从宽频带记录中获得了这次地震的高频源的时空分布,然后基于矩心矩张量解、视震源时间函数以及高频源的时空分布,实现了采用新方法对2013年南斯科舍海岭M_W7.8地震的多点震源机制反演.矩心矩张量解表明,地震矩心在44.50°W/60.18°S,矩心深度19 km,半持续时间49 s,释放标量地震矩4.71×10²⁰ N·m,发震断层走向104°,倾角54°,滑动角8°.视震源时间函数清楚地揭示了地震矩随时间变化的方位依赖性,总体上可以将时间过程分为前60 s和后50 s两个阶段,但前60 s可细分为两次子事件.根据台阵反投影结果,这次地震为沿海沟从西到东的单侧破裂,破裂长度达311 km,可以分为5次子事件,能量释放的峰值点依次为13 s、30 s、51 s、64 s和84 s,平均破裂速度分别为0.6 km·s^-1、2.6 km·s^-1、2.3 km·s^-1、2.8 km·s^-1和3 km·s^-1.多点震源机制反演显示,5次子事件的矩震级分别为M_W7.57,M_W7.48,M_W6.80,M_W7.53和M_W7.08,半持续时间依次为21 s,17 s,6 s,16 s和8 s,走向分别为95°,105°,81°,98°和98°,倾角依次为57°,49°,86°,46°和64°,滑动角-9°,1°,-17°,13°和-4°.这些在震源机制、能量释放以及持续时间方面的变化都是当地构造和应力环境复杂性的反映.

     

基于面向对象CART决策树的土地利用分类研究

面向对象的CART决策树分类方法可解决目前流行的监督分类、非监督分类以及模糊分类方法中“同物异谱、异物同谱”引发的漏分、错分问题。该方法融入了形状和纹理特征进行分类,同时运用二级分类体系解决了相似地物因光谱、纹理不同而导致的地物错分问题,分类效果较好。利用楚雄市鹿城镇2013年GF-1号遥感影像进行土地利用分类。结果表明：(1)基于光谱、形状和纹理信息选取的19个特征变量开展面向对象的CART决策树分类,总体精度可达90.22%,其中林地分类的效果最好;(2)二级分类体系解决了耕地、裸地因光谱、纹理特征多样而产生的地物错分问题,总体精度提高了7.06%,Kappa系数提高了8.17%。     

张仲景火疗法运用探讨

通过对张仲景所述灸法、温针、烧针、熏法、熨法五种火疗法进行分析,探讨火疗法的运用。火疗法具有温热性质,宜用于阴证与寒证。其禁忌有三,一为太阳表证慎用火疗法,以防邪气郁闭不得出;二为阳明热证不可用火疗法,以防“两阳相劫”加重热证或伤津出现变证;三为阴虚不足不可用火疗法,以防火热之性灼伤津液,迫津外泄,阴损及阳。若误用火疗法则会出现烦躁、谵语等变证。了解其运用和禁忌,可指导临床合理地、正确地使用火疗法。     

黑鲷音响驯化初步试验

利用音响对鱼进行驯化,在国内是一个新课题,但在国外早已开展了这方面的工作,其研究鱼类听觉的结果表明,大多数鱼对低频声感觉较敏感。鲷科的可听音域为36—1250Hz其中真鲷在低频段的最高敏感频率为200Hz而线笛鲷在低频段的最高敏感频率为400—600Hz。本试验采用低频段的100—600Hz的纯音对黑鲷做了音响驯化试验。试图从中找出黑鲷对音响反应的频率范围、音响驯化的音敏频率和记忆时间等有关要素。     

江汉五桥深水基础施工技术

以江汉五桥主桥施工为例，介绍了桥梁深水基础施工方案的选定及施工要点，重点对砂层长钻孔桩施工、钢护筒渡汛及钢套箱围堰施工进行了分析。     

Extraction and PTP1B inhibitory activity of bromophenols from the marine red alga Symphyocladia latiuscula

Previously, we had characterized several structurally interesting brominated phenols from the marine red alga Symphyocladia latiuscula collected from various sites. However, Phytochemical investigations on this species collected from the Weihai coastline of Shandong Province remains blank. Therefore, we characterized the chemical constituents of individuals of this species collected from the region. Eight bromophenols were isolated and identified. Using detailed spectroscopic techniques and comparisons with published data, these compounds were identified as 2,3-dibromo-4,5-dihydroxybenzyl methyl ether (1), 3,5-dibromo-4-hydroxybenzoic acid (2), 2,3,6-tribromo-4,5-dihydroxymethylbenzene (3), 2,3,6-tribromo-4,5-dihydroxybenzaldehyde (4), 2,3,6-tribromo-4,5-dihydroxybenzyl methyl ether (5), bis(2,3,6-tribromo-4,5-dihydroxyphenyl)methane (6), 1,2-bis(2,3,6-tribromo-4,5-dihydroxyphenyl)-ethane (7), and 1-(2,3,6-tribromo-4,5-dihydroxybenzyl)-pyrrolidin-2-one (8). Among these compounds, 1 and 2 were isolated for the first time from S. latiuscula. Each compound was evaluated on the ability to inhibit protein tyrosine phosphatase 1B (PTP1B), which is a potential therapeutic target in the treatment of type 2 diabetes. Bromophenols 5, 6, and 7 showed strong activities with IC50 values of 3.9, 4.3, and 3.5 μmol/L, respectively. This study provides further evidence that bromophenols are predominant among the chemical constituents of Symphyocladia, and that some of these compounds may be candidates for the development of anti-diabetes drugs.