中国南海一株固氮类芽孢杆菌的筛选和分离鉴定

为了解中国南海海洋自生固氮菌的种类,作者对采集的南海海底淤泥样品进行了固氮微生物的分离、筛选及鉴定。经过土样沸水加热处理,无氮培养基平板初筛后,对分离获得的细菌固氮酶结构基因nif H进行扩增,并对其固氮酶活性进行检测,最终获得一株能够产芽孢的固氮细菌。对该菌株进行生理生化性状测定、16S r DNA序列分析(Gen Bank登录号KJ627376),并基于nif H、16S r DNA系统进化树分析,确定该菌为一株固氮类芽孢菌(Paenibacillus sp.)NH-1。本研究表明固氮类芽孢杆菌在海洋中确有分布,海洋自生固氮菌的多样性远远超出人们之前的认识。     

Paleomagnetic and palynologic analyses of Albian to Santonian strata at Bayn Shireh, Burkhant, and Khuren Dukh, eastern Gobi Desert, Mongolia

Cretaceous terrestrial sediments deposited in a series of intracratonic basins across the Gobi Desert region of southern Mongolia and northern China contain a unique and diverse vertebrate fauna. In 1996 an expedition jointly sponsored by the Mongolian Paleontological Center and the Hayashibara Museum of Natural Sciences revisited a number of famous vertebrate fossil localities in the eastern Gobi region of Mongolia and, as part of a broad geological and paleontological study, collected a series of paleomagnetic samples from measured sections at Bayn Shireh, Burkhant and Khuren Dukh, as well as from an unmeasured locality adjacent to Khuren Dukh. Expedition members also collected palynologic samples from Khuren Dukh and the adjacent locality. Paleomagnetic analysis shows that all the sites from which samples were collected display detrital remnant magnetization that is consistently normal in polarity. The measured Cretaceous magnetic directions are oriented to the east or northeast of the present day expected direction (declination 356.2°, inclination 65.2°), and they are wholly concordant with that expected for a mid-latitude Northern Hemisphere sampling locality, and with the directions for this period reported by other workers. These results, when considered in tandem with the known biostratigraphy, strongly suggest that the sedimentary deposits at all four localities in the eastern Gobi correlate to the normal polarity chron 34 (the Cretaceous Long Normal), which ranges in age from approximately 121 to 83.5 million years. Previous vertebrate, invertebrate and palynological data from Khuren Dukh suggest that the lower and middle parts of the stratigraphic interval exposed there (which have been assigned to the Shinekhudag Formation) are ‘Khukhtekian’ in age and correspond to the Aptian–Albian interval that can be broadly correlated to the older, Early Cretaceous part of the Cretaceous Long Normal, C34n. New palynologic data presented here indicate that these strata are no older than middle to late Albian. The rocks at Bayn Shireh (the Bayn Shireh Formation) have been assigned a ‘Baynshirenian’ biostratigraphic age that may range from Cenomanian to early Campanian. The magnetostratigraphy results presented here indicate that the strata at both the Bayn Shireh and Burkhant localities do not cross the Santonian/Campanian Stage boundary, however, as this is believed to lie at, or very near, the C34n/C33r reversal boundary. Thus, the Bayn Shireh Formation was most likely deposited near the end of the Cretaceous Long Normal Interval, no later than the latest Santonian.     

IAEA-S-1 参考物质及V-CDTS 同位素标准的32S/34S绝对比值

采用高纯S同位素物质和SF6的分析方法对IAEA-S-1参考物质及V-CDT S同位素标准的32S/34S绝对比值进行了实验标定，V-CDT的32S/34S比值为22.6496±0.060，IAEA-S-1的32S/34S比值为22.6564±0.0060.     

徐州市水土流失时空变化研究

本文以3S技术为支撑,在修正水土流失方程（RUSLE）的基础上,针对徐州黄泛平原-丘陵地带的特殊地形地貌、对2000年以来徐州市水土流失时空变化特征进行了分析。结果表明：2000-2014年徐州市土壤侵蚀等级以微度为主,微度侵蚀面积占总侵蚀面积多年平均达到76.34%;中度侵蚀次之,所占比例在10%~17%间;剧烈侵蚀所占比例最少。轻度及以上土壤侵蚀等级主要发生在中部微山湖下游、京杭大运河一带的丘陵岗地地区、主城区与故黄河北岸的黄泛平原地区,以及丰县、新沂、邳州的局部区域;土壤侵蚀存在一定程度年际变化特征,睢宁、丰县、邳州的部分地区侵蚀面积年际变化较为明显,土壤侵蚀总面积整体上呈现下降趋势。     

Quality Assessment of Frozen Solenocera crassicornis Treated with Sodium Metabisulphite by Soaking or Spraying

The present work was carried out to evaluate the safety of shrimp(Solenocera crassicornis)treated with different concentrations of sodium metabisulfite(SMB)by soaking or spraying during frozen storage.Shrimps soaked in higher concentrations of SMB showed higher sensory scores,lower total color differences,and better anti-melanosis effects than shrimps in the control and other treatment groups throughout frozen storage(−18℃).Lower total volatile basic nitrogen and thiobarbituric acid reactive substances and higher salt soluble protein contents were detected in shrimp soaked with high doses of SMB compared with other samples.In addition,lower counts of total aerobic plates and psychrotrophic bacteria were observed in shrimp treated by soaking with higher doses of SMB than those in control shrimp and shrimp treated with other methods during frozen storage(−18℃).However,the SO2 content of 5%SMB-soaked samples exceeded the maximum allowable limit of 100mgkg−1.Overall,the use of 1.5%SMB soaking to treat shrimp results in good antioxidant and antimicrobial effects and,thus,may be suggested to preserve S.crassicornis under frozen conditions.The results of this study present important guidance on the use of SMB to maintain the quality of marine-trawling shrimp from manufacturing to consumption.     

基于C/S的矿产资源开发储量管理信息系统的研发

为实现矿产资源储量管理网络实时查询功能,在目前国土资源部金土工程的储量管理软件的基础上,开发出了"基于C/S的矿产资源开发储量管理信息系统".该系统采用了结构化的程序设计方法,操作系统平台采用了MS Windows系统,程序开发平台采用了Borland公司Delphi,数据库的查询语言采用标准SQL查询语言,主要实现了矿产资源开发和储量数据的综合查询和汇总功能.     

The effects of temperature and substrate on ontogenetic behavior of bastard halibut, Paralichthys olivaceus (T. et S.)

1 INTRODUCTION Scientific management of aquacultural andnatural populations studies on the early life historyof flatfishes in the aspects of their metamorphosisand benthic settlement accompanying pronouncedtransitions in morphology, behavior and habitatecology have received much attention (Youson,1988). Accumulated data showed that temperatureand substrate played a main role in the recruitmentsuccess of flatfish during their early development(Rijnsdorp et al., 1995, Gibson and Robb, 2000…     

Screening and molecular classification of low-temperature protease from Antarctic microorganism and its characterization

107 strains producing protease were screened from 260 strains of Antarctic psychrophilic bacteria, among which proteolytic activity of five strains was more than 45 U ml^-1. The 16S rRNA gcne sequences homology and phylogcnetic analysis of five Antarctic psychrophillc bacteria showed that NJ276, NJS-9, NJ16-70,NJ345 belonged tO the described genus Pseudoalteromonas and NJ341 belonged to the genus Colwellia. The growth and the protease characteristic of four Antarctic psychrophilic bacteria had been studied, and the result showed that the 6ptimal temperature for growth and protease-produeing of four strains was about 10℃. Their growth and protease-produeing were still high during incubatlng 2-5 days. The maximum proteolytic activity occurred at pH 9 for four Antarctic psychrophilic bacteria. The optimal temperature of protease action of both strains NJ276 and NJ5-9 was about 50℃, however, the optimal temperature of protease aetlon of both strains NJ341 and NJ345 was about 40 ℃, and their proteolytic activity under 0℃ exhibited nearly 30% of the maximum activity, but their thermal stabilities were weaker. These results indicated that proteases from NJ341 and NJ345 were low-temperature proteases.     

信息安全实验管理系统的实现及其系统中安全问题的解决

B／S模式的应用系统都要面对安全的问题，身份认证和访问控制是实现计算机安全的主要手段。介绍了基于Web的信息安全实验管理系统，分析了系统的体系架构和功能，针对B／S模式的安全威胁，采用了两个方案实现身份认证和访问控制。     

线性Radon变换噪音压制法及其在古龙断陷中的应用

针对松辽盆地北部古龙断陷地震资料信噪比低、线性干扰强特点,提出应用线性Radon变换进行叠前线性噪音压制的预处理方法,Radon变换可在炮集和CMP道集上进行运算,算法简单,易于编程实现,其积分路径的特点适合线性噪音压制.模拟数据和实际地震资料应用结果表明,线性Radon变换法能够实现保幅的线性噪音压制,是叠前提高地震资料信噪比的实用方法,在地震资料预处理中具有应用前景.