Dissolved inorganic carbon (DIC) and its carbon isotopic composition in sediment pore waters from the Shenhu area,northern South China Sea

The Shenhu area is one of the most favorable places for the occurrence of gas hydrates in the northern continental slope of the South China Sea. Pore water samples were collected in two piston cores (SH-A and SH-B) from this area, and the concentrations of sulfate and dissolved inorganic carbon (DIC) and its carbon isotopic composition were measured. The data revealed large DIC variations and very negative δ ¹³C-DIC values. Two reaction zones, 0–3 mbsf and below 3 mbsf, are identified in the sediment system. At site SH-A, the upper zone (0–3 mbsf) shows relatively constant sulfate and DIC concentrations and δ ¹³C-DIC values, possibly due to bioturbation and fluid advection. The lower zone (below 3 mbsf) displays good linear gradients for sulfate and DIC concentrations, and δ ¹³C-DIC values. At site SH-B, both zones show linear gradients, but the decreasing gradients for δ ¹³C-DIC and SO₄ ²⁻ in the lower zone below 3 mbsf are greater than those from the upper zone, 0–3 mbsf. The calculated sulfate-methane interface (SMI) depths of the two cores are 10.0 m and 11.1 m, respectively. The depth profiles of both DIC and δ ¹³C-DIC showed similar characteristics as those in other gas hydrate locations in the world oceans, such as the Blake Ridge. Overall, our results indicate an anaerobic methane oxidation (AMO) process in the sediments with large methane flux from depth in the studied area, which might be linked to the formation of gas hydrates in this area.     

Factors controlling dissolved silica in coral reef surface water of Urasoko Bay, Ishigaki Island, Japan

From August 2006 to August 2007, the concentrations of dissolved silica (Si(OH)₄) were monitored in the surface water of Urasoko Bay and the mouth of the stream that runs into the bay. Urasoko Bay is located on the northern coast of Ishigaki Island, Okinawa, Japan, which is in a subtropical area of the North Pacific Ocean and is surrounded by a relatively poorly developed fringing reef. Added to these samples were freshwater from the upstream area and brackish water that exudes at the beach site, which were collected from April to June 2007. Rainwater samples were also collected during the study period. The concentration of Si(OH)₄ generally decreased from upstream to the bay site, and, on clear days, Si(OH)₄ data from all study sites (the bay, beach, stream mouth, and upstream) plotted against salinity fell on a single straight line. When the influence of rainwater was, the results were scattered below the straight line, which suggests dilution by rainwater with a much lower Si(OH)₄ concentration. These findings show that offshore seawater, rainwater, and upstream freshwater regulate the concentration of Si(OH)₄ in the surface water of Urasoko Bay.     

军曹鱼染色体组型分析

为了解军曹鱼(Rachycentron canadum)的细胞遗传学特征,采用植物血球凝集素(PHA)、秋水仙碱胸腔注射,取头肾细胞经空气干燥法制片,分析了军曹鱼染色体核型。结果表明,军曹鱼核型为:2n=48=48t,染色体总臂数(NF)为48。     

6种鱼类肌肉组织肌苷酸的检测分析

采用高效液相色谱技术,在国内首次测定了条纹斑竹鲨(Chilocsyllium plagiosum)、大黄鱼(Pseudosci-aena crocea)、鳜(Siniperca chuatsi)、青石斑鱼(Epinephelus awoara)、牙鲆(Paralichthys olivaceus)和乌塘鳢(Bostrichthys sinensis)等6种鱼类新鲜和冻存肌肉组织中肌苷酸含量。结果表明:(1)不同鱼类肌肉组织中肌苷酸质量比不同,牙鲆新鲜肌肉组织中肌苷酸质量比最高,为4.214 mg/g,其余5种鱼类为2.365~3.634 mg/g。(2)10 d冻存试验中,经-70℃超低温冻存,6种鱼类冻存肌肉组织中肌苷酸质量比均高于新鲜肌肉组织,大黄鱼肌肉组织冻存1 d肌苷酸质量比达最大值,鳜肌肉组织冻存10 d肌苷酸质量比呈上升态势未出现最大值,其余5种鱼类肌肉组织肌苷酸质量比均在冻存第5天达到最大值。长时间的冻存将会降低食用鱼肉中肌苷酸质量比,导致鲜味下降,但经1周左右短时间的冻存后可明显提高鱼肉中肌苷酸质量比,增加鲜味,提高食用价值。     

双水相萃取法富集分离螺旋藻藻蓝蛋白的研究

以PEG／硫酸钠双水相体系,经一次萃取从钝顶螺旋藻（Spirulina platensis）细胞破碎液中富集分离藻蓝蛋白。结果表明,萃取最适宜的条件为12％PEG4000,15％Na2SO4,1％KCl,藻蓝蛋白收率为91．2％,分配系数达到8．01,分离因数达到6．33。对于螺旋藻藻蓝蛋白的富集分离,双水相萃取法与传统的盐析沉淀法相比,具有节省操作时间、简化操作过程、降低能耗和成本以及易于工艺放大等优点。     

Fe对眼点拟微球藻的生长和脂肪酸组成的影响

研究了铁对眼点拟微球藻(Nannochloropsis oculata)的生长和脂肪酸组成的影响.结果表明: Fe2 浓度在0～0.5 mmol/L的范围内,细胞比增长速率随Fe2 浓度的升高而降低.与对照组相比,随着Fe2 浓度的升高,细胞中主要的饱和脂肪酸和单不饱和脂肪酸占总脂肪酸的比例有所降低,而多不饱和脂肪酸占总脂肪酸的比例则有较大的提高.另外,在一定范围内(0～0.3 mmol/L),Fe2 浓度的升高则会促使细胞内积累二十碳五烯酸(EPA).细胞生长和EPA积累的最适Fe2 浓度是0.05 mmol/L,在这一浓度下细胞比增长速率为0.236±0.020 d-1,细胞中EPA质量分数比对照组提高约24%.     

莱州湾南岸第三陆相层年龄的铀系等时线法（TSD）测定

于莱州湾南岸ZK32-1钻孔第三陆相层上下边界处选取自生钙质结核,采用样品全溶铀系等时线法(TSD法)测定钙质结核的年龄。测试结果表明,所有等时线上的数据都有很好的线性关系,表明所获得的年龄数据是合理的。通过对样品代表性的讨论,说明所选取的钙质结核的年龄确可代表其所在地层的年龄,从而得出莱州湾南岸第三陆相层的沉积起止时间为72.16ka B.P.±7.41ka B.P.到45.34ka B.P.±5.35ka B.P.的结论。本研究首次获得莱州湾南岸地层的铀系TSD年龄,为全面认识该地区晚更新世地层年代学提供了一个新视角。     

应用MODIS影像监测海州湾无机氮浓度的研究

利用连云港海州湾2004年至2006年的水质监测资料,选取时间上完全同步的空间分辨率为500m的MODIS Terra 1B数据,对反射率的单波段因子和波段组合因子与可溶无机氮（DIN）质量浓度之间进行相关分析。从总体上看,单波段因子与DIN质量浓度的相关性较低,但在含氮基团倍频和合频吸收带附近的波段（波段2、7）反射率与DIN质量浓度呈负相关,显示了含氮基团对水体光谱特征的影响;在波段组合因子中,因子F11（3.4）和F13（3.4）与DIN质量浓度呈显著正相关,用这两个因子建立DIN质量浓度的回归模型,R^2都达到0．7以上,相对精度达70％左右,最终选择因子F11（3.4）的线性模型反演该海域的DIN质量浓度,其结果与实际情况非常吻合。     

晚更新世以来古气候与海平面变化在东海地区的响应

第四纪特别是晚更新世以来气温的大幅度冷暖变化,导致全球海平面的变化,引起陆架海侵和海退。东海陆架的气候和海平面变化与全球变化基本同步。晚更新世以来,东海陆架随全球气候变化发育多次海进海退旋回。东海陆架上的古河道、古三角洲和潮流沙脊等沉积体系的形成和演化与海平面升降变化存在耦合关系。     

栉孔扇贝不同组织SOD、CAT和MPO活力的比较分析

对2龄和3龄栉孔扇贝外套膜、鳃、消化盲囊、肾、闭壳肌、性腺组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和髓过氧化物酶(MPO)活力分析表明,检测组织均存在这三种酶,且在不同组织中活力表现存在明显差别,其中肾组织SOD、CAT活力最高,而MPO在消化盲囊中表现最高活力,与其它组织相比均呈显著性差异(P＜0.05).不同贝龄的栉孔扇贝酶活力比较表明,三龄贝各组织SOD、CAT和MPO活力均高于二龄贝.