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Diagnosis of iridovirus in large yellow croaker by PCR 总被引:1,自引:0,他引:1
A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection. 相似文献
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本文对黄海测区空气微生物作了一次测定 ,结果表明空气中海洋性细菌、真菌的检出率分别为 52 .4 %和 4 7.6 %。陆源性细菌、真菌的检出率分别为80 .0 %和 6 0 .0 %。陆源性微生物出现机率大于海洋性的。空气微生物以细菌为主。测区平均的海洋性空气细菌、真菌、总菌量及真菌 /总菌 %分别为 592 .6 ,32 9.1 ,92 1 .7CFU·m-3 及 35.4。平均的陆源性空气细菌、真菌、总菌量及真菌 /总菌 %分别为 6 89.1、377.9、1 0 6 7.0CFU·m-3 及 35.7。文章分析了空气微生物的时空分布状态。指出测区空气微生物状态反映了海 -气、海 -陆、人和自然的相互作用。 相似文献
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Daoyong ZHANG Xiangliang PAN Shuimu LIAO Chenglin HUANG Jingmei ZHANG 《中国地球化学学报》2006,25(B08):133-133
Low molecular weight organic acids (LMWOAs) are important components of root exudates. They play an important role in immobilizing and remobilizing contaminants in rhizospheric soil. Effects of four LMWOAs on the sorption and desorption behavior of p-chlorophenol by yellow earth was studied in batch mode. The results showed that the previous application of LMWOAs to enhanced adsorption capacity of p-chlorophenol of the soil in the order of maleic acid〉malic acid〉oxalic acid〉citric acid. However, when LMWOAs were applied to soil where p-chlorophenol had been previously adsorbed, substantial p-chlorophenol was desorbed from soil by oxalic acid, whereas citric acid, malic acid and maleic acid didn't desorb as much p-chlorophenol from soil as deionized water. 相似文献
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Monthly fishery survey data of the small yellow croaker Larimichthys polyactis in the southern Yellow Sea from2003 and 2013 were employed to evaluate the variation in the resource distribution and biological characteristics(especially body length and sex ratio) in the population on a decadal scale.The results indicated that the small yellow croaker migrated from the Shawai fishing ground to the Dasha fishing ground in spring and was mainly distributed in the central and western parts of the Dasha fishing ground in April and May.Larimichthys polyactis in the Dasha fishing ground migrated eastward to offshore wintering grounds in autumn and reached the central Dasha fishing ground in October and November.The small yellow croaker entered the western waters of the Shawai fishing ground in winter.A large number of age 0+ fish occurred in the Shawai and Jiangwai fishing grounds in October of 2003 and 2013.The body lengths of the spawning stock and wintering stock in 2013 were larger than those in 2003,and the monthly sex ratios(female to male) were significantly less than 1 in both years.The monthly distribution of this fish in the southern Yellow Sea was consistent with a previous finding that "the stock migrated between the wintering grounds in the west of Jeju Island and the Lüsi spawning grounds" but tended to move more northward,with the spawning grounds extending outward.In the past decade,body length variation experienced a decline after an increase,rather than a steady decrease.The sex ratio in the single-stick stow net showed a tendency to increase over the decade,but was either less than or more than 1 depending on the fishing gear;therefore,further studies should be conducted to determine the sex structure. 相似文献
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本文提出了一种通过调节一类状态依赖切换系统的阈值来生成多翅膀混沌吸引子的设计方案.首先,设计了两个类Lorenz子系统;然后,基于这两个子系统构造了一个状态依赖于阈值的切换策略,从而构造了一类状态依赖于阈值的切换系统;最后,通过调整切换系统的阈值来改变鞍焦平衡点的个数与分布,使得该切换系统能够产生4-6-8翅膀的混沌吸引子.此外,根据切换系统的状态方程设计了该系统的模拟电路,电路仿真结果与数值仿真结果一致.实验结果证实:该设计方案可以在不增加系统维数或无需设计复杂非线性函数的情况下生成多翅膀混沌吸引子. 相似文献
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小黄鱼是我国近海四种最重要的经济鱼类之一,在过去的几十年中小黄鱼种群及其两个地理亚种群经历了巨大的变化。小黄鱼的种群动力学研究,对于维持这一重要渔业的可持续管理是至关重要的。目前仅有的两个小黄鱼种群动力学模型只涵盖了较短的时间,且没有关注其空间差异。本文研究了1968年至2015年间黄渤海和东海小黄鱼的种群动力学模型,包含和不包含空间分层结构的两种贝叶斯模型被用于分析其种群动力学的大尺度空间异质性。本文分析了不同的假设,来研究小黄鱼种群动力学潜在的变化趋势。研究结果表明小黄鱼种群动力学特征具有明显的时间和空间变化。种群的增长速度从20世纪八十年代开始增加,而可捕系数从1981年到2015年增加了两倍多。与黄渤海亚种群相比,东海的小黄鱼亚种群生长速度更快,遭受的捕捞压力也更大。基于最大可持续产量MSY的参考点表明,无论是整个小黄鱼种群还是两个亚种群,近年来都有非常高的过度捕捞风险。因此我国小黄鱼的渔业管理急需更加保守的管理策略,同时考虑其地域差异。本文所用的方法可以应用于其他种类的资源评估和渔业管理,尤其是具有空间异质性和数据有限的种类。 相似文献