笠贝soxb、mox基因的鉴定及在足原基发育中的表达模式

足是软体动物最重要的器官之一,目前对其发育机制尚不了解。软体动物的足由神经和肌肉两部分构成,研究足部神经和肌肉系统在发育早期的的动态变化,对于了解足的形态建成机制具有重要意义。本文利用电镜研究了笠贝(Lottiagoshimai)足的早期发育过程。克隆了lgo-soxb和lgo-mox基因,整装原位杂交表明这两个基因分别表达在早期笠贝幼虫的神经外胚层和中胚层,可作为足发育过程的分子标记。不同发育阶段幼虫的原位杂交结果表明,神经外胚层和中胚层细胞在足发育的起始阶段尚未重叠,而在后续发育阶段,二者相向运动并重叠在一起。结果提示组成贝类足部的神经和肌肉细胞起源于早期胚胎不同的部位,在发育过程中逐渐互相接近并重叠,至担轮幼虫晚期共同作用形成足原基。本研究展示了足原基的早期形成过程,为深入研究软体动物足的发育机制提供了基础信息。     

紫刺参早期生长发育与体色形成的研究

本研究对紫刺参胚胎和幼体发育过程进行了显微观测,比较分析了其幼体早期生长情况与体色形成过程。结果表明:(1)在水温21.0±0.2℃条件下,紫刺参受精卵在受精10～15min后释放第一极体,5h 30min～6h 30min进入囊胚期, 18～20h发育成原肠胚; 30～34h进入耳状幼体阶段, 8～10d变态发育为樽形幼体, 12～14d发育成稚参;紫刺参胚胎和幼体发育时序与普通刺参无显著差异。(2)紫刺参早期发育中的樽形幼体发生率和附着变态率分别为58.4±4.7%和45.1±2.7%,高于普通刺参。(3)紫刺参浮游阶段后期随日龄增长表现出较普通刺参发育更快的优势,在日龄6～7d表现出显著差异(P0.05)。(4)紫刺参稚参在日龄35d前后自背部开始着色,50d～60d腹部开始着色,110d体表紫色转变近乎成参体色,完成变色过程。     

大西洋鲑在封闭循环水养殖模式下的生长和性腺发育研究

本研究探讨了在封闭循环水养殖模式下大西洋鲑(Salmosalar)的生长发育模式,对封闭循环水系统养殖的大西洋鲑重要生长数量性状进行生物学测量,并跟踪性腺发育状况。本研究自2012年1月通过封闭循环水系统进行大西洋鲑养殖,经过周年养殖,体质量平均达到1194.42g,体长平均达到417.00 mm,体高平均达到100.58 mm,体宽平均达到53.52 mm,少数个体在养殖10个月后性腺发育较快,性腺质量达到9.76 g,性腺指数达到1.31%。本研究的研究结果为建立大西洋鲑标准化循环水养殖系统积累资料。     

单环刺螠soxb基因的克隆及其在幼虫发育中的表达特征

SOXB作为重要的转录因子,参与了动物内胚层分化、消化道形成、神经细胞和感官细胞分化的调控。本研究利用RACE技术克隆了单环刺螠(Urechis unicinctus)2个soxb亚族基因soxb1(Uu-soxb1)和soxb2(Uu-soxb2)的cDNA全长序列,大小分别是1871 bp和2906 bp。在两个推导的氨基酸序列中均包含SOX家族的HMG-box结构和SOXB亚族蛋白特有的Group B homology序列,进化树分析它们分别与SOXB1和SOXB2聚类。原位杂交结果显示,Uu-soxb1和Uu-soxb2为母源表达基因;二者在担轮幼虫中的表达图式存在差异,其中早期担轮幼虫中Uu-soxb1 mRNA广泛分布于虫体中,Uu-soxb2 mRNA则集中于顶纤毛束基部和虫体后部(口后纤毛环之后),中期担轮幼虫中,Uu-soxb2阳性信号主要位于纤毛环及虫体的下半球;而Uu-soxb1 mRNA主要位于虫体下半球;之后的发育中二者的表达位点基本一致,即:晚期担轮幼虫中主要位于虫体后部,体节幼虫和蠕虫状幼虫中主要分布于消化道和体壁处。本研究结果表明,单环刺螠soxb在消化道发育过程中的表达模式与其他已报道动物类似,提示其在消化道发育中具有保守的功能。     

Development strategies for the sea cucumber industry in China

The sea cucumber, Apostichopus japonicus, is an important marine aquaculture species in China. After nearly thirty years of development, the production of A. japonicus has become commercially lucrative and successful. In this report, current advances in sea cucumber industry are addressed in terms of the basic biology, culturing methods, and health care bene?ts. Next, the challenges restricting development of the sea cucumber industry are discussed, including weaknesses in the basic biological research, the problem of germplasm degradation, environmental stress caused by global climate change, and food safety problems. Finally, several strategies are presented that might contribute to sustainable development of the sea cucumber industry. These strategies include advances in genome studies, behavioral studies, selective breeding, ecological culture technologies, reforms in food safety management, and the development of health care functions based on contemporary medical practices. Thus, our work provides new insights into how to explore the sustainable development of the sea cucumber industry in the future.     

Dynamic metabolite alterations of Portunus trituberculatus during larval development

A mass mortality often occurs from molting to the megalopa stage during the larval development of the swimming crab Portunus trituberculatus. Larvae with insuffcient nutrient accumulation during the zoeal stages are probably unable to develop into juvenile swimming crabs. However, the nutritional information such as the primary metabolites is scarce for P. trituberculatus larvae. The aim of this work is to obtain an insight into the metabolite traits of P. trituberculatus at early developmental stages. 1 H nuclear magnetic resonance spectroscopy coupled with multivariate data analysis was used to determine how the metabolite pro?les shift during larval development in P. trituberculatus. Our results show that the trend of total metabolites exhibited a rise from zoea 1 to zoea 3, followed by a drop from zoea 4 to megalopa and recovery during the ?rst juvenile stage. A large-scale depletion of total metabolites in the zoea 4 and megalopa stages suggests a deep depression of metabolic activity, which may be linked to the mass mortality from molting to the megalopa stage. These ?ndings provided essential metabolic information about the larval development of P. trituberculatus and important clues for understanding the nutritional requirements of P. trituberculatus in early developmental stages.     

A novel spatial index using spatial analyses and hierarchical fuzzy expert system for obtaining green TOD: a case study in Tehran city

Sustainable development is a vital and challenging factor for managing urban growth smartly. This factor contains three main components, namely economic growth, ecological protection and social justice. Green Transit-Oriented Development (GTOD) is a consummate planning approach in line with those components. Implementation of GTOD in an urban area is underpinned by its quantification. Therefore, a quantitative spatial index based on several indicators related to TOD and Green urbanism concepts should be developed. In this study, Geo-spatial Information Science and hierarchical fuzzy inference system (HFIS) were employed to calculate the indicators and aggregate them, respectively. In order to showcase the feasibility of the proposed method, it was implemented in a case study area in the City of Tehran, Iran. The result of this method is an integrated spatial GTOD index, which measures the neighbourhoods’ GTOD levels. These measurements specify weaknesses and strengths of neighbourhoods’ factors. Therefore, this index helps decision-makers to plan neighbourhoods based on land use and public transit views. Additionally, the HFIS method helps decision-makers to consider criteria and indicators with their inherent uncertainties and aggregate them with much fewer rules. For evaluating the results, the developed GTOD index was assessed with municipal action planning and attraction maps. According to the outcomes of the assessment, it is concluded that the proposed method is adequately robust and efficient for smart and sustainable urban planning.     

遥感监测下的渝东南小城镇镇区拓展特征探究

DUAN Songjiang     

我国自然资源、自然资源资产监测发展现状及问题分析

自然资源、自然资源资产监测是实现自然资源统一管理和确权的基础性工作。本文通过对自然资源、自然资源资产监测概念和内涵的研究,从行政监测、航天监测、航空监测、地表监测、地下矿产监测、水资源监测、海洋监测等角度介绍了我国自然资源监测研究及工作实践的方法和手段;并对科学界定自然资源内涵与分类及监测目录、国家自然资源监测网络整合与建设、自然资源监测时空框架构建等主要问题的解决提出了建议。     

雷达遥感六十年：四个阶段的发展

雷达遥感问世60年来已经历了4个阶段的发展,其在对地观测中的作用正日益凸显,已经广泛应用于不同领域。4个阶段分别是单波段单极化阶段,多波段多极化阶段,极化和干涉阶段,以及以双/多站或星座、高时序高分宽幅、3维成像为代表的新阶段。本文结合作者长期在雷达遥感领域的研究经历,总结和回顾了雷达遥感的阶段发展和具有里程碑式的代表性技术;从观测技术、数据处理和应用角度阐述了新阶段雷达遥感的发展趋势,以及雷达遥感与人工智能和大数据结合的思考;最后着眼未来,介绍了月基雷达对地观测平台的前瞻性研究。