人工饲料饲养的对虾肠道菌群和水体细菌区系的研究

对人工饲料饲养的凡纳滨对虾Litopenaeus vannamei成虾肠道菌群组成及其水体细菌区系组成进行了研究.分离纯化后的革兰氏阴性菌鉴定到种的水平.对虾肠道菌群主要由弧菌Vibrio spp.、希瓦氏菌属Shewnella spp.、嗜氢菌属Hydrogenophaga spp.、伯克霍尔德氏菌Burkholderia spp.、气单胞菌Aeromonas spp.、食酸菌Acidovorax spp.和芽孢杆菌Bacillus spp.组成.水体细菌区系主要包括弧菌Vibrio spp.、希瓦氏菌属Shewnella spp.、艾肯菌Empedobacter spp.、无色杆菌Achromobacter spp.和芽孢杆菌Bacillus spp..肠道菌群和水体细菌区系的优势菌是副溶血弧菌Vibrio parahaemolyticus和芽孢杆菌Bacillus spp..利用Shannon-Wiener指数进行多样性分析,发现虾肠道多样性指数（H）和均匀度（J）高于水体细菌区系,而丰度（D）较低.     

Cycladophora davisiana (Radiolaria) in the Okhotsk Sea: A key for reconstructing glacial ocean conditions

Cycladophora davisiana, a radiolarian species dwelling at mesopelagic depths, is known as a representative glacial fauna due to its unique distribution during glacial periods. In the present ocean, abundant production of C. davisiana is only observed in the Okhotsk Sea, indicating an adaptation of C. davisiana for seasonal sea-ice covered conditions. We found pronounced abundant production of C. davisiana during the early to middle Holocene in the Okhotsk Sea, suggesting more favorable conditions for C. davisiana than the present Okhotsk Sea. In order to clarify the reason, oceanographic conditions during the Holocene were reconstructed based on biomarkers, lithogenic grains including ice-rafted debris (IRD), biogenic opal, and total organic carbon (TOC) in two sediment cores from the Okhotsk Sea. These indicators suggest that the pronounced C. davisiana production may be attributed to: 1) a supply to mesopelagic depths under intensified stratification of fine organic particles derived from coccolithophorids, bacteria, and detrital materials; and 2) cold, well-ventilated intermediate water formation.     

Determination of phospholipid fatty acid structures and stable carbon isotope compositions of deep-sea sediments of the Northwest Pacific, ODP site 1179

Sediment samples ranging from 0.05 to 278 m below sea floor (mbsf) at a Northwest Pacific deep-water (5564 mbsl) site (ODP Leg 191, Site 1179) were analyzed for phospholipid fatty acids (PLFAs). Total PLFA concentrations decreased by a factor of three over the first meter of sediment and then decreased at a slower rate to approximately 30 mbsf. The sharp decrease over the first meter corresponds to the depth of nitrate and Mn(IV) reduction as indicated by pore water chemistry. PLFA-based cell numbers at site 1179 had a similar depth profile as that for Acridine orange direct cell counts previously made on ODP site 1149 sediments which have a similar water depth and lithology. The mole percentage of straight chain saturated PLFAs increases with depth, with a large shift between the 0.95 and 3.95 mbsf samples. PLFA stable carbon isotope ratios were determined for sediments from 0.05 to 4.53 mbsf and showed a general trend toward more depleted δ¹³C values with depth. Both of these observations may indicate a shift in the bacterial community with depth across the different redox zones inferred from pore water chemistry data. The PLFA 10me16:0, which has been attributed to the bacterial genera Desulfobacter in many marine sediments, showed the greatest isotopic depletion, decreasing from − 20 to − 35‰ over the first meter of sediment. Pore water chemistry suggested that sulfate reduction was absent or minimal over this same sediment interval. However, 10me16:0 has been shown to be produced by recently discovered anaerobic ammonium oxidizing (anammox) bacteria which are known chemoautotrophs. The increasing depletion in δ¹³C of 10me16:0 with the unusually lower concentration of ammonium and linear decrease of nitrate concentration is consistent with a scenario of anammox bacteria mediating the oxidation of ammonium via nitrite, an intermediate of nitrate reduction.     

Sustainable shellfish aquaculture in Saldanha Bay,South Africa

The carrying capacity for bivalve shellfish culture in Saldanha Bay, South Africa, was analysed through the application of the well-tested EcoWin ecological model, in order to simulate key ecosystem variables. The model was set up using: (i) oceanographic and water-quality data collected from Saldanha Bay, and (ii) culture-practice information provided by local shellfish farmers. EcoWin successfully reproduced key ecological processes, simulating an annual mean phytoplankton biomass of 7.5 µg Chl a l^–1 and an annual harvested shellfish biomass of about 3 000 tonnes (t) y^–1, in good agreement with reported yield. The maximum annual carrying capacity of Small Bay was estimated as 20 000 t live weight (LW) of oysters Crassostrea gigas, or alternatively 5 100 t LW of mussels Mytilus galloprovincialis, and for Big Bay as 100 000 t LW of oysters. Two production scenarios were investigated for Small Bay: a production of 4 000 t LW y^–1 of mussels, and the most profitable scenario for oysters of 19 700 t LW y^–1. The main conclusions of this work are: (i) in 2015–2016, both Small Bay and Big Bay were below their maximum production capacity; (ii) the current production of shellfish potentially removes 85% of the human nitrogen inputs; (iii) a maximum-production scenario in both Big Bay and Small Bay would result in phytoplankton depletion in the farmed area; (iv) increasing the production intensity in Big Bay would probably impact the existing cultures in Small Bay; and (v) the production in Small Bay could be increased, resulting in higher income for farmers.     

Artificial Upwelling of Deep Seawater Using the Perpetual Salt Fountain for Cultivation of Ocean Desert

Deep seawater in the ocean contains a great deal of nutrients. Stommel et al. have proposed the notion of a “perpetual salt fountain” (Stommel et al., 1956). They noted the possibility of a permanent upwelling of deep seawater with no additional external energy source. If we can cause deep seawater to upwell extensively, we can achieve an ocean farm. We have succeeded in measuring the upwelling velocity by an experiment in the Mariana Trench area using a special measurement system. A 0.3 m diameter, 280 m long soft pipe made of PVC sheet was used in the experiment. The measured data, a verification experiment, and numerical simulation results, gave an estimate of upwelling velocity of 212 m/day. This revised version was published online in July 2006 with corrections to the Cover Date.     

Seasonal variations of water system distribution and flow patterns in the southern sea area of Hokkaido,Japan

Hydrographic data and composite current velocity data (ADCP and GEK) were used to examine the seasonal variations of upper-ocean flow in the southern sea area of Hokkaido, which includes the “off-Doto” and “Hidaka Bay” areas separated by Cape Erimo. During the heating season (April–September), the outflow of the Tsugaru Warm Current (TWC) from the Tsugaru Strait first extends north-eastward, and then one branch of TWC turns to the west along the shelf slope after it approaches the Hidaka Shelf. The main flow of TWC evolves continuously, extending eastward as far as the area off Cape Erimo. In the late cooling season (January–March), part of the Oyashio enters Hidaka Bay along the shallower part of the shelf slope through the area off Cape Erimo, replacing almost all of the TWC water, and hence the TWC devolves. It is suggested that the bottom-controlled barotropic flow of the Oyashio, which may be caused by the small density difference between the Oyashio and the TWC waters and the southward migration of main front of TWC, permits the Oyashio water to intrude along the Hidaka shelf slope.     

Interstitial water chemistry of the inner continental shelf sediments off the gironde estuary

The interstitial water composition ( , alkalinity, Ca²⁺, Mg²⁺, Sr²⁺, Na⁺, K⁺) and the cation exchange capacity (CEC) were determined for the muddy sediments of the continental shelf off the Gironde Estuary (France), in the area where the sediment represents the deposit of the muddy suspension of the river. In comparison with seawater concentrations, the pore waters below 10 cm depth, show depletions of and Ca²⁺ and below a 30 cm depth show depletions of Mg²⁺. Inversely, the upper 10 cm an enrichment of Ca²⁺ concentration, and an increase of K⁺ concentration to a 40 cm depth. High values of are observed at the top 4 cm. Alkalinity enrichment is observed along the length of the core. Applying the alkalinity models for the sediment below a 10 cm depth demonstrates generally that calculated alkalinities are higher than the measured ones. Ca²⁺ dissolution occurs at the first 10 cm and authigenic carbonate precipitation starts beneath that level. Mg²⁺ depletion is accompanied by bicarbonate loss. This proves that Mg²⁺ depletion is due to a Mg-silicate reaction. The result of the CEC does not confirm the Mg²⁺ uptake by clay minerals in exchangeable site, under reducing conditions. Diffusion and bioturbation play an important role in the pore water concentration at the top of the core.     

呼伦湖的近期扩张及其与全球气候变化的关系

通过近２０多年湖泊水量平衡分析发现，湖泊水位变化主要由湖盆内径流补给量的丰枯决定。进一步分析揭示，呼伦湖地区乃至整个东北地区，本世纪以来随气温升高，随水有增加的趋势。降水增加导致入湖径流量，湖水位上升，呼伦湖本世纪以来的扩张与内蒙古东部地区其他内陆湖泊的变化一致，但这在我国乃至整个亚洲内陆干旱或半干旱区是独一无二的，为此成为这一地区气候变化的指示器。     

基于规则波波形的超声波非接触测量有关问题探讨

波浪波形的非接触测量近年来获得广泛的应用。本文以规则波为讨论对象，针对介质为空气的超声波测量方式进行波形测量误差分析，并结合实验室和实船试验进行对比分析。理论分析表明：在规则波取为坦谷波和ＳＴＯＫＥＳ波的前提下，超声波技术几乎产生相同的最大误差。定量分析表明，在对测量精度限制的条件下，对文中提及的各项参数应作有效的控制     

应用卫星遥感边缘效应解译冬季黄海的水团配置

作者提出叶绿素是海洋浮游植物生物量的一个重指标,在两水系交汇的锋区,由于浮游植物大量繁殖,叶绿素富集,处于高生产力状态。同步获取黄海叶绿素在富集带及邻近外区的分布,可以确定水团边界。对此,作者根据卫星图象经几何纠正后,通过与黄海叶绿素a的边缘效应信息及区域分布态势比较并解译,将冬季黄海的水团划分为八种。本文还引证了遥感与其它常规资料佐证。