移动轨迹数据可视化方法总结

作为移动社交网络的主体,人们移动带来的位置轨迹不仅记录了人的行为历史,也记录了人与社会的交互活动信息。移动社交网络中位置轨迹数据的分析与利用为解决城市问题提供了一种新的思路。本文概述了轨迹数据可视分析中的几种方法,总结了轨迹数据可视分析研究中存在的问题和面临的挑战。     

2010-2018年北极夏季中国北极科学考察航行期间被动微波遥感海冰密集度与船基目视观测资料的比较

为了更有效地将卫星数据应用于北极航行导航,被动微波（PM）产品的海冰密集度（SIC）与从中国北极科学考察中收集到的船基目视观测（OBS）资料进行了比较。在2010、2012、2014、2016和2018年的北极夏季总共收集了3667组目测数据。PM SIC取自基于SSMIS传感器的NASA-Team（NT）、Bootstrap（BT）以及Climate Data Record（CDR）算法和基于AMSR-E/AMSR-2传感器的BT、enhanced NT（NT2）以及ARTIST Sea Ice（ASI）算法。使用PM SIC的日算术平均值和OBS SIC的日加权平均值进行比较。比较了PM SIC和OBS SIC之间的相关系数,偏差和均方根偏差,包括总体趋势以及在轻度/普通/严重冰况下的情况。使用OBS数据,浮冰尺寸和冰厚对不同PM产品SIC反演的影响可以通过计算浮冰尺寸编码和冰厚的日加权平均值来评估。我们的结果显示相关系数的范围为0.89（AMSR-E/AMSR-2 NT2）到0.95（SSMIS NT）,偏差的范围为-3.96%（SSMIS NT）到12.05%（AMSR-E/AMSR-2）,均方根偏差的范围为10.81%（SSMIS NT）到20.15%（AMSR-E/AMSR-2 NT2）。浮冰尺寸对PM产品的SIC反演有显著的影响,大多数PM产品倾向于在小浮冰尺寸情况下低估SIC,而在大浮冰尺寸情况下高估SIC。超过30 cm的冰厚对于PM产品的SIC反演没有明显影响。总体来看,在北极夏季,SSMIS NT SIC与OBS SIC之间有着最好的一致性,而AMSR-E/AMSR-2 NT2 SIC与OBS SIC的一致性最差。     

页岩岩相分类表征及对建产区产能的影响

单井产气能力差异大,导致持续稳产困难是页岩气建产区面临的关键难题.围绕页岩气建产区特点,建立了考虑建产区产气能力影响的优质海相页岩岩相分类表征技术流程.首先,分析了建产区优质页岩产气能力差异主控因素,制定了建产区优质页岩岩相分类方案;然后,建立了TOC含量,孔隙度,泥质、硅质、钙质矿物含量等关键参数测井计算模型,结合页...     

Micrometer measurements of visual double stars at Calar Alto

We present 93 micrometer measurements of 55 double stars observed with the 152 cm telescope at the Spanish observatory at Calar Alto (Almeria, Spain).     

Purification and Characterization of a Novel Hydrolase That Can Specifically Degrade the Polysaccharide Isolated from Green Seaweed Ulva prolifera

The extracellular polysaccharide hydrolase-producing strain EP-1 was isolated from seawater and identified as Paenibacillus pabuli. Furthermore, a homogeneous extracellular polysaccharide hydrolase from Paenibacillus pabuli EP-1 was purified by combining ion-exchange chromatography and size exclusion chromatography with a purification fold of 90.69 and recovery of 16.23%. Characterization of the purified polysaccharide hydrolase revealed a molecular mass of 38 k Da and optimum activity at 45℃ and pH 6.0. The polysaccharide hydrolase maintained its stability within a wide range of pH(3.0–12.0) and thermal stability when the temperature was below 50℃. The presence of Hg²⁺, Fe²⁺, Mn²⁺, Co²⁺ and SDS notably decreased hydrolase activity, and organic solvents such as formaldehyde, acetone, DMF and acetonitrile completely inhibited hydrolase activity. The purified hydrolase had no activity on agar, carrageenan, gellan gum, sodium alginate, or starch, but effectively hydrolyzed the polysaccharide from Ulva prolifera. The Km and Vmax values of this hydrolase were 43.84 mg m L^-1 and 4.33 mg m L^-1 min^-1, respectively. The sequence analysis with quantitative time-of-flight mass spectrometry indicated that the hydrolase was an endoglucanase.     

海洋微塑料检测技术研究进展

海洋微塑料污染目前已成为全球性的环境问题,近年来海洋微塑料检测技术越来越受到人们的关注。本文简要总结了微塑料样品采集及处理方法,并从检测方法的角度系统综述了近些年来微塑料检测技术的研究进展,涉及目视分析法、光谱法(如傅立叶变换红外光谱法和拉曼光谱法)、热分析法以及其他分析方法等(如质谱法以及扫描电子显微镜-能谱仪联用法)。本文简要评价了各类检测方法的优缺点,以期为海洋微塑料检测技术的发展提供参考,并探讨了未来海洋微塑料检测技术的发展方向。     

Isolation and Characterization of Collagen from Squid(Ommastreoges bartrami) Skin

Collagen of squid (Ommastrephes bartrami) skin was examined in the present study. Histology showed that collagen fiber in the skin was partially cross-linked with muscle fiber. Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were extracted from the skin and characterized. The results of amino acid composition and electrophoretic patterns revealed that ASC and PSC were both type Ⅰ collagen, containing α1 and α2 chains. FTIR (fourier transform infrared spectroscopy) investigations con-firmed the existence of helical arrangements in PSC of squid skin. The denaturation temperature (Td) and shrinkage temperature (Ts)of PSC were 29.4℃ and 52.8℃, respectively.     

Isolation and Characterization of Collagen from Squid(Ommastrephes bartrami) Skin

Collagen of squid(Ommastrephes bartrami) skin was examined in the present study. Histology showed that collagen fiber in the skin was partially cross-linked with muscle fiber. Acid-solubilized collagen(ASC) and pepsin-solubilized collagen(PSC) were extracted from the skin and characterized. The results of amino acid composition and electrophoretic patterns revealed that ASC and PSC were both type Ⅰ collagen,containing α1 and α2 chains. FTIR(fourier transform infrared spectroscopy) investigations confirmed t...     

极地产适冷脂肪酶菌株的酶学性质研究

从南北极环境样品中分离到7株产脂肪酶的细菌,经16SrDNA序列分析表明,这些细菌分别属于假交替单胞菌属（Pseudoalteromonas）和嗜冷杆菌属（Psychrobacter）．采用p-NPP法对这7株细菌所产的脂肪酶进行酶学性质研究,结果显示这些酶的最适作用温度在30～40cC、最适作用pH值在7—8之间,在低温下能保持较高的剩余活力,对热敏感,属于适冷脂肪酶．其中假交替单胞菌（Psychrobacter sp．7342）所产脂肪酶具有低温下酶的剩余活力高、有效作用温度和pH范围广、热稳定性较好及对多种金属离子抗性强等特点．该菌株能利用多种单一氮源和碳源产酶,最适产酶温度为25℃．在此基础上进行正交实验分析得到了该菌株的最佳发酵条件为：蛋白胨和淀粉含量各为1．33％,酵母膏含量为0．3％,温度为25℃．     

壳聚糖酶基因在解脂耶氏酵母中的表达及重组酶性质研究

为构建高效表达壳聚糖酶工程菌株,以Microbacteriumsp.基因组为模板扩增壳聚糖酶基因,目的基因与表达载体pINA1317连接构建重组质粒,重组质粒NotⅠ酶切线性化后转化解脂耶氏酵母Y.liPolytica Po1h。所得结果为PCR扩增得到约1000bp的特异性片段,序列分析表明含有壳聚糖酶全长基因,开放阅读框801bp,编码266个氨基酸残基。转化子酶活力为10.4U/mL,是原菌株酶活力的3.15倍。重组蛋白经DEAE-Sepharose FF分离纯化,达到电泳纯,SDS-PAGE显示单一条带,分子量约41kDa。重组酶反应最适温度为45℃,最适pH为5.6,Km和Vmax分别是0.926mg/mL和6.15U/mL。质谱分析酶解产物主要为三糖和五糖。实现了壳聚糖酶在解脂耶氏酵母中的分泌表达,为壳聚糖酶的工业化生产奠定了理论基础。