凯氏拟小球藻己糖激酶基因的克隆及其在不同培养条件下的表达分析

为研究凯氏拟小球藻(Parachlorellakessleri)糖代谢分子机制,本研究采用RT-PCR和RACE技术从凯氏拟小球藻中克隆了己糖激酶基因CkeHK(GenBankID:AHF54566),并对其自养、异养、混养条件下的转录表达进行分析。结果表明,该序列的cDNA全长为1844bp,开放阅读框1389bp,编码462个氨基酸。该蛋白的相对分子质量为49.73,等电点为6.98。实时荧光定量PCR结果显示,以自养培养条件为对照,异养培养和混养培养条件下,CkeHK均能够发生明显上调,且混养条件下上调量比异养条件下上调量更多,说明CkeHK可能在凯氏拟小球藻利用外源糖的过程发挥重要作用,并且光信号对于凯氏拟小球藻利用外源糖可能存在调控作用。这些研究结果为进一步阐明CkeHK的功能及其作用机制奠定了分子基础。     

倾角函数展开及其在分析法轨道预报中的应用

田谐项摄动是分析法轨道预报中的重要部分,其中包含大量倾角函数及其偏导数的计算.由于具有精度更高、速度更快的优点,倾角函数一般通过递推方法计算.以文献中提出的改进Gooding方法为基础,将其给出的程序稍加改进,在计算2–50阶倾角函数时缩短了约24%的计算时间.考虑到分析法预报过程中轨道平倾角变化很小,以泰勒展开式计算倾角函数,可极大提高计算速度,较大程度地减小分析法预报耗时,且引力场阶次越高,减小幅度越大,取50阶时预报耗时缩短了48%.另一方面,以2阶展开式计算倾角函数时,与改进Gooding法相比,分析法预报星历偏差很小.对于500 km高度的低轨卫星,分别以改进Gooding法和2阶泰勒展开式计算倾角函数,预报3天,当地球引力场阶次不高于50时,二者预报星历偏差RMS (Root Mean Square)低于1 mm,且随着轨道高度的增加,预报星历偏差RMS逐渐减小.     

水平分层弹性粘弹性介质的静力响应

本文首先利用传播矩阵法构造出了水平分层弹性介质在表载、体力及内部力源联合作用下的一般模型。然后利用“对应原理”将弹性问题的一般模型推广到粘弹性问题的一般模型——粘弹性分层介质传播矩阵基本方程。最后用粘弹性分层介质传播矩阵方程对一个实际地学问题做了试算,计算结果说明,用传播矩阵法处理粘弹性问题是非常有效的。     

基于扇形子镜的12m光学红外望远镜拼接共相误差仿真分析

为满足国内对应用大型通用光学望远镜在光学红外波段进行天文观测的需求, 中国计划建设一架12 m大口径光学红外望远镜(12-meter large aperture optical-infrared telescope, LOT). LOT对于中国天文界具有极其重要的意义, 将会在近红外波段实现共相. LOT的主镜将采用子镜拼接的方案, 由72块扇形子镜拼接而成, 并且中央部分有一块直径4.5m的圆形镜. 分析了采用扇形子镜拼接方案下LOT的共相误差, 提供了详细的共相误差理论推导过程, 并对包括3种子镜面外误差在内的误差源进行深入的单独模拟和综合模拟. 在共相波长为1μm的条件下, 当服从正态分布的倾斜误差的均方根值小于0.016^\prime\prime或服从正态分布的活塞误差的均方根值小于42.5nm时, LOT的斯特列尔比大于0.8. 仿真结果对于采用共相拼接镜面主动光学的主镜在LOT中的应用具有重要意义.     

Dynamically equivalent rocking structures

Predicting the rocking response of structures to ground motion is important for assessment of existing structures, which may be vulnerable to uplift and overturning, as well as for designs which employ rocking as a means of seismic isolation. However, the majority of studies utilize a single rocking block to characterize rocking motion. In this paper, a methodology is proposed to derive equivalence between the single rocking block and various rocking mechanisms, yielding a set of fundamental rocking parameters. Specific structures that have exact dynamic equivalence with a single rocking block, are first reviewed. Subsequently, approximate equivalence between single and multiple block mechanisms is achieved through local linearization of the relevant equations of motion. The approximation error associated with linearization is quantified for three essential mechanisms, providing a measure of the confidence with which the proposed methodology can be applied. Copyright © 2014 John Wiley & Sons, Ltd.     

Aeromagnetic study of peninsular India

The degree sheet Aeromagnetic maps up to 17‡N, acquired from the Geological Survey of India, have been manually redigitised at 6 minute intervals to study the long wavelength anomalies over peninsular India. These data have been collected at different survey altitude, epochs, flight line directions, etc. Great care has been taken to correct the total field map and remove the contribution due to the core field and prepare an accurate crustal anomaly map. For the first time, a regional map, depicting the NW-SE structural features north of the orthopyroxene isograd with the essentially E-W features to the south of it and revealing several well known structures, is presented. The analytical signal is calculated to delineate the source fields of these anomalies. It dramatically maps the charnockites and is able to delineate the orthopyroxene isograd. In the Dharwar region the magnetic signatures are associated with the intrusives/ iron ore bodies. Thus, we find that the source rocks of the aeromagnetic anomalies are the host province of charnockites in the SGT and the intrusives/iron ore bodies in the Dharwar belt. Gravity residuals are calculated and a tectonic map of the region is presented from the combined geopotential data.     

Evaluation of one-dimensional in situ leaching processes

A methodology to characterizing processes of in situ leaching is developed to investigate various parameters that may affect the recovery of a valuable mineral and assure a successful application of the in situ leaching technology. The governing equations of in situ leaching processes for the one-dimensional case are solved both analytically and numerically for both the consumption rate of a lixiviant and the production rate of a target mineral. A numerical simulator, which is developed to evaluate coupled effects among leaching kinetics, solution flow and transport of the dissolved mineral species in saturated ore deposits, is validated against the steady-state solutions and applied to investigate the transient effects of various parameters on the mineral recovery. Results from the evaluation indicate that there exists an optimal flow velocity range of leach solution for the effective leaching of a particular ore deposit. The determination of this optimal leaching velocity may become a key to the design of a real in situ leach mine. Results of the parameter sensitivity study illustrate the relative importance of other parameters such as porosity, ore grade, and reaction rate. Copyright © 1999 John Wiley & Sons, Ltd.     

桩体复合地基桩、土相互作用的解析法

桩体复合地基的设计中,承载力及沉降的计算一直没有较好地考虑桩、土和垫层的相互作用。根据三者的协同作用,推导出大面积群桩中桩、土的荷载传递基本微分方程并给出其解答。该方法提高了计算速度,土性参数均为常规指标,简便易得。该方法还考虑了桩侧阻随侧向土压力的变化以及垫层、土体压缩模量随压力的变化。与有限元计算结果对比表明了该解答的可行性。能够对桩土分担比以及沉降的计算提供帮助,是一种简便实用的方法。     

Ring foundation on elastic subgrade: an analytical solution for computer modelling using the Lagrangian multiplier method

In the practice of geotechnical engineering, the case of a ring footing carrying a set of concentrated point loads is a common problem. At times, the induced vertical and angular displacements for the ring footing need to be evaluated at a relatively precise level. By making use of the governing set of equations derived for the case of a general curved beam, expressions that can be easily implemented in modern computing software are derived for the vertical and angular displacements of a ring footing of rectangular cross section as functions of the radial position. The loading case considered is a vertical point load, and the soil is modelled as elastic. Estimates of the displacements have been shown for a common range of practical applications. The behaviour for a set of concentrated loads may be evaluated using the derived equations through direct superposition. Nonlinear finite element analysis is used to evaluate the vertical deflection and angular twist of the ring foundation. Numerical analysis performed for three ring foundations with different radii and cross sections is reported to validate the accuracy of the derived analytical solution. Copyright © 2016 John Wiley & Sons, Ltd.