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31.
等高线内插是提高地形图精度的一个常用手段,在地图综合等领域中有很重要的作用。在实际地形图数据库中,等高线除了存在完整的计曲线以外,还有一部分断裂的首曲线。已有的等高线内插研究中未考虑对此类数据的处理,为此,本文提出了一种基于Fréchet距离的断裂等高线内插算法。首先提出了等高线度量关系的计算方法,然后对等高线的节点均匀加密,并利用Fréchet距离进行相似度判断以选择参考等高线,最后根据“最近点”的方式进行插值。通过对江苏某地区实际数据的试验,验证了该算法的合理性,对于鞍部地区的内插,有更好的适应性和准确性。 相似文献
32.
The authors have previously determined that the effectiveness and failure pattern of the ice cover caused by flexural-gravity waves generated by a submerged body motion near the bottom ice can greatly depend on the depth of the water area. In its turn, the presence of a ledge on the ice surface may affect a wave propagation pattern. This paper presents an experimental study of the bottom contour influence on the deflection and length of flexural-gravity waves. The authors describe a numerical model for the analysis of the deformed state of ice caused by hydrodynamic loads due to a submarine motion, taking into account the bottom contour. The experiments are carried out in the ice tank. The results of calculations and experiments are compared. 相似文献
33.
The objective of this study is to efficiently extract detailed information about various man-made targets in oriented built-up areas using polarimetric synthetic aperture radar (POLSAR) images. This paper develops an improved approach for building detection by utilizing Two-Dimensional Time-Frequency (2-D TF) decomposition. This method performs outstandingly in distinguishing between man-made and natural targets based on the isotropic behaviors, frequency-sensitive responses, and scattering mechanisms of objects. The proposed method can preserve the spatial resolution and exploit the advantages of TF decomposition; specifically, the exact outlines of buildings can be effectively located, and more types of features (e.g., flat roofs, roads, and walls that are oblique to the radar illumination) can be distinguished from forests in complex built-up areas by 2-D TF decomposition. The coarser-resolution subaperture images that are produced in the azimuth direction, which correspond to different looking angles, are beneficial for detecting man-made structures with main scattering centers oriented at oblique angles with respect to the radar illumination. In the range direction, the obtained subaperture images, which correspond to various observation frequencies, can be helpful in distinguishing flat roofs and roads from forests. This method was successfully implemented to analyze both NASA/JPL L-band AIRSAR and L-band EMISAR data sets. The building detection results of the proposed method exhibit a significant improvement over those of other methods and reach an overall accuracy over 80%, with approximately 20% higher than the accuracies of K-means clustering and the entropy/alpha-Wishart classifier and approximately 10% higher than the accuracy of the support vector machine method. Moreover, building details can be precisely detected, obliquely oriented buildings can be identified, and the distinction between buildings and forests is significantly improved, as both visually and statistically indicated. This method is highly adaptable and has substantial application value. 相似文献
34.
随着点云在各个领域的广泛应用,对点云处理相关技术的研究更为活跃。目前,研究的热点主要集中在点云去噪、配准、分割、增强、特征提取和重建等方面。其中,特征提取起着承前启后的作用,具有十分重要的研究价值。基于面片裁切技术的点云轮廓线生成算法。首先,对点云数据进行面片提取,通过面片生长、融合生成特征面片,并根据提出的面片双向索引法快速构造特征线,最终实现点云平面轮廓线的生成。实验证明,本算法可快速、准确、有效地实现点云轮廓线的生成。 相似文献
35.
6种海洋致病性弧菌36kDa外膜蛋白特性分析 总被引:3,自引:0,他引:3
研究本着筛选和制备弧菌共同外膜蛋白亚单位疫苗,用十二烷基肌氨酸钠抽提并结合超速离心的方法提取了鳗弧菌(Vibrio anguillarum)、鱼肠道弧菌(V.ichthyoenteri)、溶藻弧菌(V.alginolyticus)、副溶血弧菌(V.parahaemolyticus)、哈维氏弧菌(V.harveyi)和创伤弧菌(V.vulnificus)6种海洋致病性弧菌的主要外膜蛋白, SDS-PAGE图谱比较发现这6种弧菌都存在36 kDa的外膜蛋白条带.通过电泳洗脱分离纯化这6种弧菌的36 kDa外膜蛋白,并制备了鳗弧菌菌株SJ060621的36 kDa外膜蛋白多抗.ELISA和Western-blot印迹结果显示,仅鳗弧菌SJ060621、S010610-1和哈维氏弧菌的36 kDa外膜蛋白存在免疫交叉反应;双向电泳分析了鳗弧菌SJ060621、S010610-1、哈维氏弧菌和溶藻弧菌的36 kDa外膜蛋白,2-DE图谱显示出鳗弧菌SJ060621和S010610-1均由3种等电点相近的蛋白组成,存在很高的同源性,2株鳗弧菌与溶藻弧菌和哈维氏弧菌在蛋白质组成数量和等电点上都存在较大的差异,结果说明通过主要外膜蛋白的分子量和免疫特性研究,可为筛选不同弧菌共同外膜蛋白的亚单位疫苗提供有利参考. 相似文献
36.
Dissolved proteins in seawater samples collected from a coastal area of Tokyo Bay, Sagami Bay and a location off the Kuroshio
Current were investigated by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and high
resolution two-dimensional electrophoresis (2-DE). Four to nine protein bands were detected in SDS-PAGE in the apparent molecular
weight (MW) range from 12 kilo Dalton (kDa) to 49 kDa. The 2-DE technique distinguished 10 to 46 protein spots exhibiting
isoelectric point (pI)/MW ranging 4.3–9.2/12–63 kDa. The elecrophoretic patterns were similar between the coastal and pelagic samples, as well
as previously reported patterns from various pelagic areas. The close similarity of electrophoretic mobility on both SDS-PAGE
and 2-DE gels indicates the compositional homogeneity of dissolved proteins in seawater throughout a broad range of marine
environments. Proteinaceous dissolved organic matter (DOM) that was unresolved and smeared staining characteristics on both
SDS-PAGE and 2-DE gels was first observed in Tokyo Bay waters in the present study and its possible sources are discussed.
Although the two protein species, 48 kDa and 39 kDa proteins, have been identified as homologues of Porin P and low molecular
weight-alkaline phosphatase of Pseudomonas aeruginosa PAO1, respectively, four strains of P. aeruginosa and two species of Pseudomonas spp. have been newly identified as the source organisms of these proteins using the N-terminal amino acid sequence data determined in previous studies. 相似文献
37.
Steven R. Beaupr Ellen R.M. Druffel 《Deep Sea Research Part I: Oceanographic Research Papers》2009,56(10):1717-1726
This study extends the 1991-1995 records of marine dissolved organic carbon (DOC) concentrations and Δ14C values at hydrographic Station M (34°50′N, 123°00′W) with new measurements from a frozen (-20 °C) archive of samples collected between April 1998 and October 2004. The magnitudes and synchronicity of major Δ14C anomalies throughout the time-series imply transport of DOC from the surface ocean to depths of at least 450 m on the timescale of months. Keeling plots of all measurements at Station M predict a continuum of possible background DOC compositions containing at least 21 μM of -1000‰ (i.e., ≥57,000 14C years) DOC, but are more consistent with mean deep DOC (38 μM, -549‰; i.e., 6,400 14C years). These results and coral records of surface dissolved inorganic carbon (DIC) Δ14C were used to estimate pre-bomb DOC Δ14C depth profiles. The combined results indicate that bomb-14C has penetrated the DOC pool to depths of ≥450 m, though the signal at that depth is obscured by short-term variability. 相似文献
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