培育密度和投饵量对长牡蛎壳黑选育品系幼虫生长存活及附着变态的影响

为探究长牡蛎(Crassostrea gigas)壳黑选育品系规模化繁育适宜的培育密度和投饵量,本研究以长牡蛎野生群体幼虫为对照组,分析了不同培育密度(3、7和15个/mL)和不同初始投饵量(0.4、0.8和1.6×10~3cells·ind^-1·d^-1)对长牡蛎壳黑选育品系幼虫生长、存活及附着的影响。研究显示,培育密度和投饵量对长牡蛎幼虫的生长速率、存活率和附着率有显著交互作用(P<0.05)。在培育密度3和7个/mL实验组,长牡蛎幼虫生长速率随投饵量增加而显著增大(P<0.05),并在培育密度7个/mL、投饵量1.6×10~3cells·ind^-1·d^-1实验组达到最大值。而在培育密度15个/mL实验组,投饵量增加对长牡蛎幼虫的生长速率无显著影响(P> 0.05)。且在培育密度15个/mL、投饵量1.6×10~3cells·ind^-1·d^-1实验组长牡蛎壳黑品系幼虫存活率和附着率最低。但在培育密度15个/mL实验组长牡蛎壳黑选育品系幼虫的壳高始终高于野生群体幼虫。且除投饵量0.4×10~3cells·ind^-1·d^-1实验组外,长牡蛎壳黑选育品系幼虫生长速率、存活率和附着率均高于野生群体幼虫。研究结果表明,长牡蛎壳黑选育品系幼虫最适培育条件为培育密度7个/mL、投饵量1.6×10~3cells·ind^-1·d^-1,增加投饵量可促进长牡蛎幼虫生长,但过高的培育密度会与投饵量产生拮抗作用,抑制幼虫生长,不利于幼虫存活和附着。与长牡蛎野生群体相比,壳黑选育品系幼虫能适应更高的培育密度环境,在饵料充足的条件下,其幼虫的生长、存活及附着性状均优于野生群体。     

Opportunistic-tended life history traits of Siniperca kneri in the Three Gorges Reservoir,China: potential responses to impoundment

Reservoir impoundment changes original fluvial habitats into lentic environments. Fish species adapted to lentic habitats may take the advantage of such habitat changes and show typical life history traits might facilitate the population increase. Siniperca kneri rarely occurred in fishery landing in the Three Gorges Reservoir (TGR) area before its impoundment in 2003, but it is now a dominant species. In this study, a total of 438 females of S . kneri were collected monthly during September 2012 through January 2014 using gill nets in the TGR. The age, growth, and reproductive biology were then investigated, and compared with other S . kneri populations. The standard length at age 1 was 149.9 mm in the TGR, which is larger than the three compared populations (i.e., Sandaohe Reservoir, Xinfengjiang Reservoir, and North River), but smaller than one (the Guishi Reservoir). The youngest mature female in the TGR was age 1, which is younger than that of the two compared populations (i.e., Xinfengjiang Reservoir and the North River). The relative fecundity in the TGR was 140 eggs/g, which is higher than that of the only available compared population (Xinfengjiang Reservoir, 96 eggs/g). Our results demonstrated that S . kneri in the TGR tended to have faster growth in the first year, a younger age at first maturation, and higher reproductive eff ort. We suggest that such opportunistic-tended life history traits might facilitate rapid increase of the population.     

Effects of stocking density on the growth and immunity of Atlantic salmon salmo salar reared in recirculating aquaculture system(RAS)

Atlantic salmon reared in recirculating aquaculture system(RAS) may lead to inappropriately high stocking density, because ?sh live in a limited space. Finding the suitable stocking density of Atlantic salmon reared in RAS is very important for RAS industry. In this paper, the in?uence of stocking density on growth and some stress related physiological factors were investigated to evaluate the ef fects of stocking density. The ?sh were reared for 220 days at ?ve densities(A: 24 kg/m^3; B: 21 kg/m^3; C: 15 kg/m^3; D: 9 kg/m^3 and E: 6 kg/m^3). The results show that 30 kg/m^3 might be the maximum density which RAS can af ford in China. The stocking densities under 30 kg/m^3 have no ef fect on mortality of Atlantic salmon reared in RAS. However, the speci?c growth rate(SGR), ?nal weight and weight gain in the high density group were signi?cantly lower than the lower density groups and middle density groups. Moreover, feed conversion rate(FCR) had a negative correlation with density. Plasma hormone T_3 and GH showed signi?cant decrease with the increase of the stocking density of the experiment. Furthermore, thyroid hormone(T 3), GH(growth hormone) activities were decreased with stocking density increase. However, plasma cortisol, GOT(glutamic oxalacetic transaminase) and GPT(glutamic pyruvic transaminase) activities were increase with stocking density increase. And the stocking density has no ef fects on plasma lysozyme of Atlantic salmon reared in RAS. These investigations would also help devise effcient ways to rear adult Atlantic salmon in China and may, in a way, help spread salmon mariculture in China.     

Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol

1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on marine organisms are still unknown. In order to provide a better understanding of the ecological risks of 1-PEA in marine environments, this study determined the toxic effects of 1-PEA on two marine diatoms (Phaeodactylum tricornutum and Skeletonema costatum). The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h. The contents of chlorophyll a, chlorophyll c, glutathione (GSH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured at the end of the exposure period. 1-PEA was shown to significantly inhibit the growth of diatoms, with 96-h EC50 values of 257.14 mg/Land 126.46 mg/L in P. tricornutum and S. costatum, respectively. In P. tricornutum, the levels of SOD, CAT, GPx, GSH, and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC50 value. However, in S. costatum, the activities of SOD and CAT, and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC50 value. Taken together, these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.     

水下收缩裂隙形成过程及裂缝充填模式研究

通过野外实地考察测试,对水下收缩裂隙整体形成过程、裂隙内充填物沉积模式、影响因素等进行了详细讨论,并且建立了相应的地质理论模型。水下收缩裂隙的形成共分4个阶段,依次为:泥水混合物进入低洼地带的初始混浊状态;沉积压实稳定阶段;水位线下降,盐度增大,裂隙形成阶段;沉积裂隙充填阶段。其中“异常高压作用”,对于初始裂隙的形成、沉积物孔隙水的有效排出以及主要渗流通道的发育起到了重要作用。单一泥质地层裂隙内充填物沉积模式与沙泥互层的多旋回地层略有差异,主要表现为沙层的润滑作用以及对早期裂隙内充填物类型的影响。上述两种地层裂隙内充填物沉积特征均与后期充注期次相对应,表现出很好的韵律性。裂隙纵剖面中生物发育层的出现,对裂隙内充填物的物理、化学性质将产生重要影响。     

马氏珠母贝生长性状的相关分析

对马氏珠母贝(Pinctadamartensi)不同生长时期的壳长、壳高、壳宽和活体质量进行了一年多的跟踪测量。分析表明,在不同生长时期,其4个性状的两两间的相关系数均达到极显著水平(P<0.01),但其相关系数大小排列顺序存在差异,8次测量的平均相关系数大小依次为壳高-壳长>壳宽-体质量>壳高-体质量>壳长-体质量>壳长-壳宽>壳高-壳宽。在不同生长时期,活体质量对壳高、壳宽的偏回归系数均极显著(P<0.01),而对壳长的偏回归系数在第1,3,7,15个月时不显著(P>0.05)。该研究的结果可为珍珠贝的选育种的重要目标性状的确定提供理论指导。     

营养盐硅和水温影响浮游植物的机制

通过研究分析Si和水温对浮游植物生长的变化和其集群结构的改变影响,探讨了硅和水温影响浮游植物生长的变化和其集群结构的改变,本文研究发现,浮游植物生长的变化和其集群结构的改变的过程,营养盐硅和水温影响浮游植物生长变化和其集群结构改变的机制,确定了营养盐硅和水温是海洋生态系统的健康运行的动力。     

柴达木盆地东缘祁连圆柏轮宽序列标准化的方法研究

利用在青海柴达木盆地东缘山地获取的大量祁连圆柏树轮资料,对拟合并去除树木生长趋势即标准化方法进行探讨,提出了一种总体曲线方法。该方法用包含完整树木髓心、并且在40~60年间达到生长顶峰的树轮资料拟合柴达木盆地东缘祁连圆柏的总体生长趋势曲线,并用广义的负指数函数来描述树木自茎的次生生长开始以来的径向生长过程。用相同的树轮资料建立估算缺失轮数的最初径向生长模型,其方差解释量高达90.9%。建立年表时包含髓心的样芯认定生理年龄为1年,不包含髓心的样芯用最初径向生长模型估算缺失轮数,然后全部样芯用生长趋势曲线对应部分进行去趋势标准化。该方法对建立可靠、准确的长年表有重要意义,所建年表比用传统负指数函数方法建立的年表保留了更多的低频变化信息。     

祁连山中部祁连圆柏生长与更新方式的树轮记录

选择祁连山中部50m×15m的祁连圆柏样方进行树轮生态学取样与分析。分析结果表明,样方内大部分祁连圆柏的树龄小于250年,最近几年有大量幼苗萌生。祁连圆柏的径生长方式各有异同,大部分祁连圆柏在生长过程中出现1次以上生长释放事件,1970¹980年间出现生长释放的频率最高,占总释放次数的35%,轮宽年表也显示祁连圆柏的轮宽指数自1970s以来持续上升。另外,相关分析结果表明,温度是影响采样点区域祁连圆柏径向生长的主要环境因子,较高的温度有利于树木生长,6、7月份的降水也与树木径向生长呈显著正相关。在全球变暖的背景下,如果能积极开展植树造林,并有效控制人类活动的干扰,该区域祁连圆柏林更新状况将会显著改观。     

Mesozoic Bimodal Volcanic Suite in Zhalantun of the Da Hinggan Range and Its Geological Significance： Zircon U-Pb Age and Hf Isotopic Constraints

1 Introduction Mesozoic volcano-intrusive rocks are widely distributed in the Da Hinggan Range of northeastern China, and are considered as one of the most spectacular geological sights in eastern Asia. Recently, studies on granites with high εNd(t) values and Phanerozoic crustal growth in the Centra Asian Orogenic Belt have greatly promoted fundamental research into the geology of this area (Jahn et al., 2000, 2001, 2004; Wu et al., 2000, 2002, 2003). However, work on the eruption time,…