海州湾4种鱼类生长特征的空间异质性

传统的渔业资源评估均假设鱼类的生长参数是匀质的,然而近年来越来越多的研究表明海洋鱼类生长存在空间异质性。为探究海州湾鱼类生长参数的空间异质性现象,本研究分析了2013–2018年海州湾及其邻近海域方氏云鳚(Pholis fangi)、尖海龙(Syngnatus acus)、小黄鱼(Larimichthys polyactis)和赤鼻棱鳀(Thryssa kammalensis)的空间分布,使用电子体长频率分析方法结合Bootstrap重抽样方法估算了这4种鱼类的生长参数及其在深、浅水区域中的差异。结果显示,这4种鱼类生长参数均表现出一定的空间异质性,其中尖海龙和小黄鱼生长参数的空间异质性表现较为明显。这种差异可能是由于空间上的理化条件、群落结构以及物种本身洄游分布的差异而产生的。     

紫刺参早期生长发育与体色形成的研究

本研究对紫刺参胚胎和幼体发育过程进行了显微观测,比较分析了其幼体早期生长情况与体色形成过程。结果表明:(1)在水温21.0±0.2℃条件下,紫刺参受精卵在受精10～15min后释放第一极体,5h 30min～6h 30min进入囊胚期, 18～20h发育成原肠胚; 30～34h进入耳状幼体阶段, 8～10d变态发育为樽形幼体, 12～14d发育成稚参;紫刺参胚胎和幼体发育时序与普通刺参无显著差异。(2)紫刺参早期发育中的樽形幼体发生率和附着变态率分别为58.4±4.7%和45.1±2.7%,高于普通刺参。(3)紫刺参浮游阶段后期随日龄增长表现出较普通刺参发育更快的优势,在日龄6～7d表现出显著差异(P0.05)。(4)紫刺参稚参在日龄35d前后自背部开始着色,50d～60d腹部开始着色,110d体表紫色转变近乎成参体色,完成变色过程。     

大西洋鲑在封闭循环水养殖模式下的生长和性腺发育研究

本研究探讨了在封闭循环水养殖模式下大西洋鲑(Salmosalar)的生长发育模式,对封闭循环水系统养殖的大西洋鲑重要生长数量性状进行生物学测量,并跟踪性腺发育状况。本研究自2012年1月通过封闭循环水系统进行大西洋鲑养殖,经过周年养殖,体质量平均达到1194.42g,体长平均达到417.00 mm,体高平均达到100.58 mm,体宽平均达到53.52 mm,少数个体在养殖10个月后性腺发育较快,性腺质量达到9.76 g,性腺指数达到1.31%。本研究的研究结果为建立大西洋鲑标准化循环水养殖系统积累资料。     

海洋发光弧菌生长和发光条件的研究

海洋生物发光研究对渔业、环境监测等有着举足轻重的意义。根据从湛江东海岛海域沉积物样品中分离出的一株海洋发光弧菌(Vibrio sp.,编号为D2),对这株菌的生长和发光条件进行了初步研究。实验结果表明,海洋发光弧菌D2在pH7.0、温度35℃、NaCl浓度为2.0%时,生长状态最好;在pH5~6、温度20℃、NaCl浓度为3.0%、细菌密度OD600达0.08时,发光强度最高。     

Effects of salinity fluctuation amplitudes on growth, osmolarity, Na＋-K＋-ATPase activity and Hsp70 of juvenile Chinese shrimp Fenneropenaeus chinensis Osbeck

The effects of various salinity fluctuation amplitudes (2, 4, 6 and 8) on the growth, osmolarity, Na⁺-K⁺-ATPase activity and Hsp70 of juvenile Fenneropenaeus chinensis cultured in seawater with a salinity of 20 were studied. The results show that weight gain in the salinity fluctuation treatments was better than that in control; in particular, the weight gain of treatments S4 and S0, at 231.8% and 196.3%, respectively, was significantly different (P<0.05). The hemolymph osmolarity of treatments S0, S2, S4, S6 and S8 was 635.4, 630.8, 623.6, 614.4 and 600.3 mOsm/kg, respectively, and decreased with increasing salinity fluctuation amplitude. The level of Na⁺-K⁺-ATPase activity in gills of F. chinensis was higher than that in hepatopancreas, but there were no significant differences among all treatments, either in gills or hepatopancreas (P>0.05). The relative level of Hsp70 in treatment S4 was 48.4% and 40.4% higher than control in muscle and eyestalks, respectively, with the highest values being recorded under a salinity fluctuation amplitude of 4.     

Effect of Dietary Phosphorus on the Growth and Body Components of Juvenile Synechogobius hasta

The effect of dietary phosphorus on the growth and body components of juvenile Synechogobius hasta was determined. Different percentages of dietary phosphorus (0.63, 0.77, 0.93, 1.06, 1.22 and 1.36) were tested by feeding the fish (body weight, 15.81 g ± 0.32 g; 20 individuals each group; 3 groups each percentage) at a surplus of 5%–10% above satiation for 35 d. Dietary phosphorus did not significantly affect the specific growth rate, feed intake, feed conversion ratio and protein efficiency rate. Nitrogen ...     

The Effect of Temperature on Growth and Energy Budget of the Polychaete,Neanthes japonica Izuka

Growth and energy budget of the polychaete, Neanthesjaponica, at various temperatures (17, 20, 23, 26 and 29 ℃) were investigated in this study. The growth, as indicated by final dry weight and specific growth rate (SGR), increased with increasing temperature, with the maximum level at 26℃, and then decreased significantly at 29℃. A similar trend was observed in feeding rate, food conversion efficiency (FCE) and apparent digestive rate (ADR). However, no significant differences were detected in ADR among all the temperature treatments. In the pattern of energy allocation, faeces energy was only a small component of energy budget and had little influence on the proportion of food energy allocated to growth. The metabolic energy accounted for a large portion of energy intake for each temperature treatment. The nitrogen excretion was appreciable with changing temperature. The two expendi-ture terms (respiration energy and excretion energy) in energy budget were the major factors influencing the proportion of food en-ergy allocated to growth. These results revealed that temperature affected the growth of N. japonica mainly by influencing feeding rate and FCE. In addition, regression equations describing the relationship between feeding rate, faecal production, SGR, FCE and temperature were obtained. The optimum temperatures for feeding rate, FCE and SGR were estimated at 25.01 ℃, 24.24℃ and 24.73 ℃, respectively, from the regression equations.     

不同投喂方式对中国明对虾幼体生长发育及抗病力的研究

研究β-1,3/1,6-D-glucan及不同投喂方式对中国明对虾(Fenneropenaeus chinensis)幼体的存活、生长、发育、非特异性免疫及抗副溶血弧菌能力的影响.将β-glucan以0.25%的剂量添加到微黏合饲料中,以不同的投喂方式投喂对虾幼体,即D0(一直投喂基础饲料);D1(一直投喂含0.25%β-葡聚糖饲料);3D1-3D0(3 d投喂含0.25%β-葡聚糖的饲料-3 d投喂基础饲料);1D1-3D0(1 d投喂含0.25%β-葡聚糖的饲料-3 d投喂基础饲料),共4个处理,每个处理随机设置3个重复,对虾幼体养殖于可控温的半开放系统中,每个养殖桶中盛有50 L盐度为30～32消毒海水,养殖密度为100尾/L.实验结果表明,在孵化后14 d,投喂D1饲料组在总存活率,变态指数,体长,特定生长率方面均高于其它各组,虽然有时差异不显著.在免疫指标方面,在9和14 dph分别进行了酚氧化酶(PO)、碱性磷酸酶(ALP)、超氧化物歧化酶(SOD)、酸性磷酸酶(ACP)活力的测定,结果表明,投喂D1饲料组的4个免疫指标均高于其它3组,虽然有时差异不显著.在14 dph的攻毒实验结果表明,对照组的累积死亡率显著高于D1组(P<0.05),而1D1-3D0和3D1-3D0的累积死亡率则与其它各组均不显著.实验结果表明,间隔投喂方式并不适合于对虾幼体,在中国明对虾幼体饲料中使用β-葡聚糖时,建议添加量为0.25%,并采用一直投喂的方式.     

凡纳滨对虾(Litopenaeus vannamei)继饥饿后恢复生长期间生化组成及能量收支的动态变化

采用氧弹式热量计和常规的动物生化成分分析方法,测定凡纳滨对虾在恢复生长过程中生化组成和能量分配模式的动态变化。结果表明,饥饿状态下,虾体干重、脂肪含量和能值下降,水分和灰分含量升高,但蛋白质含量没有明显改变。恢复投喂后,能量特定生长率显著提高,而虾体各生化组成在不同时间内均恢复到对照组水平。饥饿使对虾恢复生长过程特别是其前期的能量分配模式发生极显著变化。对虾的摄食能分配于生长的比例增大,而损失于代谢消耗的比例则相应减少;摄食能的增加和消化吸收率提高则仅发生在恢复生长的初期。可以认为,凡纳滨对虾继饥饿后的补偿生长效应主要出现在恢复生长的前期,并随时间延长逐步减弱,其生理机制最主要是得益于代谢耗能比例的减少。     

Effective fetch and non-linear four-wave interactions during wave growth in slanting fetch conditions

Wave growth in slanting fetch (with wind blowing obliquely off a coast) is investigated with 7 years worth of routine wave measurements in Lake IJssel in The Netherlands and with the SWAN wave model. Two aspects are considered in particular for this case: the validity of the concept of effective fetch and the role of the non-linear four-wave interactions. For slanting and parallel fetch conditions, we found some significant deviations from the effective fetch assumption, leading to 20–35% mismatch in either the peak period T_p or the significant wave height H_m0 respectively. However, the effect of discrepancies between various widely accepted wave growth formulas turned out to be even more important. The wave directions during slanting fetch are significantly ‘steered’ by the coastline, especially in the first kilometre(s) off the coast. The role of the non-linear four-wave interactions is investigated by running the SWAN (version 40.41) wave model with three different quadruplet formulations. Exact quadruplet methods (Xnl) yielded relatively strong wave steering, despite the four-wave interactions being relatively weak. Application of Xnl did not lead to better overall agreement with measurements — improvements for the mean wave period T_m01 were offset by some deterioration for the wave height H_m0.