条斑紫菜酵母双杂交文库的构建及PyMAPK5互作蛋白的筛选

为进一步研究条斑紫菜促分裂原活化激酶家族PyMAPK5的下游互作蛋白,理解其生物学功能,本研究通过酵母双杂交的方法进行其相互作用蛋白的筛选。提取不同温度和失水逆境胁迫下的RNA,利用Invitrogen体系构建条斑紫菜酵母双杂交cDNA文库,其库容为1.44×107CFU,重组率为91.8%。以pGBKT7-PyMAPK5为诱饵蛋白载体,利用共转化方法,从文库中筛选得到26个与PyMAPK5互作的候选蛋白。候选蛋白集中在光系统II相关蛋白、捕光蛋白、微管蛋白、ATP酶、GTP结合蛋白及假设蛋白等。微管蛋白、捕光蛋白、光系统II蛋白一对一验证结果为阳性,表明在酵母体内存在互作。本研究为阐明条斑紫菜PyMAPK5与其互作蛋白的关系及解析PyMAPK5下游作用机制奠定了基础。     

Current-induced scour beneath initially elevated subsea pipelines

When a subsea pipeline is laid on an uneven seabed, certain sections may have an initial elevation with respect to the far-field seabed, e_o, and thus potentially affecting the on-bottom stability of the pipeline. This paper focuses on quantifying the effects of the upstream dimensionless seabed shear stress, θ_∞, and Reynolds number, Re, on: (1) the maximum dimensionless seabed shear stress beneath the pipe, θ_max, to be compared to the critical shear stress in order to determine whether scour would occur and progress towards an equilibrium state; and, (2) the dimensionless equilibrium scour depth beneath the pipe, S_eq/D. Using a 2-D Reynolds averaged Navier-Stokes (RANS) approach along with the k-ω Shear Stress Transport (SST) turbulence model, a parametric study involving 243 computational fluid dynamics (CFD) simulations was conducted. The simulation results were used to develop a closed-form equation for the prediction of θ_max. Subsequently, experimental measurements of S_eq/D have been compiled from published literature, to develop a new closed-form equation for the prediction of S_eq/D with a high correlation to the experimental data. In summary, we present two closed-form equations for the prediction of θ_max and S_eq/D for pipelines with an initial e_o/D, which are applicable for both clear-water and live-bed conditions. The effects of θ_∞ and Re have been included, albeit Re having a small influence as compared to the other parameters.     

Temporal variation of modulated-wave-train geometries and their influence on vertical bending moments of a container ship

A towing experiment was conducted using a modulated wave train to investigate the vertical bending responses of a hydro-structural container ship model. In the experiment, a spatially periodic modulated wave train, as a model of a freak wave in successive high waves mimicking the so-called three sisters, was generated by the recently established higher-order spectral method wave generation (HOSM-WG) method. HOSM-WG enables us to control the location and timing of the maximum crest height in a wave tank. With precise control of the towing carriage, an experiment was conducted in which the timing of the encounters between the ship model and the modulated wave train was accurately determined. The maximum sagging moment (SM) was found to increase in proportion with the encounter wave height. However, because of differences in the relative depth of the fore and aft troughs, the maximum SM is highly variable for a given wave height. The temporal wave-geometry evolution caused the relative trough-depth to vary significantly within a wave period in the vicinity of the maximum crest height. As a result, depending on the encounter timing, the SM varied considerably for a given wave height. The temporal variation of the wave geometry is a robust feature of a modulated wave train and is common between the spatially periodic and temporally periodic modulated wave trains.     

A stochastic method for the prediction of icebreaker bow extreme stresses

It is well established that the ship-ice interaction process is quite complex and associated ice loads on the icebreaker hull is a stochastic process. Obviously, novel accurate statistical methods and models should be developed and applied to estimate extreme bow stresses.This paper studies icebreaker bow stresses based on measured distribution of ice thickness in the Arctic Ocean on the way to and from the North Pole. Since the vessel route was carefully selected searching for easier ice conditions, the Arctic Ocean crossing was not a straight linear but a meandering path. Thus, the specific ship route data was biased with respect to general ice statistics in the region, but true with respect to the route specific ice data encountered by a ship navigating in that region. Therefore the route specific ice thickness data is directly needed for ship design and navigation analysis. It is assumed that captains are competent and knowledgeable, and therefore will select a route that provides the most favourable ice conditions.This paper contributes to study of the newest Chinese self-designed polar icebreaker, serving the purpose of enhancing icebreaker operational reliability. Finite Element Method software package ANSYS/LS-DYNA has been employed to simulate bow stress pattern for a particular icebreaker operating in the Arctic Ocean. Extreme bow stresses were estimated using Naess-Gaidai method. The latter is a first application of Naess-Gaidai method to a distribution with lower bound. Thus this paper aims at introducing an efficient method of estimating route-specific icebreaker extreme bow stresses.     

Obtaining the distribution of quiescent periods directly from the power spectral densities of Sea waves

There is a growing practical interest in the ability to increase the sea states at which marine operations can be safely undertaken by exploiting the quiescent periods that are well known to exist under a wide range of sea conditions. While the actual prediction of quiescent periods at sea for the control of operations is a deterministic process, the long term planning of future maritime tasks that rely on these quiescent periods is a statistical process involving the anticipated quiescence properties of the forecasted sea conditions in the geographical region of interest. It is in principle possible to obtain such data in tabular form either large scale simulation or from field data. However, such simulations are computationally intensive and libraries of appropriate field data are not common. Thus, it is clearly attractive to develop techniques that exploit standard wave spectral models for describing the quiescence statistics directly from such spectra. The present study focuses upon such techniques and is a first step towards the production of a computationally low-cost quiescence prediction tool and compares its efficacy against simulations. Two significant properties emerge for a large class of wave spectral models that encompasses the ubiquitous Neumann and Pierson Moskowitz or Bretschneider forms. Firstly, the auto-correlation function of the wave profile that are required to produce the quiescence property can be obtained analytically in terms of standard special functions. This considerably reduces the computational cost making desktop computer-based planning tools a reality. Secondly, for each class of these parametric spectra, the probability of a given number of consecutive wave heights (normalised to the significant wave heights) less than some critical value is in fact independent of absolute wave height. Thus, for a broad class of practically interesting wave spectra all that is required to obtain the statistical distribution of the quiescent periods is simple rescaling.     

Semi-analytical solution for the water wave diffraction by arrays of truncated circular cylinders in front of a vertical wall

The present study investigates the combined wave field that is induced by the continuous interaction of plane waves with an array of truncated circular cylinders in front of a rigid wall. The long-term goal of the study is the investigation of possible increase in the efficiency of cylindrical Wave Energy Converters (WECs) by putting in the vicinity of the array a barrier to propagation, a wall, that could assist the reflection of the incoming waves. The main task is to develop a generic solution method that is free of conceptual simplifications employed, e.g. by the method of images and the assumption of “pure” wave reflection. To cope with the set task, the proposed method relies on the semi-analytical formulation of the velocity potentials, while the solution is sought by combined expressions that involve polar and elliptical harmonics. The wall is represented as an elliptical cylinder with zero semi-minor axis. This assumption has eventually a beneficial effect to the underlying formulation given that it simplifies significantly the expansions of the involved diffraction potentials.     

涌浪对风浪能量影响的实验研究

在实验室风浪水槽中进行纯风浪和混合浪波面位移观测,研究波长较长的规则波对风浪能量的影响.本文用混合浪和纯风浪中的风浪显著波的零阶谱矩之比代表混合浪中的风浪与纯风浪能量之比,并以此表征涌浪对风浪能量的影响.研究了该能量比随涌浪波陡S、风区x、波龄倒数u/C、涌浪频率与纯风浪谱峰频率之比fs/fwp的变化规律.结果表明,涌浪对风浪能量的抑制作用随涌浪波陡的增加、波龄倒数的增大及涌浪频率与纯风浪谱峰频率之比的增大而增强.发现该能量比依赖于无因次量R=(1+80(πS)2)1.9(fs/fwp)0.9(u/C)0.27,并拟合得到2者的经验关系.此外,本文实验还发现,在某些情况下,涌浪的存在使风浪能量增加.     

Seasonality and duration in extreme value distributions of significant wave height

This paper presents a statistical model to characterize the long-term extreme value distribution of significant wave height, conditioning to the duration of the storm and accounting for seasonality. A time-dependent version of the peak over threshold (POT) approach is used to build the model, which is then applied to specific reanalysis time series and NOAA buoy records. The model considers the annual and semiannual cycles which are parameterized in terms of harmonic functions. The inclusion of seasonal variabilities substantially reduces the residuals of the fitted model. The information obtained in this study can be useful to design maritime works, because (a) the model improves the understanding of the variability of extreme wave climate along a year and (b) the model accounts for the duration of the storm, which is a key parameter in several formulations for rubble mound breakwater design.     

Onshore sandbar migration at Tairua Beach (New Zealand): Numerical simulations and field measurements

We observed the onshore migration (3.5 m/day) of a nearshore sandbar at Tairua Beach, New Zealand during 4 days of low-energy wave conditions. The morphological observations, together with concurrent measurements of waves and suspended sediment concentrations, were used to test a coupled, wave-averaged, cross-shore model. Because of the coarse bed material and the relatively low-energy conditions, the contribution of the suspended transport to the total transport was predicted and observed to be negligible. The model predicted the bar to move onshore because of the feedback between near-bed wave skewness, bedload, and the sandbar under weakly to non-breaking conditions at high tide. The predicted bathymetric evolution contrasts, however, with the observations that the bar migrated onshore predominantly at low tide. Also, the model flattened the bar, while in the observations the sandbar retained its steep landward-facing flank. A comparison between available observations and numerical simulations suggests that onshore propagating surf zone bores in very shallow water (< 0.25 m) may have been responsible for most of the observed bar behaviour. These processes are missing from the applied model and, given that the observed conditions can be considered typical of very shallow sandbars, highlight a priority for further field study and model development. The possibility that the excess water transported by the bores across the bar was channelled alongshore to near-by rip-channels further implies that traditional cross-shore measures to judge the applicability of a cross-shore morphodynamic model may be misleading.     

Solution of the propagation of the waves in open channels by the transfer matrix method

Many problems in mechanics can be solved by the use of the transfer matrix method. The use of this method in hydraulics engineering is not widespread and only limited studies are available. In this study, linearized St. Venant equations were used and the use of transfer matrix in ocean engineering was investigated for long waves in open channels, and numerical application was carried out. The results obtained through the transfer matrix method, which is quite easy to use, program and comprehend, showed similar results obtained from the characteristics method and finite differences method.