Experimental research on mechanical characteristics and strength criterion of rock of triaxial unloading tests under different stress pathsEI北大核心CSCD

By carrying out triaxial unloading test under different stress paths on rock, the complete stress-strain curves, deformation characteristics and its strength criterion are studied. The test results show that the relation between lateral strain and confining pressure firstly is linear, and then is nonlinear in the total stage of unloading confining pressure. And its growth rate is about 3 to 5 times the growth rate of the axial strain. It shows obvious lateral dilatancy, and the degree of dilatancy is related to the unloading path. According to the area that is enclosed by confining pressure and volumetric strain curve, it can be seen that the greater the confining pressure is before unloading, the more the energy releases. Deformation modulus decreases gradually with the unloading confining pressure, and increases with the initial confining pressure; the relation between deformation modulus and confining pressure shows negative exponential distribution overall. At the same unloading paths, the reduction of the deformation modulus increases with the increase of initial confining pressure. The Poisson's ratio rises up continually, and the variation law with the confining pressure can be well described by nonlinear. Failure characteristics of rock are mainly shear failure. The strength characteristics of rock can be well characterized by using the Mohr strength criterion of power function. These conclusions provide reliable theoretical reference and guiding significance for the deep mining of the underground metal mine.     

脊尾白虾蜕皮抑制激素基因全长cDNA的克隆及其对环境胁迫的响应

为研究脊尾白虾蜕皮抑制激素基因(MIH)在环境适应中的作用,本文采用RACE技术从蜕皮间期脊尾白虾眼柄中克隆获得MIH-like基因全长c DNA序列,命名为Ec MIH。该基因全长1218bp,包括360bp的开放阅读框,420bp的5'端非编码区和394bp的3'端非编码区;开放阅读框编码120个氨基酸,包括41个氨基酸组成的信号肽和79个氨基酸组成的成熟肽,预测蛋白分子量为13.71k Da,理论等电点p I为8.02。同源性比较分析发现,脊尾白虾MIH-like基因与日本沼虾和罗氏沼虾同源性最高,分别为87%和86%。荧光定量PCR分析结果表明,脊尾白虾MIH-like基因在眼柄中的表达量最高,在卵巢中的表达量最少,而在肝脏、肌肉、鳃和血细胞中不表达。环境胁迫后该基因具有明显的时序性。p H6.5胁迫后24h和48h,眼柄和腹神经节中MIH-like基因的表达量较对照组均显著增加(P0.05);4mg/L氨氮浓度组胁迫后12h和24h,眼柄和腹神经节中MIH-like基因的表达量较对照组均显著增加(P0.05);盐度39胁迫后12h和24h,眼柄和腹神经节中MIH-like基因的表达量较对照组均显著增加(P0.05)。本研究结果表明脊尾白虾mih基因参与了环境胁迫后的机体应激反应。     

水杨酸对蛋白核小球藻(Chlorella pyrenoidosa) 生长及抗逆相关基因的影响

以蛋白核小球藻(Chlorella pyrenoidosa)为研究对象, 首先克隆了热激蛋白70(HSP70)和磷 酸甘油酸激酶(PGK)基因全长序列, 然后研究了0-20μg/mL 水杨酸对高温胁迫下HSP70 和PGK 基 因表达, 以及蛋白核小球藻生长、可溶性糖和蛋白含量的影响。结果获得了蛋白核小球藻HSP70 基 因的cDNA 序列2405 bp, 包括60 bp 的5'-非编码区(UTR)、1959 bp 的开放阅读框(ORF)和386 bp 的3'-UTR 和PGK 基因的cDNA 序列1664 bp, 包括35 bp 的5'-UTR, 1398 bp ORF 和231 bp 3'-UTR. 序列比较和分析表明该蛋白核小球藻HSP70 序列与其它绿藻的同源性高达83%-91%, PGK 为 70%-75%.荧光定量PCR 结果显示随着水杨酸浓度的增加, HSP70 和PGK 的表达量在12h 和24h 均有所增加, 而在10 μg/mL 水杨酸浓度下表达量最大, 12h 时分别为对照组的2.57 倍和1.56 倍, 24h 则为1.71 倍和1.79 倍。1-20μg/mL 水杨酸可促进该藻的比生长速率、可溶性糖和蛋白含量的升高。 本文结果表明一定浓度水杨酸对高温胁迫蛋白核小球藻有缓解作用, 且5 和10 μg/mL 水杨酸的效果 最显著。     

九龙江河-海系统夏季浮游植物磷胁迫研究

采用群落水平和细胞水平碱性磷酸酶活性(APA)相结合的方法,研究了2010年夏季(6-7月)九龙江河-海系统(河流-河口-邻近海域)浮游植物的磷胁迫,结果表明,单位叶绿素a的APA在河流下游区[(48.80±14.69)nmol/(h.μg Chl a)]显著高于河口区[(10.60±15.58)nmol/(h·μg Chl.a)]和邻近海域[(23.54±25.41)nmol/(h·μg Chl.a)],表明河流区的浮游植物磷胁迫显著高于河口区和邻近海域.三个区域高比例的游离态APA表明该水域浮游植物已较长时间处于磷胁迫状态.细胞水平的浮游植物荧光标记结果显示,河口区(20％±20％)与邻近海域(38％±20％)无显著差异(P＞0.05),此结果与群落水平APA结果相一致;但河口-邻近海域浮游植物的标记比例显著高于河流下游区(9％±3％)(P＜0.05).     

Shear stress partitioning in large patches of roughness in the atmospheric inertial sublayer

Drag partition measurements were made in the atmospheric inertial sublayer for six roughness configurations made up of solid elements in staggered arrays of different roughness densities. The roughness was in the form of a patch within a large open area and in the shape of an equilateral triangle with 60 m long sides. Measurements were obtained of the total shear stress (τ) acting on the surfaces, the surface shear stress on the ground between the elements (τ_S) and the drag force on the elements for each roughness array. The measurements indicated that τ_S quickly reduced near the leading edge of the roughness compared with τ, and a τ_S minimum occurs at a normalized distance (x/h, where h is element height) of (downwind of the roughness leading edge is negative), then recovers to a relatively stable value. The location of the minimum appears to scale with element height and not roughness density. The force on the elements decreases exponentially with normalized downwind distance and this rate of change scales with the roughness density, with the rate of change increasing as roughness density increases. Average τ_S : τ values for the six roughness surfaces scale predictably as a function of roughness density and in accordance with a shear stress partitioning model. The shear stress partitioning model performed very well in predicting the amount of surface shear stress, given knowledge of the stated input parameters for these patches of roughness. As the shear stress partitioning relationship within the roughness appears to come into equilibrium faster for smaller roughness element sizes it would also appear the shear stress partitioning model can be applied with confidence for smaller patches of smaller roughness elements than those used in this experiment.     

Expression of heat shock protein 70 in a permanent cell line (EPC) exposed to sediment extracts from the North Sea and the Baltic Sea

Heat shock proteins 70 (hsp70) are known to be induced by a great variety of chemical stressors. The effects of different environmental contaminants, which were identified in sediments of the North Sea and the Baltic Sea as main contaminants or as contaminants of special toxicological interest, on the extent of expression of hsp70 in a permanent cell line Epithelioma papulosum cyprini (EPC) were investigated. Concentrations of the single contaminants leading to elevated hsp70 levels were compared to the respective concentrations of these contaminants encountered in the sediment extracts thus allowing an assessment of their contribution to the toxicity of these sediments. Analysis of hsp70 was performed using western blotting techniques with subsequent comparative quantification by densitometry. Eleven of thirteen contaminants have shown a dose-dependent increase in hsp70 levels. An expression of hsp70 was observed in most cases between 0.1 and 10 microg/mL test solution. Some of the investigated stressors led to extremely high hsp70 contents even at low concentrations. However, in most cases contaminant concentrations in sediments were too low to cause an effect. For this reason, effects of the tested sediments are attributed to other contaminants or rather to mixtures of stressors.     

大断面小净距大帽山隧道现场监控量测及分析

结合大帽山隧道的工程实践,通过围岩内部位移、拱顶沉降、围岩压力和锚杆应力的现场监控量测工作,研究复杂地质条件下大断面小净距隧道双侧壁导坑法施工时围岩的稳定性。阐明分导洞开挖时围岩内部位移的变化趋势、特点及位移场,相邻导洞施工时的相互影响,围岩与支护结构间的相互调整变形机制,拱顶沉降捕捉的变形小于围岩实际变形的原因,支护结构的压力和锚杆应力状态及其与围岩位移的变化关系。监测结果表明,大断面小净距隧道Ⅴ级围岩段的破碎带采用现有的施工工艺和支护参数是可行的,围岩变形可控,支护结构的支护效果显著,围岩基本稳定。研究的方法、分析和结论可为类似条件下隧道工程的设计、施工、监测和进一步的理论研究提供参考和借鉴。     

黄河入海泥沙沉积固结过程抗侵蚀性变化研究

河口入海泥沙沉积固结过程中抗侵蚀性的变化直接决定着沉积物的再悬浮和二次迁移,对河口岸滩的稳定具有重要决定作用。在现代黄河三角洲潮滩模拟入海泥沙快速沉积,现场测试不同固结时间沉积物的抗侵蚀性和物理力学指标的变化。研究发现,黄河入海泥沙沉积物的抗侵蚀性随固结时间增长迅速提高,当沉积固结时间达8h时,其临界侵蚀切应力就超过了原状潮滩表层沉积物;新沉积泥沙的临界侵蚀切应力与其重度、贯入阻力、剪切强度呈良好的正相关关系,与含水率呈良好的负相关关系。黄河入海泥沙临界侵蚀流速的试验值随固结时间的增长速率要高于各泥沙起动公式计算值的增长速率,前者是后者的1.5～4.1倍。     

软土隧道盾构出洞灾害的渗流应力耦合分析

对盾构出洞口涌水涌砂灾害问题作了分析。利用Midas-GTS软件,建立了盾构出洞施工的有限元模型。考虑盾构出洞施工的渗流-应力耦合效应,研究了盾构工作井外加固土体前方高水位工况下,地下水绕加固土体下卧土层的渗流规律以及加固土体前方原状土的沉降规律。研究结果表明,在假定井外加固土体完全隔水情况下,洞门涌水涌砂灾害的发生与否受到土体加固长度、井外地下水等因素影响;土体加固长度的增加对工程安全有利,但成本较高;地下水位的降低能有效减小盾构出洞洞门涌水灾害的发生,需综合考虑工程安全性、经济性和工期等来确定合理的施工参数。