黑鲷(Acanthopagrus schlegeli)肿瘤坏死因子α cDNA的克隆及特征分析

采用同源克隆的方法，从黑鲷中获得肿瘤坏死因子(TNFα)全长cDNA序列。该序列含1288个核苷酸，可编码253个氨基酸的TNF前体蛋白。它是一种跨膜蛋白，无糖基化位点和信号肽结构。黑鲷TNFα与其它鱼类的TNFα的相似性很高，占据进化上独立的分支；与哺乳类TNFα和TNFG源于共同的祖先。基因结构分析显示，该基因含有TNF家族pmfile和TNF家族的标签序列；在参与TNFα基因二硫键形成的两个半胱氨酸和TNFα三聚体形成的位点高度保守；空间结构模拟显示，它与哺乳类TNFα的空间结构相似，都是由两个β折叠片组成，每个折叠片包含5个反向平行的β链。表达研究结果表明，黑鲷TNFα在检测的各个组织中均为组成型表达，表现为在刺激与非刺激鱼体中，都可以检测到黑鲷TNFα的表达，但是其表达水平在不同组织中有很大差异。黑鲷TNFα在头肾、脾脏和鳃的表达量较高，而在心脏、肝脏、血液、肾脏和大脑中表达量较低。     

思林水电站通航建筑物高边坡稳定分析和评价

应用ANSYS结构分析软件,模拟了思林水电站通航建筑物高边坡的地形、地质条件以及开挖的过程,建立边坡开挖的有限元计算分析模型,分析边坡在自然状态、分级开挖以及锚杆支护加固等工况下的应力、变形及其整体稳定性.定义单元的局部安全系数,得到了边坡开挖加固后稳定安全系数的分布.根据计算成果,指出现设计加固措施可以保证边坡稳定,无需增加其他工程措施.     

电磁波信号在对流层中路径弯曲改正的级数展开式

大气折射延迟是空间大地测量中的一个主要误差源。本文严格地给出了中性大气路径弯曲改正的原理性公式。通过对各种因素量级的分析,在亚毫米量级的精度上给出了一个以视天顶距正切和正割乘积为参数的级数展开模型。新模型在球对称大气模型下,级数展开模型的精度达到了毫米量级,理论上可以展开到任意阶次,且可允许观测高度角小到5°。     

数字水准仪标尺编码原理研究

数字水准仪的标尺编码规则直接影响其测量精度.基于现有几种数字水准仪标尺编码的特点,归纳出标尺条码的编码衡量指标:信息密度、分辨率和纠错能力.以条码的最大编码容量与单个码区长度之比值衡量其承载的信息密度;利用光学系统的点扩散函数研究条码图像的分辨率,即相邻边缘的相互影响,得出码元相邻边缘的间距相等时,影响最小的结论;用相关系数或编码所用函数本身的性质表征条码的纠错能力.依据编码指标建立一套新的标尺编码规则:以格雷码为数值码,固定宽度的码元为参考码,两者交替组合.分析表明,该种编码不但信息密度大,分辨率高,而且纠错能力强.     

对提高导航型GPS定位精度之工作方法的探讨

论文主要介绍了导航型GPS坐标转换参数的计算方法和测量误差的改正方法。该方法的要点是通过实测多个公共点来计算坐标转换参数,以此获得较好的转换精度;并进一步对观测结果加以误差改正,从而提高其定位精度。     

基于BP神经网络的地图数字化误差纠正

由于在数字化采集过程中不可避免地会引入系统误差和异常误差,因此消除和削弱这些误差的影响是提高空间数据质量的关键。然而由于图纸变形不均匀,扫描误差又极其复杂,用常规的多项式拟合技术只能消除部分有规律的系统误差,很难完全消除它们对地图数字化坐标的影响。BP神经网络是一个高度非线性映射系统,能以任意精度逼近。结合地图数字化坐标改正的特点,本文给出了基于BP神经网络地图数字化坐标误差纠正的方法,并通过实例验证了该方法的有效性。     

GALILEO局域增强完备性监测网布设研究

完备性是飞机飞行安全的重要指标之一。我国在各种网的布设中一般只注重精度,而忽略了完备性。本文利用GALILEO模拟数据,在飞行轨迹固定的情况下,通过增减监测站的数量以及变换监测网的形状,计算飞机的精度和垂直保护水平(VPL),并分析飞机在不同监测网中精度和垂直保护水平的的变化规律,可以知道飞机在正方形网形的整体精度好于在其他网形中的精度,飞机在在正方形网形中的VPL虽大于在五边网形中的VPL,但两者相差很少。综合考虑,正方形网形是局域完备性监测网的最优布设网形。     

全站仪交汇法在高速公路路面沉降监测中的应用

文章介绍了全站仪交会法沉降监测,方便简洁地解决了测量人员不能进入的高速公路等特殊位置的测量问题,并通过对该方法的精度分析,论证了其可行性和有效性,对以后类似的测量工作提供经验和依据。     

一种改进的周跳多项式拟合方法

多项式拟合探测周跳受相位观测值所含误差的影响,随着采样数的增加,其探测周跳的能力随之降低,通常将观测数据分成若干段来进行处理。这样不可避免的增加工作强度,降低工作效率。本文根据多项式拟合探测周跳的基本原理,对多项式进行改进。经实验证明,利用改进后的多项式进行拟合能够有效地弥补传统方法的不足,使周跳的探测与修复更加稳定可靠。     

Main Controlling Factors on Hydrocarbon Accumulation and Distribution in Marine Sedimentary Sequences in South China

Multiple source rock assemblages were deposited in the sedimentary provinces in South China in geologic history,and some of them were destructed by and some survived against multiple tectonic movements.Therefore,multiple sources,mixed sources,and uneven distribution of sources occurred in the marine sedimentary basins in South China during the late stage of hydrocarbon pooling.Epidiagenesis of the marine carbonate reservoirs and its modification to reservoir poroperm characteristics determined the formation and the scale of natural gas pools.The exploration practices show that the large to medium gas fields mainly occur in areas with high-quality reservoirs.Detailed study of the paleo-oil accumulations and typical oil and gas reservoirs reveals that the basins experienced multiphase superimposition and modification,leading to the distribution of the Paleozoic paleo-oil accumulations and bitumen in the peripheral areas.The phenomenon that oil and gas production concentrates in the Sichuan basin indicates that the overall sealing conditions of a basin determine the oil/gas potentials and the scale of oil and gas production.This is a critical factor controlling the accumulation and distribution of gas in the marine sequences in South China.The early oil and gas pools in the Yangtze platform left billions of bitumen in the peripheral areas due to the destruction of seals.Since the Himalayan,"late-generation and late-accumulation" gas pools represented by the gas pools in the Sichuan (四川) basin were formed in the marine sedimentary sequences in South China as a result of the change of the sealing conditions.Current gas discoveries appear to be "paleo-generation and paleo-accumulation" gas pools but actually are "late-generation and late-accumulation" gas pools.These patterns of hydrocarbon pooling clearly depict themselves in western Sichuan basin and Weiyuan (威远)gas field.It is revealed that the gas pools in the Sichuan basin were mainly formed as a result of hydrocarbon phase change (thermal cracking of oil to gas),miscible migration,and dynamic equilibration since the Himalayan.A large number of gas pools were formed in the Himalayan and the gas pools in the marine sequences are characterized by late pooling; this kind of gas fields/pools are controlled by:(1) effectiveness of modification and superimposition of the marine basins,(2) effectiveness of the source rocks,(3) effectiveness of the overall preservation conditions,and (4) effectiveness of plays.