Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

Initiation of primary cell culture from amphioxus <Emphasis Type="Italic">Branchiostoma belcheri tsingtauense</Emphasis>

Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C. Supported by Doctoral Initial Fund of Ludong University (No.43304)     

The impact of physical processes on pollutant transport in Hangzhou Bay

A Lagrangian tracer model is set up for Hangzhou Bay based on Coupled Hydrodynamical Ecological model for Regional Shelf Sea (COHERENS). The study area is divided into eight subdomains to identify the dominant physical processes, and the studied periods are March (the dry season) and July (the wet season). The model performance has been first verified by sea-surface elevation and tidal current observations at several stations. Eight tracer experiments are designed and Lagrangian particle tracking is simulated to examine the impact of physical processes (tide, wind and river runoff) on the transport of passive tracer released within the surface layer. Numerical simulations and analysis indicate that: (1) wind does not change the tracer distribution after 30 days except for those released from the south area of the bay during the wet season; (2) the tide and the Qiantang River runoff are important for particle transport in the head area of the bay; (3) the Changjiang River runoff affects the tracer transport at the mouth of the bay, and its impact is smaller in the dry season than in the wet season. Supported by the Natural Science Foundation of China (No.40576080); National High Technology Research and Development Program of China (863 Program, No. 2007AA12Z182)     

实时操作系统集成调度的两级方案设计

实时操作系统的关键技术在于系统如何调度应用程序。由于多类型的实时任务并存于一个系统，单一的调度算法无法满足这一需要，于是提出了集成调度的两级方案。介绍了这一方案的设计思想及可行性，并根据这一方案，提出了一个设计框架的设想。     

基于GNSS的单波束测深系统在大中型水库水下地形测量中的应用

随着水库在经济社会发展中所起的作用日趋明显,对水库的开发、治理更加重视,获取高精度的水库水下地形数据变得更加急迫.随着测深技术、差分GNSS技术以及姿态传感器的不断发展,基于GNSS的测深系统为水下地形测量提供了全新的解决方案.通过合理规划测线,经过声速改正、吃水改正,利用船载GNSS导航定位和测深仪采集测深数据,经转...     

A study on the reference frame consistency in recent Earth gravitational models

All gravity field functionals obtained from an Earth gravitational model (EGM) depend on the underlying terrestrial reference frame (TRF), with respect to which the EGM’s spherical harmonic coefficients refer to. In order to maintain a coherent framework for the comparison of current and future EGMs, it is thus important to investigate the consistency of their inherent TRFs, especially when their use is intended for high precision studies. Following the methodology described in an earlier paper by Kleusberg (1980), the similarity transformation parameters between the associated reference frames for several EGMs (including the most recent CHAMP/GRACE models at the time of writing this paper) are estimated in the present study. Specifically, the differences between the spherical harmonic coefficients for various pairs of EGMs are parameterized through a 3D-similarity spatial transformation model that relates their underlying TRFs. From the least-squares adjustment of such a parametric model, the origin, orientation and scale stability between the EGMs’ reference frames can be identified by estimating their corresponding translation, rotation and scale factor parameters. Various aspects of the estimation procedure and its results are highlighted in the paper, including data weighting schemes, the sensitivity of the results with respect to the selected harmonic spectral band, the correlation structure and precision level of the estimated transformation parameters, the effect of the estimated differences of the EGMs’ reference frames on their height anomaly signal, and the overall feasibility of Kleusberg’s formulae for the assessment of TRF inconsistencies among global geopotential models.     

GISVlog模型的设计与实现

本文以地理信息系统(GIS为基础,分析了地理信息系统沃客(GISVlog,)形成的两个背景——网络新技术提高GIS生命力和沃客(Vlog,Vedio log的缩写)为网络增值;分别论述了GISVlog形成对GIS发展和Vlog发展的意义;在普通GIS的系统功能结构和Vlog的系统功能结构的基础上构建GISVlog的框架结构并加以实现;论文最后展望了GISVlog的发展前景,提出GISVlog是网络搜索技术新的研究方向。     

局部角度域波传播步进算法研究

本文从非均匀介质中波动方程出发,提出了基于一般标架的相空间（局部角度域）波传播的步进算法. 该方法在构造单程波的步进算法时,在选择标架或正交基等方面有更大的自由度. 我们以不随频率及深度变化且具有变尺度特性的Gabor_Daubechies紧标架为例,给出了单程波传输算子的具体形式及相应的波场步进算法;详细讨论了基于Gabor_Daubechies标架的传输算子的高频渐近展开问题,得出了在高频、小传输步长条件下传输算子的近似解析表达式,并给出使用条件. 通过模型算例,比较了精确传输算子与高频近似传输算子用于非均匀介质中波传播的结果,说明在一定条件下由两者得出的波场几乎是相同的.     

共炮检距道集波动方程保幅叠前深度偏移方法

本文提出了一种基于双平方根算子的共炮检距道集波动方程保幅叠前深度偏移方法,将振幅误差补偿作为偏移的一部分与“运动学偏移”一起在偏移过程中实现.其基本内容包括：(1)从保幅的单平方根算子方程出发,推导出由双平方根算子定义的保幅单程波方程;(2)根据地震波摄动理论把速度场分裂为层内常速背景和变速扰动,分别在频率－波数域和频率－空间域求得波场深度延拓的偏移时移量及振幅校正系数,从而得到最终的DSR保幅波场延拓算子;(3)在高频假设条件下,把DSR保幅波场延拓公式中的积分运算进行稳相近似,得到保幅波场延拓的相移公式.理论分析和模型数值试验表明,该方法不但可以使散射能量聚焦、归位,提高成像精度;而且可以输出正确反映地下反射系数的振幅信息,为后续的地震属性分析（如AVO/AVA）提供更真实的地震信息.     

新疆生态系统健康遥感评估及关键驱动因子研究

作为我国陆地面积最大的省级行政区,新疆维吾尔自治区具有多样的生态系统结构和丰富的动植物物种资源,是我国西北地区重要的生物多样性热点研究区域.通过以新疆维吾尔自治区为研究对象,结合区域特点和定量遥感数据实时、易获取、周期性等优势,选取归一化植被指数、动物物种丰富度、干旱度、人类扰动指数四类单因子指标,依据"活力-组织-恢...