Assessing multiple mating and reproductive skew in three species of clinid fishes (Blenniiformes: Clinidae: Clinini), in reference to multiple mating in viviparous teleosts

This study investigated multiple mating in three species of intertidal klipfishes (family Clinidae): super klipfish Clinus superciliosus, bluntnose klipfish Clinus cottoides, and nosestripe klipfish Muraenoclinus dorsalis. These species display the rare reproductive mode of female vivipary with superfetation, a phenomenon where offspring at various stages of development are found within the female. Three or four microsatellite loci were used to genotype 25–30 adults of each species, as well as embryos of 131 C. superciliosus from six broods, 103 C. cottoides from six broods, and 63 M. dorsalis from five broods. The presence of multiple mating by females was assessed by maximum-likelihood sibship reconstruction implemented in the program COLONY, and the non-random distribution of reproductive success among individuals (reproductive skew) within broods was tested using the binomial skew index. Multiple mating by females was pervasive and observed in 83%, 100% and 80% of the broods of C. superciliosus, C. cottoides and M. dorsalis, respectively. The mean number of sires per brood was 4.3, 3.0 and 3.2, and, correspondingly, 5/5, 5/6 and 1/4 of multiply-sired broods showed significant reproductive skew in C. superciliosus, C. cottoides and M. dorsalis, respectively. The presence of pronounced reproductive skew may indicate post-copulatory processes taking place that bias paternity. We compared our results on multiple mating within the klipfish family, and with four other fish families that have vivipary with superfetation, and found the klipfish patterns were among those of live-bearing vertebrate groups with the highest average numbers of sires per brood.     

精密单点定位的一种低动态解法

采用自适应选权滤波法,对低动态精密单点定位的解算方法进行研究,并编写了精密单点定位程序。通过合理选择自适应因子参数,对船载动态GPS测量数据进行了事后精密单点定位处理。结果表明:相对于经典卡尔曼滤波方法,采用自适应选权滤波法可大大提高低动态精密单点定位解的精度,X、Y、Z三个方向的RMS分别提高了79.9%、69.7%和92.0%。     

The sensitivity of long-term yield targets to changes in fishery age-selectivity

Maximum sustainable yield (MSY) has been adopted as the primary management goal by several inter-government fishery organisations, and in the United States it forms the cornerstone of federal fishery management policy. MSY became a strategic goal for the management of Europe's fisheries following the resolution of the World Summit on Sustainable Development in 2002 to restore or maintain fish stocks to MSY levels by 2015. Calculation of MSY requires information on the rate at which biomass increases through growth and reproduction and the rate at which it decreases through natural mortality and fishing. Population-selection, which measures the age-specific rates of fishing mortality, is a key component for the calculation of yield as a function of fishing mortality and MSY, yet selection rarely features in either management advice or sensitivity analyses. Effective management of selection can potentially lead to increases in long-term yield, but before taking action managers need to understand what long-term increases are possible. Using a hypothetical stock, equilibrium yield curves were calculated for three scenarios in which the shape of the population-selection curve varied. The results illustrate the potential extent of variation in MSY and the corresponding fishing mortality required to achieve it (F_MSY) that may result solely due to changes in population selectivity. They show that relatively subtle changes in selection can produce substantial differences in MSY and F_MSY. The results are discussed with specific reference to the development of long-term management targets and the mechanisms by which managers might try to influence population-selection.     

A System for Fast Cartographic Sounding Selection

Soundings on nautical charts provide information about the shape of the ocean bottom between chart depth curves. A single chart may have thousands of soundings posted on it. This article describes a system for automatic cartographic sounding selection. In this system, a new algorithm for automated selecting soundings is developed. The algorithm is employed in such a way that it can guarantee (a) fast and accurate selecting sounding, (b) processing the circular depth curves, (c) solving the overplotting problems between depth curves and soundings, and (d) keeping selected soundings “prettier.” The system was intensively tested using real data sets, and its superiority has been revealed by the testing results.     

Importance of feeding on regenerable parts of prey for juvenile stone flounder Platichthys bicoloratus in estuarine habitats

Prey availability is one of the most important factors affecting the quality of nursery grounds. Estuaries play an important role as nursery grounds for juvenile stone flounder, but the mechanism behind the consistently high availability of prey has never been examined. This study investigates which prey is mainly selected by juvenile stone flounder (15–55 mm standard length) in the estuary of the Natori River, northern Japan. In a cage experiment, juveniles showed positive selection for the palps of the spionid polychaete Pseudopolydora kempi in March, and for the siphons of the bivalve Nuttallia olivacea in April, May and June in both sandy and muddy-sand habitats. This selective predation showed that sublethal predation on regenerable parts of invertebrates is important for stone flounder. Nuttallia olivacea, the dominant bivalve in the estuary, was more abundant and in better somatic condition in the sandy area in spite of the stronger siphon-cropping pressure by juvenile stone flounder. These results confirm that sublethal predation on highly abundant benthos plays an important role in forming estuarine habitats into areas of high prey availability for juvenile stone flounder, which leads to their high growth rate.     

两种四角蛤蜊(Mactra veneriformis) 壳内色品系选育初探

采用通径分析原理及个体间随机交配的方法,进行了两种壳内色四角蛤蜊的形态性状差异及品系选育研究。结果表明,两种壳内色四角蛤蜊除颜色不同外,其它形态性状间无显著差异(P>0.05);两者的4个数量性状之间的相关系数均达到极显著水平(P<0.01),但影响重量性状的主要形态性状并不相同;壳形态性状对活体重的相关指数均大于0.85,表明所选的壳形态性状是影响活体重的重点性状。两个品系D型及3日龄幼虫大小无显著差异(P>0.05),6、12和15日龄紫色品系的壳长和生长速度均高于白色品系,差异极显著(P<0.01);幼虫期间,两者的存活率无显著性差异(P>0.05),但紫色品系的孵化率和变态率明显高于白色品系(P<0.01)。稚贝室内培育期间,白色品系的壳长明显大于紫色品系(P<0.01);但室外养成期间,两者差异逐渐缩小,90日龄两者壳长已无显著性差异(P>0.05);稚贝期间两者的生长速度及成活率均无显著性差异(P>0.05)。     

日本鼓虾与鲜明鼓虾线粒体基因组全序列的分析比较

首先通过基因组DNA的提取、通用引物PCR扩增和长PCR扩增,从而获得日本鼓虾(Alpheus japonicus)的线粒体DNA;应用鸟枪法和引物步移法测序,获得了日本鼓虾的线粒体基因组全序列。结合GenBank线粒体基因组数据库中鲜明鼓虾(A.distinguendus)的线粒体基因组,比较分析了鼓虾线粒体基因组的基本特征、基因排列、蛋白质编码基因、选择压力和差异位点等。研究结果表明,在日本鼓虾线粒体基因组中共存在9对基因的重叠。日本鼓虾线粒体基因组全长16 487 bp,较鲜明鼓虾线粒体基因组(15 700 bp)长,主要是由于最大非编码区的长度存在差异。日本鼓虾与鲜明鼓虾线粒体基因组均编码37个基因,且37个基因的基因排列完全一致;与泛甲壳动物线粒体基因组的原始排列相比,仅出现1个转运RNA基因(trnE)的易位和倒位。2个鼓虾线粒体基因组蛋白质编码基因的起始密码子和终止密码子存在差异。除了cob基因外,其余12个蛋白质编码基因所编码氨基酸的数目均完全相同。鼓虾线粒体基因组13个线粒体蛋白质编码基因的Ka/Ks比值都远远低于1,显示出较强的负选择。在15个主编码基因中,nad5基因的变异位点数最多,其次是nad4基因和lrRNA基因。因此,nad5,nad4和lrRNA基因可以作为备选的分子标记,用于分析鼓虾不同物种和群体之间的生物多样性。     

中韩海底光缆(西段)弃置方案可行性研究

中韩海底光缆已于2005年7月宣布废弃.中韩海底光缆是我国宣布废弃的第一条国际海底光缆.通过对中韩海底光缆多种可能的弃置方案进行分析,对比研究了各种弃置方案的利弊及可行性.通过比选,提出了全线打捞回收方案是最佳方案;领海以内打捞回收方案次之,是具有现实性和可操作性的方案;原地弃置方案是最不适合中韩光缆弃置的方案.     

多棘海盘车对太平洋牡蛎、紫贻贝、菲律宾蛤仔摄食选择性的研究

以多棘海盘车(Asterias amurensis)为试验对象,研究了多棘海盘车对3种双壳贝类的摄食选择性、摄食过程中用于搜寻和处理饵料生物的时间分配以及摄食机制等。试验以选择指数、捕食速率、日平均摄食率对多棘海盘车的摄食行为及搜寻和处理时间比进行了描述和统计。结果表明:各处理组多棘海盘车对菲律宾蛤仔的选择指数均为最高,占3种饵料生物的70%以上,显著高于太平洋牡蛎和紫贻贝(P<0.05),而各处理组对太平洋牡蛎的选择性均最低。多棘海盘车对菲律宾蛤仔的捕食速率和日平均摄食率显著高于太平洋牡蛎和紫贻贝。研究发现,在各处理组中多棘海盘车用于搜寻饵料生物的时间无显著差异,但随饵料密度的增加,用于处理饵料的时间逐渐减少。