网箱养鱼诱发有机物污染的生物监测

介绍三种通过底栖动物群落结构的变化对有机物污染监测和评价的方法,并分别对澳大利亚塔斯马尼亚州Nubeena鳟鱼养殖场内由高密度网箱养鱼诱发的有机物污染进行了检测。结果表明:距网箱垂线0m处为严重污染区域,10m处为中度污染区域,30m为过渡类型,其有机物对该处底栖生物仍具有害影响,60m处为轻度有机物富集区,但该处有机物对底栖生物的总效应是有益的,60m以外为正常区域。本文还对三种方法的适用性进行了讨论。     

弯睾鲫吸虫[Carassotrema flecterotestis(Zhukov,1971)comb.nov.]及其毛蚴发育观察

本文对取自台湾海峡棱鮻 (Lizacarinatus)消化道的弯睾软穴吸虫 (HapalotremaflecterotestisZhukov, 1971)进行重新描述,并对其毛蚴的体外发育进行观察.根据Yamaguti(1971)区分单孔科 (Haploporidae)和叉盘科 (Waretrematidae)的标准,同意李敏敏(1984)将其从单孔科移出并入叉盘科.在与叉盘科已知属比较后,我们发现该吸虫除睾丸形状外,其他主要特征与鲫吸虫属 (CarassotremaPark, 1938)的相似.因此,我们拟将其作为后者的同物异名,并将该吸虫重新命名为弯睾鲫吸虫 [Caras sotremaflecterotestis(Zhukov, 1971)comb. nov. ].实验结果表明:在室温 7~24℃(均值为 15. 3℃)条件下,其虫卵从单个卵细胞到毛蚴孵出共需 12d.     

Molecular identification of ascaridoid nematodes from the deep-sea onion-eye grenadier (Macrourus berglax) from the East Greenland Sea

Macrourus berglax from the East Greenland Sea was studied for the presence of ascaridoid nematodes in 2001, 2002 and 2003. The fishes were collected between 278 and 413 m water depth using a benthopelagic net. Based on the amplification of the internal transcribed spacer ITS-1, 5.8S, ITS-2 and flanking sequences (=ITS+), three ascaridoid nematode species were identified. The prevalence of infestation during the 3 years ranged from 42.9% to 62.9% and 22.9% to 40.0% for the anisakids Anisakis simplex (s.s.) and Pseudoterranova decipiens (s.s.), respectively, and from 28.6% to 60.0% for the raphidascarid Hysterothylacium aduncum. A total of 18 specimens, two of each species and examination year, revealed no sibling species, suggesting a limited distribution of other ascaridoid siblings into the deep sea. The ITS-1, 5.8S and ITS-2 sequences of A. simplex (s.s.) from the East Greenland Sea did not differ from previously published sequence data (GenBank) from other regions in the Atlantic and Pacific oceans. The sequences of P. decipiens (s.s.) corresponded most closely to those of specimens from Richardson Bay, western Pacific, and differed in four positions (0.5%). They corresponded least to those of specimens from Japan (1.5%). The sequence data for H. aduncum differed in two positions in the ITS-1 (0.2%) and three positions in the ITS-2 (0.3%) from sequences from Japan. A high genetic similarity between the regions can be explained by (a) extensive final host migration in the case of A. simplex (s.s.), (b) an overlapping distribution of final host populations along the continental shelves for P. decipiens (s.s.) and (c) a low host specificity and large population size in the intermediate and final hosts for H. aduncum. The occurrence of the identified species in the macrourid fish underlines the potential of cosmopolitan ascaridoid nematodes to distribute not only horizontally but also vertically in the deep sea.     

山东省大中型水库水化学性状研究──几种主要水化因子的变化及其在评价水库鱼产力中的作用

本文通过对山东省３８座代表性大、中型水库总氮（ＴＮ）、总磷（ＴＰ）、化学耗氧量、硬度及电导率（λ）五种主要水化因子连续两年（１９８９～１９９０）的调查与研究，探讨了水化因子与水库鱼产力的关系。用反映鲢、鳙生长状况的综合生长指数（ＧＩ）作为水库鱼产力的指标。它与水化因子相关分析表明，总磷是山东省３８座水库鱼产力的主要影响因子。提出ＧＩ与总磷、总氮及电导率等水化因子的逐步回归方程关系非常显著。证明利用水化因子作为水库鱼产力的评价指标是可行的。并指出：同一水库的相同水化因子存在年间差异，其主要原因在于降雨量和水库进水量的不同，鉴于此．同一水库鱼产力也存在年间差异。     

Early diagenesis of nurtrients （C, N, P and Si） stored in sediments of two reservoirs in southwestern China

Damming is a common anthropogenic intervention along the course of rivers, which is defined as ＂artificial-lake effect＂, both in China and across the world. Today as many as 48000 dams and/or reservoirs are in operation in the Changjiang River （Yangtze River） drainage area, and more are being constructed. While damming is well known to affect riverbome nutrient loads, and thus the riverine ecosystems owing to removal of carbon fixation, and removal of particles in reservoir sediments, there is limited information on the detailed early diagenesis of sediments in reservoirs including the regeneration processes of nutrients deposited in sediments and exchange flux across the sediment-water interface, which is important for mass balance of riverbome nutrients. In the present study, two large-size reservoirs, Wujiangdu Reservoir （WJDR） and Dongfeng Reservoir （DFR）, located on the main steam of the Wujiang River and with uniformity hydrography and discrepancy biogeochemical activity （e. g. primary production）, were selected for a comparative study on the detailed processes of nutrient regeneration. Water, pore water and sediment were sampled from these two reservoirs and dissolved organic carbon （DOC）, NH4^＋, NO3^-, PO4^3-, dissolved silica （DSi） in the overlying water and pore water, and total organic matter in sediments were determined. The results of correlation analysis suggested that in these two reservoirs, processes of nutrient regeneration near the sediment-water interface were significantly different. As a result of rapid decomposition of algae-derived ＂labile＂ organic matter in upper sediments, nutrient regeneration processes and upward fluxes in WJDR are dramatically stronger than those of DFR. NH4^＋ upward flux from sediment in WJDR was about 17 times higher than that in DFR. PO4^3- flux in WJDR is about 13 times above that in DFR. DOC flux in WJDR is larger that in DFR by 5 times, and DSi by 1 time.     

Quantification of the risk associated with high levels of mercury pollution at Temirtau, Kazakhstan

This research aims to establish the risks of hurnan exposure to modeling approach. The study site was located on the River Nura 1990s, an acetaldehyde production plant located in the industrial high environmental mercury levels, based on a quantitative risk in central Kazakhstan. From the 1950s until its closure in the mid city of Temirtau released mercury-containing wastewater into the river. River sediments are highly contaminated with mercury up to a distance of 25 km and beyond. In addition, a local power station released an estimated 6 million tonnes of fly-ash into the water and the mercury has become quite tightly associated with the ash deposits. River water, fish and agricultural land in the floodplain are also contaminated with mercury, yet the risks posed to the local population have not been evaluated to date. In June and July 2005, we took samples of soil, interior and exterior dust, drinking water, and food from individual households, communal areas and markets. Additionally, water and sediment samples and fish were taken from the river. Interviews were conducted with householders to establish their age and body weight, general living conditions and sources of irrigation and drinking water. A food frequency questionnaire （FFQ） was designed to investigate the frequencies of consumption of several common regional food items, including fish from the fiver and/or local market. Human hair samples were also collected to estimate the mercury bioburden and to enable the validity of the modeling approach to be established. The paper expands on the main pathways of contamination and looks at linkages between exposure pathways and mercury concentrations found in human hair. Uncertainties inherent in risk analysis as well as their influence on the relative importance of different exposure routes are also discussed.     

Verification of the dualism ordering method by the canonical correspondence analysis: fish community samples

Two ordination techniques (Romaniszyn diagram, RD, and Canonical correspondence analysis, CCA) were applied to the same data set (fish assemblage composition along two Paraná river tributaries and associated environmental variables), to identify spatial community patterns and further to evaluate the performance of RD. The major patterns in these Brazilian streams were successfully revealed by both methods (i.e. clear separation of both tributaries on the basis of fish assemblage composition and environmental factors), but more complex situations may limit the application of RD in comparison with CCA.