Advanced characterization of marine dissolved organic matter by combining reversed-phase liquid chromatography and FT-ICR-MS

Marine dissolved organic matter (DOM) was separated by reversed-phase (RP) liquid chromatography method and analyzed with fluorescence/absorption detection and Fourier transform ion cyclotron mass spectrometry (FT-ICR-MS). The three key characteristics of the RP method are: (a) The C18-RP column chosen provides enhanced separation when the aqueous phase is 100% buffer-free water, and it does not degrade over time; (b) the water eluent adjusted to pH 7 significantly improves the resolution of water soluble compounds; (c) the initial flow maintained at low levels improves the separation of polar compounds. In samples, containing “fresh” DOM, specific peaks were detected, which were absent in “old” DOM samples. The combination with size exclusion chromatography (SEC) also demonstrated the relation between polarity and molecular size of DOM. FT-ICR-MS was applied to evaluate the quality of separation on a molecular scale demonstrating that physico-chemical characteristics of DOM can be related to molecular formulas. Sample extracts were separated into 4 preparative fractions, and a large suite of the identified molecular formulas only occurred in specific fractions. This is an important basis for the application of further analytical techniques in order to perform a more target-oriented analysis aiming at the determination of source and process biomarkers for DOM.     

Observation of edge waves trapped on the continental shelf in the vicinity of Makurazaki Harbor, Kyushu, Japan

Sea-bottom pressure gauges were used to measure sea levels at two points on the shelf off the southern coast of Satsuma Peninsula, Kyushu, Japan. Spectral analysis of the observed records and the tide-gauge record of Makurazaki Harbor revealed several predominant common peaks. At the same time, the eigenmodes for the trapped waves on the shelf and inside Makurazaki Bay were obtained numerically using a two-dimensional model, and the periods and the spatial distribution of amplitudes of the proper modes were obtained. A comparison of the calculated modes with the periods and phase patterns of the observed peaks clarified that peaks with periods of 19.5, 16, 13.3, and 12.2 minutes in the shelf region were the modes of standing-edge waves, and the peak with the period of 16 minutes in Makurazaki Harbor was the fundamental mode of the harbor. Among the modes of standing-edge waves, the mode of the period 16 minutes on the shelf had nearly the same period as that of the fundamental mode of Makurazaki Harbor. An analysis of changes of spectral densities of these two modes confirmed that the fundamental mode of the Makurazaki Harbor was induced by this standing-wave mode.     

Three-dimensional modeling of tidal circulation and mixing over the Java Sea

A combination of a three-dimensional hydrodynamic model and in-situ measurements provides the structures of barotropic tides, tidal circulation and their relationship with turbulent mixing in the Java Sea, which allow us to understand the impact of the tides on material distribution. The model retains high horizontal and vertical resolutions and is forced by the boundary conditions taken from a global model. The measurements are composed of the sea level at coastal stations and currents at moorings embedded in Seawatch buoys, in addition to hydrographic data. The simulated tidal elevations are in good agreement with the data for the K₁ and M₂ constituents. The K₁ tide clearly shows the lowest mode resonance in the Java Sea with intensification around the nodal point in the central region. The M₂ tide is secondary and propagates westward from the eastern open boundary, along with a counterclockwise amphidromic point in the western part. The K₁ tide produces a major component of tidal energy, which flows westward and dissipates through the node region near the Karimata Strait. Meanwhile, the M₂ tide dissipates in the entire Java Sea. However, the residual currents are mainly induced by the M₂ tide, which flows westward following the M₂ tidal wave propagation. The tidal mixing is mainly caused by K₁ tide which peaks at the central region and is consistent with the uniform temperature and salinity along the vertical dimension. This mixing is expected to play an important role in the vertical exchange of nutrients and control of biological productivity.     

半封闭海湾共振周期计算与研究

三门湾海域水波共振现象较为显著,容易引发海洋灾害。针对三门湾这一典型半封闭海湾,分别从理论推导和数值模拟两方面研究了三门湾的共振周期。研究发现:理论推导结果可以用于对海湾整体共振周期的粗略估计,数值模拟结果更加精确,也能充分反映海湾内不同水域的共振特征。数值模拟结果表明,三门湾内各水域普遍存在3~4个共振周期模态,湾内各水道第一模态和第三模态共振周期数值解与理论值较为接近,石浦水道与外海相联通,共振周期不显著。三门湾内各水域共振周期第一模态对应的振幅最大,然后依次递减,但湾顶的青山港、蛇蟠水道第二模态共振周期及对应的振幅值与第一模态相差较小,基本呈现双峰结构。共振振幅由湾外向三门湾顶部逐渐增加,尤其是湾顶处振幅增益比较显著。通过本研究可以为三门湾的防灾减灾提供科学依据。     

台湾东北部地形共振对M2内潮演化影响的数值模拟研究

<正>压潮流、突变海底地形和稳定层结结构是内潮生成的主要因素。台湾东北部海域地形结构多变,正压潮流较强,形成复杂的内潮场。本文通过ROMS(Regional Ocean Modeling System)模式,研究了台湾东北部海域两源区共振条件下内潮的生成、传播和耗散混合过程。结果表明:在台湾东北部海域,Mien-Hua海底峡谷与I-lan海脊是M2内潮重要的生成源区,源区之间的距离与M2内潮水平波长相当,满足共振条件并增强了内潮的生成和耗散。本文通过敏感性试验,探讨模式水平分辨率与不同季节的层化对源区共振过程的影响,数值实验表明,高分辨率的数值模式可以更好地刻画小尺度的地形结构,对复杂地形条件下内潮生成和传播过程的数值模拟十分必要;不同季节的海水层化结构在本质上不改变两源区间的共振关系,但层化强弱对内潮能量的生成具有一定程度的影响。     

深吃水半潜式平台参数共振研究

深吃水半潜式平台(deep draft semi-submersible,DDS)作为一种新型海洋结构物,既继承了传统半潜式平台的优点,又改善了传统半潜式平台垂荡运动性能差的不足。但随着吃水的增加,DDS纵摇运动参数共振也成为一个不容忽视的问题。当DDS纵摇运动固有周期和垂荡运动周期满足一定关系时,纵摇运动将发生参数共振,纵摇角度将会显著增大。研究推导了DDS纵摇运动方程,并简化为标准的马修方程,运用希尔无穷行列式法求解马修方程,得到了含阻尼的马修稳定性图谱,并结合具体算例讨论了不同参数对DDS纵摇运动参数共振问题的影响。研究表明:深吃水半潜式平台的参数共振,是设计人员在设计之初必须考虑的问题;通过合理选取平台的系统参数,可以有效避免纵摇运动参数共振现象的发生。     

Viscosity and nonlinearity effects on the forces and waves generated by a floating twin hull under heave oscillation

Nonlinear hydrodynamics of a twin rectangular hull under heave oscillation is analyzed using numerical methods. Two-dimensional nonlinear time-domain solutions to both inviscid and viscous problems are obtained and the results are compared with linear, inviscid frequency-domain results obtained in [26] to quantify nonlinear and viscous effects. Finite-difference methods based on boundary-fitted coordinates are used for solving the governing equations in the time domain [2]. A primitive-variables based projection method [6] is used for the viscous analysis and a mixed Eulerian–Lagrangian formulation [11] for inviscid analysis. The algorithms are validated and the order of accuracy determined by comparing the results obtained from the present algorithm with the experimental results of Vugt [22] for a heaving rectangle in the free surface. The present study on the twin-hull hydrodynamics shows that at large and non-resonant regular frequencies, and small amplitude of body oscillation, the fluid viscosity does not significantly affect the wave motion and the radiation forces. At low frequencies however the viscosity effect is found to be significant even for small amplitude of body oscillation. In particular, the hydrodynamic force obtained from the nonlinear viscous analysis is found to be closer to the linear inviscid force than the nonlinear inviscid force to the linear inviscid force, the reason for which is attributed to the wave dampening effect of viscosity. Since the wave lengths generated at smaller frequencies of oscillation are longer and therefore the waves could have a more significant effect on the dynamic pressure on the bottom of the hulls which contribute to the heave force, the correlation between the heave force and the wave elevation is found to be larger at smaller frequencies. Because of nonlinearity, the wave radiation and wave damping force remained nonzero even at and around the resonant frequencies – with the resonant frequencies as determined in [26] using linear potential flow theory. As to be expected, the nonlinear effect on the wave force is found to be significant at all frequencies for large amplitude of oscillation compared to the hull draft. The effect of viscosity on the force, by flow separation, is also found to be significant for large amplitude of body oscillation.     

岩心核磁共振测量数据处理方法在北黄海东部坳陷中的应用

岩心核磁共振测井技术具有测量结果准确、对岩石结构无损害的优势,在油田勘探开发中应用越来越广泛。针对北黄海东部坳陷中、新生代有岩心核磁共振的资料进行解谱处理方法研究,确定了预处理、解谱和地层参数求取方法与流程,实现了岩心核磁共振资料解谱处理。根据最优解谱方法得到有效孔隙度、可动流体孔隙度、束缚水孔隙度和渗透率等地层参数。     

珠江河口对外海潮波响应的固有频率研究——采用系统控制论方法

采用受控的自回归滑动平均模型(CARMA)分别研究了珠江河口洪、枯季对外海潮波响应的固有频率。枯季采用单输入单输出模型,洪季采用双输入单输出模型,通过验证、检验表明,模型(CARMA)可用于寻求复杂网河对外海潮波响应的固有频率,方法可靠,易操作。通过频率增益响应分析,发现枯季珠江河口的固有频率以浅水分潮频段为主,对应周期介于6~10.0 h之间,枯季固有频率未出现在全日潮波对应的频段。洪季珠江河口的固有频率也以浅水分潮频段为主,珠江河口对浅水分潮的系统增益响应幅值最大。固有频率接近浅水分潮频段的站点,枯季较洪季固有频率减小,对应周期延长。     

Electron tunneling effects on radiative recombination in modulation n-doped ZnSe/BeTe type-II quantum wells

We have studied the cyclotron-resonance absorption and photoluminescence properties of the modulation n-doped ZnSe/BeTe/ZnSe type-II quantum wells. It is shown that only the doped sample shows electron cyclotron-resonance absorption. Also, the undoped sample shows two distinctive peaks in the spatially indirect photoluminescence spectra, and the doped one shows only one peak. The results reveal that the high concentration electrons accumulated in ZnSe quantum well layers from n-doped layers can tunnel through BeTe barrier from one well layer to the other. The electron concentration difference between these two well layers originating from the tunneling results in a new additional electric field, and can cancel out a built-in electric field as observed in the undoped structures.