Modeling the risk of spread and establishment for Asian longhorned beetle (Anoplophora glabripennis) in Massachusetts from 2008-2009

Land managers responsible for invasive species removal in the USA require tools to prevent the Asian longhorned beetle (Anoplophora glabripennis) (ALB) from decimating the maple-dominant hardwood forests of Massachusetts and New England. Species distribution models (SDMs) and spread models have been applied individually to predict the invasion distribution and rate of spread, but the combination of both models can increase the accuracy of predictions of species spread over time when habitat suitability is heterogeneous across landscapes. First, a SDM was fit to 2008 ALB presence-only locations. Then, a stratified spread model was generated to measure the probability of spread due to natural and human causes. Finally, the SDM and spread models were combined to evaluate the risk of ALB spread in Central Massachusetts in 2008–2009. The SDM predicted many urban locations in Central Massachusetts as having suitable environments for species establishment. The combined model shows the greatest risk of spread and establishment in suitable locations immediately surrounding the epicentre of the ALB outbreak in Northern Worcester with lower risk areas in suitable locations only accessible through long-range dispersal from access to human transportation networks. The risk map achieved an accuracy of 67% using 2009 ALB locations for model validation. This model framework can effectively provide risk managers with valuable information concerning the timing and spatial extent of spread/establishment risk of ALB and potential strategies needed for effective future risk management efforts.     

国际油气井钻头进展概述（二）——FuseTek融合钻头与Pexus组合钻头

NOV公司2013年向市场推出FuseTek融合钻头。它将PDC钻头的高钻速与孕镶金刚石钻头的耐磨性结合于一体,适宜于钻进中硬—坚硬、高研磨性地层。FuseTek钻头已广泛应用于刚果、厄瓜多尔、中国、哥伦比亚等国家。应用表明,该新型组合钻头比PDC钻头或牙轮钻头钻进效率提高许多,工作寿命增加1～3倍或更多。Shear Bits公司于2014年推出Pexus组合钻头。该钻头广泛应用于加拿大冰川冰碛物钻进。地层上部硬卵砾石层利用可转动硬质合金齿钻进,下部软砂岩与页岩则运用PDC碎岩。整个冰碛物地层用一个Pexus钻头一钻到底。所述3类组合式钻头（含Kymera钻头）对于未来实现“一个钻头,一口井”的目标有重要意义。     

青蛤(Cyclina sinensis) TRAF6基因克隆及其在Poly I:C胁迫下的免疫应答

对青蛤(Cyclina sinensis)转录组文库筛选并克隆得到青蛤TRAF6(Cs TRAF6)基因的c DNA序列,其开放阅读框为2337bp,编码778个氨基酸,分子量为86.59k Da。Cs TRAF6蛋白包含RING型锌指结构,两个TRAF型锌指结构,coiled-coil结构域以及MATH结构域。经生物信息学及分子系统学分析表明Cs TRAF6属于TRAF6家族的一员。利用实时荧光定量PCR方法检测了Cs TRAF6基因在青蛤各个组织以及在Poly I:C侵染下在血淋巴中的时序性表达情况。结果表明,Cs TRAF6基因在青蛤血淋巴、闭壳肌、鳃、性腺、外套膜和肝脏中普遍表达,其中在血淋巴中的表达水平最高。在Poly I:C刺激后的3h,青蛤血淋巴中Cs TRAF6的表达量迅速升高,在6h达到最大值,说明青蛤的Cs TRAF6基因参与了病毒类似物侵染下的免疫应答反应。     

海带磷酸甘露糖变位酶(PMM)基因的克隆与表达分析

磷酸甘露糖变位酶(PMM)是褐藻胶和岩藻聚糖合成过程中的关键酶之一。本研究利用c DNA末端快速克隆(RACE)技术,获得2条海带PMM基因(Sjpmm1,Sjpmm2)序列。其中,Sjpmm1的开放阅读框(ORF)长759 bp,其编码的Sj PMM1为卤酸脱卤酶(HAD)超家族成员,含252个氨基酸,分子量约为28.51 k Da;而Sjpmm2的ORF长1866 bp,其编码的Sj PMM2属于磷酸己糖变位酶超家族的成员,含621个氨基酸,分子量约为66.49 k Da。海带PMM的二级结构均以?-螺旋为主。进化分析表明,Sjpmm1来自于原始真核生物,而Sjpmm2来源于质体的第一次内共生作用。实时定量PCR分析发现,海带受到高温或低温胁迫时,Sjpmm1和Sjpmm2转录水平上升,以合成岩藻聚糖抵抗环境影响。此外,利用p MAL-c5X载体对Sj PMM1进行体外表达,得到高浓度的可溶性融合蛋白,为后续的Sj PMM功能分析提供基础。     

COPEX和HM2000与APEX型剖面浮标比测试验及资料质量评价

利用船载CTD仪、国外剖面浮标(APEX)和实验室盐度计等标准仪器设备,在西北太平洋海域对2种型号国产剖面浮标(COPEX和HM2000)进行了现场比测试验,并对观测资料质量进行了定性和定量分析与评价.结果表明:(1) COPEX和HM2000型剖面浮标观测的盐度资料均能达到国际Argo计划提出的±0.01的精度要求;(2)HM2000的最小观测深度离海面1 m以内,最大观测深度基本稳定在2 000 m左右,并能保持在1000 m深度附近漂移,而COPEX的最小观测深度在8～9 m之间,最大观测深度则在1 800～1 900 m之间波动,且漂移深度都在600～800 m之间;(3)COPEX和HM2000都获得了70条以上有效观测剖面.总体而言,两种国产剖面浮标观测的温、盐度资料都是可信、可靠的.但试验中暴露的一些问题和不足仍有待不断改进和完善.     

Geotechnical Hybrid Simulation to Investigate the Effects of the Hydraulic Conductivity on One-Dimensional Consolidation Settlement

Difficulties in the prediction of time-distribution of consolidation settlement will be introduced by using the Murayama test embankment case of Japan. In particular, it will be discussed why the prediction of consolidation rate is difficult in multi-layered soil with complex and variable mechanical properties like organic soil or peat. It can be inferred that uncertainties, which are embedded intricately in the consolidation problem as well as given ground condition, would be major causes for consolidation settlement. After that, the author focused on the movement of pore water under the various conditions of hydraulic conductivity in the soils, and how it can affect the time-distribution of the consolidation settlement. For the applied key methodology on the consolidation settlement problem, we propose the hybrid consolidation simulation controlling the movement of pore water with high accuracy and, finally, the aim of this article is to discuss the methodological approaches obtained by the study, including the basic concept and accurate movement of pore water under various conditions of soil layers and hydraulic conductivity.     

Helical probe tests in residual soils of the Appalachian Piedmont

Helical probe tests (HPT) are a quick and economical means for manual field testing of soils to depths of 1.5 m with readings taken at 0.15-m intervals in only 10 min. The equipment is lightweight (only 2 kg) and thus amenable to deploy on initial site reconnaissance explorations, shallow pavement projects, earth retention walls, and/or compaction of fills. Although suitable for use in a variety of geomaterials: sands, silts, clays, and mixed soils, the specific application to residual fine sandy silts and silty fine sands of the Appalachian Piedmont and Blue Ridge geologic provinces is shown here. Existing relationships for converting the measured HPT torque reading to equivalent cone penetration testing (CPT) tip resistances are reviewed, as well as other trends.     

哈萨克斯坦科扎萨伊油田优快钻井技术

哈萨克斯坦科扎萨伊油田钻井地质环境复杂,储层位于盐下,钻遇的地层层系多,地层性质差异大,具多套压力系统。钻井过程中发生地层漏失、坍塌、掉块及盐膏层蠕变等复杂情况,严重影响了钻井时效和钻井安全。针对这些技术难题,开展了油田井身结构、井身质量、提速潜力、高效钻头等分析与优化,提出了预弯曲钻具高倾角地层井斜控制技术、混合钻头减震提速技术,以及PDC-孕镶块复合钻头高研磨性地层提速技术。现场应用取得了较好的效果,平均机械钻速提高50%以上,钻井周期缩短28%。     

SMA复合摩擦阻尼器性能的试验研究

利用形状记忆合金(SMA)的超弹性效应及高阻尼性能,结合传统Pall摩擦型阻尼器的特点,提出了一种SMA复合摩擦阻尼器。在建立阻尼器力学分析模型的基础上,对SMA复合摩擦阻尼器的性能进行了试验研究,分析了位移幅值、加载频率等对阻尼器的等效刚度、单位循环耗能和等效阻尼比的影响,并与理论分析结果进行了对比。研究表明,SMA复合摩擦阻尼器在加卸载循环下会形成比较稳定的滞回曲线,表明这种阻尼器具有良好的耗能能力。     

钢框架-钢筋混凝土剪力墙结构的地震能量反应分析

以17个钢框架-钢筋混凝土剪力墙混合结构为样本,选取结构自振周期T和结构刚度特征值λ作为分析参数,分析它们在不同类型地震动下的地震能量反应。研究表明,混合结构体系在地震作用下总输入能的大小主要与结构的自振周期以及地震动类型有关,剪力墙与钢框架之间的刚度关系对总输入能影响不大;总输入能等效速度谱的形态受地震动类型的影响很大,同一地震动作用下,幅值与等效速度谱值之间基本能够维持线性增长的关系,但随着结构塑性发展的加剧,这种线性增长关系的离散度会变大;在结构自振周期不变化的前提下,结构的滞回耗能比以及底部剪力墙承担滞回耗能的比例都会随结构刚度比的增大而减小。