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161.
The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological
specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine
bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed
by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent
microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration
and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were
flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based
on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is
easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement
of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as
a new tool to study the fine structures of marine bacteria.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
162.
Since the beginning of formation of Proto-Taiwan, the subducting Philippine (PH) Sea plate has moved continuously through
time in the N307° direction with respect to Eurasia (EU), tearing the EU plate. The subducting EU plate includes a continental
part in the north and an oceanic part in the south. The boundary B between these two domains corresponds to the eastern prolongation
of the northeastern South China Sea ocean-continent transition zone. In the Huatung Basin (east of Taiwan), the Taitung Canyon
is N065° oriented and is close and parallel to B. Seismic profiles show that the southern flank of the canyon corresponds
to a fault with a normal component of a few tens of meters in the sediments and possible dextral shearing. Several crustal
earthquakes of magnitude >%6 are located beneath the trend of the Taitung Canyon and focal mechanisms suggest that the motion
is right-lateral. Thus, faulting within the sedimentary sequence beneath the Taitung Canyon is a consequence of underlying
dextral strike-slip crustal motions. As the continental part of the EU slab located north of B has been recently detached,
some subsequent dextral strike-slip motion might be expected within the EU slab, along the ocean-continent transition zone,
which is a potential zone of weakness. We suggest that the dextral strike-slip motion along the ocean-continent boundary of
the EU slab might trigger the observed dextral strike-slip motion within the overlying PH Sea crust and the associated faulting
within the sediments of the Huatung Basin, beneath the Taitung Canyon.
An erratum to this article is available at . 相似文献
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Are invasive species most successful in habitats of low native species richness across European brackish water seas? 总被引:5,自引:0,他引:5
Marjo Paavola Sergej Olenin Erkki Leppkoski 《Estuarine, Coastal and Shelf Science》2005,64(4):738-750
European brackish water seas (Baltic Sea, Black Sea and Sea of Azov, Caspian Sea) are subject to intense invasion of non-indigenous species (NIS). In these seas, salinity is the most important range limiting factor and native species seem to reach a minimum species richness at intermediate salinities. This trend, revealed by Remane in 1934 and later on confirmed by many other scientists, was compared to the salinity range of already established NIS in the European brackish water seas. It turned out that most NIS are well adapted to the salinities holding lowest native species richness, already in their native area, and that NIS richness maximum in brackish water seas occurs in the salinity intervals of native species richness minimum. A predictable pattern in the salinity range of NIS can be used as a tool in initial risk assessment of future invasions in brackish water seas, especially when mapping highly potential donor and recipient areas. A product of empty niches, suitable environmental conditions, and availability of proper vectors might be the most effective predictor for the invasibility of brackish water areas. 相似文献
168.
Experimental studies on the friction coefficient between concrete and the top surface of a rubble mound foundation in China are reviewed. Through comparison of different test results, the development of this research is comprehensively analyzed. An experiment is carried out in the condition similar to prototype. The process curve of friction coefficient with the test block sliding is analyzed and a standard for determination of the friction coetfficient is defined. The variation features of the friction coefficient are analyzed on the basis of the present experimental results and other studies in China. It is shown that the friction coettfficient between concrete and the top surface of a rubble mound foundation decreases with the increase of the foundation pressure, and the friction coettqcient for a very fine leveling bed is smaller than that for a fine leveling bed. 相似文献
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