中国南海一株固氮类芽孢杆菌的筛选和分离鉴定

为了解中国南海海洋自生固氮菌的种类,作者对采集的南海海底淤泥样品进行了固氮微生物的分离、筛选及鉴定。经过土样沸水加热处理,无氮培养基平板初筛后,对分离获得的细菌固氮酶结构基因nif H进行扩增,并对其固氮酶活性进行检测,最终获得一株能够产芽孢的固氮细菌。对该菌株进行生理生化性状测定、16S r DNA序列分析(Gen Bank登录号KJ627376),并基于nif H、16S r DNA系统进化树分析,确定该菌为一株固氮类芽孢菌(Paenibacillus sp.)NH-1。本研究表明固氮类芽孢杆菌在海洋中确有分布,海洋自生固氮菌的多样性远远超出人们之前的认识。     

A Study of the Molecular Cloud S64 with Multiple Lines of CO Isotopes

We report on a study of the molecular cloud S64 with observations at millimeter wavelengths of multiple molecular lines of CO isotopes.A weak outflow is found,and its physical parameters are estimated.The departure of the core of S64 from the S64 HII region indicates that there are still other star formation activities in that region.     

基于3G与3S集成的土地利用执法检查应用

土地卫片执法检查是政府实施土地严格管理的重要手段.本文以绵阳市2009年度遥感土地执法检查为例,选用2008年和2009年两个时相的中巴资源卫星02B遥感图像作为信息源,对3G与3S集成技术在土地利用执法检查中应用方法进行了研究.该方法利用RS获取土地利用变化信息,利用VRS-GPS技术采集变化图斑空间与属性特征信息,...     

Tracing of Peroxidase Activity in Humic Lake Water

An enzyme assay was developed for studies on peroxidase activities in humic lake water. 3,4-Dimethoxybenzyl alcohol (veratryl alcohol, VeraOH) was used as tracer substrate, and peroxidase (EC 1.11.1.7) activity was measured by high-performance liquid chromatography. The chemical stability of VeraOH and its application as peroxidase substrate was tested under light and dark conditions, different hydrogen peroxide (H₂O₂) concentrations and humic matter contents. VeraOH was stable under low UV radiation at in situ conditions in lake water (<0.010...0.25 kJ m^–2 d^–1), laboratory conditions (<0.05...0.30 kJ m^–2 d^–1), and low (1...100 μM) H₂O₂ concentrations. However, peroxides oxidized VeraOH above 1...10 mM H₂O₂ concentration in sterile Millipore-Q and humic lake water. Dark incubations showed little VeraOH oxidation products. The developed peroxidase assay was tested in the growth medium of Phanerochaete chrysosporium and a bacteria isolate (P.M.D. 20.4.3.1) from mesohumic lake Pääjärvi. Peroxidase activities were also measured in natural microbial communities under standard laboratory and under in situ conditions in humic lake water. Incubation times of about 5 to 12 days were usually needed to record significant (P < 0.05) peroxidase activities, in lake waters. In situ peroxidase activities varied in pelagial surface water (0...0.5 m) on a seasonal scale between 74 nmol L^–1 h^–1 and 273 nmol L^–1 (mean: 176 nmol L^–1 h^–1) and within the water column between 110 nmol L^–1 h^–1 and 800 nmol L^–1 h^–1 (mean: 500 nmol L^–1 h^–1) in polyhumic lake Mekkojärvi.     

SharpMap在青海省气象信息网络数据库中的应用

在 .NET环境下开发了基于B/S结构的青海省气象信息网络数据库。该数据库系统由气象信息基础数据库、数据库管理网站及一个空间信息平台构成。气象数据能够实时入库,并具有底图制作、基于地图的数据检索、数据统计和图形绘制等功能,将查询结果叠加在电子地图上显示,直观方便。系统于2009年在青海省气象台投入使用,提高了青海省气象台气象数据共享能力和服务水平,并为青海省气象台的各项业务工作提供了有力的支持。系统采用SharpMap技术实现了空间信息平台,并应用Ajax等技术提高了系统效率,本文对其在网络数据库中的应用     

基于WebGIS的河南省实时天气监测预警系统设计

为了满足河南省气象台的业务需求,实现对实时天气及灾害性天气的监测和预报预警,提出了采用B/S架构,基于WebGIS技术开发河南省实时天气监测预警系统的设计。该系统以Visual Studio 2013为开发平台,基于Microsoft.NET Framework框架,并利用Geographic Information System(GIS)的可视化技术和空间数据的分析处理技术,实现对河南省区域气象信息的实时监测。利用该系统,用户可以进行实时天气、灾害性天气的监测和报警,强对流天气分析以及预报预警产品的制作与发布。     

Metallogenic age and hydrothermal evolution of the Jidetun Mo deposit in central Jilin Province,northeast China: Evidence from fluid inclusions,isotope systematics,and geochronology

The Jidetun deposit is a large porphyry Mo deposit that is located in central Jilin Province, northeast China. The Mo mineralization occurs mainly at the edge of porphyritic granodiorite, as well as the adjacent monzogranite. Field investigations, cross-cutting relationships, and mineral paragenetic associations indicate four stages of hydrothermal activity. To determine the relationships between mineralization and associated magmatism, and better understand the metallogenic processes in ore district, we have undertaken a series of studies incluiding molybdenite Re–Os and zircon U–Pb geochronology, fluid inclusions microthermometry, and C–H–O–S–Pb isotope compositions. The molybdenite Re–Os dating yielded a well-defined isochron age of 168.9 ± 1.9 Ma (MSWD = 0.34) that is similar to the weighted mean ²⁰⁶Pb/²³⁸U age of 173.5 ± 1.5 Ma (MSWD = 1.8) obtained from zircons from the porphyritic granodiorite. The results lead to the conclusion that Mo mineralization, occurred in the Middle Jurassic (168.9 ± 1.9 Ma), was spatially, temporally, and genetically related to the porphyritic granodiorite (173.5 ± 1.5 Ma) rather than the older monzogranite (180.1 ± 0.6 Ma). Fluid inclusion and stable (C–H–O) isotope data indicate that the initial H₂O–NaCl fluids of mineralization stage I were of high-temperature and high-salinity affinity and exsolved from the granodiorite magma as a result of cooling and fractional crystallization. The fluids then evolved during mineralization stage II into immiscible H₂O–CO₂–NaCl fluids that facilitated the transport of metals (Mo, Cu, and Fe) and their separation from the ore-bearing magmas due to the influx of abundant external CO₂ and heated meteoric water. Subsequently, during mineralization stage III and IV, increase of pH in residual ore-forming fluids on account of CO₂ escape, and continuous decrease of ore-forming temperatures caused by the large accession of the meteoric water into the fluid system, reduced solubility and stability of metal clathrates, thus facilitating the deposition of polymetallic sulfides.