再力花/菖蒲生物湿地床去除河水中氮磷的试验

采用再力花和菖蒲构建湿地床,以考察其对污染河水的净化效果。6个月的连续试验表明:在水力负荷为0.24 m3/(m2.d)、植物种植密度大于158株/m2的条件下,2种植物存活率均大于93%,说明植物能适应低污染负荷、高种植密度的无土培养环境;再力花和菖蒲湿地床月均去除率分别为:总氮(TN),48.22%～78.53%和43.23%～72.42%;总磷(TP),77.62%～85.67%和58.07%～80.77%。再力花湿地床对TN、TP的净化效果好于菖蒲湿地床;2种植物吸收N、P含量分别占去除总量的比例:N为44.14%、37.75%,P为73.43%、62.05%。湿地床技术可有效去除来水中的TN、TP,通过植物吸收作用累积N、P含量较高,不同种类的植物构建湿地床对污染河水中N、P去除效果存在显著差异,且硝化反硝化和植物吸收是去除N的主要途径,而植物吸收是去除P的有效手段。     

Ecological significance of alkaline phosphatase activity and phosphatase-hydrolyzed phosphorus in the northern part of Gamak Bay, Korea

We investigated the ecological significance of alkaline phsophatase (APase) and alkaline phosphatase-hydrolyzable phosphorus (APHP) in the northern part of Gamak Bay, Korea. APase activity was detectable throughout the year, and dissolved inorganic phosphorus (DIP) concentration and APase activity are highly correlated and can be regarded as an indicator of DIP-limiting conditions. Also, a strong linear positive correlation between APase activity and Chl a concentration indicated that the major part of APase activity may have been induced by phytoplanktons. The APHP proportion in dissolved organic phosphorus (DOP) was above ca. 30% from winter to spring and below ca. 15% from summer to autumn due to freshwater discharge and uptake by phytoplankton. APHP may play an important role in species competition in coastal area such as northern part of Gamak Bay where DIP is limiting. Thus, APase induction by phytoplanktons may be ecologically significant, allowing dominance by these organisms under DIP-limiting conditions.     

A Methodology for Clustering Lakes in Alberta on the basis of Water Quality Parameters

In this study, a methodology for clustering 18 lakes in Alberta, Canada using the data of 19 water quality parameters for a period of 11 years (1988–2002) is presented. The methods consist of (i) principal component analysis (PCA) to determine the dominant water quality parameters, (ii) cluster analysis techniques to develop the characteristics of the clusters, and (iii) pattern‐match lakes to determine the appropriate cluster for each of the lakes. The PCA revealed that three principal components (PCs) were able to explain ～88% of the variability and the dominant water quality parameters were total dissolved solids, total phosphorus, and chlorophyll‐a. We obtained five clusters for the period 1994–1997 by using the dominant parameters with water quality deteriorating as the cluster number increased from 1 to 5. Upon matching cluster patterns with the entire dataset, it was observed that some of the lakes belonged to the same cluster all the time (e.g., cluster 1 for lakes Elkwater, Gregg, and Jarvis; cluster 3 for Sturgeon; cluster 4 for Moonshine; and cluster 5 for Saskatoon), while others changed with time. This methodology could be applied in other regions of the world to identify the most suitable source waters and prioritize their management. It could be helpful to analyze the natural controlling processes, pollution types, impact of seasonal changes and overall quality of source waters. This methodology could be used for monitoring water bodies in a cost effective and efficient way by sampling only less number of dominant parameters instead of using a large set of parameters.     

沉积物中磷的赋存形态及磷形态顺序提取分析方法

阐述了当前我国水体富营养化的现状,水体的富营养化与水体中磷、氮的浓度以及二者的浓度比值有着密切的关系。水体富营养化时氮和磷的浓度均很高,但没有一个固定的值,是在一个较宽泛的富营养范围内,氮和磷的浓度比值大多在10∶1～20∶1。湖泊沉积物作为湖泊水体中磷的重要蓄积库,是湖泊流域磷循环的重要归属,也是湖泊水体内源性磷的主要来源,沉积物中极少量的稳定磷转化为活性磷就会造成湖泊水体富营养化。文中介绍了沉积物中无机磷形态的分类,评述了欧盟标准局在研制湖泊沉积物磷形态标准物质时比对采用的4种提取方法(Willimas法、Hieltjes-Lijklema法、Ruttenberg法、Golterman法),总结了国内外学者所应用的沉积物中磷的各种形态提取方法和磷各形态的分析测定方法,国内学者应用的方法是在国外学者方法的基础上提出的,阐述了顺序提取方法及其地球化学意义。简要评述了湖泊沉积物中磷的生物释放、物理释放和化学释放三种释放机制,这三种释放机制相辅相成,在一特定的环境条件下某一过程可能占主导地位来控制沉积物磷的释放。最后提出应该加强对沉积物中磷的各种形态的专属提取剂及其提取有效性的分子表征和检测,更加细化对于沉积物中磷形态的分类和创新各种磷形态的分析方法,建立沉积物-水体磷的迁移、循环和转化模型,这对于从根本上解决富营养化问题具有重要意义。     

模拟湿地生境升温条件下湿地底泥——水体磷元素循环特征

气候变暖对全球生态系统产生了广泛的影响,这其中包括与水质安全和水质演变密切有关的湿地磷元素生物地球化学循环.以长三角南区6块磷库与碳库具有显著差异的湿地为研究对象,通过基于微宇宙原理的湿地生境研究平台模拟升温环境,以现实环境温度为对照组,以(5±1)℃的温度为升温组,探讨升温条件下的湿地磷元素生物地球化学循环特征.结果...     

扎龙湿地芦苇沼泽对农田排水中氮、磷净化效果的模拟研究

农田排水已经成为扎龙湿地水环境污染的重要来源之一.采集扎龙湿地芦苇(Phragmites australis)沼泽区土壤和植物样品,开展为期18天的室内模拟试验,以进水中氨氮(NH4+-N)含量为基准,设计了6组实验,研究芦苇沼泽对不同氮、磷含量农田排水中主要污染物的去除效果,及进水在沼泽中的不同停留时间对湿地去污效果...     

镉和汞胁迫对四角蛤蜊（Mactra veneriformis）血细胞的毒性损伤研究

采用透射电镜观察、溶酶体膜稳定性衡量和微核生成率检测的方法,系统研究了室内模拟Cd和Hg污染胁迫对四角蛤蜊血细胞的毒性损伤。结果表明,Cd对四角蛤蜊血细胞超微结构的损伤表现出明显的浓度依赖效应,125μg/L Cd胁迫下血细胞结构损伤程度高于25μg/L Cd胁迫;不同浓度Cd和Hg暴露14天后,各处理组（2μg/L ...     

长江口跨越锋面颗粒磷季节分布变化特征及影响因素

根据2006年夏、冬季以及2007年春、秋季“海监49号”科学考察船的调查数据,分析了长江口跨越锋面区域(31.00°～31.78°N、121.04°～123.99°E)颗粒总磷(PP),颗粒无机磷(PIP),颗粒有机磷(POP)的季节变化和空间分布特征.结果表明,PIP是水体颗粒磷的主要存在形式;受长江径流输沙量的影...     

Photosynthetic efficiency, cell volume, and elemental stoichiometric ratios in Thalassirosira weissflogii under phosphorus limitation

Nutrient limitation is known to inhibit growth and metabolism and to alter elemental stoichiometric ratios in phytoplankton. In this study, physiological changes in Thalassirosira weissflogii were measured under different dissolved inorganic phosphate (DIP) regimes in semi-continuous cultures to revisit the utility of the Redfield ratio for assessing nutrient limitation. The results showed that cell size increased with decreasing DIP availability. In the P-depleted treatment (f/2-P) the cell size was 1.48 times larger than that in the P-limited (f/100) treatment and 2.67 times larger than that in the P-saturated treatment (f/2 and f/10). The fucoxanthin to chlorophyll a ratio (Fuco/chl a) was relatively stable (about 0.3) in P-saturated cultures and was 10 times higher than that in P-limited and P-depleted cultures. During the experimental period, the photosynthetic efficiency index, ?F/Fm′, was relatively stable at ~0.50 in the P-saturated cultures, but quickly declined with decreasing DIP availability. Although cellular P content showed a significant difference between the P-saturated culture (1.6 pg/cell) and the P-limited culture (0.7 pg/cell), the N/P ratio in T. weissflogii did not show a trend with DIP availability and fluctuated slightly around 25. Our results suggest that cell division in T. weissflogii is not strictly size-gated but is probably regulated by a biochemical, and hence, an elemental stoichiometric ratio threshold, and that deviation of the cellular N/P ratio from the Redfield ratio is not a reliable indicator of algal nutrient stress.     

Harmful algal blooms and eutrophication: ��strategies�� for nutrient uptake and growth outside the Redfield comfort zone

While many harmful algal blooms have been associated with increasing eutrophication, not all species respond similarly and the increasing challenge, especially for resource managers, is to determine which blooms are related to eutrophication and to understand why particular species proliferate under specific nutrient conditions. The overall goal of this brief review is to describe why nutrient loads are not changing in stoichiometric proportion to the "Redfield ratio", and why this has important consequences for algal growth. Many types of harmful algae appear to be able to thrive, and/or increase their production of toxins, when nutrient loads are not in proportion classically identified as Redfield ratios. Here we also describe some of the physiological mechanisms of different species to take up nutrients and to thrive under conditions of nutrient imbalance.