基于18S和ITS-5.8S rDNA基因序列的白色霞水母(Cyanea nozakii)的分子鉴定与检测

采用通用引物PCR扩增法,测定了辽东湾海域的白色霞水母(Cyanea nozakii)螅状体、碟状体及水母体的18S以及ITS-5.8S r DNA序列,同时利用Gene Bank数据库中已有同源序列对其进行序列分析及系统分析。结果显示,白色霞水母3个个体的18S和ITS-5.8S r DNA序列完全一致。白色霞水母样品的ITS-5.8S r DNA序列与Gen Bank中未知真核生物的序列高度相似(≥99%),推测该物种可能是早期发育阶段(卵、浮浪幼虫或碟状体)的白色霞水母样品。霞水母属不同种间18S r DNA序列经比对后同源序列长度为1709bp,多态位点数33个;比对后ITS1同源序列长度为368bp,其中变异位点203个,简约信息位点数178个,单变异位点21个。基于18S r DNA基因序列的霞水母属种内和种间平均遗传距离分别为0、0.008,而基于ITS1序列的霞水母属种内和种间平均遗传距离分别为0.019、0.284。基于ITS1的种间遗传距离是种内遗传距离的15倍,适合于进行物种鉴定。NJ系统树的结果也表明同种的不同个体各自聚枝,其聚类结果大致与形态分类吻合。研究表明,ITS基因片段在霞水母不同种间变异较大,更适于大型水母种类鉴定、检测及属内种间水平的系统进化研究。     

日本囊对虾(Marsupenaeus japonicus)秋繁仔虾形态表型与抗流性能间的相关性

于水温(18.0±1.0)°C、盐度20、pH 8.1±0.2条件下,以水流为胁迫因子(时长1min),以日本囊对虾秋繁同生群仔虾[总长(7.643±0.639)mm]为实验对象,以溢水口(实验初始时刻仔虾放置处)为起点,按等距离间距法将自制的水流测定装置(总长1m)依次划分为A(0—25cm)、B(25—50cm)、C(50—75cm)、D(75—100cm)和E(100cm)等五个区段,在确认实验终了时刻分布于A区段内仔虾数量占实验仔虾总数5%的水流速度为0.823cm/s后,以此为实验流速,借助显微扫描像素测量技术和多元分析方法定量研究了A、B、C、D、E实验群体(依次为实验终止时刻分布于A、B、C、D、E区段内的仔虾)个体间形态比例性状间的差异。结果表明:(1)在所涉15项形态测量指标中,各实验群体间均无显著差异(P0.05)的形态性状共计8项,依次为X_4(眼径)、X_5(头胸甲长)、X_6(头胸甲高)、X_7(第一腹节长)、X_8(第二腹节长)、X_9(第三腹节长)、X_~(10)(第四腹节长)和X_14(腹节高);(2)在所涉17项形态比例指标中,实验群体间均无显著差异(P0.05)的形态比例性状共计7项,依次为C_2(额剑长/总长)、C_9(第五腹节长/总长)、C_(10)(尾节长/总长)、C_(11)(尾扇长/总长)、C_(15)(头胸甲高/头胸甲长)、C_(16)(腹节高/第一腹节长)和C_(17)(尾节高/尾节长);(3)经主成分分析,提取到的5个特征值均大于1的主成分,累计贡献率达80.795%,其中第1主成分39.561%,其载荷绝对值大于0.5的主要影响变量占形态比例性状总数的47.059%;(4)将A实验群体定义为水流胁迫处理选留群,B、C、D、E实验群体统归为水流胁迫处理淘汰群。采用逐步判别法,以判别贡献率较大的C_1(第一触角柄长/总长)、C3(眼径/总长)、C7(第三腹节长/总长)、C13(额剑长/头胸甲长)为自变量,所建的Fisher分类函数方程组可较清晰地区分淘汰群和选留群个体,其中选留群的判别准确率P_1、P_2分别为98%和84.85%,淘汰群的判别准确率P_1、P_2分别为82.25%和97.63%,两者综合判别准确率为90.12%。综上可知,借助形态表型分型可实现日本囊对虾仔虾不同抗流性能群体间的筛选。     

春季我国不同纬度河口浮游动物群落变化趋势

根据2010-2012年春季由南向北河口海域：北仑河口、九龙江口、瓯江口、长江口和灌河口的浮游动物调查资料;通过比较不同纬度河口浮游动物群落的生物多样性指数（H''）、物种更替率（R）和群落相似系数（S）;探讨了河口浮游动物群落特征沿着不同纬度变化的趋势。结果表明;自南向北5个河口可以划分为3个不同的浮游动物区系。北仑河口和九龙江口为南部亚热带区系;位于亚热带海域;以亚热带种为主;H''分别为1.01和2.18;两河口之间R值为65.52%;S值为51.28%;瓯江口和长江口为中部过渡区系;位于亚热带和温带过渡海域;亚热带种和暖温带种是主要出现的种类;H''分别为1.31和1.86;两河口之间R值为58.33%;S值为58.82%;灌河口单独为北部温带区系;位于温带海域;暖温带种是主要出现的种类;H''值为1.19;与其余河口物种更替率均高于80.00%;群落相似系数均低于32.00%;表明群落结构由南到北的变化非常显著。线性回归结果表明：我国不同河口之间浮游动物的物种更替率与纬度差（两河口所处纬度的差值;δN）呈正相关关系（R=0.026ΔN+0.608;p=0.002）;群落相似系数与纬度差呈负相关关系（S=0.034ΔN+0.578;p=0.001）。春季;我国不同纬度河口浮游动物群落结构的变化;主要是因为影响各河口的水温和水团存在较大差别所致。     

SoFISHticated policy – social perspectives on the fish conflict in the Northeast Atlantic

Ecosystem changes currently question the traditional allocation of fishing rights and quotas in the fishery of Northeast Atlantic mackerel and Norwegian spring-spawning herring in the Northeast Atlantic. Variability in the distribution of these highly migratory species escalated in a political conflict between member states of the European Union, Iceland, the Faroe Islands and Norway, which is a driving force for unsustainable fishery. The aim of this paper is to investigate this conflict by outlining the social understandings of diverse stakeholders by using the Q methodology. The method reduced the complexity of numerous opinions, detected four distinct perspectives and simultaneously categorised the participating stakeholders. Although the perspectives differ in various elements, the protection of economic interests seems to dominate over the quest for sustainability. The call of all stakeholders in this study to clarify the fishing rights in the Northeast Atlantic reveals a clear deficiency of the current international fishery management in handling abrupt ecological changes and the necessity to acknowledge this as a complex adaptive system.     

岬湾海滩剖面中长期变化特征及其控制因素——以青岛石老人海滩为例

了解海滩剖面变化可以更好地理解海滩动态过程。利用2007年5月~2014年12月近8 a青岛石老人海滩剖面的实测资料,计算剖面各段单宽体积变化量及后滨宽度,结合交叉小波和小波相干分析方法,探讨海滩剖面中长期淤蚀变化特征及其控制因素。结果表明,近8 a来海滩剖面表现为侵蚀状态,不同岸段侵蚀程度不同。剖面1岸段侵蚀明显,剖面2和剖面3岸段轻微侵蚀。剖面的变化过程可划分为平稳期、剧变期和微调期3个时期。各时期剖面的季节性变化较复杂,平稳期具冬蚀夏淤的交替变化特征,整体淤蚀量较小;剧变期剖面呈阶段性蚀退,变化幅度相对较大,微调期剖面可能仍处于剧变期的恢复阶段,季节性变化不明显。剧变期和微调期的小波交叉谱和相干谱分析显示,波浪和前滨单宽体积相干性较好,尤其当大于2 m的波高达到10%以上,海滩地形可以和波高变化产生同周期的变化。因此波高变化基本控制了剖面的季节变化。而海滩长周期变化主要受控于风暴潮作用及其漫长的恢复期,沿岸输沙和海平面变化则一定程度上导致了剖面长期侵蚀格局的形成。     

基于灵敏度分析的海洋油气资源BP神经网络预测模型的优化

作者针对BP神经网络结构设计中存在的问题,提出利用灵敏度分析方法对BP神经网络预测模型进行优化。通过BP算法与参数灵敏度分析的结合,寻找网络输入属性与输出属性之间的影响因子;在保证精度的前提下优选网络输入属性,简化网络结构,以增强网络的泛化能力,减少人为主观因素对网络设计的影响。最后以海洋油气资源预测为例,结合实测资料建立BP神经网络预测模型并进行了优化及预测精度评价,表明优化后的模型既能有效提高油气资源预测结果的稳定性,又不损失预测精度。     

海带磷酸甘露糖变位酶(PMM)基因的克隆与表达分析

磷酸甘露糖变位酶(PMM)是褐藻胶和岩藻聚糖合成过程中的关键酶之一。本研究利用c DNA末端快速克隆(RACE)技术,获得2条海带PMM基因(Sjpmm1,Sjpmm2)序列。其中,Sjpmm1的开放阅读框(ORF)长759 bp,其编码的Sj PMM1为卤酸脱卤酶(HAD)超家族成员,含252个氨基酸,分子量约为28.51 k Da;而Sjpmm2的ORF长1866 bp,其编码的Sj PMM2属于磷酸己糖变位酶超家族的成员,含621个氨基酸,分子量约为66.49 k Da。海带PMM的二级结构均以?-螺旋为主。进化分析表明,Sjpmm1来自于原始真核生物,而Sjpmm2来源于质体的第一次内共生作用。实时定量PCR分析发现,海带受到高温或低温胁迫时,Sjpmm1和Sjpmm2转录水平上升,以合成岩藻聚糖抵抗环境影响。此外,利用p MAL-c5X载体对Sj PMM1进行体外表达,得到高浓度的可溶性融合蛋白,为后续的Sj PMM功能分析提供基础。     

Ecosystem services assessment and compensation costs for installing seaweed farms

In a global context of promotion and expansion of blue growth initiatives, the development of activities such as aquaculture calls for the assessment of the potential impacts on biodiversity at different levels and associated services. This paper presents an assessment of the potential impact of the installation of seaweed farms on ecosystem services and the induced compensation costs. Biophysical and socioeconomic indicators have been developed for helping decision makers to select the most suitable locations. The approach considers a multi-criteria approach based on Geographical Information Systems (GIS) and Habitat Equivalency Analysis (HEA). The former is used to obtain biophysical ecosystem services and socioeconomic indicators and the latter to evaluate the costs required to compensate the loss of cultural and provisioning ecosystem services. A case-study in the Normand-Breton (Saint Malo) Gulf, France, illustrates this method through the analysis of hypothetical locations of seaweed farms. Results highlight the differences between alternative locations regarding biophysical constraints (in terms of distance and depth), potential risks of conflicts with existing uses, impacts on habitats and the ecosystem services delivered, and compensation costs. This case-study illustrates the flexibility of this approach which can be further adapted to include other indicators in order to deliver integrated information to coastal planners.     

Assessing potential spatial and temporal conflicts in Washington's marine waters

This paper uses spatial, temporal, and use-intensity data for 27 major marine uses in Washington waters to feature a method for assessing potential use conflicts in a variety of scenarios. The study represents a first step towards quantifying potential conflicts within Washington’s Marine Spatial Planning (MSP) study area by using a cumulative analysis to highlight high- and low-use intensity areas and the novel Marine Potential Conflict Index (MPCI), which incorporates space, time, and intensity of use, to quantify pairwise potential conflicts between uses. About 10% of pairs of uses do not overlap spatially and are likely compatible with one another. Temporally, the number of uses peaks in July and August and falls during January and February. Additionally, the MPCI identified three important use types with a substantial degree of potential conflict: commercial fishing, tribal fishing, and shipping. External data were used in three cases to ground-truth the analysis, as a limited test of its utility in managing any potential conflict. This article assesses the extent of Washington’s existing marine uses along spatial, temporal, and intensity axes, and more broadly provides a straightforward way to examine potential conflicts between marine uses.     

南海某导管架海洋平台倒塌分析

针对在役老龄导管架平台进行倒塌计算分析,确定极限承载力进而评估老龄导管架的安全裕度。采用非线性有限元方法,考虑平台的波流载荷及桩-土的非线性相互作用,利用SACS软件建立导管架整体三维有限元计算分析模型,并用逐步加载的方式,对南海某导管架平台进行了全过程非线性倒塌分析。计算分析表明,该导管架平台极限强度很高,具有较大的安全裕度;导管架倒塌过程呈逐步破坏形式,先是撑杆屈服,造成局部结构破坏,然后是钢桩发生屈服,降低结构承载力,最后节点逐步失效,造成结构倒塌。揭示了导管架平台结构失效倒塌的机理,给出了倒塌分析的可行方法和步骤。