基于网络药理学探讨滋补精血、温肾暖宫药物治疗卵巢功能异常疾病的作用机制

目的：基于网络药理学探讨滋补精血、温肾暖宫中药治疗卵巢功能异常疾病的作用机制。方法：采用TCMSP平台获取具有滋补精血、温肾暖宫作用的桑椹、覆盆子、小茴香的活性成分和靶点;网络药理学方法涉及“药物-成分-靶点”网络图的构建和靶点预测。运用京都基因与基因组百科全书（KEGG）数据库确定桑椹、覆盆子、小茴香与卵巢功能异常疾病集群网络的重要信号通路。采用分子对接软件对主要活性成分与核心靶点蛋白进行计算机模拟对接,预测主要成分与核心靶点的结合度。结果：筛选出欧前胡素、β-谷甾醇、槲皮素等10个有效成分,对应于桑椹、覆盆子、小茴香的196个靶点。此外,549个基因与卵巢功能异常疾病密切相关,其中87个与桑椹、覆盆子、小茴香的靶点重叠,被认为与治疗相关。KEGG通路富集分析显示桑椹、覆盆子、小茴香主要作用于卵巢功能异常疾病相关的炎症、卵巢类固醇生成及生长激素的合成分泌和作用等信号通路。分子对接结果显示主要活性成分欧前胡素、β-谷甾醇、槲皮素与靶点均能自发结合。结论：具有滋补精血、温肾暖宫作用的桑椹、覆盆子、小茴香通过多组分、多靶点和多途径协同治疗卵巢功能异常疾病,为后续进一步研究桑椹、覆盆子、小茴香的活性成分及作用机制提供了依据。     

罗氏沼虾(Macrobrachium rosenbergii)卵巢发育不同时期转录组分析

为发掘罗氏沼虾(Macrobrachiumrosenbergii)卵巢发育过程中的重要功能基因,采用IlluminaHiSeqTM4000高通量测序平台对罗氏沼虾卵巢发育四个时期卵巢组织进行转录组测序。所测序列经质控、组装后比对到NR、String、Swiss-prot、Pfam、GO、KEGG数据库中注释,并进行差异基因聚类分析。结果显示,四个样品共产生221580604个Cleanreads,拼接获得95379个Unigenes。分别对四个样品测序文库进行两两比较,共检测到6605个差异表达基因,其中显著上调基因2410个,显著下调基因4195个。GO功能分类显示,共有6422条unigenes可分为分子功能、细胞组分、生物学过程3大类59分支,差异基因主要涉及糖类转运、氨基酸合成、酶活性、细胞膜组成等。通过KEGG通路注释,共有8423条unigenes被注释,涉及184种代谢途径,有7个代谢通路与卵巢发育相关,差异基因的KEGG富集结果显示,药物代谢细胞色素P450通路、氨基丁酸神经元突触、鞘糖脂生物合成、氮素代谢等通路富集显著。此外,对SSR与SNP分子标记进行了鉴定。研究结果为进一步探索罗氏沼虾卵巢发育机制提供了基础信息。     

A finite element cable model and its applications based on the cubic spline curve

For accurate prediction of the deformation of cable in the towed system, a new finite element model is presented that provides a representation of both the bending and torsional effects. In this paper, the cubic spline interpolation function is applied as the trial solution. By using a weighted residual approach, the discretized motion equations for the new finite element model are developed. The model is calculated with the computation program complier by Matlab. Several numerical examples are presented to illustrate the numerical schemes. The results of numerical simulation are stable and valid, and consistent with the mechanical properties of the cable. The model can be applied to kinematics analysis and the design of ocean cable, such as mooring lines, towing, and ROV umbilical cables.     

Slow crack growth in minerals and rocks: Theory and experiments

Strength and mechanical behavior of rocks and minerals are modified by aqueous environments. This results in two effects: mechanical and chemical. The chemical effect is investigated from both a theoretical and an experimental point of view. It is shown that a thermodynamic approach leads to a satisfactory understanding of the chemical effect through an extended griffith concept. Predictions of the model have been tested using slow crack growth experiments. The experiments have been performed with a special Double Torsion apparatus which was built for this purpose. The good agreement observed between theory and experiments suggests that subcritical crack growth in rocks is controlled by adsorption onto the crack tip. This result was previously suggested by other authors (Dunning et al., 1984). However, the important consequences of the model are that (1) there should exist a threshold stress below which subcritical crack growth stops, and this threshold depends on the environment; (2) subcritical crack growth and time-dependent phenomena could take place in the crust in a stress interval which could be as high as 50% of the rupture stress.     

海参花营养成分分析及其提取物对人卵巢癌细胞雌二醇分泌的影响

海参花是雌海参的性腺,在海参加工过程中常被作为下脚料,但它含有丰富的营养成分和多种活性因子,本研究检测了海参花的一般营养物质、元素含量以及氨基酸组成,同时,研究了海参花水提取物和乙醇提取物对人卵巢癌OVCAR 3细胞增殖以及对其雌二醇分泌的影响。结果表明,海参花含水量为86.63%,粗蛋白、粗脂质和粗多糖（干重）占比分别为65.06%、14.82%和1.93%。共检测出17种氨基酸,总氨基酸含量为519.09 mg/kg。其中,必需氨基酸含量为197.04 mg/kg。海参花还含有多种对人体有益的元素。海参花提取物对人卵巢癌OVCAR 3细胞的增殖有明显的抑制作用,海参花水提取物、乙醇提取物均可提高细胞培养液上清液中雌二醇的含量,其中乙醇提取物可显著提高上清液中雌二醇的含量（P<0.01）。以上表明,海参花的营养丰富,其提取物能显著促进人卵巢癌OVCAR 3细胞雌二醇的分泌调节,因此具有很高的开发利用价值。     

Analysis of pile subjected to torsion in multi‐layered soil

A new method of analysis of piles in multi‐layered elastic soil subjected to a torque at the head is developed. The differential equation governing the angle of twist in the pile is derived using the variational principles of mechanics. The method of initial parameters is used to obtain closed‐form solutions of the angle of twist and torque in the pile as a function of depth. The inputs required for the analysis are shear moduli of pile and soil, pile geometry and thickness of soil layers. Copyright © 2013 John Wiley & Sons, Ltd.     

Simplified analysis of asymmetric structures with supplemental damping

This study investigated the effects of neglecting off‐diagonal terms of the transformed damping matrix on the seismic response of non‐proportionally damped asymmetric‐plan systems with the specific aim of identifying the range of system parameters for which this simplification can be used without introducing significant errors in the response. For this purpose, a procedure is presented in which modal damping ratios computed by neglecting off‐diagonal terms of the transformed damping matrix are used in the traditional modal analysis. The effects of the simplification are evaluated first by comparing the aforementioned modal damping ratios with the apparent damping ratios obtained from the complex‐valued eigenanalysis. The variation of a parameter that was defined by Warburton and Soni as an indicator of the errors introduced by the simplification is examined next. Finally, edge deformations obtained from the simplified procedure are compared with those obtained from the direct integration of the equations of motion. It is found that the simplified procedure may be used without introducing significant errors in response for most practical values of the system parameters. Furthermore, estimates of the edge deformations, in general, tend to be on the conservative side. Copyright © 2001 John Wiley & Sons, Ltd.     

Inelastic response of one-storey asymmetric-plan systems subjected to bi-directional earthquake motions

The inelastic response of one-storey systems with one axis of asymmetry subjected to bi-directional base motion is studied in this paper. The effect of the system parameters on response is also evaluated: uncoupled torsional-to-lateral frequency ratio, stiffness eccentricity, and yield strength of the lateral resisting elements. The ensemble of earthquake records used consists of 15 two-component strong ground motions. The response to uni-directional excitation is considered first to examine the influence of the system parameters and to serve as a basis to examine the results of the bi-directional case, which are presented in terms of average spectra for bi- over uni-directional lateral-deformation ratios. It is shown that the effect of inelastic behaviour is, on the average, noteworthy for stiff structures, in turn, the same structures are the most affected by the action of bi-directional ground motions. The effect of the relative intensity of the two orthogonal ground motion components is also studied. Copyright © 1999 John Wiley & Sons, Ltd.     

In-plane floor flexibility effects on torsionally unbalanced systems

This paper presents the results of an analytical work addressed to understand the effects of in-plane floor flexibility on torsionally unbalanced (TU) systems subjected to bidirectional firm-soil earthquake records. The study uses a structural system consisting of a linear-elastic diaphragm supported by non-linear frames oriented along two orthogonal directions. The diaphragm is modelled with plane-stress finite elements and frames with stiffness-degrading flexural elements. Results indicate that an increase of in-plane diaphragm flexibility leads to a reduction of frame displacements for systems with initial lateral period of vibration T>0·4 s. For systems with T⩽0·4 s, in-plane floor flexibility can lead to significant frame displacement increments (50 per cent higher). Results show that these variations on displacements decrease for increasing values of both the seismic-force reduction factor and the system initial lateral period. Copyright © 1999 John Wiley & Sons Ltd.