Experimental study on a sampling technique based on a freeze-sediments valve for deep-sea microorganism

Deep-sea microorganism research has become a hot topic in life science, owing to its special value in high pressure and low temperature environment. If not kept the in-situ low temperature and high pressure, it will cause the microorganism to lose its activity and lead to a waste of resources. A freeze-sediments valve presents a promising solution to maintain the low temperature and high pressure in situ, improve the success rate of sampling and shorten the sampling time. A series of experiments were conducted to characterize the freeze-sediments valve, including the sampling time without leakage, adaptation to different sediments and the effect of the length, etc. Most important of all, temperature change of sediments is observed in all experiments. Experimental results indicated that the freeze-sediments valve was formed in 5 min without leakage and the freeze-sediments valve could be applied to different sediments without limitations on sediments type and mineral content. Considering the pressure retaining capacity and formation time of freeze-sediments valve, it was found that the freeze-sediments valve with a length of 60–80 mm has good pressure retaining capacity whose value was 41.3–48.4 Mpa and short formation time which was 5 min. Precooling the sampler could be used to shorten the sampling time in the actual environment application. This sampling technique can play a vital role in maintaining in-situ low temperature at −2 to −4 ° Celsius in all the experiments. According to the tests, the sampling technique based on the freeze-sediments valve had the potential to solve the problem of keeping in-situ temperature, low sampling success rate and long sampling time in the future.     

Characteristics and petroleum origin of the Carboniferous volcanic rock reservoirs in the Shixi Bulge of Junggar Basin,western China

Shixi Bulge of the central Junggar Basin in western China is a unique region that provides insight into the geological and geochemical characteristics of large-scale petroleum reservoirs in volcanic rocks of the western Central Asian Orogenic Belt. Carboniferous volcanic rocks in the Shixi Bulge mainly consist of striped lava and agglomerate, as well as breccia lava and tight tuff. Volcanic rocks differ in porosity and permeability. Striped lava exhibits the highest porosity (average: 14.2%) but the lowest permeability (average: 0.67 × 10⁻¹⁵ m) among the rock types. Primary gas pores are widely developed and mostly filled. Secondary dissolution pores and fractures are two major reservoir storage spaces. Capillary pressure curves suggest the existence of four pore structure types of reservoir rocks. Several factors, namely, lithology, pore structure, and various diagenesis, govern the physical properties of volcanic rocks. The oil is characterized by a high concentration of tricyclic terpane, a terpane distribution of C₂₃ < C₂₁ > C₂₀, and sterane distributions of C₂₇ < C₂₈ < C₂₉ and C₂₇ > C₂₈ < C₂₉. Oil and gas geochemistry revealed that the oil is a mixture derived primarily from P₂w source rock and secondarily from P₁j source rock in the sag west of Pen-1 Well. The gases are likely gas mixtures of humic and sapropelic organic origins, with the sapropelic gas type dominant in the mixture. The gas mixture is most likely cracked from kerogen rather than oils. The Carboniferous volcanic reservoirs in Shixi Bulge share some unique characteristics that may provide useful insights into the various roles of different volcanic reservoir types in old volcanic provinces. The presence of these reservoirs will undoubtedly encourage future petroleum exploration in volcanic rocks up to the deep parts of sedimentary basins.     

Reservoir origin and characterization of gas pools in intrusive rocks of the Yingcheng Formation,Songliao Basin,NE China

In 2013, the first discovery of gas pools in well LS 208 in intrusive rocks of the Songliao Basin (SB), NE China was made in the 2nd member of the Yingcheng Formation in the Yingtai rift depression, proving that intrusive rocks of the SB have the potential for gas exploration. However, the mechanisms behind the origin of reservoirs in intrusive rocks need to be identified for effective gas exploration. The gas pool in intrusive rocks can be characterized as a low-abundance, high-temperature, normal-pressure, methane-rich, and lithologic pool based on integrated coring, logging, seismic, and oil test methods. The intrusive rocks show primary and secondary porosities, such as shrinkage fractures (SF), spongy pores (SP), secondary sieve pores (SSP), and tectonic fractures (TF). The reservoir is of the fracture–pore type with low porosity and permeability. A capillary pressure curve for mercury intrusion indicates small pore-throat size, negative skewness, medium–high displacement pressure, and middle–low mercury saturation. The development of fractures was found to be related to the quenching effects of emplacement and tectonic inversion during the middle–late Campanian. SP and SSP formed during two phases. The first phase occurred during emplacement of the intrusive rock in the late Albian, when the intrusions underwent alteration by organic acids. The second phase occurred between the early Cenomanian and middle Campanian, when the intrusions underwent alteration by carbonic acid. The SF formed prior to oil charging, the SSP + SP formed during oil charging, and the TF formed during the middle–late Campanian and promoted the distribution of gas pools throughout the reservoir. The intrusive rocks in the SB and the adjacent basins were emplaced in the mudstone and coal units, and have great potential for gas exploration.     

新型张力腿式储油处理平台的动力响应分析

针对目前小油田早期生产中存在的不足,提出一种适用于浅水海域的新型张力腿式储油处理平台,该平台主要由储油箱、浮箱、吸力桩、系泊链和甲板结构构成,具备原油处理和储存功能。应用非线性时域耦合分析法,研究了有义波高、平均波周期、水深对该平台的运动响应和系泊链张力的影响。计算结果表明,波高对平台动力响应有较大影响,有义波高每增加0.4 m,平台水平位移相应增加0.408 m,而系泊链力随有义波高的增加呈准线性增大;波周期对平台水平运动影响显著,平均波周期从6.5 s逐级变化至8.5 s时,最大水平位移自3.760 m渐增至5.467 m,而波周期对系泊链力影响较小,变化率一般小于10%;该新型平台对水深大于20 m的浅水海域有良好的动力特性,而对水深小于20 m的海域却表现异常。因此,该新型张力腿式平台能满足水深不小于20 m浅海区的油田早期生产需要。     

陆丰凹陷韩江组旋回地层学分析及天文年代标尺的建立

珠江口盆地为新生代典型的海相盆地,也是我国重要的海上油气生产基地。前期已有很多学者对珠江口盆地的地层划分与对比开展了研究,但研究的精度不够。为了提高珠江口盆地陆丰凹陷韩江组地层划分与对比的精度,选择珠江口盆地陆丰凹陷A、B井韩江组的自然伽马数据序列作为古气候替代性指标,使用频谱分析、滤波等方法进行旋回地层学分析。通过深度域频谱分析和小波分析认为,该套地层中保存了米兰科维奇旋回信号,且主要受405 ka长偏心率周期的影响。利用稳定的405 ka长偏心率周期进行天文调谐,结合古生物地层年代框架,建立起陆丰凹陷“绝对”天文年代标尺;结合碳氧同位素变化曲线,估算出2次碳同位素负漂移和1次碳同位素正向偏移事件的持续时间;利用天文旋回周期计算出陆丰凹陷韩江组的沉积速率,发现沉积速率的变化与海平面变化具有相关性。     

Seasonally Hypersaline Estuaries in Mediterranean-climate Regions

Data collected in three Californian estuaries indicate that hypersaline conditions exist during the dry summers typical of a Mediterranean climate. The generalised seasonal and longitudinal hydrographic structures are described and explained. It is argued that this seasonal hypersalinity is common and that it represents a major class of estuaries. The observed accumulation of salt indicates surprisingly long residence times in small basins which have free exchange with the ocean. This semi-isolation of the inner basin leads to a large build-up or severe depletion of nutrients, pollutants and plankton in these systems. Of concern are the trends to increase pollutant loading in the same systems that are experiencing an increase in residence times owing to freshwater extraction in the watershed.     

琼州海峡铁路轮渡新海湾港区泥沙淤积问题

海南省拟建琼州海峡铁路轮渡。本文通过对海峡南岸新海湾轮渡港址附近自然条件、岸滩演变和输沙特征的分析研究，对不同类型港区布置方案港内开挖区和航道的淤积强度、淤积量等进行了计算，并对不同防护建筑物的有效拦沙时间等进行了探讨和计算，提出了港区类型推荐方案。     

附加电离层延迟约束的实时动态PPP快速重新初始化方法

传统模式下,由于卫星信号中断等原因导致的实时动态PPP重新初始化问题将严重影响PPP的实际应用效果.针对上述问题,本文提出了一种有效的解决思路,首先设计了电离层延迟变化预报模型,并提出预报信息的定权方法,进而将上述预报结果作为虚拟观测值,联合历元间/星间差分观测数据估计得到周跳参数浮点解,并尝试固定,从而实现PPP快速重新初始化.该方法通过实现电离层预报结果权重的自适应确定,改善了参数估计结果的精度,提高了周跳修复成功率及可靠性.利用不同区域和不同时期的大量观测数据分析表明,周跳修复效果与测站位置、太阳活动及中断时长存在密切关系,当中断时间较短（90 s以内）时,均可以获得较高的成功率,平均达到90%以上,由此验证了本方法的有效性;随着中断时长进一步增大,不同地区和太阳活动情形下的修复效果存在显著差异,纬度越低,太阳活动越剧烈,成功率越低.     

Hydrodynamic modeling of flushing time in a small estuary of North Bay, Florida, USA

Freshwater fraction method is popular for cost-effective estimations of estuarine flushing time in response to freshwater inputs. However, due to the spatial variations of salinity, it is usually expensive to directly estimate the long-term freshwater fraction in the estuary from field observations. This paper presents the application of the 3D hydrodynamic model to estimate the distributions of salinity and thus the freshwater fractions for flushing time estimation. For a case study in a small estuary of the North Bay in Florida, USA, the hydrodynamic model was calibrated and verified using available field observations. Freshwater fractions in the estuary were determined by integrating freshwater fractions in model grids for the calculation of flushing time. The flushing time in the North Bay is calculated by the volume of freshwater fraction divided by the freshwater inflow, which is about 2.2 days under averaged flow conditions. Based on model simulations for a time series of freshwater inputs over a 2-year period, a power regression equation has been derived from model simulations to correlate estuarine flushing time to freshwater inputs. For freshwater input varying from 12 m³/s to 50 m³/s, flushing time in this small estuary of North Bay changes from 3.7 days to 1.8 days. In supporting estuarine management, the model can be used to examine the effects of upstream freshwater withdraw on estuarine salinity and flushing time.     

镉和汞胁迫对四角蛤蜊（Mactra veneriformis）血细胞的毒性损伤研究

采用透射电镜观察、溶酶体膜稳定性衡量和微核生成率检测的方法,系统研究了室内模拟Cd和Hg污染胁迫对四角蛤蜊血细胞的毒性损伤。结果表明,Cd对四角蛤蜊血细胞超微结构的损伤表现出明显的浓度依赖效应,125μg/L Cd胁迫下血细胞结构损伤程度高于25μg/L Cd胁迫;不同浓度Cd和Hg暴露14天后,各处理组（2μg/L ...