Rain-Flow and Reverse Rain-Flow Counting Method for the Compilation of Fatigue Load Spectrum

The rain-flow counting method is widely used to compile the fatigue load spectrum. The second stage counting of the rain-flow method is a troublesome process. In order to overcome this drawback, the rain-flow and reverse rain-flow counting method is proposed in this paper. In this counting method, the rule for counting of the rain-flow method is modified, so that the sequence of load-time need not be adjusted. This is a valid and useful method to count cycles and compile the load spectrum and it can be widely used in ocean engineering.     

Prediction of Load Carrying Capacity of Corroded Reinforced Concrete Beam

A novel method for prediction of the load carrying capacity of a corroded reinforced concrete beam (CRCB) is presented in the paper. Nine reinforced concrete beams, which had been working in an aggressive environment for more than 10 years, were tested in the laboratory. Comprehensive tests, including flexural test, strength test for corroded concrete and rusty rebar, and pullout test for bond strength between concrete and rebar, were condueted. The flexural test results of CRCBs reveal that the distribution of surface cracks on the beams shows a fractal behavior. The relationship between the fractal dimensions and mechanical properties of CRCBs is then studied. A prediction model based on artificial neural network (ANN) is established by the use of the fractal dimension as the corrosion index, together with the basic intbrmation of the beam. The validity of the prediction model is demonstrated through the experimental data, and satisfactory resuits are achieved.     

利用牙鲆鳃细胞系分离和培养淋巴囊肿病毒

本文利用牙鲆鳃细胞系进行了养殖牙鲆淋巴囊肿病毒的分离及培养 ,并通过电镜对培养细胞中淋巴囊肿病毒的形态及感染循环进行了初步研究。将病鱼的淋巴囊肿组织无菌滤液接种牙鲆细胞系 ,细胞出现了明显的细胞病变 ( Cytopathic effect,CPE)。电镜观察在培养细胞的胞质中有病毒的包涵体 ,胞质中散在 6角形、5角形或圆形的病毒粒子 ,大小为 10 0～ 140 nm之间。在感染细胞的线粒体中也存在大量的病毒颗粒。     

二色桌片参酶解液对转化细胞的效应

用二色桌片参（Mensamariaintercedens）酶解液处理转化的人胚肺成纤维细胞（NLF）后，细胞生长受到显著抑制，抑制率达64．84％，光镜下细胞透光性加强，铺展较好，细胞核形更为规则，核畸形现象减少；线粒体结构趋于正常；软琼脂集落实验表明，细胞集落形成率由23ｘ10－5下降至12．5ｘ10－5，裸小鼠致瘤实验显示抑瘤率为82．39％，差异显著（p＜5％）。结果提示，二色桌片参酶解液可能逆转转化的HLF细胞的恶性表型，对其有一定的诱导分化作用。     

底部浮泥表层推移速度分布的ADCP—GPS估测方法

ADCP对底跟踪走航观测的流速数据中包含水体底部浮泥、底沙运动信息,对比GPS定位方法算出的水体流速数据可以分离出浮泥相对于GPS定位的运动信号,从而达到对底质推移观测的目的。     

Two-Dimensional Mathematical Model of Tidal Current and Sediment for Oujiang Estnary and Wenzhou Bay

A 2-D mathematical model of tidal current and sediment has been developed for the Oujiang Estuary and the WenzhouBay. This model accomodates complicated features including multiple islands, existence of turbidity, and significant differ-ence in size distribution of bed material. The governing equations for non-uniform suspended load and bed load transport arepresented in a boundary-fitted orthogonal curvilinear coordinate system. The numerical solution procedures along with theirinitial conditions, boundary conditions, and movable boundary technique are presented. Strategies for computation of thecritical condition of deposition or erosion, sediment transport capacity, non-uniform bed load discharge, etc. are suggested.The model verification computation shows that, the tidal levels computed from the model are in good agreement with the fielddata at the 18 tidal gauge stations. The computed velocities and flow directions also agree well with the values measuredalong the totally 52 synchronously observed verticals distributed over 8 cross sections. The computed tidal water throughputsthrough the Huangda‘ao cross section are close to the measured data. And the computed values of bed deformation fromYangfushan to the estuary outfall and in the outer-sea area are in good agreement with the data observed from 1986 to 1992.The changes of tidal volumes through the estuary, velocities in different channels and the bed form due to the influence of thereclamation project on the Wenzhou shoal are predicted by means of this model.     

高精度CTD剖面仪电导率传感器的研究和实验

高精度CTD剖面仪是海洋863项目的重要组成部分，是海洋调查和水文观测必不可少的仪器，本文详细论述了CTD剖面仪中电导率传感器的研制方法和过程，并给出传感器现已达到的水平，提出还需要进一步进行的工作和深入研究的问题。     

An Analysis on Limit Load for Corroded Submarine Pipelines

1 .Introduction Submarine pipelines are one of the safest and the least environmentally disruptive means for ma-rine gas or oil transport . With the development of exploitation of submarine oil or gas ,the safety ofsubmarine pipelines has becomethe major …     

Application of Atomic Force Microscopy to Observation of Marine Bacteria

The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as a new tool to study the fine structures of marine bacteria. This revised version was published online in July 2006 with corrections to the Cover Date.     

Treatment of Hydrodynamic Loading for Random Sea State

- In order to employ cost effective frequency domain analysis for off-shore structures treatment of hydrodynamic loading is essential. Drag and inertia dominated, resonating and antiresonating cases under random sea states are analyzed to highlight the implications and relative merits of four salient linearization techniques.