近年来,海洋微藻脂肪酸组成的研究及其应用越来越受到国内外科学家的重视。高度不饱和脂肪酸(PUFA),特别是长链的n-3 PUFA,如二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对海洋动物和人类都具有营养学和医学上的价值。在海洋微藻的培养过程中,光照强度是海洋微藻生长的条件之一,光线的明与暗,光度的强与弱,不仅对微藻的生长速率、产量有影响,而且对其脂肪含量和脂肪酸的组成也有影响(Teshima et al.,1983;Thompson et al.,1990; Renaud et al., 1991)。
作者在以往研究的基础上,选择了3种有代表性的海洋微藻:(1)小球藻Chlorella sp-2(李荷芳等,1999),此藻脂肪酸中EPA含量高、且不含DHA;(2)球等鞭金藻(Isochrysis galbana),该藻DHA含量较高,但几乎不含EPA;(3)前沟藻(Amphidinium sp.)的EPA、DHA含量均高。将以上3种微藻作为原料,在不同的光照强度下进行培养,测定并分析藻体中的脂肪含量和脂肪酸组成的变化,从而了解光强对海洋微藻脂肪含量及其脂肪酸组成的影响。 相似文献
The mixing agents and their role in the dynamics of a shallow fjord are elucidated through an Eulerian implementation of artificial tracers in a three-dimensional hydrodynamic model. The time scales of vertical mixing in this shallow estuary are short, and the artificial tracers are utilized in order to reveal information not detectable in the temperature or salinity fields. The fjord's response to external forcing is investigated through a series of model experiments in which we quantify vertical mixing, transport time scales of fresh water runoff and estuarine circulation in relation to external forcing.Using age tracers released at surface and bottom, we quantify the time scales of downward mixing of surface water and upward mixing of bottom water. Wind is shown to be the major agent for vertical mixing at nearly all depth levels in the fjord, whereas the tide or external sea level forcing is a minor agent and only occasionally more important just close to the bottom. The time scale of vertical mixing of surface water to the bottom or ventilation time scale of bottom water is estimated to be in the range 0.7 h to 9.0 days, with an average age of 2.7 days for the year 2004.The fjord receives fresh water from two streams entering the innermost part of the fjord, and the distribution and age of this water are studied using both ageing and conservative tracers. The salinity variations outside this fjord are large, and in contrast to the salinity, the artificial tracers provide a straight forward analysis of river water content. The ageing tracer is used to estimate transport time scales of river water (i.e. the time elapsed since the water left the river mouth). In May 2004, the typical age of river water leaving the fjord mouth is 5 days. As the major vertical mixing agent is wind, it controls the estuarine circulation and export of river water. When the wind stress is set to zero, the vertical mixing is reduced and the vertical salinity stratification is increased, and the river water can be effectively exported out of the fjord.We also analyse the river tracer fields and salinity field in relation to along estuary winds in order to detect signs of wind-induced straining of the along estuary density gradient. We find that events of down estuary winds are primarily associated with a reduced along estuary salinity gradient due to increased surface salinity in the innermost part of the fjord, and with an overall decrease in vertical stratification and river water content at the surface. Thus, our results show no apparent signs of wind-induced straining in this shallow fjord but instead they indicate increased levels of vertical mixing or upwelling during down estuary wind events. 相似文献
Variations in elemental and isotopic ratios of suspended particulate matter (SPM) were investigated in the Guayas River Estuary Ecosystem (GREE) that empties into the Gulf of Guayaquil, Ecuador. Detritus in the system was identified on the basis of extremely high carbon:chlorophyll aratios (>1000). This material had mean δ13C of −26·4±0·3, δ15N of +4·8±0·2, and (C:N)atomicof 14·1±0·9. The isotopic data were comparable to measurements reported for fresh and degrading mangrove leaves, whereas the elemental ratio was comparatively enriched in nitrogen. Isotope measurements of SPM throughout the GREE were more similar to values for riverine material and detritus compared with that for the coastal end-member. Values indicative ofin situproduced algae, sewage and shrimp pond effluent were only found at selected sites. Bacterial bioassays, which were used to document potential sources of dissolved organic matter in the GREE, were isotopically similar to SPM. This correspondence coupled with the relatively low (C:N)aof SPM could be explained by bacterial immobolization of nitrogen onto detritus. Finally, tidal variations of (C:N)aand δ13C at a brackish mangrove site were similar in magnitude to spatial variations encountered throughout the GREE. Based on these results, the authors caution that care must be taken when samples are taken for food-web studies in these systems. 相似文献
Abstract-Heat content of the upper layer above the 20℃ isotherm in the tropical Pacific Ocean isestimated by using the sea temperature data set with a resolution 2°latitude×5°longitude (1980~1993)for the water depths (every 10 m) from 0 m to 400 m, and its temporal and spatial variabilities are an-alyzed. (1) The temporal variability indicates that the total heat in the upper layer of the equatorial Pa-cific Ocean is charcterized by the interannual variability. The time series of the equatorial heat anomaly5 months lead that of the El Nino index at the best positive lag correlation between the two, and theformer 13 months lag behind the latter at their best negative lag correlation. Therefore the equatorialheat content can be used as a better predictor than the El Nino index for a warm or cold event. In addi-tion, it is also found that less heat anomaly in the equator corresponds to the stronger warm events inthe period (1980~1993) and much more heat was accumulated in the 4 years including 1992/1 相似文献
A fluorescent sand-tracer experiment was performed at Comporta Beach (Portugal) with the aim of acquiring longshore sediment transport data on a reflective beach, the optimization of field and laboratory tracer procedures and the improvement of the conceptual model used to support tracer data interpretation.
The field experiment was performed on a mesotidal reflective beach face in low energetic conditions (significant wave height between 0.4 and 0.5 m). Two different colour tracers (orange and blue) were injected at low tide and sampled in the two subsequent low tides using a high resolution 3D grid extending 450 m alongshore and 30 m cross-shore. Marked sand was detected using an automatic digital image processing system developed in the scope of the present experiment.
Results for the two colour tracers show a remarkable coherence, with high recovery rates attesting data validity. Sand tracer displayed a high advection velocity, but with distinct vertical distribution patterns in the two tides: in the first tide there was a clear decrease in tracer advection velocity with depth while in the second tide, the tracer exhibited an almost uniform vertical velocity distribution. This differing behaviour suggests that, in the first tide, the tracer had not reached equilibrium within the transport system, pointing to a considerable time lag between injection and complete mixing. This issue has important implications for the interpretation of tracer data, indicating that short term tracer experiments tend to overestimate transport rates. In this work, therefore, longshore estimates were based on tracer results obtained during the second tide.
The estimated total longshore transport rate at Comporta Beach was 2 × 10− 3 m3/s, more than four times larger than predicted using standard empirical longshore formulas. This discrepancy, which results from the unusually large active moving layer observed during the experiment, confirms the idea that most common longshore transport equations under-estimate total sediment transport in plunging/surging waves. 相似文献